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Citations for GE Life Sciences :
2451 - 2500 of 4095 citations for Cow Ataxin 10 ATXN10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the cleared supernatant was loaded onto a 10 ml Q Sepharose® HP column (GE Healthcare Europe GmbH, Freiburg, DE) equilibrated in buffer B ...
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bioRxiv - Microbiology 2022Quote: Size-exclusion chromatography (SEC) was employed to the Doderlin isolation in an ÄKTA purifier 10 (GE Healthcare, Chicago, Illinois, EUA) system ...
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bioRxiv - Immunology 2022Quote: ... Additional purification was then carried out by size-exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) with 2 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Both proteins were mixed in a molecular ratio 1:1 and the AR3C-HC84.1 Fab mixture was re-purified by SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare) with PBS as the eluent.
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with cold phenol red-free DMEM/10% FBS buffered by 20 mM HEPES (GE Healthcare Life Sciences) to remove unbound viruses ...
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bioRxiv - Biochemistry 2022Quote: Purified protein or protein complex (>1.5 mg/mL) was subject to a 24 mL Superdex 200 analytical gel filtrating column 10/300 GL (GE healthcare) at a rate of 0.5 mL/min at 20 °C by HPLC (Varian) ...
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bioRxiv - Bioengineering 2022Quote: ... the supernatant was filtered using 0.2 μm filters and buffer exchanged in storage buffer (20 mM Tris-HCl pH7.4, 200 mM NaCl and 5% glycerol) using PD-10 desalting columns (GE healthcare). The desalted sample then was aliquoted and stored at -80°C until the one-step purification/ immobilisation.
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... polypeptides were purified with size exclusion chromatography (SEC) column Superdex 200 10/300 increase (GE Healthcare, Little Chalfont, United Kingdom) which equilibrated with the SEC buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Developmental Biology 2021Quote: ... such that 500 µl was loaded onto on a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare). The gel filtration run was performed in sizing buffer containing 25 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... using a Superdex 200 Increase 10/300-Gl column with a flow rate of 0.3 ml/min on an Äkta purifier (GE Healthcare). Four milligrams of protein was loaded in a volume of 0.4 ml (8,7 mg/ml) ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA (1 – 10 μg) was separated using formaldehyde (2.2 M) gel electrophoresis and transferred to a Hybond-NX Nylon membrane (GE Healthcare) via capillary transfer ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mL imidazol gradient) or by size exclusion chromatography (SEC) using a Superdex® 75 10/300 GL (GE Healthcare) with a flow rate of 0.5 mL/min and dialysis buffer as running buffer ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare) in elution buffer (40 mM HEPES ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.4 and the sample was run over a Superdex 75 Increase 10/300 GL gel filtration column (GE Healthcare) equilibrated on the same buffer supplemented with or without 150 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... and the supernatant injected (using 1 ml injection loop) in a Superdex 75 10/300GL gel filtration column (GE Healthcare), previously equilibrated with 50mM Tris pH8 buffer ...
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bioRxiv - Microbiology 2020Quote: ... A 500-µL sample was subjected to size exclusion chromatography on a Superose 12 10/300 GL column (GE Healthcare) in buffer P using an ÄKTA pure (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Cell suspension was passed through a 40 μm plastic cell strainer and overlaid onto 10 mL Ficoll-Hypaque (GE Healthcare), followed by centrifugation (1000x g ...
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bioRxiv - Microbiology 2020Quote: ... Buffer exchange to a storage/reaction buffer (50 mM Tris, 300 mM NaCl, pH 8.0) was performed with a desalting column (PD-10, GE Healthcare) and the protein was transferred to a conical tube and diluted to 1 mg/mL as determined by absorbance at 280 nm for long-term storage ...
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bioRxiv - Microbiology 2020Quote: ... the flow-through was buffer exchanged to PBS and concentrated using Vivaspin filters (10 kDa molecular weight cutoff; GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... Complex formation was assayed by comparing the elution volumes in SEC on either a Superdex 200 Increase 10/300 or Superdex 200 Increase 3.2/300 (GE Healthcare) column ...
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bioRxiv - Biochemistry 2021Quote: ... After Bio-beads removal the mixture was centrifuged and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) column equilibrated with buffer 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... agitated for 30 min at room temperature before the buffer was changed to PBS (pH 7.4) with a PD-10 desalting column (GE Healthcare). Full-length D-serFS was then concentrated to 200-300 μM using centricons (Vivaspin 500 ...
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bioRxiv - Neuroscience 2020Quote: Protein samples concentrated to ∼1.0 g/L were injected into an HPLC-driven SEC column (Superdex 200 10/30 column, GE Healthcare) equilibrated with HBS-C buffer ...
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bioRxiv - Neuroscience 2020Quote: ... labelled protein samples were subjected to size exclusion chromatography at 4°C (Superdex 200 Increase 10/300 GL, GE Healthcare) in 50 mM phosphate (pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of purified sample (3.8 mg/mL TbMORN1(7-15) and 2.6 mg/ml TgMORN1(7-15)) were injected onto a Superdex 200 10/300 (GE Healthcare) column and the flow rate was set to 0.5 ml/min ...
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bioRxiv - Biophysics 2021Quote: ... ∼900 μL of 100 μM TS-β1AR was applied at 0.3 mL/min to a 24-mL Superdex 200 Increase 10/300 GL column (GE healthcare) pre-equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µL of sample (10 µl injection loop loaded with 50 µL sample) were injected on a Superdex 75 PC 3.2/30 (GE Healthcare) analytical size exclusion column preequilibrated with the protein buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 0.15 M (NH4)2SO4 pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...
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bioRxiv - Immunology 2022Quote: ... pooled plasma (n=14-15 per pool) was injected onto a Superose 6 HR 10/300 GL column (GE Healthcare) and eluted at a constant flow rate of 500 μL/min ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a Superdex 200 10/300 GL column using an ÄKTA chromatography system (GE Healthcare Life Sciences). 0.5 ml or 1 ml fractions were collected and analyzed by Western blotting using antibodies against HOIP and V5.
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bioRxiv - Biochemistry 2022Quote: ... ΔCter and free p85α were further subjected to gel filtration using SuperdexTM 200 10/300 GL Increase from GE healthcare. After gel filtration ...
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bioRxiv - Biochemistry 2022Quote: ... and then immobilized in 7 cm IPG strips (pH 3-10 : 70×3×0.5 mm) and a linear gradient (NL) (GE Healthcare Immobiline™ Dry Strip IPG ...
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bioRxiv - Biochemistry 2022Quote: ... and washed with 10 column volumes washing buffer (20 mM Tris, 500 mM NaCl, pH 7.8) using an AKTA explorer 100 (GE Healthcare). ARF-DBD proteins were eluted by 1 h incubation with 40 mM DTT in washing buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Samples collected from IMAC were exchanged into size exclusion chromatography (SEC) buffer (20 mM Na2HPO4, 150 mM NaCl, pH 7.4) using either a HiPrep 26/10 desalting column (GE Healthcare) or through multiple rounds of concentration and dilution using an Amicon Ultra-15 filter unit with a 10 kDa molecular weight cut-off ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing tagged-SbtB7001 were pooled and transferred into binding buffer using a HiPrep 26/10 Desalting column (GE Healthcare). The His6–Ub tags were removed using a ubiquitin carboxyl-terminal hydrolase 2 catalytic core domain (USP2cc):SbtB7001 molar ratio of 1:25 at 4 °C overnight as previously described [14] ...
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bioRxiv - Microbiology 2019Quote: ... Eluates were buffer exchanged into gel filtration buffer (25 mM Tris-Cl, 150 mM NaCl, pH 7.5) via HiPrep 26/10 desalting column (GE Healthcare), followed by overnight incubation with Ulp-1 to cleave off the His6-tagged SUMO moiety ...
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bioRxiv - Biochemistry 2019Quote: ... Elution was performed with the lysis buffer containing 250 mM imidazol pH 8.0 and eluted proteins were directly attached on a 10 ml Streptavidin Sepharose (GE Healthcare). Proteins were extensively washed with Strep buffer (20 mM Hepes ...
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bioRxiv - Biophysics 2019Quote: ... Size exclusion chromatography was carried out using Superdex S75 16/60 HiLoad and Superdex 75 10/300GL columns (GE Healthcare) with 20 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... 5-10 µg of proteins were resolved on 4–12% SDS-polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with various antibodies prior to development with ECL prime (GE Healthcare) ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were separated into 24 fractions using the 24 cm IPG strips pH 3-10 NL (GE HealthCare, Chicago, IL). To achieve the required low salt concentration 2.5 mg wheat germ extract was diluted with water and centrifuged 10 min. ...
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bioRxiv - Biochemistry 2019Quote: ... The assembly and purity of complexes from peak fractions of the Superdex200 10/300 GL gel filtration column (GE Healthcare) was assessed by SDS-PAGE gel electrophoresis and SimplyBlue SafeStain (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... The most concentrated IR-ECD-containing fraction was either desalted using disposable 8.3 ml Sephadex G-25 PD-10 desalting columns (GE Healthcare) equilibrated with HBS by centrifugation ...
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bioRxiv - Biochemistry 2019Quote: ... the concentrated protein was gel-filtrated using a Superdex 200 10/300 GL column equilibrated in HBS at a flow rate of 0.5 ml/min and 4 °C (GE Healthcare). Peak fractions at a final OD280nm of ∼ 0.4 (∼ 1.4 μM ...
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bioRxiv - Biophysics 2019Quote: Dimer hydrodynamic radii were compared by size-exclusion chromatography (SEC) using a Superdex 75 10/300 GL column (GE Healthcare) equilibrated with 1 M NaCl 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: Solid-phase synthesis of oligoribonucleotide was performed on a 1 μmol scale using AKTA Oligopilot plus 10 synthesizer (GE Healthcare) and Custom PrimerSupport™ Ribo A 40 (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... Base subcomplex was further purified by size-exclusion chromatography using a Superose™ 6 increase 10/300 column (GE Healthcare) pre-equilibrated with base GF buffer (60 mM HEPES pH 7.6 ...
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bioRxiv - Molecular Biology 2019Quote: ... FANCD2 and FANCI at 1 µM were sequentially run on a Superdex 200 10/300 column (GE Healthcare Life Sciences) equilibrated in 50 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Eluted proteins were further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Life Sciences). Purified proteins were concentrated by ultrafiltration prior to flash freezing with liquid nitrogen.