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Citations for GE Life Sciences :
3651 - 3700 of 4188 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... coli supernatant was directly applied onto a 1-ml Ni2+ ion chelating poly-histidine affinity column (HisTrap HP, GE Healthcare) equilibrated with buffer A (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was isolated by centrifugation at 32,000 rpm for 1 h and incubated with Ni-NTA beads (GE Healthcare) for 1.5 h at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... 0.2 μm) and loaded with a flow rate of 1 ml/min onto a Source 15S 4.6/100PE column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Plant Biology 2022Quote: ... Additional purification was carried out by anion-exchange chromatography on a 1 mL HiTrap Q Sepharose column (GE Healthcare, USA). Yields of the recombinant-produced proenzymes ranged from 0.1 to 2 mg per litre culture ...
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bioRxiv - Pathology 2022Quote: ... High-molecular weight RNAs were separated in 1 % formaldehyde agarose gels and transferred to Hybond-N+ membranes (GE Healthcare, UK) for RNA hybridization in membranes ...
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bioRxiv - Plant Biology 2022Quote: ... Acetonenitrile was then added to 70 % and a 10:1 ratio of magnetic beads (Hydrophilic-Part no: 45152105050250, GE Healthcare plus Hydrophobic-Part no ...
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bioRxiv - Neuroscience 2022Quote: ... were applied for 1 h at room temperature and detected with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences) in Fusion FX7 image and analytics system (Vilber Lourmat).
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in the lysates was determined by Bradford assay against BSA and 1-3mg protein-lysate equivalent was loaded onto 10μL of bead slurry (Dynabeads, GE healthcare) prewashed twice with lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... the independently purified SMC1ACC-EQ/RAD21C and SMC3CC-EQ/RAD21N samples were mixed with a 1:0.9 ratio and loaded onto a Superdex 200 16/600 column (GE Healthcare) equilibrated with the purification buffer (200 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... The amplified PCR product was digested with BamHI and XhoI and ligated into the vector PGEx-6p-1 (GE Healthcare) digested with the same restriction enzymes ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was quenched with 1 μl 14.3 M βME followed by final buffer exchange using size exclusion chromatography (GE Healthcare).
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to Amersham™ Protran® Western blotting membranes, nitrocellulose (pore size 0.2 μm, roll W × L 300 mm × 4 m, pkg of 1 ea) (GE healthcare) overnight 4 °C at 15 V ...
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bioRxiv - Microbiology 2024Quote: ... the supernatant was filtered (0.45 polyvinylidene fluoride membrane, Carl Roth, Germany) and applied onto a 1 ml Nickel-IDA column (GE Healthcare) equilibrated in 50 mM TRIS-HCl pH 7.8 ...
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bioRxiv - Cell Biology 2023Quote: ... The column was run at a flow rate of 0.5 mL min-1 on an AKTAbasic liquid chromatography system (GE Healthcare) coupled to a miniDawn MALS detector and an Optilab refractive index detector (Wyatt Technology) ...
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bioRxiv - Microbiology 2023Quote: The supernatant was filtered with a 0.22 μm filter and was loaded onto a pre-equilibrated HisTrap HP 1 ml column (GE Healthcare) at 0.5 ml/min with a peristaltic pump at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... rabbit serums from day 67 post-inoculation were purified using a 1 ml HiTrap NHS-Activated HP column (GE Healthcare) loaded with the corresponding antigen according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: His-SUMO-FacZ127-145 and GpsB1-75 were thawed from frozen aliquots and separately buffer-exchanged into an identical buffer (20 mM HEPES, pH 7.6, 500 mM NaCl, 1 mM DTT) using PD10 de-salting columns (GE Healthcare). Gel filtration samples were made by mixing GpsB1-75 at 90 μM with SUMO-FacZ127-145 at 30 μM in 500 μL reactions ...
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bioRxiv - Microbiology 2023Quote: ... The soluble fraction was loaded at low speed (0.2 ml/min) at 4°C onto a HisTrap-HP 1 mL affinity column (GE Healthcare) equilibrated with buffer A (20 mM NaPi pH 7.7 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The supernatant was collected by centrifugation at 25,000 g for 1 h and incubated with nickel Sepharose resin (GE Healthcare) at 4 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 mM DTT.) Cascade was further purified by size exclusion chromatography using a Superose 6 increase column (GE healthcare) in SEC buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Each clarified lysate was passed twice through a Ni2+-charged 1 ml HiTrap Chelating HP column (GE Healthcare Life Sciences) which had been equilibrated in Buffer TN (50 mM Tris pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... vivax SAL-1 antigen was prepared from Aotus iRBCs purified by Percoll™(GE Healthcare Bio-Sciences AB, Uppsala, Sweden) cushion (47% ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was cleared by centrifugation (140,000 x g for 1 hr) and bound to a HisTrap FF column (GE Healthcare) in buffer A (50 mM Tris pH 8 ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was cleared by centrifugation (140,000 x g for 1 hr) and bound to a HisTrap FF column (GE Healthcare) in buffer A (50 mM Tris pH 7 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Samples were homogenised by bead beating at 4°C in 500 μl Tris-HCl lysis buffer (100 mM, pH 6.8) containing 1% Protease Inhibitor Mix (GE Healthcare). A full and detailed description of the protocol for 2-dimensional gel electrophoresis (2-DE ...
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bioRxiv - Cell Biology 2023Quote: ... 0.15 M NaCl and 0.05% Tween 20 (TNT) at 46 °C for 1 h followed by a streptavidin-Cy5 (GE Healthcare) detection step ...
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bioRxiv - Biochemistry 2023Quote: ... were injected at flow rate of 0.4 ml min−1 to a Superdex 75 Increase 10/300 GL column (GE Healthcare) equilibrated with buffer containing 20 mM Tris-Cl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The BRCA1RING/BARD1RING and BRCA1RING/BARD1193 constructs were concentrated and further purified using a 120 mL Superdex 75 column on an AKTA FPLC system in SEC buffer (25 mM HEPES-NaOH pH 7.5, 150 mM NaCl, 1 mM DTT; GE Healthcare). For the BRCA1RING/BARD1216 ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with specific peroxidase-conjugated secondary antibodies [sheep anti-mouse IgG HRP-linked (1:1000; NA931, GE Healthcare) and donkey anti-rabbit IgG HRP-linked (1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Biochemistry 2023Quote: Protein samples within the concentration range 0.5-1 mg/mL were injected onto pre-equilibrated Superose 6 10/300 size-exclusion chromatography column (GE Healthcare) in buffer containing 20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were incubated for 1 h with horseradish peroxidase (HRP)-conjugated secondary antibody (dilution 1:10,000) and developed with enhanced chemiluminescence (ECL) reagent (GE Healthcare). All western blots were visualized with a LAS4000 Imaging Station (GE Healthcare) ...
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bioRxiv - Plant Biology 2024Quote: ... NATA2Δ) as well as the full-length NATA2 from Arabidopsis were cloned to the pGEX6P-1 expression vector (GE Healthcare). The plasmids were then transformed into Rosetta 2 (DE3 ...
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bioRxiv - Bioengineering 2024Quote: The RSVF-V-1 and RSV90 Fab were purified by size exclusion chromatography using a Superdex200 26 600 (GE Healthcare) equilibrated in 10 mM HEPES pH 7.5 and 150 mM NaCl and concentrated to ∼20 mg/ml (Amicon Ultra-15 ...
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bioRxiv - Cell Biology 2024Quote: ... at 50°C for 1 hour followed by a light-protected incubation with 55 mM iodoacetamide (GE Healthcare; cat. #RPN6302V) at room temperature for 45 min ...
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bioRxiv - Microbiology 2024Quote: ... non-fat dry milk in TBS-T for 1 h at room temperature in a rocking platform: anti-rabbit or anti-mouse IgG-HRP conjugated antibody from sheep (both 1:10,000 NA934 and NA931 GE Healthcare). Proteins were visualized using ECL or SuperSignal West Femto chemiluminescence reagents (Pierce ...
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bioRxiv - Microbiology 2024Quote: ... The solubilized protein suspension was ultracentrifuged at 100,000 x g for 1 hour before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with a purification buffer supplemented with 20 mM imidazole ...
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bioRxiv - Cell Biology 2020Quote: ... The purifications of these proteins from transformed bacterial cell extract were performed according to manufacturer instructions (GST bulk kit, GE Healthcare Life Sciences). The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences) ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... 1 h at room temperature) and cells were collected by gentle filtration onto polycarbonate membranes (25 mm diameter, 0.22 µm pore size, GE Healthcare Bio-Sciences, USA). Filters for counts of reporter deposition coupled with fluorescence in situ hybridization (CARD-FISH ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were purified from the infected cell culture supernatants by binding to a HiTrap ProteinG HP column (GE Healthcare Life Sciences, Piscataway, NJ) and eluted with 0.1 M citrate ...
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bioRxiv - Microbiology 2020Quote: ... cells were treated with trypsin to remove any virus that had not entered the cell and then cushioned onto Ficoll-Pacque (GE Healthcare Life Sciences) to remove residual virus and debris ...
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bioRxiv - Immunology 2019Quote: Peripheral blood mononuclear cells (PBMC) were isolated from whole blood by Ficoll- Hypaque density gradient centrifugation (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) according to manufacturer-provided instructions ...
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bioRxiv - Microbiology 2020Quote: ... The tissue solution was passed through two cell sieves (100µm then 70µm) and then layered with Ficoll®-Paque Premium (GE Healthcare, Hatfield, United Kingdom). Density gradient centrifugation was carried out at 400g for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: ... the H57-scFV was concentrated with a stirred 10 kDa ultrafiltration cell (PBGC04310, MERCK and purified by means of gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) using an ÄKTA pure chromatography system (GE Healthcare Life Sciences) ...
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bioRxiv - Biochemistry 2020Quote: ... cleared lysates from ARL15A86L expressing Flp-In™ T-REx™ HeLa cells were incubated with streptavidin beads (17-5113-01, 5 ml, GE Healthcare). Trypsin digestion on beads was performed and peptides were collected in water ...
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bioRxiv - Systems Biology 2021Quote: ... Then a microscope slide cover was gently placed onto of the agarose pad and cells were imaged using a DeltaVision inverted epi-fluorescence microscope (Applied Precision, Issaquah, WA). Images were captured using a CoolSnap HD charge-coupled device (CCD ...
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bioRxiv - Neuroscience 2020Quote: ... and hyperphosphorylated 2N4R tau produced in Sf9 cells to LRP1 or the VSP10 domain of SORL1 were assessed using a Biacore 3000 optical biosensor system (GE healthcare Life Sciences) essentially as described (M ...
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bioRxiv - Cancer Biology 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood from healthy volunteers by Ficoll-Paque density centrifugation (Ficoll-Paque PLUS, GE Healthcare Life Sciences) and enriched for CD8+ T cells with the MagniSort™ Human CD8+ T cell Enrichment Kit (#8804-6812-74 ...
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Cytokine and phenotypic cell profiles in human cutaneous leishmaniasis caused by Leishmania donovanibioRxiv - Microbiology 2022Quote: Peripheral Blood Mononuclear Cells (PBMC) were isolated from EDTA blood by density gradient centrifugation using 30% of Ficoll-Paque (GE Life Sciences, country). Cells were incubated at 37°C in 5% CO2 and monocytes were separated by adherence after 24 hours and cultured in complete RPMI1640(Gibco ...
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bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells (PBMCs) were isolated by standard ficoll density gradient centrifugation from 70mL heparinized blood immediately after blood withdrawal (GE Healthcare, Uppsala, Sweden). For the first cohort ...