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Citations for GE Life Sciences :
3301 - 3350 of 4095 citations for Cow Ataxin 10 ATXN10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The flow through was collected and further purified by size exclusion chromatography on the Superdex 200 Increase 10/300 GL (GE Healthcare). Peak proteins were pooled and verified by denaturing SDS PAGE ...
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bioRxiv - Neuroscience 2024Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 or Amicon 15 centrifugal concentrators (10 kDa, 5 kDa, or 3 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Genomics 2024Quote: ... extension products were separated by electrophoresis on a 10% denaturing PAGE gel and visualized by a Typhoon Phosphorimager (GE Healthcare, Inc).
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bioRxiv - Biophysics 2024Quote: ... The column was washed with 40 column volumes of the same buffer (without MTSL) and further eluted with 300 mM imidazole and immediately deslated using a PD-10 desalting column (GE Healthcare) into the same buffer ...
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bioRxiv - Biophysics 2024Quote: ... Collected nanobody fractions were then fractionated by size exclusion chromatography (SEC) using a Superdex 75 Increase 10/300 GL column (GE Healthcare) in HBS buffer.
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated using a 100 kDa filter to approximately 1 mL and further purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare) equilibrated with buffer containing 0.06% digitonin ...
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bioRxiv - Biochemistry 2024Quote: ... Concentrated eluates were then applied to a 24-mL Superdex S200 increase 10/300 GL size exclusion column (GE Life Sciences). Fractions containing Cdc13 (Fig ...
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bioRxiv - Molecular Biology 2024Quote: ... The membranes were washed with TBS-T three times for 10 minutes each and reacted with HRP-linked anti-rabbit or anti-mouse antibody (GE Healthcare) at room temperature for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: Gel filtration was performed using concentrated IMAC purified proteins of 500µl in volume using a Superdex 75 GL column and Superdex-200 10/300 GL column preparative grade size exclusion chromatography column (GE Healthcare). The column was pre-equilibrated with SEC buffer composed of 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: Size-exclusion chromatography (SEC) was employed as the final purification step using a Superdex 200 Column (Superdex 200 Increase 10/300 GL, GE Healthcare) pre-equilibrated with three column volumes of SEC buffer (Tris-NaCl) ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was concentrated to 100 µL using a 100 kDa MWCO concentrator and the concentrate was loaded onto a size-exclusion chromatography (SEC) column (Superose 6 Increase 10/30 GL, GE Healthcare), equilibrated in a buffer composed of 20 mM Tris-Cl (pH 8) ...
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Biophysics 2024Quote: ... monomeric and tetrameric protein were separated and collected by SEC using a Superdex 75 Increase 10/300 GL column (GE Healthcare) and then used directly in fluorescence experiments (SI Appendix ...
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bioRxiv - Biochemistry 2024Quote: ... Target proteins in the HiTrap SP FF column were eluted by a linear gradient of NaCl and further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare). The purified proteins were of high purity (>95 % ...
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bioRxiv - Immunology 2024Quote: ... Complexes were generated by incubating Fab and antigen overnight at a 50:1 ratio and purified through size exclusion chromatography with a Superdex 200 increase 10/300 column (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... and flow-through containing the Fabs was buffer exchanged to TBS using Vivaspin filters with a 10 kDa molecular weight cutoff (GE Healthcare).
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bioRxiv - Immunology 2024Quote: ... The S proteins were then separated by chromatography using resin attached with streptavidin and further purified by size exclusion chromatography using a Superose 6 10/300(GE Healthcare) in 20 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... or mixed protein and DNA were analysed by analytical gel filtration (a-SEC) using a Superdex 200 Increase 10/300 GL column (GE Healthcare) running in DNA binding buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... to get rid of the protease and further purified by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare). The purity of the recombinant protein was verified by SDS-PAGE and fractions with 90–95% purity were pooled and stored at –80°C.
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bioRxiv - Molecular Biology 2024Quote: ... Solid-phase syntheses of short oligonucleotides were performed in a 10 mL syringe equipped with frit and loaded with polystyrene support [ribo A 300 PrimerSupport 5G (299 μmol/g, GE Healthcare)] ...
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bioRxiv - Molecular Biology 2024Quote: ... This lysate was applied onto a 1 mL HisCap Smart 6FF column (Smart-Life Sciences) using an Akta Purifier 10 chromatograph (GE Healthcare). Subsequent washing steps were carried out with mix of buffer A and buffer B (buffer A + 500 mM imidazole) ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was desalted by a Zeba spin desalting column (7 kDa MWCO) and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare). Fluorescein- labeling efficiency was analyzed by SDS-PAGE and fluorescence gel imaging (Amersham Typhoon FLA 9500 ...
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bioRxiv - Molecular Biology 2024Quote: ... to get rid of the protease and further purified by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare). The purity of the recombinant protein was verified by SDS-PAGE and fractions with 90–95% purity were pooled and stored at –80°C.
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bioRxiv - Microbiology 2024Quote: ... The fractions containing L–P protein were further purified using a size-exclusion column (Superose 6 Increase 10/300 GL, GE healthcare) equilibrated with size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2024Quote: ... The molecular weight of protein complexes was determined by size exclusion chromatography performed on Superdex 200 Increase 10/300 column (GE Healthcare) calibrated with High Molecular Weight calibration kit (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... The protein eluted from the HisTrap HP column was further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was then passed through an analytical gel filtration column called Superdex 200 Increase 10/300 GL (GE Healthcare, USA) with the running buffer (25 mM Tris-HCl ...
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bioRxiv - Genetics 2024Quote: ... The membranes were subsequently washed three times for 10 minutes each with TBST after which signal development was performed with ECL Select Western Blotting Detection Reagent (GE Healthcare). Images were acquired using the ChemiDocImager (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was desalted by a Zeba spin desalting column (7 kDa MWCO) and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare). Fluorescein-labeling efficiency was analyzed by SDS-PAGE and fluorescence gel imaging (Amersham Typhoon FLA 9500 ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using the First-Strand cDNA Synthesis Kit (GE Healthcare). Quantitative RT–PCR was performed using qPCR MasterMix Plus Low ROX (Eurogentec ...
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bioRxiv - Cell Biology 2020Quote: ... The bands were visualized after incubation with ECL chemiluminescence kit (Amersham, GE Healthcare). An anti-tubulin antibody was used as a loading control (monoclonal mouse anti-a-Tubuline ...
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bioRxiv - Cell Biology 2020Quote: ... Revelation was performed using an enhanced chemiluminescence detection system (ECL kit, GE Healthcare). Primary antibodies used were ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were detected using enhanced chemiluminescence (ECL) detection kit (GE HealthCare, RPN2235).
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bioRxiv - Molecular Biology 2021Quote: ... purified with mini columns (GFX PCR DNA & gel band purification kit, GE Healthcare) is described in this protocol (dx.doi.org/10.17504/protocols.io.brzsm76e ...
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bioRxiv - Microbiology 2019Quote: Protein concentration was measured with the “2D Quant kit” (GE Healthcare Life Sciences), according to the supplier’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Revelation was carried out using the ECL Plus western blotting kit (GE Healthcare). Primary antibody used for western blotting (WB ...
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bioRxiv - Neuroscience 2021Quote: ... HRP-conjugated secondary antibodies signal was revealed using ECL Prime kit (GE Healthcare) and captured using the ChemiDoc imager (BioRad ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen revelation was performed using the ECL kit (GE Healthcare, Chicago, IL, USA) and images were acquired on a MyECL machine (Thermo Fisher Scientific ...
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The anti-lipidemic drug simvastatin modulates Epigenetic Biomarkers in the Amphipod Gammarus locustabioRxiv - Pharmacology and Toxicology 2020Quote: ... together with the illustraTM RNAspin Mini RNA Isolation Kit (GE Healthcare, United Kingdom), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The Amersham ECL Western Blotting Detection Reagent Kit (GE Healthcare Life Sciences, UK) was used for final detection of proteins on the membrane.
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bioRxiv - Immunology 2022Quote: ... A low molecular weight gel filtration calibration kit (product code-28403841) (GE Healthcare) was used for calibrating the column ...
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bioRxiv - Pathology 2021Quote: ... and then visualized using a Western Blotting Detection Kit (GE Healthcare, cat#: RPN2106). Antibodies used in this study are listed in Supplementary Table 2.
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bioRxiv - Molecular Biology 2020Quote: ... Immunoblots were analysed using Amersham™ ECL™ Prime Kit from GE Healthcare Life Sciences (Waukesha ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using 2-D quant kit (GE healthcare, Cat# 80648356), by following manufacturer’ instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was extracted using the illustra RNAspin Mini Isolation kit (GE Healthcare). Reverse transcription was performed using oligo-dT and random hexamer primers with an in-house enzyme produced by the Protein Technologies Unit at CRG ...
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bioRxiv - Microbiology 2022Quote: ... and amplicons were purified using the Gel Band Purification kit (GE Life Sciences), re-surveyed on Nanodrop® and delivered to DNA Sanger sequencing at “laboratório de sequenciamento de ácidos nucleicos da FCFRP-USP” (Brazil) ...
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bioRxiv - Microbiology 2022Quote: ... pneumophila gDNA was isolated using the Illustra genomicPREP DNA spin kit (GE Healthcare) and used as a template for cloning sidI and mesI into the indicated plasmid vectors ...
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bioRxiv - Biochemistry 2022Quote: Samples were precipitated with the SDS-PAGE Clean-Up Kit (10074304 GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... signals were revealed using the ECL Prime western blotting Detection Kit (GE Healthcare), and visualized with a ChemiDoc XRS Touch Imaging System (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Horseradish peroxidase conjugated secondary antibodies and enhanced chemiluminescence reagents (ECL kit) (GE Healthcare) were used for detection ...