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Citations for GE Life Sciences :
2801 - 2850 of 4765 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... overnight and protein was further purified over a Superdex 75 16/600 pd column (GE Healthcare) equilibrated in 50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare 10600019 or 10600001). Appropriate transfer of proteins was verified by staining of membranes with the Ponceau S solution (Roth 5938.1) ...
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bioRxiv - Bioengineering 2020Quote: ... The supernatants were purified using a peristaltic pump and a HiTrap Protein A column (GE Healthcare). Fc-fused proteins were acid-eluted into 1X PBS ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified from the cell-free extract using an HisTrap HP column (GE Healthcare) and a linear imidazole gradient (30 to 500 mM ...
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bioRxiv - Biochemistry 2021Quote: ... and protein loaded onto a GSTrap FF 5 mL column (GE Life Sciences, Marlborough, MA, USA) using the NGC Chromatography system (Bio-RAD) ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was fractionated on a HiLoad 16/600 Superdex 200 gel filtration column (GE Healthcare), eluting with SEC buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was loaded onto a Superdex 75 10/300 gel filtration column (GE Healthcare, USA), which had been equilibrated in buffer containing 20 mM Tris pH 8.0 and 100mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare). The protein was dialyzed against 1X PBS overnight in the presence of 1:1000 (w/w ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare) in 1XPBS pH 7.5 and eluted with 300 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified using anion exchange chromatography (Mono Q 5/50 GL, GE healthcare) pre-equilibrated with ion exchange buffer A (20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The flow-through fraction containing target proteins were collected and purified with Superdex 75 (GE Healthcare) chromatography ...
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bioRxiv - Biochemistry 2020Quote: ... After electrophoresis the proteins were transferred to Nitrocellulose membranes (Amersham Protran Premium Nitrocellulose, GE Healthcare, #10600048) via wet transfer at a current of 45V for approximately 20 hours at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Eluted protein was further purified by size exclusion chromatography using a Superdex200 column (GE Life Sciences) in 25 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... Diluted protein was subjected to anion exchange chromatography using a HiTrapQ HP column (GE Life Sciences) and eluted in 20 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were electrophoretically transferred onto the nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 μm NC) at 100 V for 90 min on ice in the transfer buffer (48 mM Tris–HCl and 39 mM glycine) ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 μm NC) at 90 V for 90 min on ice in transfer buffer (48 mM Tris-HCl and 39 mM glycine supplemented with 20% methanol) ...
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bioRxiv - Biophysics 2020Quote: ... cells were pelleted and the supernatant was loaded onto a HiTrap Protein A column (GE Healthcare) following the manufacturers protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... 35S-labelled proteins were monitored using autoradiography and visualised using a Typhoon 9400 scanner (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... The separated proteins were transferred to a 0.2 µm nitrocellulose membrane (Amersham Protran, GE Life Sciences) using wet transfer method for 2 h at 200 mA ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was visualized by Amersham enhanced chemiluminescence (ECL) Western Blotting Detection Reagent (GE Healthcare Life Sciences) and X-ray film (Fujifilm SuperRX) ...
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bioRxiv - Cancer Biology 2020Quote: ... Membrane-immobilized protein detection used ECL Western Blotting Detection Reagents (GE Healthcare RPN2106; Millipore Sigma GERPN2106).
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were separated by SDS-PAGE and were transferred onto PVDF membranes (GE Healthcare Life sciences) for immunoblot analysis ...
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bioRxiv - Biochemistry 2021Quote: ... and protein fractions were separated using a Superdex 200 10/300 column (GE Healthcare Life Sciences) with 50mM Tris (pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1mg of purified antibody was injected into the AKTA protein purification system (GE Healthcare Life Sciences) and protein fractions were separated using a Superdex 200 10/300 column (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μg of each protein extract were concentrated using the 2D Clean-up kit (GE Healthcare) and then resuspended in 30 mM Tris pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins (20-50 μM) were injected onto Superdex-200 Increase 10/300 column (GE Healthcare) for analysis at a flow rate of 0.5 mL min−1 ...
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bioRxiv - Cell Biology 2021Quote: ... The eluted proteins were then separated by Superdex 200 Increase 10/300 GL column (GE Healthcare) that was equilibrated in size exclusive buffer (SEC ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were run on a 10 % SDS-PAGE and immobilized on a nitrocellulose membrane (GE Healthcare) using the Trans-Blot Turbo system (BioRad) ...
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bioRxiv - Biophysics 2021Quote: ... The protein was purified by IEX on a HiPrep Q FF anion exchange column (GE Healthcare) and then further purified by SEC on a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare) ...
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bioRxiv - Systems Biology 2020Quote: ... Tag-free NCK2 proteins were further purified using a 1 ml HisTrap FF column (GE Healthcare) to remove free His-tagged GST and TEV as well as remaining impurities ...
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bioRxiv - Molecular Biology 2020Quote: ... and the eluted protein was further purified through 5 mL HiTrap Q HP column (GE Healthcare). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... The solubilized protein was then incubated with Sepharose-4B resin (GE Healthcare Life Sciences, Pittsburgh, PA) conjugated with monoclonal α-1D4 antibody (provided generously by Prof ...
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bioRxiv - Microbiology 2021Quote: ... and 10 µL of packed protein A sepharose beads (GE Healthcare cat. no. 17-0618-01) were then added ...
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bioRxiv - Microbiology 2021Quote: ... The target protein was further purified by size exclusion chromatography (Superdex 200 [16/60], GE Healthcare) in the buffer (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, United Kingdom) in a wet tank unit (Bio-Rad ...
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Cytokinin-promoted secondary growth and nutrient storage in the perennial stem zone of Arabis alpinabioRxiv - Plant Biology 2020Quote: ... Total protein contents were assayed using the 2-D Quant Kit (GE Healthcare, Little Chalfont, UK), measuring OD480 in a microplate reader (Tecan Group ...
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bioRxiv - Systems Biology 2021Quote: ... The proteins were detected by Enhanced Chemiluminescence (ECL) using ECL Western Blotting Reagents (Amersham, GE Healthcare) or SuperSignal West Dura Extended Duration Substrate (Pierce).
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bioRxiv - Biochemistry 2021Quote: ... All eluted proteins were further purified on a SuperdexTM 75 10/300 GL column (GE Healthcare). The purified proteins were analyzed by SDS-PAGE and MALDI-TOF and concentrated using ultra-filtration through a 3 kDa cut-off Amicon (Millipore ...
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bioRxiv - Cancer Biology 2020Quote: ... The remaining portion was precleared using protein A Sepharose GL-4B beads (GE Healthcare, Piscataway, NJ) and incubated overnight with an antibody directed toward a specific histone modification ...
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bioRxiv - Biophysics 2020Quote: ... the reducing agents in the protein solution were removed with a PD10 desalting column (GE Healthcare), using a buffer based on 50 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... the protein was further purified using a size-exclusion column (Superdex, GE Healthcare, Chicago, Illinois, USA).
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bioRxiv - Biophysics 2020Quote: ... The proteins were purified by successive passage through HiTrap heparin and Superdex 75 columns (GE Healthcare). The nsp16/nsp10 complex was eluted as a single homogenous species in a final buffer containing 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... The protein was isolated by affinity chromatography (5 ml HisTrap, GE Healthcare Life Science, Chicago, USA) and desalted (5 ml HiTrap ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was loaded into a 5 mL HisTrap FF column charged with Ni2+ (GE Healthcare). The protein was eluted using a buffer containing 10 mM tris (pH 8.0) ...
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bioRxiv - Immunology 2022Quote: ... mAbs were harvested and purified 7 days after transfection using Protein G Sepharose columns (GE Healthcare). All purified recombinant mAbs were analyzed by SDS-PAGE under reducing conditions using NuPAGE Novex 4-12% Bis-Tris polyacrylamide gels and NuPAGE reducing agent (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... the protein samples were concentrated and injected into a Superdex 75 Prep Grade column (GE Healthcare) in a buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Genomics 2022Quote: ... All BPTF proteins were then further purified using anion exchange (Source 15Q, GE Healthcare Life Sciences) and size exchange chromatography (Superdex 75 ...
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bioRxiv - Physiology 2022Quote: ... The interactions between RS and proteins were measured using the BIAcore 3000 SPR system (GE Healthcare). The protein samples were diluted in HBS-EP buffer (0.01 M HEPES ...
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bioRxiv - Microbiology 2022Quote: ... The protein was affinity purified on a Ni2+-column (HiTrap IMAC HP, GE Healthcare Life Sciences). The eluted protein was cleaved with Rhinovirus 3C protease overnight and the cleaved complex was loaded onto a second Ni2+-column and the flow-through collected and further purified by size exclusion chromatography (Superdex 200 ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were mixed 2 h at 4°C with Protein-A-conjugated sepharose beads (GE Healthcare) and anti-Myc antibody (9B11 ...