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Citations for GE Life Sciences :
3501 - 3550 of 5100 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Signals were developed with horseradish peroxidase-conjugated secondary antibodies (GE Healthcare Bio-sciences) or peroxidase-conjugated primary antibodies using Western Lightning Plus-ECL (PerkinElmer ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibody binding was detected by chemiluminescence using the ECL detection system (GE Healthcare) or ECL Plus (for NICD ...
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bioRxiv - Immunology 2024Quote: ... wells were washed and incubated with horseradish peroxidase (HRP)-conjugated antibodies (GE Healthcare) for 3 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were incubated with HRP-conjugated anti-mouse secondary antibodies (Ge Healthcare, USA) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The secondary antibodies used were horseradish peroxidase-conjugated anti-mouse (NA931; GE Healthcare) and anti-goat (ab6741 ...
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bioRxiv - Biophysics 2021Quote: ... 500 µL sample was injected and run with a flow rate of 0.5 mL/min on an ÄKTA Pure protein purification system (GE Healthcare). Control samples containing ThT alone at the lowest (0.4 M urea ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were finally purified using size exclusion chromatography using a Superdex 200 column (10/300 GL, GE Healthcare, Germany) equilibrated with PBS buffer ...
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bioRxiv - Cell Biology 2020Quote: Fresh embryo protein extracts (4 mg in 2.5 ml RIPA buffer) was passed through PD-10 desalting column (GE Healthcare) to remove excess free biotin using the gravity protocol according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was concentrated using Amicon-3.5K filters and subjected to size exclusion chromatography on a Superdex 200 column (GE Healthcare). The concentration of eluted proteins was measured at 280 nm using a Nanodrop spectrophotometer and the proteins were verified by Coomassie Blue staining and western blotting ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was precleaned with 40 μL of 50% slurry of protein A Sepharose beads (GE Healthcare, Chicago, IL, USA) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The fractions containing the protein were concentrated and gel filtered on a Superdex 75 increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected by staining with Coomassie Brilliant Blue R250 or by transferring to a PVDF membrane (GE Life Sciences), and staining with Ponceau S dye or detection by immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions with >95% pure protein were pooled and re-purified from HiTrap Heparin HP affinity columns (GE Healthcare Life sciences) using a linear gradient of 0 – 300 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: Cells lysates were prepared using 1 % triton lysis buffer and precleared with prewashed Protein G Sepharose (PGS) beads (GE Healthcare) for 30 min on a shaker at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Fc mutations to prevent dimerization were introduced into the Fc sequence.61 Proteins were purified using MabSelect resin (GE Healthcare). Proteins were biotinylated using BirA enzyme (BPS Biosciences ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion EtAHS A protein was purified from cell lysates with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich ...
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bioRxiv - Physiology 2022Quote: ... Supernatants were collected after centrifugation at 13,200 rpm for 15 min and precleaned for 1 h with 50% protein G-Sepharose (GE Healthcare), followed by incubation with an indicated antibody at 4 °C Upon addition of 100 μl of 50% protein G-Sepharose ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was then loaded onto a transport buffer equilibrated Superdex 75 gel filtration column (GE Healthcare, #28-9893-33) and fractions containing the protein of interest were pooled ...
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bioRxiv - Molecular Biology 2020Quote: ... For each IP 100µL chromatin was pre-cleared with BSA blocked protein-G sepharose beads (GE Healthcare, Chicago, IL, USA) and incubated for 16h at 4°C with 2µL α-H3K27me3 antibody (#07-449 ...
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bioRxiv - Molecular Biology 2021Quote: Released protein was further concentrated to 0.5mL and then loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... Exposure of the resultant protein bands was performed with an ImageQuant LAS 4000 Luminescent Image Analyzer (GE Healthcare; Chicago, IL).
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bioRxiv - Microbiology 2022Quote: ... Cells were separated from supernatant by centrifugation and supernatant containing the secreted proteins was loaded onto a NiFF (GE Healthcare) column as described above.
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bioRxiv - Microbiology 2019Quote: ... Strep-KaiC36803 and Strep-KaiC7942 proteins were applied on a Sephacryl S300 HR HiPrep 16/60 Sephacryl column (GE Healthcare) and separated in 20 or 50 mM Tris/HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were cleared by centrifugation (20,000 rpm, 60 min) and proteins were purified from the supernatant using 7mL of Ni-NTA resin (GE Healthcare). The protein was eluted from the column with nickel-binding buffer containing 500mM imidazole.
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bioRxiv - Biochemistry 2019Quote: ... to 3 ml and the protein further purified by size exclusion chromatography (Hiload 16/600 Superdex 200 pg, GE healthcare) equilibrated with SEC buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then run over a size exclusion column (GE Healthcare HiPrep™ 26/60 Sephacryl S-300 HR) in sizing buffer (50mM Tris-HCl pH=8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... Protein-containing fractions were concentrated and further purified on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... Fluorescence profiles and peak volumes of labeled proteins were obtained using ImageQuant TL v2005 (GE Healthcare Life Sciences, Piscataway, NJ). For calculation of AA74-1 IC50 values (Fig ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was purified by size exclusion chromatography over a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare) equilibrated and run in Sizing Buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... the protein of interest was enriched via metal ion affinity chromatography (Ni-NTA) resin (IMAC, 5ml HiTrap Chelating, GE Healthcare). After 10 column washes with (20 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: A volume of 25-30 µL serum was spotted onto a Whatman® Protein Saver™ 903 Card (GE Healthcare), air-dried at room temperature for approximately 2 hours and stored at 4 °C until further use ...
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bioRxiv - Cell Biology 2019Quote: ... Processed protein was concentrated and further purified on a HiLoad Superdex 200 16/60 size exclusion chromatography column (GE Healthcare) equilibrated in 20 mM Tris HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were run on an 8% SDS-PAGE gel and the protein was then transblotted onto a 0.2 µm nitrocellulose membrane (GE Healthcare), followed by blocking for 3 hours in KPL block (SeraCare ...
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bioRxiv - Microbiology 2019Quote: ... 2.5 ml of fractions containing the highest protein content were pooled and the imidazole was removed by running the 2.5 ml protein suspension on PD-10 size-exclusion columns (GE Healthcare) followed by cold 3.5 ml of Buffer A to elute proteins ...
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Structural determinants of protocadherin-15 elasticity and function in inner-ear mechanotransductionbioRxiv - Biophysics 2019Quote: ... Refolded protein was concentrated (Vivaspin or Amicon 10 kDa) and further purified on Superdex S75 or S200 columns (GE Healthcare) in SEC buffer as indicated in Table S3 ...
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bioRxiv - Cell Biology 2019Quote: ... 5-10 μg proteins were resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). To visualize MLKL oligomers ...
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bioRxiv - Cell Biology 2019Quote: ... The lysates were clarified by centrifugation at 12,000 × g for 15 min and GST-Rab proteins were applied at saturating levels to glutathione Sepharose beads (GE Healthcare) for 30 min at 4°C and then washed extensively to remove unbound material ...
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bioRxiv - Immunology 2021Quote: ... TCR and pMHC proteins were dialyzed and further purified via anion exchange chromatography (HiTrap Q HP; Mono Q; GE Healthcare) and size-exclusion (Superdex 200 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein signal was detected by Clarity™ western ECL substrate and visualized by LAS680 imager (GE Life Sciences, Pittsburgh, USA). Images were analyzed by ImageJ (NIH ...
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bioRxiv - Biochemistry 2021Quote: ... The complex was separated from free D3_Sof protein by size-exclusion chromatography using “Superdex 75 10/300 GL” column (GE Healthcare) at 0.4 ml/min flow rate using running buffer containing 25 mM Tris-HCl (pH 7.6 at 25 ° C) ...
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bioRxiv - Biophysics 2021Quote: ... The dye-labeled protein was purified using a PD-10 column containing Sephadex G-25 Medium (GE Healthcare Life Sciences) and then centrifuged at 50000 rpm for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... The suspension of solubilized protein was ultracentrifuged at 100,000 × g for 1h before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with buffer containing 20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2021Quote: ... and protein further purified by size-exclusion chromatography using a HiLoad 16/600 Superdex 75 pg column (GE Life Sciences), 1.0 mL/min in Na2HPO4 pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... the bound fusion protein was eluted with 20– 200 mM imidazole gradient using a FPLC purifier system (ÄKTA; GE Healthcare). Peak fractions were pooled and digested with SENP2 protease (at final 0.01 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was further purified over a gel filtration column (HiPrep 16/60 Sephacryl S-100 High Resolution, GE Healthcare) equilibrated with AUC buffer (50 mM NaH2PO4 pH 7.0 ...
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bioRxiv - Biochemistry 2020Quote: ... 100-400 mM NaCl (depending on the protein) with either PD-10 or HiPrep 26/10 desalting columns (GE Healthcare). SGTA ...
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bioRxiv - Biochemistry 2020Quote: Proteins were transferred from the SDS-PAGE gel to a 0.2 μm Amersham Protran Nitrocellulose blotting membrane (GE life sciences) using a BioRad Trans-blot cell gel tank filled with transfer buffer (192 mM glycine ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare). The individual subunit homo-oligomeric rings or micro-complexes exist around 11–14 ml fraction from the size exclusion column.
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bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant containing the protein was loaded on a 5 mL HiTrap Ni-chelating column (GE Healthcare, Piscataway, NJ), washed ...
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bioRxiv - Biochemistry 2021Quote: ... The excess Cu(II) was eliminated by passing the protein through a PD MidiTrap G-25 desalting column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 and 150 mM NaCl.