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Citations for GE Life Sciences :
3201 - 3250 of 4095 citations for Cow Ataxin 10 ATXN10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The supernatant (post-vesicle fraction, PVF) was then fractionated with Superose 12 10/300 GL column (GE Healthcare, Little Chalfont, UK) (flow rate 0.4 mL/min ...
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bioRxiv - Microbiology 2023Quote: ... the recombinant proteins were purified from the supernatant using nickel-affinity chromatography (Ni-NTA agarose, ABT) followed by size exclusion chromatography (Superose 12 10/300 GL, GE Healthcare), except in the cases of AIP56N1-E307 and AIP56N1-E307DTR in which only nickel-affinity chromatography was performed ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were filtered and then pelleted at 300 g for 10 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 900 g for 20 minutes with acceleration set to 4 and deceleration set to 1 in order to remove cellular debris ...
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bioRxiv - Biophysics 2023Quote: ... Fractions containing the GBP1F were pooled and further purified by size exclusion chromatography on a Superdex200 Increase 10/300 GL column (GE Healthcare) in running buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Microbiology 2023Quote: ... The radiochemical purity was estimated to be > 95% based on TLC autoradiography (TLC-ARG) and HPLC (LC-20; Shimadzu) using a Superdex 200 10/300 column (GE Healthcare). 89Zr-DFO-3N39v4-Fc was administered into the tail vein (400ug ...
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bioRxiv - Microbiology 2023Quote: Further protein purification was achieved by size exclusion chromatography (SEC) through a Superdex™ 200 10/300 GL column (GE Healthcare) using SEC buffer containing 0.5 M NaCl and 25 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... IgGs were expressed by transient transfection in Expi293 cells or HEK293-6E cells and purified from cell supernatants using MabSelect SURE (Cytiva) columns followed by SEC purification using a 10/300 or 16/600 Superdex 200 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: For determination of molecular masses purified LS variants were separated in analytical size exclusion chromatography (SEC) column Superdex 200 10/300 (GE Healthcare). Superose 6 increase 10/300 (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... To insure the monodispersity of samples all proteins were subjected to SEC directly before collecting of scattering data on a Superdex 200 10/300 GL (GE Healthcare) equilibrated with 20 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... equilibrated with buffer A (50 mM Tris-HCl at the appropriate pH, 300 mM NaCl) on an AKTÄ explorer 10S FPLC system (GE Healthcare). The column was washed with 10 column volumes of buffer A ...
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bioRxiv - Biophysics 2022Quote: ... All BtuF constructs were purified directly from whole cell lysate (obtained by sonication) via Ni-NTA chromatography followed by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) in final buffers of 20-50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... Size exclusion chromatography was performed on the concentrated protein using a Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) equilibrated in gel filtration buffer (10 mM Na2HPO4.7H2O ...
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bioRxiv - Biophysics 2023Quote: ... Samples were injected on the Äkta prime plus chromatography system (Cytivia) and separated on a Superose 6 10/300 column (GE Healthcare) in SEC buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: Proteins were separated using SDS-PAGE in 10 % Bis-Tris acrylamide/ bisacrylamide gels and transferred to a nitrocellulose membrane (GE Healthcare). The membranes were blocked for 30 minutes with PBS 5 % milk and incubated with the first antibody for 1 hour at room temperature or at 4°C over-night with gentle shaking ...
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bioRxiv - Biochemistry 2023Quote: ... of GcapOCP2 or CrinOCP2 holoform samples (1 mg/ml in 100 μl) was carried out on a Superdex 200 Increase 10/300 column (GE Healthcare) connected to a tandem of consecutive detectors Prostar 335 (Varian ...
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bioRxiv - Biochemistry 2023Quote: ... then ACKR3 was purified by size exclusion chromatography (SEC) using a Superdex 200 Increase (10/300 GL column) connected to an ÄKTA purifier system (GE Healthcare) and eluted in buffer elution buffer without Flag peptide or VUF11207 ...
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bioRxiv - Biochemistry 2023Quote: ... The eluate was then further purified by size exclusion chromatography using a Superdex 200 Increase (10/300 GL column) connected to an ÄKTA purifier system (GE Healthcare) in a buffer containing 20mM Hepes (pH 7.5) ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were further purified by anion exchange (HiTrap Q, Cytiva) or SEC (Superdex 200 Increase 10/300 GL, Cytiva) chromatography at ÄKTA FPLC (system ÄKTA pure; GE Healthcare). The purity of the protein was checked by the SDS PAGE.
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bioRxiv - Neuroscience 2023Quote: ... and pellets were resuspended in 6 mL 25 % Standard Isotonic Percoll (Percoll: GE Healthcare, 17-5445-02 with 10% 10x PBS) in Medium A ...
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bioRxiv - Cell Biology 2023Quote: ... The GST-tagged Bridge domain was purified by affinity chromatography with glutathione-SepharoseTM 4B resins (Cytiva) followed by size exclusion chromatography with Superose 6 10/300 (GE Healthcare) in the running buffer containing 150 mM NaCl and 20 mM HEPES (pH 7.5).
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bioRxiv - Immunology 2023Quote: ... We separated pFab and Fc from undigested IgG using size exclusion chromatography with a Superdex 200 Increase 10/300 column (GE Healthcare) and concentrated the pFab/Fc.
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bioRxiv - Neuroscience 2023Quote: ... The total volumes of soluble brain extracts were separated by SEC as previously described [17] on a single Superdex200 10/300GL column (no. 17-5175-01, GE Healthcare) in PBS (no ...
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bioRxiv - Biochemistry 2023Quote: ... the protein solution was concentrated to ∼6 mL using a centricon (MW cutoff = 10 kDa) and was purified via size exclusion chromatography with a HiLoad 26/600 Superdex 75 pg column (GE Healthcare). The purified protein solution was diluted to 135 μM (∼2.0 mg/mL ...
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bioRxiv - Biophysics 2023Quote: ... The successfully cleaved samples were subsequently buffer exchanged using a Superdex S200 5/150 GL or 10/300 increase column (GE Healthcare) connected to an Agilent HPLC 1260 Affinity equipped with a fraction collector ...
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bioRxiv - Biophysics 2023Quote: ... 3,000 g × 15 min at 4 °C) and further purified by size-exclusion chromatography (ÄKTA pure system, Superdex-75 Increase 10/300 GL, GE Healthcare). The unfolding and refolding process for GlnBP WT was conducted under the same conditions as described for the GlnBP variants.
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bioRxiv - Biophysics 2023Quote: ... pH 7.4 400 mM Imidazole) The labeled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex-75 Increase 10/300 GL, GE Healthcare) to eliminate remaining fluorophores and remove other contaminants and soluble aggregates ...
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bioRxiv - Biophysics 2023Quote: ... the protein was diluted 10 times with PBS buffer and immobilized on a Nickel Sepharose 6 Fast Flow resin (GE Healthcare). The resin was washed extensively with milliQ water followed by PBS buffer pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... The flow through was then concentrated in an Amicon Ultra-15 50,000 MWCO spin concentrator to ∼10-20 mg/mL and loaded onto a HiPrep 26/60 S200 Size Exclusion Column (GE Healthcare), equilibrated in Buffer D ...
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bioRxiv - Molecular Biology 2023Quote: ... T7 RNAP-based in vitro transcription was performed as described previously.10 The produced λtR1 rut site RNA was treated with DNase I and purified by strong anion-exchange (MonoQ, GE Healthcare) and size-exclusion chromatography (Superdex 75 ...
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bioRxiv - Molecular Biology 2023Quote: ... The following morning unbound G protein and Nb35 were separated from the complex by SEC on a Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with the buffer containing 20 mM HEPES/KOH pH7.5 ...
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bioRxiv - Biochemistry 2023Quote: PrgB188-1233 dimer fractions were thawed on ice and loaded on a Superdex 200 10/300 GL gel filtration column (GE Healthcare) equilibrated in 20 mM HEPES/NaOH pH 7.0 and 150 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer with 1 mM MgCl2 and 0.03% digitonin ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer with 0.03% digitonin ...
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bioRxiv - Biochemistry 2023Quote: ... M was concentrated to 2 ml using Vivaspin20 columns (SartoriusStedimBiotec) and purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50 mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Biochemistry 2023Quote: SUV420H1-H2A.Z nucleosome complex was assembled in a ratio of 3:1 and was purified on HiLoad Superdex 200 10/300 (GE Healthcare) size-exclusion liquid chromatography column (Buffer ...
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bioRxiv - Biochemistry 2022Quote: ... NSs::6xHIS was concentrated using a 10 kDa MWCO spin concentrator (Amicon) and further purified by size exclusion chromatography over a Superdex 200 Increase 10/300 GL column (GE Healthcare) in Elution Buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated with a 100 kDa cutoff concentrator to 500 μL and loaded on a Superose 6 Increase 10/300 column (GE Healthcare) equilibrated with gel filtration buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... The protein eluate was concentrated and further purified by size-exclusion chromatography on a Superose6 10/300 GL column (GE Healthcare). Peak fractions were collected for functional assay.
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bioRxiv - Biochemistry 2022Quote: ... NSs::6xHIS was concentrated using a 10 kDa MWCO spin concentrator (Amicon) and further purified by size exclusion chromatography over a Superdex 200 Increase 10/300 GL column (GE Healthcare) in Elution Buffer (20 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: Cellular lysates were separated on a Superose 6 HR 10/30 size-exclusion column and an AKTA Purifier protein purification system (GE Healthcare), essentially as described previously(61) ...
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bioRxiv - Biochemistry 2022Quote: ... The ACE2-induced postfusion S-nanodisc sample was purified by gel filtration chromatography on Superose 6 Increase 10/300 column (GE Healthcare) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... the protein was loaded through a Ni-NTA column before a size-exclusion chromatography using Superdex 200 increase 10/300 GL column (GE Healthcare) in G-buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were resolved by electrophoresis on 10 or 12% SDS-PAGE gels and transferred onto polyvinylidene fluoride (PVDF) membranes (GE Healthcare). Total protein labeling was performed using No Stain Labeling Reagent (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... Eluted protein was concentrated and subjected to size-exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare, 289909944) that was pre-equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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bioRxiv - Biophysics 2023Quote: ... The concentrated protein sample was further purified by size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 GL (GE Healthcare) column pre-equilibrated with the buffer E (20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... concentrated using a 30 kDa spin concentrator (Amicon) and subjected to size-exclusion chromatography using a Superdex 200 increase 10/300 GL column (GE Healthcare) in a buffer 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... All BtuF constructs were purified directly from whole cell lysate (obtained by sonication) via Ni-NTA chromatography followed by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) in final buffers of 20-50 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... The reaction was mixed for 2 hours at room temperature (RT) and then 4M NaCl was added to the derivatized fVi for purification by PD-10 column (GE Healthcare) against water ...