Labshake search
Citations for GE Life Sciences :
3401 - 3450 of 4765 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: ... the concentrated protein sample was applied onto a HiLoad Superdex 200 16/60 prep grade column (GE Healthcare) equilibrated with SEC buffer (25 mM CAPS pH 10 ...
-
bioRxiv - Microbiology 2019Quote: ... The elution fractions containing the purified protein were loaded onto a PD MidiTrap G-25 column (GE healthcare) equilibrated with 20 mM phosphate buffer (pH 7.4 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Protein samples were subjected to 10% SDS-PAGE and transferred to Hybond-ECL (GE Healthcare, Pittsburgh, PA, USA). The blotted membranes were blocked in 2% non-fat milk for 30 min and then incubated with primary antibodies (1:2,000 ...
-
bioRxiv - Biochemistry 2019Quote: ... The proteins were further purified by gel filtration through a Superdex 75 column (GE Healthcare, 29-1487-21) eluted with pH 7.4 PBS and 1 mM DTT or 1 mM TCEP ...
-
bioRxiv - Bioengineering 2019Quote: ... proteins in 2% SDS were bound to Paramagnetic beads (SeraMag Speed beads, GE Healthcare, CAT#45152105050250, CAT#651521050502) on filter plates (Multiscreen ...
-
bioRxiv - Biochemistry 2019Quote: ... The clarified protein solution was applied onto a 10/300 Tricorn column prepacked with Superdex 200 (GE Healthcare) and pre-equilibrated with buffer B containing 0.2% lauryl sarcosine ...
-
bioRxiv - Biochemistry 2019Quote: ... The eluted protein was further purified through size exclusion chromatography (SEC; Superose 6, 10/300 GL, GE Healthcare) in buffer containing 20 mM HEPES ...
-
bioRxiv - Biochemistry 2019Quote: ... mTLR9-ECD was purified by protein A-affinity chromatography with IgG Sepharose 6 Fast Flow resin (GE Healthcare), washed with PBS and eluted with 0.1 M glycine-HCl pH 3.5 ...
-
bioRxiv - Cell Biology 2019Quote: ... and the protein further purified by size exclusion chromatography using a Superdex-75 (26/600) column (GE Healthcare). The column was pre-equilibrated and run with (NMR-buffer ...
-
bioRxiv - Developmental Biology 2020Quote: ... Lysates were precleared using 20 μL protein G Sepharose 4 fast flow (1:1 in PBS, GE Healthcare) for 30 minutes at 4°C on a shaker ...
-
bioRxiv - Microbiology 2020Quote: ... the two proteins were mixed together in solution and purified using a Superdex200 gel filtration column (GE Healthcare). The complex was concentrated to 10 mg/ml in buffer 20 mM Tris pH 7.2 and 200 mM NaCl ...
-
bioRxiv - Molecular Biology 2019Quote: ... The proteins from the cleared cell lysate were captured in a HisTrap HP 5 ml column (GE Healthcare) in His buffers (20 mM MOPS ...
-
bioRxiv - Pathology 2020Quote: ... wt:wt) was determined by size exclusion chromatography in AKTA Primer Plus fast protein liquid chromatography system (GE Healthcare) with Superose™ 6 10/30 GL Column (GE Healthcare ...
-
bioRxiv - Immunology 2020Quote: ... run at a flow rate of 0.5-1 mL/min on Äkta Start protein purification system (GE Healthcare). Antibodies were eluted using 0.1M glycine pH 2 ...
-
bioRxiv - Biochemistry 2020Quote: ... This protein was applied to a 16/600 Superdex 75 preparative size exclusion column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A ...
-
bioRxiv - Biochemistry 2021Quote: ... Target protein was further purified by passage through a HiLoad 16/600 Superdex 75 pg (GE Healthcare, USA) in buffer E (20 mM Tris-HCl ...
-
bioRxiv - Immunology 2020Quote: ... The proteins were detected using the Amersham ECL Prime Western Blotting Detection Reagent (RPN2232, GE Healthcare Life Sciences) and visualized on the ECL Chemocam Imager (INTAS) ...
-
bioRxiv - Systems Biology 2021Quote: ... Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare), and purity was confirmed to be at least 90% by SDS polyacrylamide gel electrophoresis ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Concentrated protein was loaded onto a HiLoad 26/60 Superdex 200 prep grade column (GE Healthcare, Piscataway, NJ) that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Concentrated protein was loaded onto a HiLoad 26/60 Superdex 75 prep grade column (GE Healthcare, Piscataway, NJ) that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated and further purified using an S200 16/60 gel filtration chromatography column (GE Healthcare) previously equilibrated with buffer A ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protein bands were detected with the enhanced chemiluminescence method (FUJIFILM Wako) and chemiluminescence film (GE Healthcare, Buckinghamshire, UK), and target protein levels were analyzed using NIH ImageJ software.
-
bioRxiv - Microbiology 2020Quote: ... protein bands were visualized by chemiluminescence and the image was captured by ImageQuant LAS 4000 mini (GE Healthcare).
-
bioRxiv - Biophysics 2020Quote: ... Protein samples were resolved on the SDS-PAGE and transferred onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare). The membrane was blocked with 5% non-fat dry milk in tris-buffered saline ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Proteins of interest were visualized on a Bio-Rad ChemiDoc MP imager with Enhanced Chemiluminescence Plus (GE Healthcare) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher ...
-
bioRxiv - Immunology 2020Quote: Cell lysates (10μg protein) were resolved by electrophoresis and transferred to a polyvinylidene fluoride membrane (GE Healthcare, UK). Membranes were blocked for 1 hour in 5% skimmed milk/TBS/0.1% Tween-20 and incubated with primary antibodies (1:1000 dilution ...
-
bioRxiv - Microbiology 2020Quote: ... Protein was further purified by size exclusion over a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) in 100 mM NaCl ...
-
bioRxiv - Immunology 2020Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification ...
-
bioRxiv - Microbiology 2021Quote: ... The protein extracts were separated by SDS-PAGE and were subsequently transferred to Amersham Protran membranes (GE Healthcare) in a tankblot device (BioRad ...
-
bioRxiv - Molecular Biology 2019Quote: ... The absorbance of fluorescently labeled proteins was followed during chromatography using the AKTAmicro Monitor UV-900 (GE Healthcare) and by SDS-PAGE using a ChemiDoc MP system (Bio-Rad).
-
bioRxiv - Plant Biology 2019Quote: ... The protein was then further purified by further purified by gel filtration (Superose 6, 10/30; GE Healthcare). For cryo-EM investigation ...
-
bioRxiv - Molecular Biology 2019Quote: ... The concentrated protein sample was then applied to Superdex 200 Increase 10/300 GL Increase column (GE Healthcare) equilibrated with buffer containing 25 mM HEPES-HCl ...
-
bioRxiv - Cancer Biology 2019Quote: ... The resulting tag-cleaved proteins were purified by ion exchange chromatography with a HiTrap SP column (GE Healthcare) and gel filtration with the final buffer (25 mM Tris-HCl buffer at pH 7.0 ...
-
bioRxiv - Biochemistry 2019Quote: ... The protein was then purified by anion exchange chromatography on a MonoQ 5/50 GL column (GE Healthcare) using buffers AEX-A and AEX-B with a gradient of 7.5-50% B over 20 CV at a flow rate of 1 ml/min ...
-
bioRxiv - Cell Biology 2021Quote: ... 10 µg of proteins were resolved in Tris-acetate SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). For extracellular vesicle fractions ...
-
bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superose 6 Increase 3.2/300 column (GE Healthcare) equilibrated at 4°C in 40 mM HEPES pH 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superdex 200 Increase 3.2/300 column (GE Healthcare) equilibrated in 40 mM HEPES pH 7.4 (4 °C) ...
-
bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superose 6 Increase 3.2/300 column (GE Healthcare) equilibrated at 4°C in 40 mM HEPES pH 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: ... The excess dye was separated from the labelled protein by SEC (Superdex 75 10/300 GL, GE Healthcare) performed in SEC buffer D (20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2021Quote: ... His6-tag free Ape1 protein was then loaded onto a Superdex 200 10/300 GL column (GE Healthcare) in 20 mM Tris-HCl pH 7.0 ...
-
bioRxiv - Biochemistry 2021Quote: ... cells according to the high titer protocol provided and purified on a Protein A HiTrap column (GE Healthcare) with the ACTA Pure FPLC system (GE Healthcare) ...
-
bioRxiv - Biochemistry 2021Quote: ... the bromodomain was purified further using fast-protein liquid chromatography on an AKTA Purifier UPC 10 (GE Healthcare), equipped with a HiPrep 16/60 Sephacryl S-100 High Resolution column (GE Healthcare) ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare, USA) and purified using Assay Buffer or Assay Buffer without EDTA (for use in DNA cleavage assays) ...
-
bioRxiv - Biophysics 2021Quote: ... The eluted protein was further purified using a Superdex Increase 10/300 GL column (GE Healthcare, Chicago, IL) equilibrated with buffer containing 350 mM NaBr ...
-
bioRxiv - Cancer Biology 2021Quote: ... We subjected equal amounts of proteins to SDS-PAGE and transferred them to nitrocellulose membranes (GE Healthcare #10401197). Non-specific binding was blocked by incubation with TBST plus 5% non-fat milk ...
-
bioRxiv - Cell Biology 2020Quote: ... The protein was loaded on a HiPrep Q FF Anion Exchange Column (GE Healthcare Life Sciences, Marlborough, MA) and eluted by buffer with 300 mM NaCl ...
-
Conformational dynamics at microsecond timescale in the RNA-binding regions of dsRNA-binding domainsbioRxiv - Biophysics 2019Quote: SEC-MALS analysis of the purified proteins was performed on Superdex 75 10/300 GL column (GE Healthcare) using an Agilent HPLC system equipped with the 18-angle light scattering detector (Wyatt Dawn HELIOS II ...
-
bioRxiv - Microbiology 2021Quote: ... The protein complex was then purified by SEC using a Superdex 200 Increase 10/300 GL (GE Healthcare) column ...
-
bioRxiv - Cell Biology 2019Quote: ... for the detection of ubiquitinated protein aggregates or a 0.2 μm pore size cellulose acetate membrane (GE Healthcare) for the detection of huntingtin aggregates using a 96 well format Dot-Blot apparatus (Bio-Rad) ...
-
bioRxiv - Microbiology 2020Quote: ... All mAbs were purified from cell culture supernatant using a HiTrap protein G HP affinity column (GE Healthcare) and stored at −80 °C ...