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Citations for GE Life Sciences :
2401 - 2450 of 2839 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Immune cells were isolated using a Ficoll gradient as described by the manufacturer (GE Healthcare, GE17-0891-01). The resulting immune cells were resuspended in 1x PBS prior to being plated in a 96-well plate for flow staining.
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells were isolated from buffy coats by density centrifugation using Ficoll-Paque Plus (GE Healthcare). CD14+ monocytes were subsequently isolated using magnetic-activated cell sorting (Miltenyi Biotech ...
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bioRxiv - Cancer Biology 2022Quote: ... Ptch;p53 primary cell-lines (#9410) were transfected with 40 nM ON-TARGETplus mouse Yap1 siRNA (GE Healthcare Dharmacon ...
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bioRxiv - Cell Biology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from the blood samples using Ficoll-Paque (GE Healthcare, Cat# 17144003) and gradient centrifugation ...
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bioRxiv - Immunology 2023Quote: ... Hepatic parenchymal cells were removed by density gradient centrifugation at 700 × g in 33.75% Percoll PLUS (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... The cell-free lysate was applied to a 5 ml His Trap™ HP column (GE Healthcare, USA) equilibrated in the same buffer ...
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bioRxiv - Cell Biology 2023Quote: ... cells were imaged using a 60x oil immersion objective lens on a DeltaVision deconvolution microscope (GE Healthcare, UK) equipped with an IX71 inverted microscope (Olympus ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... High-throughput imaging was carried out using the In Cell Analyzer 2000 with a 20X objective (GE Healthcare). Neuronal γH2AX signal was detected based on the overlap of γH2AX with TUBB3 ...
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bioRxiv - Molecular Biology 2023Quote: ... washed three times with PBS and then imaged in PBS with the IN Cell Analyzer 2200 (GE Healthcare) at 4x magnification ...
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bioRxiv - Biochemistry 2024Quote: ... Clear cell lysates (clarified by centrifugation) were purified with Ni-HiTrap FF and MBPtrap HP columns (GE Healthcare). Then tag-cleaved proteins was purified with ion exchange chromatography (HiTrap SP FF column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2024Quote: ... Mononuclear cells were extracted from bone marrow samples using Ficoll-Paque™ PLUS (GE Healthcare #17-1440-02), followed by red blood cell lysis using a red blood lysis buffer (Sigma #R7757).
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bioRxiv - Biochemistry 2024Quote: ... cells were imaged using a UPLSAPO 100x / 1.4NA oil objective on a DeltaVision deconvolution microscope (GE Healthcare, UK) equipped with an IX71 inverted microscope (Olympus ...
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bioRxiv - Microbiology 2024Quote: ... The infection was assessed by using an IN CELL Analyzer 6500 high-throughput automated confocal microscope (Ge Healthcare) located in BSL-3 safety laboratory ...
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bioRxiv - Cell Biology 2024Quote: Live-cell fluorescence imaging was performed using a Delta Vision Elite widefield fluorescence microscope (GE Healthcare Life Science) equipped with a 100× PlanAPO oil-immersion objective lens (Olympus ...
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bioRxiv - Cell Biology 2019Quote: ... Fluorescence images were acquired with an API DeltaVision Live-cell Imaging System (Applied Precision Inc., GE Healthcare Co., U.S.A.) and were captured at x60 magnification ...
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bioRxiv - Cell Biology 2019Quote: ... Fluorescence images were acquired with an API DeltaVision Live-cell Imaging System (Applied Precision Inc., GE Healthcare Co., U.S.A.) and were captured at x60 magnification ...
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bioRxiv - Cell Biology 2020Quote: ... cells were visualized live using the GFP or mCherry filter set of the DeltaVision Elite (GE Healthcare, Pittsburg, PA).
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bioRxiv - Immunology 2021Quote: ... single cell suspension of thymi were subjected to density-gradient centrifugation with a three-layer Percoll gradient (GE Healthcare) with gravities of 1.115 ...
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bioRxiv - Cell Biology 2020Quote: ... Fluorescence microscopy of live (time-lapse movies) and fixed cells were acquired using a Deltavision microscope system with softWoRx 4.1.2 software (Applied Precision), 100× 1.4 NA UPlanSApo oil immersion objective ...
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bioRxiv - Physiology 2022Quote: ... Buffy coat preparations of peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation over Ficoll-Paque Plus (GE Healthcare). Cell pellets of PBMCs were snap frozen prior to analysis for unprenylated proteins.
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bioRxiv - Molecular Biology 2020Quote: ... All GST-B3 fusion proteins were expressed in E.coli BL21(DE3) cells and were purified by gluthathione-Sepharose affinity chromatography according to the manufacturer’s recommendations (GE Healthcare). The proteins were eluted in elution buffer (20 mM reduced glutathione ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were recorded by acquiring one field of view per well in a 96-well black plate (GE healthcare). We used our previously developed semi-automated pipeline for segmentation and tracking of individual cells5.
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bioRxiv - Immunology 2019Quote: ... All His-tagged proteins were purified from the cell culture medium using HisTrap Excel column (GE Healthcare, catalog 17371206) and eluted using buffer containing 50 mM HEPES-NaOH ...
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bioRxiv - Genomics 2019Quote: ... for 10 minutes at room temperature and subsequently washed and imaged on an IN Cell Analyzer 2200 (GE Healthcare). Images were background-subtracted using FiJi (Schindelin et al. ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Clarified cell supernatants were concentrated 10-fold using Kicklab tangential flow filtration cassettes (GE Healthcare, cut-off 10 kDa) and adjusted to pH 8.0 in 10 mM Tris before purification in an AKTA Avant system by Steptrap-HP chromatography (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary CD8+ and CD4+ T cells were isolated from buffy coats form healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were cultured in DMEM (Nacalai, Osaka, Japan) supplemented with 10% fetal bovine serum (GE Healthcare, UT, USA) and a mixture of 100 units/ml penicillin and 100 μg/ml of streptomycin (PS ...
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bioRxiv - Neuroscience 2019Quote: ... cells were harvested and rAAVs were purified using 1 mL HiTrap heparin columns (GE Healthcare Bio-Sciences, Uppsala, SwedenSigma) and concentrated using Amicon Ultra centrifugal filter devices (Merck Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... The soluble fraction of the cell lysate was purified on HiTrap IMAC HP columns (GE Healthcare # 17-0920-05) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Time-lapse live cell imaging was carried out with the GE DV Elite Applied Precision DeltaVision system (GE Healthcare) equipped with Olympus oil objectives of 60X (NA 1.42 ...
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bioRxiv - Cancer Biology 2022Quote: ... peptides from whole cell samples were focused on immobilized pH gradient (IPG) gel strips (GE Healthcare, Waukesha, WI, USA) on a linear pH range of 3-10 ...
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bioRxiv - Biochemistry 2020Quote: ... Immunofluorescent intensities of H3K9Ac mark in the nuclei (total ~ 2500 – 4500 nuclei/treatment) were quantified by high-content image analysis (IN Cell Analyzer Workstation 3.7.2, GE Healthcare). H3K9 acetylation levels were reported after the intensities of the acetyl mark were normalized to DMSO-treated samples ...
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bioRxiv - Neuroscience 2020Quote: HEK293T and Neuro-2a cells were grown in DMEM (Biowest, L0104) supplemented with 10% FBS (GE Healthcare Hyclone, 12350273).
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bioRxiv - Microbiology 2021Quote: ... Primary CD8+ and CD4+ T cells were isolated from buffy coats form healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were purified from buffy coats by density gradient centrifugation (Ficoll-Paque Plus; GE Healthcare) and then cryopreserved in FBS with 10% DMSO ...
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bioRxiv - Neuroscience 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were enriched by density gradient separation using Ficoll (Ficoll-Paque™ plus, GE Healthcare). Monocytes were isolated from PBMCs with anti-CD14 conjugated magnetic microbeads (130-050-201 ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors by Ficoll-Hypaque density gradient centrifugation (GE healthcare). Monocytes were isolated from PBMCs using magnetic CD14+ isolation beads (Miltenyi) ...
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bioRxiv - Microbiology 2021Quote: ... Images were analyzed using the Multi Target Analysis Module of the In Cell Analyzer 1000 Workstation Software (GE Healthcare). GFP-positive cells were identified using the top hat segmentation method and counted within the InCell Workstation software.
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bioRxiv - Molecular Biology 2019Quote: ... Clarified cell lysate produced as for D2-I was loaded onto a HisTrap HP column (GE Healthcare Life Sciences). Using an imidazole gradient ...
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bioRxiv - Molecular Biology 2019Quote: ... Cell supernatants were collected using 10000 rpm for 10 min and incubated with Glutathione sepharose 4B resin (GE Healthcare) for 2 hr at 4°C ...
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bioRxiv - Immunology 2019Quote: ... the cells were resuspended in approximately 2 mL of 100% Percoll solution (Cat. no. 17-0891-01, GE Healthcare), and layered carefully on top of 3 mL of 80% and 40% Percoll solution ...
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bioRxiv - Cell Biology 2019Quote: Images of the extracellular vesicles and cells were taken with the DeltaVision wide field fluorescent microscope (Applied Precision, GE) outfitted with a digital camera (CoolSNAP HQ ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysate is incubated with 100ul of equilibrated IgG slurry (IgG SepharoseTM 6 Fast Flow from GE Healthcare) overnight in rotator wheel at 4°C with slow rotation ...
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bioRxiv - Microbiology 2019Quote: Cells extract were subjected to 10% SDS-PAGE and transferred to an Amersham Hybond™-P membrane (GE Healthcare). Membranes were incubated with an HIV-1 p24 monoclonal antibody diluted to 1/1000 (Catalog number 3537) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Media (DMEM, Glibco) supplemented with 10 % Fetal Bovine Serum (FBS, GE Healthcare), 1 % GlutaMAX (Glibco ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc fusion protein was purified from the cell culture supernatants with the protein A Sepharose (GE Healthcare). The purified ACE2-mouse IgG2a Fc fusion protein was biotinylated with Sulfo-NHS-LC-Biotin (Thermo) ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were lysed and recombinant KRAS G12D was purified using a HisTrap HP (GE Healthcare, 29-0510-21) column ...
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bioRxiv - Immunology 2021Quote: The purification of 2H1 from NS0 cells consisted of affinity chromatography using rProtein A/Protein G GraviTrap (GE Healthcare). The antibodies were eluted with a 0.1 M glycine-HCL pH 2.7 solution and immediately neutralized with a 1 M Tris-HCl 1 M NaCl pH 9.0 buffer to maintain their stability in solution ...
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bioRxiv - Immunology 2020Quote: ... Secreted Fabs were purified from cell supernatants harvested four days post-transfection using Ni2+-NTA affinity chromatography (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Immunology 2020Quote: ... and purified from clarified transfected cell supernatants four days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Affinity-purified proteins were concentrated and further purified by size-exclusion chromatography (SEC ...