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Citations for GE Life Sciences :
3301 - 3350 of 4765 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The cleaved dCas9 protein was further purified with a 5 ml SP Sepharose HiTrap column (GE Life Sciences) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... Fab and partially cleaved IgG were applied on 1-mL HiTrap Protein L column (GE Healthcare Life Science). After loading ...
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bioRxiv - Immunology 2021Quote: ... The protein was further purified by size exclusion chromatography using Superdex 200 10/300 GL column (GE Healthcare) equilibrated with 20 mM HEPES at pH 7.4 ...
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bioRxiv - Genomics 2020Quote: ... The signal of resolved protein was visualized by Amersham enhanced chemiluminescence (ECL) Western Blotting Analysis System (GE Healthcare) using Amersham Hyperfilm ECL (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
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bioRxiv - Molecular Biology 2021Quote: ... the immobilized proteins were eluted and further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were assessed by SDS–PAGE followed by Coomassie blue staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Msh5 ChIP was performed using polyclonal Msh5 antibody (generated in rabbit) and Protein A Sepharose beads (GE Healthcare) on synchronized meiotic cultures as described in Krishnaprasad et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The concentrated proteins were then subjected to size-exclusion chromatography using a Superdex-75 GL column (GE healthcare pre-equilibrated with 25 mM Tris-HCl 6.8 ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants containing the protein extracts were then applied onto a HisTrap FF 5 ml column (GE Healthcare) loaded with nickel ions using an ÄKTA Start liquid chromatography machine (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted out and further purified by a mono Q 5/5 column (GE Healthcare) and a Superose S6 Increase 5/150 GL column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... the proteins were diluted to contain 25 mM NaCl and further purified on Resource Q column (GE Healthcare). Size exclusion chromatography on a HiLoad Superdex 75 16/600 column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... fritted deep-well plates were used containing 25 μL of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Microbiology 2020Quote: ... The protein was repurified on Co-NTA agarose followed by gel filtration chromatography on Superdex 200 (GE Healthcare) in buffer A to remove the protease ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples (20 µg) were separated on 15% SDS-PAGE and transferred to Protran nitrocellulose membranes (GE Healthcare). Membranes were blocked overnight at 4°C with 5% milk in TBS-T (20 mM Tris pH 7.6 ...
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bioRxiv - Biophysics 2020Quote: ... The eluted protein sample was concentrated and applied to gel filtration (Superose 6 10/300 GL, GE Healthcare) in running buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The desalted protein containing fractions were pooled and loaded on a Q-Sepharose XK16/32 column (GE Healthcare) equilibrated with 25mM MES pH 5.5 as running buffer ...
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bioRxiv - Biochemistry 2019Quote: ... cleaved proteins were concentrated and finally purified by gel size exclusion chromatography (Superdex G75 26/60, GE Healthcare) in buffer C.
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bioRxiv - Immunology 2019Quote: ... The eluted protein was concentrated and loaded on a Superdex 200 16/60 column (GE Healthcare, Chicago, IL) pre-equilibrated with PBS ...
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bioRxiv - Cell Biology 2019Quote: GST tag was removed from GST-Ana2 by incubating the resin-bound protein with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amount of 5-10µg proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). Membranes were revealed using a chemiluminescent HRP substrate (Millipore ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was further purified by size exclusion chromatography (SEC) (HiLoad 16/600 Superdex 200 pg, GE Healthcare) in 50 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... Protein containing fractions were loaded on SEC Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated with SEC buffer (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... and O-glycosylated proteins isolated by affinity chromatography using Concavaline A columns (HiTrap Con A 4B, GE Healthcare) in AKTA FPLC (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare; Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” (Perkin Elmer ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were visualized using the enhanced chemiluminescence (ECL) system (ECL™ Prime Western Blotting System, GE Healthcare Bioscience).
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bioRxiv - Microbiology 2019Quote: ... Anti-CFP polyclonal antibodies were purified by affinity chromatography using a HiTrap Protein A HP column (GE Healthcare) according to manufacturer instructions.
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bioRxiv - Microbiology 2019Quote: ... Membranes were solubilized in 1% (w/v) LMNG and proteins were purified using a StrepTrap column (GE Healthcare). The resin was washed in TBS containing 0.01% (w/v ...
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bioRxiv - Cell Biology 2019Quote: Protein purification was performed by immobilized metal-affinity chromatography (IMAC) on HisTrap HP Ni2+ Sepharose columns (GE Healthcare) at 4°C according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... fritted deep-well plates were used containing 25 μl of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Cell Biology 2020Quote: ... The processed proteins were concentrated and further purified by size exclusion chromatography (Superdex-75 16/60, GE Healthcare) in running buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... Supernatants were collected by centrifugation and purified using a 1 ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5 ml kappa-select column (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... The fractions containing protein were further using SEC column HiLoad 16/60 Superdex 75 prep grade (GE Healthcare) previously equilibrated with 50 mM KH2PO4 ...
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bioRxiv - Bioengineering 2021Quote: ... The protein was purified through nickel-agarose affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare). The purified protein was concentrated to 30 mg/mL in a buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Bioengineering 2021Quote: The purified protein was desalted in deionized Milli-Q water using a PD-10 column from GE healthcare and concentrated fivefold using a Thermo scientific three kDa MWCO protein concentrator ...
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bioRxiv - Biochemistry 2021Quote: ... the intact protein was further purified by cation exchange chromatography using a HiTrap SP HP column (GE Healthcare) and eluted by the 50– 500 mM NaCl gradient in 10 mM HEPES–KOH pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The α-domains of the proteins were purified to homogeneity by size exclusion chromatography (Superdex 75 GE Healthcare) equilibrated in 25 mM HEPES pH 7 ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel filtration with a Superose 6 10/300 GL column (GE Healthcare). hPCNA was expressed and purified as described in (Duffy et al ...
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bioRxiv - Biochemistry 2020Quote: All of the proteins characterized here eluted as homodimers as determined by a calibrated Superdex 75 (GE Healthcare) gel filtration chromatography column (25 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: Protein (450 μg/100 μL) was loaded on a Superdex™ 200 HR 10/30 column (GE Healthcare) equilibrated with PBS (140 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The tag-free protein was further purified through ion-exchange chromatography on a Heparin HP column (GE Healthcare) and size exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by gel-filtration chromatography on a Superdex 200 16/60 column (GE Healthcare) in Cas9 storage buffer (20 mM Tris-Cl ...
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bioRxiv - Cell Biology 2020Quote: ... the labeled protein was separated from the solution by size exclusion chromatography using PD MiniTrap G25 (GE Healthcare). PLCγ1 nSH2-cSH2-SH3 and truncations were labeled with CoA 647 (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was subsequently loaded into an ion exchange column (HiTrap® SP FF GE Healthcare Life Sciences), pre-equilibrated in the SP buffer A to remove 6His-ZB tag and uncleaved protein ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified from conditioned medium by cation exchange chromatography on a SP sepharose column (GE healthcare) in 50 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins separated by SDS-PAGE were detected following the manufacturer’s guidelines (GE Healthcare ECL Western Blotting detection system) and the images collected using a Chemidoc imaging system (BioRad Laboratories).
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further purified on a HiLoad 16/600 Superdex 75pg or 200pg gel-filtration columns (GE Healthcare). To obtain stoichiometric MLL1RWSAD complex ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 cells as described previously.13 The protein was purified using glutathione-Sepharose 4B beads (GE Healthcare) concentrated and flash-frozen in GST buffer [25 mM Tris (pH 7.5) ...