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Citations for GE Life Sciences :
3401 - 3450 of 5100 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The cleaved protein was concentrated and loaded onto a Superdex 200 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 50 mM Hepes pH 7 ...
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bioRxiv - Immunology 2022Quote: ... the protein-containing supernatant was purified by nickel-affinity and size-exclusion chromatography on an AKTAxpress system (GE Healthcare), which consisted of a 1 mL nickel affinity column followed by a Superdex 200 16/60 gel filtration column ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were then further purified by size-exclusion chromatography using a Superdex 75 300/10 column (GE Healthcare) with running buffer 20 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane (BTP) ...
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bioRxiv - Molecular Biology 2023Quote: The extract (supernatant after high-speed ultracentrifugation) was used for protein purification using the ÄKTA Xpress system (GE Healthcare), employing nickel affinity chromatography on the 5 mL column compatible with ÄKTA ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence microscopy data of intracellular GFP fusion proteins were collected using a DeltaVision microscope system (GE Healthcare Inc., USA) equipped with a pco.edge 4.2 sCMOS camera (PCO ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were concentrated by centrifugation in Vivaspin 20 ultrafiltration devices with a 100,000 molecular weight cut-off (GE Healthcare). Protein concentrations were quantified by UV spectroscopy with an extinction coefficient of 36380 M-1cm-1 (Drp1 ...
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bioRxiv - Biophysics 2023Quote: ... Protein was isolated from the resulting supernatant by affinity chromatography using Ni-Sepharose 6 Fast Flow beads (GE Healthcare), and eluted with a 100 mL linear gradient of column buffer with 500 mM imidazole ...
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bioRxiv - Cancer Biology 2023Quote: ... The precipitated proteins were purified based on nickel affinity purification by AKTA Prime Plus automated purification system (GE Healthcare). The sample passed over a 1 mL Nickel-Sepharose (HisTrap HP ...
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bioRxiv - Cancer Biology 2023Quote: ... and IgGs were then purified from the culture supernatants by Protein A-column chromatography (GE Healthcare, 17-1279-01). We obtained 6 clones as follows ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were removed by centrifugation and proteins were purified from the conditioned media using rProtein A Sepharose (GE healthcare). The purified proteins were buffer-exchanged into a formulation buffer (20 mM His-HAc ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated with SDS-PAGE and subsequently transferred to Amersham Hybond-P PVDF Transfer Membrane (RPN303F, GE Healthcare). Membranes were blocked in 5% milk in Tris-buffered saline with 0.01% Tween 20 and incubated with the antibodies listed in Supplemental Table 4 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining purification steps were carried out at 4 °C using an ÄKTA protein purification system (GE Healthcare/Cytiva) for column chromatography steps ...
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bioRxiv - Biochemistry 2023Quote: ... 200 μL of protein solution were loaded onto a Superdex 200 HR 10/30 gel filtration column (GE Healthcare) with a flow rate of 0.5 ml/min ...
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bioRxiv - Biophysics 2023Quote: ... The untagged protein was purified using gel filtration chromatography (ÄKTA with Superdex-200 increase 10/300 column; GE Healthcare) and equilibrated with storage buffer ...
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bioRxiv - Immunology 2023Quote: ... Unpurified proteins contained in HEK293F filtered supernatants were captured on PGT145-functionalized CNBr-activated sepharose 4B beads (GE Healthcare) by overnight rolling incubation at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: Histone proteins were mixed in stoichiometric amounts and eluted through a HiLoad 16/600 Superdex 200pg column (GE Healthcare) to produce H2A-H2B dimers and (H3-H4)2 tetramers separately 40 ...
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bioRxiv - Immunology 2023Quote: ... Fractions containing recombinant protein were combined and purified by gel filtration on a S200 26/60 column (GE Healthcare) equilibrated with standard TBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were blotted for 80 min (350 mA, room temperature) onto Amersham Protran 0.2 μm nitrocellulose membranes (GE Healthcare). Membranes were blocked in 5% milk in TBS-Tween for at least 2 h and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: Protein samples (4mg/mL; 80 µL) were separated on Superdex 200 Increase 10/300 GL Tricoedn column (GE Healthcare) in 20 mM Hepes containing 150 mM KCl ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were further purified by size-exclusion chromatography using a Superdex 200 column (Cytiva, formerly GE Life Sciences) in buffer B ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were further purified via size exclusion chromatography using a Superdex200 Increase 10/300 GL column (GE Healthcare) in running buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... and the protein in the supernatant was diluted to 1% Triton and incubate with Glutathione Sepharose beads (GE Healthcare) in a batch mode ...
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bioRxiv - Plant Biology 2023Quote: ... Protein bands were quantified by densitometry with ImageQuant TL software (version 7.0 GE Healthcare Life Sciences, Pittsburgh, PA, USA). PsbS abundance was quantified relative to WT based on a dilution series of WT PsbS protein.
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bioRxiv - Molecular Biology 2023Quote: ... The protein was further purified by size exclusion chromatography using a Superose 6 10/300 GL column (GE Healthcare) and eluted in 50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: Interactions between the DMC1 protein and the synthetic BRCA2 peptides were characterized using a VP-ITC Calorimeter (GE Healthcare). The peptides tested were wild type and F2406A variant of the PhePP (F2s6 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE using 4% or 5% polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with mouse monoclonal antibody generated against HA-tag peptide (YPYDVPDYA ...
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bioRxiv - Neuroscience 2023Quote: ... the protein solution was concentrated and further purified utilizing size exclusion chromatography with a SuperdexTM 75 column (GE Healthcare). The protein solution was concentrated to 9.2 mg/mL and used for crystallization.
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bioRxiv - Microbiology 2023Quote: ... Fractions containing predominantly ATeam protein were pooled and applied to a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated with 20 mM tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... Protein TLR827–827-PA-His was purified with IgG beads (Cat#17-0969-01, GE Healthcare, Connecticut, CT, USA) and resuspended in 200 μl of NT2 buffer 66 and used for miRNA-protein co-IP ...
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bioRxiv - Biochemistry 2023Quote: ... The protein fractions were concentrated and finally subjected to Superdex 75 10/300 size exclusion chromatography column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were further purified by gel filtration using a Superose 6 Increase 10/300 GL column (GE Healthcare). Snu71 fragments with residues 1-96 ...
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bioRxiv - Cell Biology 2023Quote: ... soluble proteins were applied to the size exclusion gel filtration column (Superdex 200 Increase 10/300 GL, GE Healthcare), using an Akta P-920 Pump FPLC system (Amersham ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was further purified using size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) using an Akta pure system (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... N-myc proteins were polished using size-exclusion chromatography (SEC) using a Superdex 16/600 S75 column (GE Healthcare). GB1-tagged N-myc proteins were purified in a similar manner with a few alterations ...
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bioRxiv - Cancer Biology 2024Quote: ... supernatants were incubated with 30 µL of protein G Sepharose™ 4 Fast Flow (#17-0618-01, GE Healthcare) under agitation for 4 hours at 4°C ...
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Dynamic regions allosterically connect the USP14 active site with the proteasome interaction surfacebioRxiv - Biophysics 2024Quote: ... Protein was purified as previously described [59] but with a Hiload 16/600 Superdex 200 pg column (GE Healthcare) for size exclusion chromatography (SEC) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 ml protein was loaded onto the analytical size exclusion column Superdex 200 increase 10/300 GL (GE Healthcare) in running buffer (25 mM Tris-HCl ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected after 6 days and purified using a 5-ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5-ml kappa-select column (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... Deglycosylated proteins were purified to homogeneity by size exclusion chromatography using a HiLoad Superdex 75 PG column (GE Healthcare) equilibrated in Dulbecco’s Phosphate buffered saline (DPBS ...
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bioRxiv - Microbiology 2024Quote: ... was used to visualize proteins on an Amersham Imager 600 RGB gel and membrane imager (GE Life Sciences/Cytiva).
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to 0.45 µm pore-size nitrocellulose membranes (GE Healthcare Life Sciences). Membranes were blocked at room temperature for 1 h with Tris-buffered saline (TBS ...
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bioRxiv - Immunology 2024Quote: ... Purification was carried out following manufacturer’s instructions on HiTrap Protein G HP purification column for IgG mAbs (GE Healthcare), and a POROS CaptureSelect IgM-XL Affinity Matrix Column (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Refolded HLA-G2 protein was purified by size exclusion chromatography using HiLoad 26/60 Superdex75 pg column (GE Healthcare) equilibrated with 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... 5 ml of pooled mouse sera was then used to purify IgG using Protein A Sepharose resin (GE Healthcare) and digested for using papain-agarose resin (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by incubation with the corresponding secondary anti- rabbit antibody (GE Healthcare, NA934) or anti-rat antibody (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2021Quote: ... horseradish peroxidase (HRP)-conjugated anti-rabbit IgG antibody (GE Healthcare Japan, Tokyo, Japan), and ECL Prime (GE Healthcare Japan ...
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bioRxiv - Molecular Biology 2022Quote: ... Bound antibodies were detected with the Enhanced Chemiluminescence (ECL) system (GE Healthcare Amersham) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... horseradish peroxidase-linked secondary antibody (donkey monoclonal, 1:2000, GE Healthcare, Bucks, UK) for 45 min ...
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bioRxiv - Genomics 2020Quote: ... The secondary antibody was HRP conjugated Goat anti-Rabbit (NA934, GE Life Sciences). The samples were resolved on 4-12% Bis-Tris NuPAGE (Invitrogen ...