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Citations for GE Life Sciences :
3501 - 3550 of 4765 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 200 µL of protein for analytical runs) was injected into the Superdex 75 size exclusion column (GE Healthcare) (volume less than 900 µL) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified over a size exclusion chromatography column (Hiload 16/60 Superdex75 column, GE Healthcare) equilibrated with buffer containing 20 mM MES pH 6.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 10 mM glutathione and further purified on a Superdex 200 10/300 (GE Healthcare) column in 50 mM Hepes pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was then loaded onto a HiLoad Superdex 75 16/60 size exclusion chromatography column (GE Healthcare) that had been pre-equilibrated in Hepes 30 mM ...
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bioRxiv - Biophysics 2023Quote: ... Protein was purified using a 20 mL NiNTA column (Cytiva) attached to an Akta M FPLC (GE Healthcare) and with a 300 mM imidazole elution buffer ...
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bioRxiv - Microbiology 2023Quote: ... The immunocomplex was captured by using protein G Sepharose 4 fast flow beads (17-0618-01/ GE Healthcare). The immunoprecipitated complex was washed two times with IP lysis buffer and one time with mili-Q ...
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bioRxiv - Biochemistry 2023Quote: ... The GST fusion protein was purified by affinity chromatography on glutathione-Sepharose (GE Healthcare Life Sciences (now Cytiva)) in 300 mM NaCl ...
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Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulationbioRxiv - Biochemistry 2023Quote: ... The spin-labeled proteins were further purified by HiLoad 26/600 Superdex 200 pg column (GE life sciences) in a buffer of 150mM NaCl ...
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bioRxiv - Plant Biology 2023Quote: ... Protein containing fractions were pooled and buffer exchange was carried out using a PD-10 column (GE Healthcare) using TES-KCl buffer (25 mM TES/KOH pH 7.5 ...
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bioRxiv - Physiology 2022Quote: ... The specific proteins were detected with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000 dilution; CtNo_NA934, GE Healthcare, 1:5000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS/PAGE in 10% gel before wet transfer to 0.45μm nitrocellulose membranes (GE Healthcare) and blocked in 5% dry milk powder in TBS-T (100mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... Aurora A protein was thawed and buffer exchanged in the experimental buffer using NAP-5 Columns (GE Healthcare). Experiments typically involved titrating 25 µM of protein in the sample cell with 300 µM of compound in the syringe ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein eluate was then applied to an ion exchange chromatography column (2x 1ml HiTrap Q HP, GE Healthcare) equilibrated with QA buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to a nitrocellulose membrane with a 0.2 μm pore size (GE Healthcare Life Sciences, 10600001) by wet transfer at 100 V for 120 minutes ...
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bioRxiv - Plant Biology 2023Quote: Thylakoid proteins were separated by SDS-PAGE and then transferred to a polyvinylidene membrane (Hybond P, GE Healthcare) using a tank blot system (Perfect Blue Web M ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... then stored at −20°C before purification by fast protein liquid chromatography (FPLC) (AKTA Purifier; GE Healthcare, UK) using a pre-packed HiLoad Superdex 30 26/600 column equipped with a UV detector ...
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bioRxiv - Plant Biology 2024Quote: ... the proteins were subjected to size exclusion chromatography on a Superdex 200 10/300 increase column (GE Healthcare) and eluted using 150 mM Tris–HCl pH 8.8 containing 300 mM sodium chloride ...
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bioRxiv - Plant Biology 2024Quote: ... the eluted proteins were purified on a HiLoad16/60 Superdex 200 prep-grade gel filtration column (GE Healthcare) using a buffer containing 20 mM Tris (pH 7.5) ...
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bioRxiv - Immunology 2024Quote: ... GP38 proteins were further purified via SEC using a Superdex 200 Increase 10/300 GL (GE Healthcare Biosciences) in 2 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... The concentrated protein was injected into a Superdex 200 Increase 10/300 GL (GE Healthcare 28-9909-44) size exclusion column chromatography system (Waters) ...
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bioRxiv - Immunology 2024Quote: ... Proteins were separated on 12% SDS polyacrylamide gels and transferred to nitrocellulose membrane (Amersham Hybond-ECL, GE Healthcare). Membranes were blocked in 5% dried milk/TBS-T buffer and incubated over night with anti-mouse TREX1 antibody (clone C-11 ...
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bioRxiv - Biochemistry 2024Quote: ... and the flowthrough containing phosphorylated proteins was purified over Hiload 16/600 Superdex 75 pg column (GE Healthcare) preequilibrated with buffer containing 25 mM Tris (pH 7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein solutions was loaded on to Superose 6 Increase 10/300 GL column (GE Healthcare, Madison, WI) that was equilibrated with a buffer containing 20 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Biophysics 2024Quote: ... Protein was concentrated and inject into size-exclusion chromatography on a Superdex 16/60 75 column (GE Healthcare) pre-equilibrated in buffer 20 mM Bis-Tris pH 7.0 ...
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bioRxiv - Biochemistry 2024Quote: ... the protease was removed from (un)cleaved target protein using a HisTrap FF column (GE Healthcare Life Sciences). Remaining nucleic acids were removed using anion exchange chromatography (AIEX ...
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bioRxiv - Biochemistry 2024Quote: ... TEV protease and non-cleaved protein was removed by IMAC (HisTrap FF Crude column, 5 ml, GE Healthcare) with a linear gradient of imidazole (0-500 mM) ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... Fractions containing target protein were pooled and incubated over night at 4°C with PreScission protease (GE Healthcare) at a ratio of 1:100 (w/w ...
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bioRxiv - Biochemistry 2024Quote: ... Elution fractions containing the most protein were combined and loaded directly onto a size-exclusion column (GE Healthcare) pre-equilibrated with lysis buffer without prior concentration or dialysis of the protein ...
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bioRxiv - Biochemistry 2024Quote: Proteins were purified by immobilized metal-affinity chromatography (IMAC) on a HisTrap HP nickel-Sepharose column (GE Healthcare) at 4°C following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... thawed protein samples were purified by size-exclusion chromatography using a Superdex 200 (16/600) column (GE Healthcare) in equilibrated in a buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein bands were visualized and imaged by using an ImageQuant LAS 500 scanner (GE Healthcare, Chicago, IL, USA). After transfer ...
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bioRxiv - Microbiology 2024Quote: ... All protein lysates were separated on 10% polyacrylamide gels and blotted on polyvinyl difluoride membranes (PVDF, GE Healthcare). Blotted membranes were blocked with either 5% non-fat milk or 5% BSA ...
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bioRxiv - Microbiology 2020Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... Cleaved protein was filtered (0.45 µm) and further purified by size-exclusion chromatography (Superdex S200 26/60; GE Healthcare) in buffer containing 20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM DTT at pH 7.0) while incubating with PreScission protease (protease to protein ratio 1:2000) (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with corresponding antibody and chemiluminescence was visualized according to the manufacturer’s instructions (ECL Advanced; GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... and proteins were visualized using the with Image-Quant LAS 4000 biomolecular imager (GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Cell Biology 2020Quote: ... 35S-labeled proteins were separated by SDS-PAGE and detected with an Autoimaging analyzer (Typhoon FLA-9500; GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: The concentrated protein was then applied to a HiPrep 26/60 Sephacryl S-100 column (DEAE FF) (GE healthcare) which was equilibrated with the re-suspension buffer supplemented with 100 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) and the protein concentration was determined using a 2-D Quant kit (GE Healthcare, Piscataway NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The labelled protein was further purified by size-exclusion chromatography using Superdex 200 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES at pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein fraction eluted with 250 mM imidazole was purified further by MonoQ 5/50 GL column (GE Healthcare) at pH 8.0 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The cleaved protein was then concentrated to 2.0 ml final concentration and loaded on the S200 column (GE healthcare). S200 size exclusion chromatography has yielded a single peak around 230 KDa ...
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bioRxiv - Developmental Biology 2020Quote: ... The protein were electrophoresised for 1h at 150V and subsequently transferred onto MtOH activated polyvinylidene fluoride membranes (GE Healthcare). Equal loading was assessed using Ponceau red solution ...
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bioRxiv - Neuroscience 2021Quote: ... The labeled proteins were separated from the free dyes on a homemade Sephadex G50 resin (GE Healthcare, Piscataway, NJ) column in TBS buffer.
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bioRxiv - Molecular Biology 2020Quote: ... proteins were separated by SDS-PAGE and transferred to AmershamTM ProtranTM 0.45 μm nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were probed with primary antibodies at dilutions recommended by the suppliers ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody-chromatin complexes were purified with Protein A conjugated to sepharose beads (GE Healthcare, 30 µL GE17-5280-01) for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The ion-exchange chromatography was performed for further protein purification with an anion-exchange column (Source 15Q, GE Healthcare). Then peak fractions were collected and stored at -80 °C before using.
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred from polyacrylamide to nitrocellulose membranes (AmershamTM ProtranTM Premium 0.45 µm NC, GE Healthcare Life sciences, 10600003) by electrotransfer (Bio-Rad laboratories Inc.) ...
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bioRxiv - Immunology 2019Quote: ... after which proteins were transferred to nitrocellulose membranes (Amersham Hybond ECL 0.45 µm Nitrocellulose Membrane, GE Healthcare Life Sciences) or PVDF membranes (Amersham Hybond P 0.45 µm PVDF Membrane ...