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Citations for GE Life Sciences :
2901 - 2950 of 4765 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the protein was cleaned up using a Superdex 75 Increase 10/300 GL column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 8-15% SDS gels and transferred to a nitrocellulose membrane (GE Healthcare) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were electrophoretically transferred onto the nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 100 V for 90 min on ice in the transfer buffer (48 mM Tris–HCl and 39 mM glycine) ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT) and bound protein was eluted directly onto a HiTrap Heparin column (GE Healthcare) using buffer Hep A supplemented with 2.5 mM Desthiobiotin ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were detected using Amersham enhanced chemical luminescence reagent as per the manufacturer’s instructions (GE Healthcare) and visualized by exposure to x-ray film.
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bioRxiv - Cell Biology 2021Quote: ... Full-length IgG was obtained by affinity purification utilizing a Protein A chromatography column (GE Healthcare) in AKTA avant (Cytiva) ...
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bioRxiv - Synthetic Biology 2021Quote: ... proteins were further purified by gel-filtration on a 10/300 Superdex S75 column (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... Protein purification was performed via affinity chromatography using Ni2+ charged Fast Flow Chelating Sepharose (GE Healthcare) as stationary phase ...
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Structure of mycobacterial cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidatesbioRxiv - Microbiology 2021Quote: ... Protein was then concentrated and loaded onto a Superdex 6 increase (10/300 GL, GE Healthcare) column equilibrated in buffer B ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was concentrated and purified over HiTrap Protein G HP antibody purification column (GE Healthcare). To visualize 410C9 in vivo ...
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bioRxiv - Neuroscience 2020Quote: ... The eluted protein was subjected to MonoQ 10/100 GL (GE Healthcare Lifesciences, Marlborough, MA, USA) chromatography using a 0-600 mM NaCl gradient elution ...
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bioRxiv - Biochemistry 2021Quote: ... The protein mixture was then purified through two tandem 5-ml MBPTrap HP columns (GE Healthcare). After loading ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins in the gel were transferred to polyvinylidene fluoride (PVDF) membrane (GE Healthcare Life Sciences). The membranes were blocked with 2% milk (Marvel ...
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bioRxiv - Biochemistry 2021Quote: ... The size exclusion chromatography was run with the ÄKTA protein purification system (GE Healthcare Life Sciences), equipped with Superdex 75 10/300 GL and run with buffer solution (150 mM sodium chloride ...
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bioRxiv - Immunology 2020Quote: ... The concentrated protein elutes were run on a Superdex 200 Increase 10/300 GL (GE Healthcare) column on an Akta™Pure (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... dialyzed protein was transferred into Lysis Buffer with a HiPrep 26/10 desalting column (GE Healthcare) and passed over a HisTrap FF column ...
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bioRxiv - Immunology 2021Quote: ... mAbs were purified directly from culture supernatants using HiTrap protein G columns (GE Healthcare Life Sciences) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... The proteins were purified from the cell lysate using 300 μl GST resin (GS4B, GE Healthcare), and incubated with excess purified wild-type or mutant rDPP9 proteins on ice for 60 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μl of bait protein was mixed with 40 μl bed volume StrepTactin resin (GE Healthcare) and incubated for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The untagged protein was applied to the Superdex 200 increase 10/300 GL column (GE Healthcare) in SEC-buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted protein was concentrated and subjected to a HiLoad 16/600 Superdex 75 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was purified using Protein G Sepharose 4 Fast flow (GE Healthcare Life Sciences, Pittsburgh, PA) as per manufacturer protocol.
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was concentrated and subjected to a HiLoad 16/600 Superdex 75 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... the protein without His6-tag was injected into a Superdex 75 10/300 GL (GE Healthcare) size exclusion column with the buffer B (50 mM NaH2PO4/Na2HPO4 pH 6.5 ...
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bioRxiv - Neuroscience 2022Quote: ... The eluted protein was concentrated and further injected into a Superose 6 Increase column (GE Healthcare) for size-exclusion chromatography (SEC ...
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bioRxiv - Developmental Biology 2022Quote: ... The proteins were then transferred onto a Protran Nitrocellulose Membranes (0.45 µm pore size, GE Healthcare) via Power Blotter-Semi-dry Transfer System (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Abcam prestained mid-range protein ladder (Ab116027) and 0.45 µM Amersham Hybond PVDF membrane (GE Healthcare) were used ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein-containing fractions were pooled and further purified on a Superdex 200 column (GE Healthcare), equilibrated and eluted in tubulin buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein purification was performed on HisTrap HP 5 ml Ni-based column (Cytiva, former GE Healthcare) using ÄKTA Explorer System (Cytiva ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred (100 V, 1 hr) onto PVDF (polyvinylidene difluoride) membranes (GE Healthcare Life Sciences) using 1X Tris/Glycine buffer (in-house 50X solution containing 250 mM Tris and 1920 mM Glycine) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was resuspended in lyses buffer and inject into a Superdex 200 26/100 (GE Healthcare) pre-equilibrated with gel filtration buffer (20 mM Tris pH 7.8 ...
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bioRxiv - Biochemistry 2022Quote: ... cleaved protein was further purified by gel filtration using a Sephacryl S-400 column (GE Healthcare) with one of two buffers ...
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bioRxiv - Cell Biology 2022Quote: ... and protein expression was visualized using an enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK) as described before88 ...
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bioRxiv - Physiology 2022Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred onto nitrocellulose membranes (GE Healthcare). The nitrocellulose membranes were blocked with 5% BSA in TBST (TBS containing 0.1% Tween20 ...
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bioRxiv - Microbiology 2022Quote: ... M28 IgG was then purified from the supernatant using Protein A affinity chromatography (GE Healthcare #17549851). IgG was then dialyzed overnight in TBS (150mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... Quantifications of protein levels were performed with an ImageQuant™ LAS 4000mini Biomolecular Imager (GE Healthcare), and images were analyzed with ImageJ software (NIH) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein constructs were concentrated with Vivaspin 20 5000molecular weight cut-off centrifuge tubes (GE Healthcare) and stored at -80 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were purified from cell extracts using MBPTrap™ HP column (GE Healthcare, Chicago, IL, USA) with ÄKTA FPLC system (Amersham Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... proteins were electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 90 V for 90 minutes on ice in transfer buffer (48 mM Tris base and 39 mM glycine supplemented with 20% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The protein was further purified using a Superdex 75 26/60 gel filtration column (GE healthcare) preequilibrated with buffer C (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Physiology 2022Quote: ... All proteins were further polished with Superdex 200 Increase 10/300 GL (GE Healthcare Life Sciences) size-exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were loaded on a 5 ml HisTrap™HP column (1 mL/min; GE Healthcare) in a binding buffer containing 100 mM tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was fitered and protein purification was conducted using a Resource Q column (GE Healthcare) equilibrated in Buffer A ...
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bioRxiv - Cell Biology 2024Quote: ... Labelled protein bands were detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, USA) and ChemiDoc MP Imaging System (Bio-Rad ...
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bioRxiv - Synthetic Biology 2023Quote: ... All proteins were further purified with Superdex 200 Increase 10/300 GL (GE Healthcare Life Sciences) size-exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2024Quote: ... the protein was purified by size exclusion chromatography using a Sephadex 200 16/60 (GE Healthcare) column with the lysis buffer.
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bioRxiv - Biophysics 2024Quote: ... A calibration curve was produced for eluting species using known protein standards (GE Healthcare Life Sciences). The dimerisation-impaired D123P construct of Ire1-LD was used to assign the apparent molecular weight of eluted monomeric protein ...
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bioRxiv - Microbiology 2024Quote: ... and the surface proteins were bound to streptavidin Sepharose beads overnight at 4°C (GE Healthcare). Beads were then washed with buffer containing 100 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... and the proteins were then transferred onto nitrocellulose blotting membranes (NC) (10600001; GE Healthcare Life Science). The membranes were blocked with 5% non-fat dry milk and 0.1% Tween 20 for 30 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... GST or the GST fusion protein was purified using a glutathione Sepharose 4B column (GE Healthcare). GFP::RAB-5 protein from wild-type or usp-50(xd413 ...