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Citations for GE Life Sciences :
3451 - 3500 of 4765 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification.
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bioRxiv - Immunology 2020Quote: ... we used fritted deep well plates containing 25 μL of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was further purified by gel filtration using a HiLoad Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... fritted deep-well plates were used containing 25 μl of settled protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Immunology 2021Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatant using a HiTrap protein A column (Cytiva, GE Healthcare). IgG3 antibodies were isolated using a HiTrap Protein G High Performance column (Cytiva ...
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bioRxiv - Microbiology 2021Quote: Proteins resolved by SDS PAGE were transferred to a nitrocellulose transfer membrane (GE Healthcare Amersham™ Protran™) at 80 V for 75 minutes or 15 V overnight at 4°C using a Bio-Rad wet transfer apparatus ...
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bioRxiv - Immunology 2021Quote: ... The protein was further purified on a Superdex 200 Increase 10/300 GL size exclusion column (GE Healthcare). SDS-PAGE was performed to determine the purity and size of the protein ...
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bioRxiv - Plant Biology 2020Quote: Thylakoid proteins were separated by SDS-PAGE and then transferred to a polyvinylidene membrane (Hybond P, GE Healthcare) using a tank blot system (Perfect Blue Web M ...
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bioRxiv - Cell Biology 2021Quote: ... protein extracts were subject to SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL). Primary antibodies used were anti-P2Y1 (Santa Cruz ...
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bioRxiv - Biochemistry 2022Quote: ... Salt concentration is adjusted and protein is loaded on a 10 ml HiTrap Heparin HP column (GE Healthcare) equilibrated in 20 mM HEPES- KOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... All target proteins were subjected to size exclusion chromatography using the HiLoad 16/600 Superdex 75 (GE Healthcare) equilibrated in 25 mM Tris pH8.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... After protein fractionation by SDS-page samples were transferred to a nitrocellulose membrane (cat#10600002 from GE healthcare) and incubated with primary antibody solutions overnight at 4 °C (p21 ...
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bioRxiv - Immunology 2022Quote: The total IgG was purified from murine serum using Protein G Sepharose 4 Fast Flow columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein was loaded and separated in SDS-page gels and then transferred to PVDF membrane (GE Healthcare). For blocking ...
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bioRxiv - Plant Biology 2022Quote: ... Protein was concentrated using a spin concentrator (Amicon, 10K MWCO) and injected onto Superdex 200 column (GE Healthcare) equilibrated in 25 mM HEPES (pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... protein samples were run on 12-15% polyacrylamide gels and transferred to nitrocellulose Hybond™-C (GE Healthcare) membranes via Bio-Rad Trans-blot Turbo transfer machine ...
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bioRxiv - Microbiology 2022Quote: ... the protein was concentrated and loaded onto a Superdex 75 16/60 size exclusion (SEC) column (GE Healthcare). The peak corresponding to the protein was concentrated to about 12 mg/ml and flash frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Microbiology 2022Quote: ... The supernatants containing antibodies were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... The protein solutions were further diluted with buffer B and eIF4A1 fractions subjected to a ResourceQ (GE Healthcare) anion exchange column ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted proteins were concentrated and applied onto a Superdex 75 HiLoad 26/60 size-exclusion column (GE Healthcare) pre-equilibrated with gel-filtration buffer (10 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then visualized by chemiluminescence reagent (Cyanagen(XLS3,0100),Thermo Fisher(34580)) using Amersham Imager 600 (GE Healthcare) and band densitometry was measured using FIJI software ...
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bioRxiv - Developmental Biology 2022Quote: ... Molecular weights were determined using pre-stained protein ladders (Thermo Fisher Scientific, catalog #26616; GE Healthcare, catalog #RPN800E). Protein abundance was detected on membranes using the SuperSignal West Femto enhanced chemiluminescence kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Immunoprecipitation was performed by incubating sheared chromatin with Protein G Sepharose™ 4 Fast Flow beads (GE Healthcare) and antibodies ...
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bioRxiv - Immunology 2022Quote: ... the supernatant was diluted to a total protein concentration of 100 μg/ml in binding buffer (GE Healthcare). Following purification ...
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bioRxiv - Neuroscience 2024Quote: ... The immuno-complexes were then incubated with 30 μl protein G sepharose beads (GE healthcare, 17-0618-01), which were pre-equilibrated with Lysis Buffer ...
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bioRxiv - Plant Biology 2024Quote: ... the proteins were subjected to size exclusion chromatography on a Superdex 200 10/300 increase column (GE Healthcare) and eluted using 150 mM Tris–HCl pH 8.8 containing 300 mM sodium chloride ...
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bioRxiv - Plant Biology 2024Quote: ... the eluted proteins were purified on a HiLoad16/60 Superdex 200 prep-grade gel filtration column (GE Healthcare) using a buffer containing 20 mM Tris (pH 7.5) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins were separated by 8-10% SDS-PAGE and transferred to a Hybond ECL nitrocellulose membrane (GE Healthcare) or Amersham Protran 0.45 μm NC nitrocellulose membranes (Carl Roth ...
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bioRxiv - Immunology 2024Quote: ... GP38 proteins were further purified via SEC using a Superdex 200 Increase 10/300 GL (GE Healthcare Biosciences) in 2 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 μl of a protein A/G sepharose mixture (GE Healthcare; #17-0780-01 and #17-0618-01), pre-equilibrated in ChIP dilution buffer was added to the lysates and incubation continued for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... fritted deep-well plates were used containing 25 μl of settled Protein G resin (GE Healthcare Life Sciences) per well ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was then incubated with 30 µl 50% protein A-Sepharose slurry (GE Healthcare, 17-0780-01) for 1 hr at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were visualized using the Amersham ECL Prime Western Blotting Detection System and Amersham Hyperfilm ECL (GE Healthcare). The following primary antibodies were used ...
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bioRxiv - Microbiology 2024Quote: Analytical size-exclusion chromatography was performed using an AKTA fast protein liquid chromatography (FPLC) system (Amersham GE Healthcare). The concentrated samples were loaded into a Superose12 HR 10/30 FPLC column (GE ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then incubated with washed and pre-blocked protein G Sepharose beads (50 µl, GE Healthcare) for 45 mins at 4°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... all proteins were further purified by gel filtration on a HiLoad 16/60 Superdex 75 column (GE Healthcare) in 40 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... and fermentation supernatants were purified by Protein-A affinity chromatography using MabSelect™ column (GE Healthcare, Princeton, NJ). The bispecific antibody was further polished by cation exchange chromatography using Capto MMC (GE Healthcare ...
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bioRxiv - Plant Biology 2023Quote: Thylakoid proteins were separated by SDS-PAGE and then transferred to a polyvinylidene membrane (Hybond P, GE Healthcare) using a tank blot system (Perfect Blue Web M ...
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bioRxiv - Biophysics 2023Quote: ... the protein was subjected to size-exclusion chromatography using sephacryl S-100 HR 16/60 column (GE HealthCare). The final purified protein was concentrated to 1 mM using Amicon (3 kDa cutoff ...
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bioRxiv - Biochemistry 2022Quote: ... The protein constructs were further purified by size exclusion chromatography using an ÄKTA Explorer FPLC system (GE Healthcare) loaded with two Superdex75 200 mL columns ...
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bioRxiv - Biochemistry 2023Quote: The protein was further purified by size exclusion chromatography (SEC) (Superdex 200 Increase 10/300 GL, GE Healthcare) in 20 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated with mouse monoclonal anti-MYC-antibody (Covance) and protein G-agarose beads (GE Healthcare). The precipitates were then washed with a lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated on 10 % or 14% SDS-PAGE and transferred to nitrocellulose membranes (#10600002, GE Healthcare Biosciences). The membranes were blocked with 1% BSA in TBS-Tween 20 (TBST ...
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bioRxiv - Biophysics 2023Quote: ... Protein fractions were diluted two-fold in buffer A and loaded onto a Source 15Q column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Microbiology 2023Quote: Sample protein content present on the SDS-PAGE was transferred onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare) for 90 min (25 V ...
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bioRxiv - Microbiology 2023Quote: ... proteins were separated by immobilized-metal affinity chromatography (IMAC) on a 5 mL HisTrap HP column (GE Healthcare), previously washed with ddH2O and equilibrated with buffer B (25 mM HEPES/NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... thawed protein samples were purified by size-exclusion chromatography using a Superdex 200 (16/600) column (GE Healthcare) in equilibrated in buffer 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2023Quote: ... The ErCas12a protein in clarified lysate was sequentially purified using immobilized metal affinity chromatography (HisTrap HP, GE Healthcare) and heparin chromatography (HiTrap Heparin HP ...
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bioRxiv - Biochemistry 2023Quote: ... 200 µL of protein for analytical runs) was injected into the Superdex 75 size exclusion column (GE Healthcare) (volume less than 900 µL) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified over a size exclusion chromatography column (Hiload 16/60 Superdex75 column, GE Healthcare) equilibrated with buffer containing 20 mM MES pH 6.5 ...