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Citations for GE Life Sciences :
3201 - 3250 of 5100 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... The protein were electrophoresised for 1h at 150V and subsequently transferred onto MtOH activated polyvinylidene fluoride membranes (GE Healthcare). Equal loading was assessed using Ponceau red solution ...
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bioRxiv - Neuroscience 2021Quote: ... The labeled proteins were separated from the free dyes on a homemade Sephadex G50 resin (GE Healthcare, Piscataway, NJ) column in TBS buffer.
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bioRxiv - Molecular Biology 2020Quote: ... proteins were separated by SDS-PAGE and transferred to AmershamTM ProtranTM 0.45 μm nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were probed with primary antibodies at dilutions recommended by the suppliers ...
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bioRxiv - Molecular Biology 2020Quote: ... The ion-exchange chromatography was performed for further protein purification with an anion-exchange column (Source 15Q, GE Healthcare). Then peak fractions were collected and stored at -80 °C before using.
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred from polyacrylamide to nitrocellulose membranes (AmershamTM ProtranTM Premium 0.45 µm NC, GE Healthcare Life sciences, 10600003) by electrotransfer (Bio-Rad laboratories Inc.) ...
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bioRxiv - Immunology 2019Quote: ... after which proteins were transferred to nitrocellulose membranes (Amersham Hybond ECL 0.45 µm Nitrocellulose Membrane, GE Healthcare Life Sciences) or PVDF membranes (Amersham Hybond P 0.45 µm PVDF Membrane ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified on an ion exchange column MonoS and gel filtration column Superose 6 (GE Healthcare). Purified protein was concentrated to ∼20 mg/ml in BRB20 buffer (20mM PIPES ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences). The following antibodies were used ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Biochemistry 2019Quote: ... Protein at 50 µM was injected onto a Superdex 200 10/300 GL size-exclusion chromatography column (GE Healthcare) equilibrated in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... 40 μg of total protein were run on a SDS-polyacrylamide gel and transferred onto nitrocellulose membranes (GE Healthcare). After blocking in 5% (w/v ...
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bioRxiv - Immunology 2019Quote: ... All His-tagged proteins were purified from the cell culture medium using HisTrap Excel column (GE Healthcare, catalog 17371206) and eluted using buffer containing 50 mM HEPES-NaOH ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysate was pre-incubated with 200 μL of rehydrated Protein A Sepharose CL-4B beads (GE Healthcare, Life Sciences) for 15 minutes at 4°C with rocking ...
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bioRxiv - Cell Biology 2019Quote: ... LEM2 proteins were further purified by size exclusion chromatography using the Superdex 75 16/60 column (GE Life Sciences) in storage buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... the desalted protein-containing fractions were pooled and loaded on a Q sepharose FF column XK16/32 (GE Healthcare). The protein-containing fractions in the flow-through of the column were again pooled ...
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bioRxiv - Molecular Biology 2019Quote: Clarified extracts containing hexahistidine fusion proteins were passed through a 5 mL Ni-Sepharose (GE Healthcare #17-5268-01) packed column at 0.5 mL/min using an ÄKTAprime Plus chromatography system ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified extracts containing GST fusion proteins were passed through a 5 mL GSTrap column (GE Healthcare #17-5131-02) at 0.1 mL/min using an ÄKTAprime Plus chromatography system (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: For Western blotting protein samples were resolved by 10% SDS-PAGE and transferred to a PVDF membrane (GE Healthcare) by semi-dry blotting ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The respective IgG2a antibodies were incubated with the PepSpot membrane at concentrations ranging from 0.5 µg/ml to 2µg/ml and detected by protein A-HRP (GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Quality control of the proteins was performed by SDS-PAGE and size exclusion chromatography (Superdex200 10/300GL, GE Healthcare). The full amino acid sequences are presented in Supplementary Figure 2 ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B and the cleaved topo VI collected in the flow through ...
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bioRxiv - Genetics 2021Quote: ... All protein and DNA localization was done with images that had been deconvolved using SoftWoRx (Applied Precision, GE Healthcare).
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bioRxiv - Genetics 2020Quote: ... Samples were separated on 10–12% SDS-PAGE gels and proteins were transferred to Protran nitrocellulose membranes (GE Healthcare), after which membranes were stained with 0.3% w/v Ponceau S in 3% v/v acetic acid and digitized ...
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bioRxiv - Genetics 2021Quote: ... All protein and DNA localization was done with images that had been deconvolved using SoftWoRx (Applied Precision, GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: For NMR structure determination the proteins were buffer exchanged using PD-10 columns (Sephadex G-25 M, GE Healthcare) or via gel filtration chromatography in the final purification step to 20mM MES 6.5 ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were then separated by SDS-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose Amersham Protran membrane (GE Healthcare). Membranes were blocked at room temperature with either 5% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... and further purified using size exclusion chromatography (SEC) on an AKTA pure 25L protein purification system (GE Healthcare, USA) in buffer C (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Further purification of the proteins was achieved using a Superdex 200 increase 10/300 size exclusion column (GE Healthcare) in 50 mM HEPES/NaOH pH7.8 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed using 0.1% TBS-T and proteins were detected using ECL or ECL Select (GE Healthcare, USA) on ChemiDoc XRS+ System (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was additionally purified by ion-exchange chromatography on a “Mono S 5/50 GL” column (GE healthcare) at 0-100% B gradient over 15 column volumes at 0.5 ml/min flow rate using 20 mM sodium phosphate buffer (pH 6.0 at 25 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Enc:EncFtn protein complex sample was loaded onto a 20 ml anion exchange column (Hi-Prep Q HP, GE Healthcare) pre-equilibrated with Buffer QA (50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by size exclusion chromatography using an S75 or S200 16/60 column (GE Healthcare) equilibrated in running buffer (10 mM Tris/HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was applied to size-exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE healthcare) in gel filtration buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were further purified by gel filtration using a Superdex 200 Increase 10/300 GL column (GE Healthcare). nsp7 and nsp8 were purified in SEC-150 buffer ...
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bioRxiv - Biochemistry 2020Quote: Soluble protein was manually applied at room temperature to a 5 mL HiTrapQ anion exchange column (GE Healthcare # 29051325) that had been pre-equilibrated in buffer B ...
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bioRxiv - Biophysics 2021Quote: ... The protein was further purified by size exclusion chromatography using a Superose 6 column (10/300 GL, GE Healthcare) equilibrated with 200 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified in one step by IMAC followed by size exclusion chromatography using ÅKTAxpress system (GE Healthcare) as previously described with some modifications (28) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 40-80 µg protein was used for western blotting (AmershamTM HybondTM - ECL nitrocellulose membranes, GE Healthcare, Chicago, IL) with a wet-transfer system (BioRad ...
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bioRxiv - Cell Biology 2020Quote: ... This mixture was then added to 30 μl of pre-equilibrated Protein G sepharose beads (GE Healthcare Life Science) and incubated ON under continuous agitation ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then further purified by size-exclusion chromatography on a 16/600 Superdex 200 column (GE Healthcare) equilibrated in either 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... after which protein-containing fractions were pooled and loaded onto pre-equilibrated Superdex 200 HiLoad 16/60 (GE Healthcare) for size exclusion chromatography and elution was performed with 25mM Phosphate buffer pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were separated from unreacted fluorophores by passing over a Superpose 6 Increase 10/300 GL column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then further purified by anion exchange chromatography using a 5 ml HiTrap Q HP column (GE Healthcare) across a gradient of 100 mM to 1M NaCl ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatants of the His6-tagged proteins obtained were incubated with 500μL Ni Sepharose (GE Healthcare 17-5318-06) and then washed twice with buffer A ...
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bioRxiv - Plant Biology 2020Quote: ... The secreted protein was purified from the supernatant by Ni2+ affinity chromatography on a HisTrap Excel column (GE healthcare), equilibrated in 50 mM KPi pH 7.6 ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were then washed five times for 5 min and proteins were detected with Amersham ECL (GE Healthcare). Images were taken using the iBright Imaging System (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... Purified analyte proteins were separated by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare) in HBS-EP just prior to use in SPR experiments to remove any protein aggregates that might influence kinetic measurements ...
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bioRxiv - Microbiology 2020Quote: ... The cleaved protein was concentrated and loaded onto a Superdex 75 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4 °C in 50 mmol L−1 Hepes at pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... The protein samples were further purified by gel filtration on a Superdex S75 HR 10/30 column (GE Healthcare). Samples were then concentrated to ~500 μM using 10 kDa cut-off centrifugal filter units (Amicon Ultra ...
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bioRxiv - Immunology 2021Quote: ... IgGs were purified from ~1 ml of plasma with equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted from the resin with 0.1 M glycine at pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...