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Citations for GE Life Sciences :
3651 - 3700 of 5100 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Total proteins were separated in a 4–15% SDS–PAGE gradient and transferred to nitrocellulose membrane (GE Healthcare Life Science). Membranes were blocked with 5% (m/v ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified by size-exclusion chromatography on a HiLoad 16/600 Superdex 200 Column (GE Life Sciences).
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bioRxiv - Biophysics 2019Quote: ... The cleaved protein was then purified by a 5 ml HisTrap HP column on an AKTA Avant system (GE Healthcare). The flow through was combined and diluted with buffer C ...
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bioRxiv - Genetics 2020Quote: ... 2-4 mg protein extract was added to 50 μl of Glutathione Sepharose beads (Cat. no. 17527901, GE healthcare Ltd.) equilibrated with 1XPBS and incubated for 10-14 hours at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The proteins were further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare). The selected fractions were pooled and concentrated again for further use.
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bioRxiv - Plant Biology 2021Quote: ... Approximately 1 mg of total cytosolic proteins was injected onto a Superdex® 200 10/300 GL column (GE Healthcare). The SEC elution was performed with the mobile phase [50 mM Hepes/KOH ...
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bioRxiv - Biochemistry 2021Quote: ... with purification of labelled protein away from free label using a desalting Superdex G-25 (PD-10) column (GE Healthcare). FRET was examined using 495 excitation for FITC-HU-A ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μL solution of 1.5 mg/mL protein was injected onto a Superdex 200 Increase 10/300 GL (GE Healthcare) column equilibrated in a buffer of 20 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... Protein-containing fractions were pooled and 1.6 ml protein was subjected to size exclusion chromatography using a Superdex 200 XK16/60 column (GE Healthcare) and equilibrated into 50 mM Tris-HCL pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: The amino acids 77-136 encoding the cytoplasmic region of the Hoka protein were cloned into pGEX-6P (GE Healthcare) to produce a GST (Glutathione S-transferase ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by size exclusion chromatography (Hi-load 16/60 Superdex 200 column, GE Healthcare Life Sciences) in a buffer (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2020Quote: ... S-Trimers were purified to homogeneity by consecutive chromatographic steps including Protein A affinity column using MabSelect PrismA (GE Healthcare) which was preloaded with Endo180-Fc at (3 mg/mL ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... Recombinant His-tagged proteins (MA4376, MA4376D54N and MA4375) were purified with the ÄKTAprime plus system using HisTrap HP (GE healthcare) columns ...
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bioRxiv - Microbiology 2021Quote: ... Purified proteins were concentrated by ultrafiltration and buffer-exchanged into PBS on a 5 ml HiTrap Desalting column (GE Healthcare). The concentration was determined from UV absorbance at 280 nm ...
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bioRxiv - Microbiology 2020Quote: ... the harvested protein was concentrated to approximately 2.0 ml and filtered using a Superdex 200 gel filtration column (GE Healthcare) equilibrated with buffer (20 mM Tris-HCl and 200 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Protein sample were injected into a size exclusion column (Superose™ 6 Increase 5/150 GL, GE Healthcare Bio-Sciences) previously equilibrated with corresponding buffer and eluted directly into the SAXS flow-through capillary cell at a flow rate of 0.2 ml/min ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of protein extracts underwent vacuum filtration through a pre-wet 0.2 μm pore size nitrocellulose membrane (GE Healthcare) for the detection of ubiquitinated protein aggregates or a 0.2 μm pore size cellulose acetate membrane (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Concentrated and filtered protein was analyzed by size-exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with HEPES buffer ...
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bioRxiv - Molecular Biology 2021Quote: Proteins (10 μg) were separated by 10%–12.5% SDS-PAGE and electroblotted onto a 45-μm nitrocellulose membrane (GE Healthcare). Membranes were stained with Ponceau to ensure equal distribution of proteins in each lane ...
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bioRxiv - Immunology 2021Quote: ... IgG and IgM were separated from serum by affinity chromatography using HiTrap Protein G High Performance column (Cytiva, GE Healthcare) and CaptureSelect IgM Affinity Matrix (Thermofisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Eluted protein was concentrated down to 2 mL for size exclusion chromatography with a Superose6 16/600 column (GE Healthcare) in 10 mM Tris pH8 ...
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bioRxiv - Microbiology 2021Quote: ... The resulting protein samples were further purified by size-exclusion chromatography using a Superdex 75 10/300 column (GE Healthcare) or a Superdex 200 10/300 Increase column (GE Healthcare ...
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bioRxiv - Genomics 2021Quote: ... Protein lysates were separated in 12% acrylamide gels and transferred to nitrocellulose blotting membrane (cat.# 10600004-GE Healthcare Life Sciences). The membrane was blocked with 5% skim milk in TBS-T (20 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Genetics 2020Quote: ... followed by purification of the tag-free protein by anion exchange chromatography on a HiTrap Q XL column (GE Healthcare) and nickel affinity chromatography ...
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bioRxiv - Bioengineering 2020Quote: ... Eluted proteins were further purified by size exclusion chromatography on a Hiload 16/600 Superdex 75 pg column (GE Healthcare) in PBS buffer.
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bioRxiv - Neuroscience 2021Quote: ... Recombinant TSP2 protein was purified from CHO cells expressing mouse TSP2 via affinity chromatography with HiTRAP heparin HP (GE Healthcare) following protocols described by Oganesian et al ...
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bioRxiv - Biochemistry 2021Quote: ... The unbound protein was concentrated and further purified via size exclusion chromatography on HiLoad S200 16/60 column (GE Healthcare) in buffer A2 (300 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... equal amounts of protein (30 µg) were run on homemade 10% polyacrylamide gels and transferred on nitrocellulose membranes (GE Healthcare). Membranes were probed with primary antibodies (listed in the table below) ...
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bioRxiv - Microbiology 2022Quote: SEC analysis of single proteins or combinations of the subunits was conducted using AKTA basic 10 FPLC system (GE healthcare) and a Superdex 200 Increase 10/300 SEC column ...
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bioRxiv - Cancer Biology 2022Quote: MSLN complex with VH 3C9 was prepared by injecting the protein mixture to Superdex 75 column (GE Healthcare, Chicago IL), and concentrated to 12 mg/mL ...
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bioRxiv - Biophysics 2022Quote: ... After loading the cell lysate onto a Protein L beads (Capto™ L, GE Healthcare, Cat. no. 17-5478-02) column ...
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bioRxiv - Cell Biology 2022Quote: ... was then loaded to a 5 mL HisTrap Ni-NTA column coupled to AKTA Pure protein purification system (GE Healthcare). The His-SUMO-tagged CAP1 protein was eluted from the nickel column with an imidazole gradient using buffer A and buffer B (buffer A + 250 mM imidazole) ...
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bioRxiv - Biochemistry 2022Quote: ... The complex was applied onto superdex200 increase 10/300 GL column via fast protein liquid chromatography (AKTA Pure, GE Healthcare) with buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... 25 μl of pre-equilibrated protein L beads (Capto™ L resin, GE Healthcare Cat no. 17547802 Cat. no. 17547803) was added and reaction was incubated for 90 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... a semi-dry transfer was done to blot the proteins in a 0.45 μm nitrocellulose membrane (GE Healthcare, Chicago, IL). Proteins were transferred for 90 minutes at 65 mA ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were concentrated and further purified by size exclusion chromatography on a Superdex 75 column (GE Healthcare Life Sciences) pre-equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... the crude lysate proteins were separated by Tricine-SDS PAGE (16%) and blotted onto a PVDF membrane (AmerhamTMHybondTM, 0.2 µM PVDF; GE Healthcare Life Science ...
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bioRxiv - Plant Biology 2022Quote: ... DNA-protein complexes were resolved in a 6% polyacrylamide gel and transferred to a charged Hybond-N+ membrane (GE Healthcare). The membrane was cross-linked by UV (1200 mJ) ...
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bioRxiv - Plant Biology 2022Quote: ... The culture was harvested and the recombinant protein purified using glutathione resin according to the manufacturer’s instructions (GE healthcare, 17075601).
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bioRxiv - Microbiology 2022Quote: ... Fractions containing purified His6-tagged proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer 1 (50mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples containing 10 µg of proteins were filtered through Whatman Cellulose Acetate Membrane Filters with 0.2 µm pore sizes (GE Healthcare), which have very low protein binding capacity ...
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bioRxiv - Immunology 2022Quote: ... The purified protein buffer was exchanged to PBS containing 0.5 mM TCEP using a HiPrep 26/10 desalting column (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Proteins were resolved on 6% SDS-PAGE gel and transferred to a nitrocellulose membrane (0.45 μm pore size, GE Healthcare) by Trans-Blot Turbo system (BioRad) ...
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bioRxiv - Plant Biology 2022Quote: ... Fifty micrograms of proteins were separated by SDS-PAGE and transferred to an Amersham Hybond-P PVDF membrane (GE Healthcare). The membrane was probed with rabbit polyclonal antibodies against maize calreticulin ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein bands were visualised using Amersham ECL Western Blotting Detection Reagent and the ImageQuant LAS4000 luminometer (GE Healthcare Life Sciences). βIII-tubulin relative band intensities were quantified using ImageStudio software (LI-COR Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... and the soluble proteins were isolated by centrifugation (80,000g, 45 min, 4°C) and purified by cation exchange chromatography (HiTrap SP, GE Healthcare) with a gradient of 0-1M NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... The protein was separated from free dye using gel filtration on a Superdex 75 pg 10/300 column (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... The resultant supernatant was filtered using a 0.45 µm filter and the protein was subsequently purified by IMAC on a 5 ml HP HisTrap column (GE Healthcare) according to manufacturer’s information ...