Labshake search
Citations for GE Life Sciences :
2951 - 3000 of 4765 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... The overexpressed proteins were bound to the washed Ni2+-chelating Sepharose (GE Healthcare, Pittsburgh, PA, USA). The suspension was mixed on a three-dimensional rotating mixer for 40 min at 4 °C ...
-
Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... proteins were electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 90 V for 90 min on ice in transfer buffer (48 mM Tris and 39 mM glycine supplemented with 20% (v/v ...
-
bioRxiv - Genetics 2024Quote: ... The proteins were electrophoresed on 10% SDS□PAGE gels and transferred to nitrocellulose membranes (GE Healthcare). The blots were blocked in 5% milk and incubated with primary antibodies overnight at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare) were performed according to our published procedure (Ninagawa et al. ...
-
bioRxiv - Genomics 2023Quote: ... 167 mM NaCl) and pre-cleared with protein-A-Sepharose beads (GE Healthcare 17-5280-02) for 30 min at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lysate containing the protein of interest was loaded onto a 5 mL HisTrap column (GE Healthcare) equilibrated in 200 mM potassium phosphate ...
-
bioRxiv - Immunology 2023Quote: ... Proteins were transferred onto an Amersham™ Protran™ 0.45 μm nitrocellulose membrane (GE Healthcare, UK). Membranes were blocked with BlueBlock PF (Serva Electrophoresis GmbH ...
-
bioRxiv - Microbiology 2023Quote: ... The protein was further purified on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) equilibrated in 10 mM HEPES/NaOH pH=8.0 ...
-
bioRxiv - Immunology 2022Quote: ... Th gB-1 mRNA was purified by fast protein liquid chromatography (FPLC; Akta purifier; GE Healthcare)79 ...
-
bioRxiv - Microbiology 2023Quote: ... The proteins separated by the SDS-PAGE were transferred to a nitrocellulose membrane (GE life Sciences). The membrane was then incubated for 1 h at room temperature or overnight at 4 °C in blocking buffer (5% non-fat milk powder diluted in TBST (20 mM Tris ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated using 12 %SDS-PAGE and blotted onto a polyvinylidene difluoride membrane (GE Healthcare). The membrane was blocked in 5% milk/PBS for 1 h followed by overnight incubation with mouse monoclonal anti-TFAM antibody (Abcam ...
-
bioRxiv - Neuroscience 2023Quote: ... The resolubilized protein was bound to QFF anion exchange resin (GE Healthcare Life Sciences, Marlborough, MA) and eluted using a linear gradient of 0.2–0.6 M NaCl ...
-
bioRxiv - Microbiology 2023Quote: ... protein was concentrated and further purified by size-exclusion chromatography in a Superdex-S200 (GE Healthcare) column equilibrated with 50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were concentrated and further purified by size-exclusion chromatography in a Superdex-S200 (GE Healthcare) column using 50 mM Tris-HCl pH 8.0 ...
-
Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulationbioRxiv - Biochemistry 2023Quote: ... Eluted protein was applied to a HiLoad 26/600 Superdex 200 pg column (GE life sciences) equilibrated with 150mM NaCl ...
-
bioRxiv - Neuroscience 2023Quote: ... The monomeric proteins were then purified by using the HisTrap nickel column (GE Life Sciences 17524701) for FPLC (ÄKTA start protein purification system ...
-
bioRxiv - Cell Biology 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... 25µL of Protein L beads (Cat. no. Capto™ L resin, GE Healthcare Cat. no. 17547802), pre-equilibrated with binding buffer (20mM HEPES ...
-
bioRxiv - Biophysics 2023Quote: ... Soluble protein was maintained at 4°C and purified using Ni-NTA affinity resin (GE Healthcare). A column wash was performed using pH 7.0 HisA containing 300 mM NaCl ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Genomics 2023Quote: ... Lysates were pre-cleared with 30µl of either protein-A-sepharose (GE Healthcare, 17-5280-01) or protein-G-sepharose beads (GE Healthcare ...
-
bioRxiv - Biophysics 2023Quote: ... The protein solution was passed over two coupled HiTrap Desalting Columns (GE Life Sciences, Pittsburgh, PA) to remove DTT and buffer exchanged into 20 mM Tris (pH 7.4) ...
-
bioRxiv - Biophysics 2023Quote: ... The protein was purified by affinity chromatography using the Ni2+-Sepharose fast flow resin (GE Healthcare), pre-equilibrated with 10 column volumes of buffer A containing 1 mM DTT and gravity loaded with the supernatant from the preceding ultra-centrifugation step ...
-
bioRxiv - Biochemistry 2023Quote: ... the concentrated protein was applied onto a Superose 6 Increase 10/300 GL column (GE healthcare) equilibrated with SEC buffer (20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Neuroscience 2023Quote: ... The eluted proteins were further purified via gel filtration (Superdex 200, GE Healthcare Chicago, IL, USA). In all cases ...
-
bioRxiv - Neuroscience 2023Quote: ... The eluted proteins were further purified via gel filtration (Superdex 200, GE Healthcare Chicago, IL, USA). In all cases ...
-
bioRxiv - Neuroscience 2023Quote: ... and Synaptotagmin-1 protein was subjected to the anion exchange column (MonoS, GE Healthcare, Chicago, IL) to remove nucleotide contaminants ...
-
bioRxiv - Molecular Biology 2023Quote: ... The dialyzed protein was concentrated and injected into a HiLoad S75 16/600 column (GE Healthcare) pre-equilibrated with GST SEC buffer ...
-
bioRxiv - Systems Biology 2023Quote: ... proteins in 2% SDS were bound to paramagnetic beads (SeraMag Speed beads, GE Healthcare,#45152105050250,#651521050502) by addition of ethanol to a final concentration of 50% ...
-
bioRxiv - Microbiology 2023Quote: ... proteins were loaded into a size exclusion chromatography column Superdex 200 increase 10/300 (GE Healthcare). The protein concentration was determined by the Bradford method.
-
bioRxiv - Biophysics 2023Quote: ... Protein was further purified via size exclusion chromatography using a Superose6 Increase column (GE Healthcare/Cytiva) preequilibrated with aforementioned buffer without imidazole addition ...
-
bioRxiv - Biophysics 2023Quote: ... and the protein purified by Ni2+-affinity chromatography using an Äkta FPLC Chromatography System (GE Healthcare). The fusion proteins were eluted with a gradient of 1 M imidazole and dialyzed into 20 mM Tris (pH 7.4 ...
-
bioRxiv - Biochemistry 2023Quote: ... Protein was released from the GST beads by thrombin cleavage and purified by MonoQ (GE Healthcare) ion-exchange chromatography ...
-
bioRxiv - Biophysics 2023Quote: ... Proteins were then subjected to size-exclusion chromatography using a HiLoad Superdex 200 column (GE Healthcare) in a 20 mM Tris ...
-
bioRxiv - Cell Biology 2022Quote: ... the protein-DNA complex was further purified using Hiload 16/60 Superdex 75 column (GE Healthcare). Then ...
-
bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred to a nitrocellulose membrane (Amersham Hybond ECL; GE Healthcare, RPN303D, Merck, Darmstadt, Germany). After blocking in 5% (w/v ...
-
bioRxiv - Immunology 2023Quote: ... Protein purification was performed using glutathione sepharose 4B resin according to the manufacturer’s instructions (GE Healthcare). Eluted proteins were then dialyzed and concentrated using an Amicon Ultra-15 10 KDa cutoff (Millipore).
-
bioRxiv - Immunology 2023Quote: ... Eluted protein was concentrated with 3 kDa MWCO filters (Amicon) before size-exclusion chromatography (GE Healthcare). Proteins were stable at 4ºC.
-
bioRxiv - Immunology 2023Quote: ... The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe, GE Healthcare), followed by a preparative size exclusion chromatography (SEC) ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein-containing fractions were pooled and further purified on a Superdex 200 column (GE Healthcare), equilibrated ...
-
bioRxiv - Developmental Biology 2023Quote: ... The proteins were then transferred from the gel to the PVDF membrane (GE Healthcare Life-Science) in Bio-Rad vertical gel Transfer Apparatus using Transfer buffer (2.5mM Tris base ...
-
bioRxiv - Biochemistry 2023Quote: ... The proteins were further purified by gel filtration on Superdex 75 pg 16/600 (GE Healthcare) in 25 mM Tris-HCl ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein detection was finally performed using Amersham ECL Western Blotting detection reagent (GE Healthcare #CA95038-564L).
-
bioRxiv - Immunology 2023Quote: ... The proteins were visualized by ECL-Plus (GEHealthcare) using Image Quant LAS500 (GE Healthcare Life Sciences). The following antibodies were used ...
-
bioRxiv - Plant Biology 2023Quote: Protein samples were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). After being blocked in 1 × PBST buffer containing 5% skimmed milk ...
-
bioRxiv - Plant Biology 2023Quote: ... The protein concentration was determined with 2-D Quant Kit (GE Healthcare Life Sciences, Westborough, USA) using Bovine Serum Albumin (BSA ...
-
bioRxiv - Cell Biology 2024Quote: ... the proteins of interest were revealed by chemiluminescence using the ECL WesternBlot detector kit (GE Healthcare) and the results were digitised in a UVITEC11Photo Imager (Ubitec Photoimager ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and the supernatant was collected for protein purification with Ni-NTA columns (GE Healthcare, Uppsala, Sweden). The proteins were dialyzed with PBS at 4 °C and concentrated.
-
bioRxiv - Molecular Biology 2023Quote: ... A small portion of the final proteins were run through the Superdex 200 columns (GE Healthcare) again for quality check.
-
bioRxiv - Immunology 2023Quote: ... Samples were pre-cleared with 40 µl of protein G Sepharose beads (50% slurry, GE Healthcare) plus 5 μg Goat IgG (Invitrogen) ...