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Citations for GE Life Sciences :
3101 - 3150 of 4765 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Typically 100 µg of protein samples were injected into Superdex 200 10/300 increase column (GE Healthcare) by a Shimadzu autosampler coupled HPLC machine ...
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bioRxiv - Cell Biology 2021Quote: ... between 600-900 μg of each CLASP1 protein was injected into a Superose 6 column (GE Healthcare) in a buffer containing 50 mM phosphate pH 7.6 ...
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bioRxiv - Cell Biology 2021Quote: ... The next morning the cleaved protein was loaded on a 5 ml HiTrap HeparinHP column (GE Healthcare). The protein was eluted over a gradient from 100 mM to 2M KCl ...
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bioRxiv - Biophysics 2020Quote: ... the protein solution was fractionated using a DEAE Sepharose Fast Flow anion exchange chromatography resin (GE healthcare) eluting with a gradient from 0 to 1 M NaCl and further fractionated by size-exclusion chromatography using a HiPrep™ 26/60 Sephacryl® S-500 HR column ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 100 V for 90 min on ice in transfer buffer (48 mM Tris–HCl and 39 mM glycine supplemented with 20% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... The immuno-complexes were collected with 50 μl of protein A sepharose (17-0780-01, GE Healthcare) at 4 °C for two hours with rotation ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoreactive protein bands were visualized via enhanced chemiluminescence and scanned with a LAS-4000 system (GE Healthcare).
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bioRxiv - Evolutionary Biology 2022Quote: ... The protein sample was further purified using a Superdex200 26/600 size-exclusion chromatography column (GE Healthcare), and fractions were collected and concentrated to 1.8 mg/ml in 10 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated and further purified on a Superdex 75 16/600 GL column (GE Healthcare), in a size exclusion buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: Equal amounts of protein were resolved on 10% SDS-PAGE and blotted onto nitrocellulose membranes (GE Healthcare). Immunoblots were blocked with 5% fat-free milk in PBS containing 0.1% Tween-20 for 1h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The tagless protein was further purified by anion exchange chromatography (Mono Q 10/100 GL, GE Healthcare) in 25 mM potassium phosphate (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: Samples were precleared by adding 60 μl of 20% Protein A Sepharose™ CL-4B (GE Healthcare) in IP Buffer (15 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... the buffer exchanged protein was concentrated and injected on a SD200 10/300 increase column (GE Healthcare) equilibrated with 25 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Anti-TNFα IgG1 was purified on Protein A HiTrapHP columns (GE Healthcare Life Sciences, Little Chalfront, UK) using Akta-prime plus (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... partition coefficients of standard proteins from the high-molecular-weight gel filtration calibration kit (GE Healthcare #28403842) were plotted against Log(MW ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further purified using size exclusion chromatography (Superdex 200 Increase 10/300 GL; GE Healthcare) to remove nonspecific proteins ...
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bioRxiv - Biochemistry 2022Quote: ... Collected fractions containing membrane proteins were loaded onto a Superose 6 increase 10/300 column (GE Healthcare) and eluted with 30 mL of NCMN Buffer E ...
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bioRxiv - Biophysics 2022Quote: ... the modified proteins were purified and separated from unbound dye through a gel filtration column (GE Healthcare illustra NAP Columns from Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further purified by size exclusion chromatography using Superdex 200 16/60 column (GE Healthcare) equilibrated in buffer containing 25mM Tris-HCl pH8.0 ...
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bioRxiv - Cell Biology 2022Quote: Samples were separated on SDS-polyacrylamide gel and proteins were transferred onto a nitrocellulose membrane (GE healthcare) using a wet transfer system (BioRad) ...
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bioRxiv - Immunology 2020Quote: ... the supernatants with the secretion of antibodies were collected and captured by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and dialyzed into phosphate-buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins were further purified by size exclusion chromatography on a Superdex200 16/60 column (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... Rhesus sera or plasma antibodies were first purified by affinity chromatography using Protein A Sepharose (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The approximate molecular weights of the loaded proteins were determined by molecular weight calibration kits (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... the protein was concentrated and further purified by gel filtration using a superpose 6 increase (GE healthcare) in TBS containing 0.01% LMNG ...
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bioRxiv - Bioengineering 2021Quote: ... The protein was further purified by size-exclusion chromatography using a HiLoad Superdex 200PG column (GE Healthcare) with PBS as an eluent ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were purified by immobilized metal-affinity chromatography (IMAC) using pre-packed nickel Sepharose columns (GE Healthcare), or in batch mode on TALON® beads (Takara Bio ...
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bioRxiv - Immunology 2020Quote: ... and the protein was separated on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare) equilibrated with TBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were resolved by 10% SDS PAGE and transferred on a nitrocellulose membrane (Protran BA83, GE Healthcare) using a semi-dry system (Bio-Rad Laboratories S.r.l.) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were either stained with Coomassie brilliant blue G-250 or transferred to nitrocellulose membranes (GE Healthcare). The membranes were blocked with 5% milk in 0.05% Tween TBS at room temperature for 1 h and then incubated at 4 °C overnight with a specific polyclonal serum produced by immunization of mice with RBD produced in HEK-293T cells ...
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bioRxiv - Biochemistry 2020Quote: ... the proteins were buffer exchanged into the appropriate buffer using an Illustra NAP-5 column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then loaded on a Superdex 200 10/300 GL Tricorn gel filtration column (GE Healthcare), and eluted with 1.5 column volumes of gel filtration buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... the eluted protein was loaded onto a size exclusion column (Superdex 200 16/600 column, GE Healthcare) and eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Concentrated protein was loaded onto a 16/600 Superdex 200 gel filtration column (GE Healthcare Piscataway, NJ). The buffer conditions were the same as in Ni2+-NTA affinity chromatography but with 20 mM NaCl and no imidazole ...
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bioRxiv - Microbiology 2022Quote: ... Periplasmic proteins were extracted and CusF was purified on a 5 ml StrepTrap HP column (GE healthcare) equilibrated with buffer A (10 mM NaPi ...
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bioRxiv - Microbiology 2022Quote: ... proteins were eluted and further purified by size-exclusion chromatography on Superdex 75 (GE Healthcare Life Sciences). Purified DksA proteins were aliquoted inside a BACTRON anaerobic chamber (Shel Lab ...
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bioRxiv - Biochemistry 2022Quote: ... thrombin or PreScission protease and proteins were further purified by ion exchange chromatography (Source 15Q, GE Healthcare) and gel filtration chromatography (Superdex 200 ...
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bioRxiv - Microbiology 2019Quote: ... Untagged Orn proteins were recovered in the flow-through of a HisTrap Ni-NTA column (GE Healthcare), separated from His6-SUMO ...
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bioRxiv - Pathology 2020Quote: ... The presence of P1 proteins was revealed using the ECL Plus Western Blotting Detection System (GE Healthcare).
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bioRxiv - Biophysics 2019Quote: ... the eluted protein was loaded onto a size exclusion column (Superdex 200 16/600 column, GE Healthcare) and eluted with 20mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... where the concentrated protein sample was applied onto a Superose 6 10/300 GL column (GE Healthcare) equilibrated with SEC buffer (25 mM Tris-HCl pH 8 ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were visualized using Amersham ECL Prime Western Blotting Detection System and Amersham Hyperfilm ECL (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... resin and excess biotin was separated from the labeled protein by Superdex75 gel filtration chromatography (GE Healthcare) in 20 mM HEPES buffer pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the holo-proteins were then loaded onto a Superdex 75 Increase 10/300 GL column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Cancer Biology 2019Quote: ... The proteins were then transferred to a 0.45-μm PVDF membrane (Amersham Hybond, GE Healthcare, München, Germany) which was blocked in 2% bovine serum albumin (Amresco ...
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bioRxiv - Molecular Biology 2019Quote: ... the eluted protein was loaded onto a size exclusion column (Superdex 200 16/600 column, GE Healthcare) and eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... a frozen protein aliquot was thawed and loaded onto a Superdex HR 75 10/300 (GE Healthcare) size exclusion column and eluted with 150 mM NaCl ...
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Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infectionbioRxiv - Cell Biology 2020Quote: ... proteins were further concentrated with filters and then purified by anion exchange chromatography on HitrapQ (GE Healthcare) and collected fractions were further loaded onto size exclusion column (Superdex 75 16/60 ...
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bioRxiv - Plant Biology 2019Quote: ... The expressed protein domains were purified via Ni2+ affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The eluted proteins were subjected to buffer change by PD10 desalting column into ITC-buffer (25 mM BisTris-HCl pH6.0 ...
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bioRxiv - Microbiology 2019Quote: ... Dual-labeled capsid proteins were separated using a PD Mini-Trap G-25 desalting column (GE healthcare) with imaging buffer (50 mM Tris ...