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Citations for GE Life Sciences :
3551 - 3600 of 5100 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Supernant containing soluble proteins was filtered using a 0.45 μm device and applied to a HisTrap HP affinity column (GE Healthcare) equilibrated in buffer A (40 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... The complex protein was further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were expressed in Rosetta 2 bacteria and purified by affinity chromatography using a HiTrap™ chelating column (GE Healthcare) according to the manufacturer’s instructions in 50 mM Tris at pH 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... The cleared lysates containing the protein of interest were incubated 4h at 4°C with 1.5 μM of the indicated GST-fused proteins and 30 μl of glutathione-sepharose CL-4B beads (GE Healthcare). After three washes in lysis buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... and 20-50 μg of protein lysates were separated by SDS-PAGE and then transferred onto PVDF membranes (GE Healthcare). Membranes were saturated in Tris Buffer Saline/0.1%Tween (TBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... The protein samples were resolved on SDS-PAGE gels and then transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... ACE2-Fc proteins were then purified by size-exclusion chromatography (SEC) using Superdex 200 Increase column (GE healthcare, Chicago, IL) and stored at −80°C until used in experiments.
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bioRxiv - Biochemistry 2020Quote: ... purified proteins were mixed in a stoichiometric ratio and loaded on a SD200 Increase 10/300 GL column (GE Healthcare) equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: Protein samples were eluted from a Superdex 200 Increase 10/300 GL column using an ÄKTA Pure FPLC (GE Healthcare) at a flow rate of 0.7 mL/min ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were applied to a size-exclusion column (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) with the buffer containing 50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2020Quote: ... The blot was washed again and proteins were detected using the Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Final purification of GST-CsrA protein was conducted via size exclusion chromatography on a Superdex 75 16/60 (GE Healthcare) column using CsrA gel filtration buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... the proteins were further purified via size exclusion chromatography using a Superdex™ 200 Increase 10/300 GL (GE Healthcare) with 50 mM Tris/HCl buffer containing 100 mM KCl and 0.5 mM EDTA.
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bioRxiv - Microbiology 2020Quote: ... Eluted sample was further purified with fast protein liquid chromatography (FPLC) and gel filtration on a Superdex200 column (GE Healthcare) in sizing buffer (30 mM Tris·HCl ...
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bioRxiv - Biophysics 2021Quote: ... To remove aggregated protein the protein solution was filtered through a 0.2 µm filter and loaded on a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer for size-exclusion chromatography ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions containing desired proteins were pooled and further purified by SEC (HiLoad 16/60 Superdex 200 column, GE Healthcare) using sodium phosphate buffer A with pH 7.5 as eluent ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin was then precleared overnight at 4°C with vertical rotation at 15rpm (a total of 5 reactions per condition in 15ml tubes) with 500µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) beads and then used for immunoprecipitation.
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bioRxiv - Molecular Biology 2021Quote: ... and immunoprecipitated again in a single reaction with 100µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) pre-conjugated with histone H3 and histone H2B antibodies in order to specifically enrich for chromatin-associated dCas9 complexes and eliminate from the purification unspecific non-chromatin associated dCas9 complexes ...
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Fh15 polyclonal IgG was purified by affinity chromatography using 5/5/HiTrap Protein-A HP (GE Healthcare, Piscataway, NJ). The resulting IgG was conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Plant Biology 2021Quote: ... Untagged recombinant proteins were then concentrated and further purified by size-exclusion chromatography (Superdex 200 Increase10/300 GL; GE Healthcare) in buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Plant Biology 2021Quote: ... The proteins were then purified by affinity chromatography using Ni-NTA agarose resin and Glutathione Sepharose 4B resin (GE Healthcare), respectively.
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bioRxiv - Molecular Biology 2021Quote: ... Trimer-containing fractions were pooled and passed over a pre-equilibrated MAbSelect SuRe Protein A (ProA) affinity column (GE Healthcare) to remove leached residual PGT145-affinity ligand from the previous column ...
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bioRxiv - Microbiology 2020Quote: ... Antibody was expressed using ExpiCHO expression system (LifeTechnologies) according to the manufacturer’s protocol and purified using a Protein A MabSelect SuRE column (GE Healthcare). The wash buffer contained 20mM Tris & 150mM NaCl buffered to pH 8.6 and the elution was done using 0.1 M Citric acid pH 2.5 ...
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bioRxiv - Cell Biology 2021Quote: ... followed by transfer of proteins from gel to polyvinylidene fluoride (PVDF) membrane (Amersham Hybond P 0.45, GE Healthcare Life Sciences) and sequential use of primary and secondary antibodies that are listed in Table 3 ...
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bioRxiv - Cell Biology 2020Quote: ... The protein purification was done through affinity chromatography on a Ni-column (HiTrap chelating HP column 1 ml, GE HealthCare). The column was loaded with a filtered solution of 100 mM nickel chloride ...
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bioRxiv - Biophysics 2021Quote: ... The protein eluate was concentrated and further purified by sizeexclusion chromatography on a Superdex200 10/300 GL column (GE Healthcare) in Buffer B with 0.06% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... The protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 75 pg column (GE Healthcare) in HPLC buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were further purified by size exclusion chromatography (SEC) using Superdex200 16/60 HiLoad gel filtration column (GE Healthcare). For CLGltPh ...
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bioRxiv - Cell Biology 2020Quote: ... Protein signal was detected by Clarity™ western ECL substrate and visualized by LAS680 imager (GE Life Sciences, Pittsburgh, USA). Images were analyzed by ImageJ (NIH ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were electroblotted to 0.45μm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010; or Amersham Hybond, GE Healthcare Life Science, 10600023), and immunodetected using primary antibodies directed against phospho-Ser935 LRRK2 rabbit monoclonal (1/1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids encoding for GST fusion proteins of Cobl-like1-411 were generated by subcloning into pGEX-4T-2 (GE Healthcare). A plasmid encoding for TrxHis-Cobl-like1-411 was generated by PCR and subcloning into pET-32 (Novagen ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated on 10% Tris-Tricine gels and imaged using a Typhoon 9410 Molecular Imager (GE Healthcare Life Sciences).
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bioRxiv - Immunology 2022Quote: ... The protein solubilized in the previous step were subjected to a Ni HP-5 column (5 mL, GE Healthcare, USA), previously equilibrated with 5 CV of Buffer C (buffer C (50 mM sodium phosphate pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... the affinity-purified Pvs25 protein was applied to hydrophobic interaction chromatography (HIC) (HiTrap Phenyl HP; 5 mL resin, GE Healthcare) In detail ...
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bioRxiv - Neuroscience 2022Quote: ... We performed size exclusion chromatography on the eluted proteins in a Superdex 200 Increase 10/300 GL column (GE Healthcare) using an Akta Ettan fast protein liquid chromatography (FPLC ...
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bioRxiv - Neuroscience 2022Quote: ... we performed size exclusion chromatography on the concentrated proteins in a Superdex 200 Increase 10/300 GL column (GE Healthcare) using an Akta Ettan fast protein liquid chromatography (FPLC ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins were separated using standard SDS-PAGE electrophoresis techniques and transferred onto 0.45 μm nitrocellulose membranes (GE Healthcare, Amersham, U.K.) where non-specific binding was blocked by 5% (w:v ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by size exclusion chromatography on HiLoad 26/600 Superdex 200 prep grade column (GE Healthcare) equilibrated with the working buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Microbiology 2022Quote: ... and passed through a 0.45-μm filter prior to protein purification via affinity chromatography using an AKTA Start FPLC system (GE Healthcare). The lysate was loaded into a HisTrap HP 5 mL column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... TEV protease was added during dialysis to remove the N-terminal His6-tag.The protein was retrieved from dialysis and loaded onto a 1ml HiTrap SP HP IEX column (GE Healthcare) and eluted by gradient introduction of high-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Biophysics 2022Quote: ... recombinant proteins were purified twice by binding to and eluting from a Ni Sepharose 6 Fast Flow column (GE Healthcare) and a HiTrap Heparin HP column (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... Protein eluted from the affinity columns was further purified by size-exclusion chromatography using a Superdex 75 column (GE healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2022Quote: ... Rac1 protein went through a nickel affinity column followed by a Resource Q column and finally Superdex 75 (GE Healthcare) before concentration to 25mg/ml ...
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bioRxiv - Microbiology 2020Quote: ... Antibody supernatants were harvested seven days following transfection and purified using protein G affinity chromatography following the manufacturer’s protocol (GE healthcare). In order to validate the functional relevance of Arg100 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins (500 µl) were loaded on Superdex 75 10/300 GL or Superdex 200 10/300 GL columns (GE Healthcare) and eluted with buffer 20 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... CM were collected after 48h of transfection and filtered through 0.45 μm syringe filters and used for protein purification via ÄKTA pure 25 FPLC (GE Healthcare) equipped with 1-ml HisTrap affinity column (HisTrap HP ...