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Citations for GE Life Sciences :
2851 - 2900 of 4765 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the protein was loaded into a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: ... We further purified the proteins through a 25 mL Superdex 200 preparatory-grade column (GE Healthcare) with 20 mM Na2HPO4 and 100 mM NaCl (pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for separation of proteins which were transferred to nitrocellulose blotting membrane (GE Healthcare, #10600002). Following washing with 0.05% Tris buffered saline-Tween (TBST) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified via loading onto a 5 mL HisTrap HP column (GE Healthcare) and pooling the untagged elution ...
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bioRxiv - Biochemistry 2022Quote: ... all MIT proteins were purified by Superdex 75 gel filtration chromatography (GE Healthcare Life Sciences, USA) in 25 mM Tris (pH 7.2 at 25 °C) ...
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bioRxiv - Plant Biology 2021Quote: ... Then the protein complexes were loaded onto a Superdex 200 PC 3.2/10 column (GE Healthcare) at a flow rate of 0.05 ml/min ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred onto Amersham Hybond 0.2 PVDF blotting membranes (Cat# 10600021, GE Healthcare Life Sciences) in Tris glycine buffer (25 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... Total protein was quantified using the PlusOne 2D Quant Kit (GE Healthcare Europe GmbH, Freiburg, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag fused proteins were purified by affinity chromatography column with GSTrap HP (GE Healthcare) and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated on a 10% SDS-PAGE and immobilized on a nitrocellulose membrane (GE Healthcare) using the Trans-Blot Turbo system (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... and POLGARF bound proteins were eluted by incubation with 1 μl of Prescission Protease (GE Healthcare) in PBS at 4°C overnight ...
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bioRxiv - Biophysics 2020Quote: ... the proteins were further purified by size exclusion chromatography (Superdex 200 16/60 HL, GE Healthcare) and eluted in 20 mM HEPES pH 7.6 at 8 °C ...
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bioRxiv - Immunology 2020Quote: ... The supernatant from pDNA transfected cells was purified using Protein G GraviTrap (GE Healthcare, Chicago, IL) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... the antibodies secreted into the supernatant were collected and captured by protein A Sepharose (GE Healthcare). The bound antibodies were eluted and further purified by gel-filtration chromatography using a Superdex 200 High Performance column (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The target proteins were further purified through a Superdex200 10/300 gel filtration column (GE Healthcare) in a buffer containing 20 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... and the protein concentrations were determined using a 2-D Quant Kit (GE Healthcare; Piscataway, USA).
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bioRxiv - Immunology 2020Quote: ... Radiolabeled protein was purified by size exclusion chromatography (PD-10 column, GE Healthcare, 17-0851-01) and injected in the lateral tail vein of tumor-bearing mice ...
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bioRxiv - Bioengineering 2021Quote: ... mAbs were captured from the clarified green juice using MabSelect SuRe Protein A columns (GE Healthcare). Protein A Columns were equilibrated and washed with buffer containing 50 mm Tris ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further purified using an AKTA FPLC system with HiTrap-Phenyl-HP column (GE Healthcare) and Superdex 200 size exclusion chromatography ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted protein was dialysed by anion-exchange chromatography using a 1ml ResourceQ column (GE Healthcare) equilibrated with 20 mM potassium HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... The collected supernatant was purified using a 1 ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5 ml kappa-select column (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... Ribosomes bound to an anti-HA antibody was purified using protein G magnetic beads (GE Healthcare). The RNA bound to ribosomes was purified with RNeasy Protect Mini Kit (QIAGEN ...
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bioRxiv - Cell Biology 2022Quote: ... The homogenates were precleared by incubation with protein G Sepharose (GE Healthcare, cat#17-0618-02) for 1 hour at 4°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were further purified by size exclusion chromatography using a Superdex 200 HR column (GE Healthcare) and eluted in 20 mM Tris-HCl pH 7.9 ...
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bioRxiv - Immunology 2022Quote: ... TAU-2212 in the supernatant was captured and purified by using protein A beads (GE healthcare), and was eluted by 0.1 M glycine at pH 3.2 ...
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bioRxiv - Immunology 2022Quote: ... the supernatants consisting of secreted antibodies were collected and trapped by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and concentrated using Centricon Plus-70 (051555 ...
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bioRxiv - Microbiology 2022Quote: ... The protein solution was then loaded onto an equilibrated 5 mL HiTrap-Q columns (GE Healthcare) using an ÄKTA pure 25 system (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: ... The eluted protein solution was then buffer exchanged using a PD-10 (GE Healthcare Life Sciences) desalting column.
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bioRxiv - Microbiology 2022Quote: ... The supernatants were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... The proteins were further purified by Superdex75 or Superdex200 gel-filtration column (GE Healthcare, Piscataway, NJ). For crystallization experiments ...
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bioRxiv - Biophysics 2019Quote: ... The protein was purified by IEX on a HiPrep Q FF anion exchange column (GE Healthcare) and then further purified by SEC on a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... pH 7.0) and loaded to a 1-ml HiTrap Protein G Sepharose FF column (GE Healthcare). The column was washed with binding buffer until the UV280 absorbance dropped to a stable baseline ...
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bioRxiv - Biochemistry 2019Quote: ... Plasma proteins were separated by anion exchange chromatography using Resource Q column (GE Healthcare, 6 ml) connected to an ÄKTA Avant 25 system (GE Healthcare) ...
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bioRxiv - Immunology 2019Quote: ... Proteins were transferred to a nitrocellulose membrane (Amersham Protran 0.45 µm NC, GE Healthcare, Buckinghamshire, UK) using a semi-dry Western Blot system ...
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bioRxiv - Synthetic Biology 2019Quote: ... This protein was further purified using a size exclusion column (HiLoad16/600 Superdex 200pg, GE Healthcare) equilibrated with TED containing 100 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... IP is carried out with clarified cell lysate pre-cleaned by protein G sepharose (GE Healthcare), then the cell lysate was incubated with anti-LRRC33 or control mouse IgG (2μg/ml ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were further purified using mono Q anion exchange chromatography and Superdex75 16/60 (GE Healthcare) size-exclusion chromatography ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant containing soluble proteins were collected and allowed to flow through GST4B resin (GE Healthcare). After washing with three column volumes of sonification buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The resulting protein was concentrated and purified by size-exclusion chromatography using Superdex 75 (GE Healthcare) with 10mM Tris pH8.0 ...
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bioRxiv - Genetics 2021Quote: ... Protein lysates were resolved by 12% SDS-PAGE and transferred to PVDF membrane (GE Healthcare AmershamTM) using a Trans-Blot® SD Semi-Dry Transfer Cell (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... pH 5.0 immediately before purification on a HiTrap Protein G HP 1 mL column (GE Healthcare) using an AKTA pure instrument ...
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bioRxiv - Microbiology 2021Quote: The CA proteins were loaded onto a HiLoad 16/60 Superdex 200 column (GE Healthcare, USA), and were eluted with CA loading buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was purified using a 5 mL HisTrap HP column (GE Life Sciences, now Cytiva), using wash [0.05 M Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were blotted on a 0.45 μM pore size nitrocellulose membrane (GE Healthcare, Chicago, IL, USA) using a Transblot SD semi-dry transfer system (Bio-rad ...
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bioRxiv - Immunology 2020Quote: ... mAbs were purified directly from culture supernatants using HiTrap protein G columns (GE Healthcare Life Sciences) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... MuSK1B was purified from culture supernatants using HiTrap Protein G High Performance column (GE Life Sciences) equilibrated in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... The secreted antibodies were subsequently purified from the supernatant on HiTrap Protein G columns (GE Healthcare). Plasmid DNA encoding a gliadin-reactive IGHV4-4:IGKV4-1 antibody with R in position 55 (corresponding to the IGHV4-4*07 allele ...
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bioRxiv - Systems Biology 2019Quote: ... The cleaved proteins were further purified on a 5 mL HiTrap Q HP column (GE Healthcare) and then a Superdex 200 10/300 GL size-exclusion column ...
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bioRxiv - Biophysics 2019Quote: ... the protein was subjected to separation on the Ni sepharose 6 Fast Flow resin (GE Healthcare) using the same buffers as above to separate the protein from the cleaved tag ...
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bioRxiv - Cell Biology 2021Quote: ... 20 µg of GST fusion protein and 20 µl of Glutathione Sepharose 4B Beads (GE Healthcare) were incubated with 1 ml of HeLa cell extract for 2 h at 4°C ...