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Citations for GE Life Sciences :
2201 - 2250 of 2758 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated using the density gradient centrifugation method (GE Healthcare, STEMCELL Technology). Briefly ...
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bioRxiv - Immunology 2022Quote: ... The immune cells were obtained by density gradient centrifugation with Percoll (17-0891-09, GE Healthcare, USA) according to the manufacture’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... and the peripheral blood mononuclear cells (PBMCs) isolated by Ficoll-Paque gradient (GE Healthcare, Boston, MA, USA). Informed consent was obtained in accordance with the Helsinki Declaration and the Review Board of the Dana-Farber Cancer Institute.
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bioRxiv - Cell Biology 2022Quote: ... cells were spotted onto slides and images were acquired using a Deltavision Elite microscope (GE Healthcare Biosciences) with a 60X oil immersion objective ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant of cell lysate was purified over Glutathione-Sepharose 4B (GS4B) (GE Healthcare, Boston, MA, USA) or Ni-NTA resins (Qiagen) ...
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bioRxiv - Pathology 2020Quote: ... We also prepared red blood cells from EDTA-treated swine blood by using Percoll (GE Healthcare, USA) and kept it in an RPMI 1640 medium (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation (Ficoll-Hypaque, GE Healthcare life sciences) from buffy coats from healthy donors (Sanquin Amsterdam ...
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bioRxiv - Molecular Biology 2019Quote: Primary CD4+ T cells were isolated from buffy coats from healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCells Technologies) ...
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bioRxiv - Microbiology 2021Quote: Cell lysates were separated using 8-12% polyacrylamide gels and transferred to Amersham Nitrocellulose Membranes (GE healthcare) via Trans-blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... hematopoietic cells were enriched by density gradient centrifugation with 40/90 Percoll gradient (GE Healthcare Life Sciences) for 30 min at 2,500 rpm ...
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bioRxiv - Genomics 2019Quote: ... Mononuclear cells (MNCs) were isolated by density gradient separation (Ficol-Paque, GE Healthcare Life Sciences, Pittsburgh, PA) and frozen for later use ...
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bioRxiv - Cell Biology 2020Quote: We performed the quantitative evaluation of surface area of cardiomyocytes by using IN Cell Analyzer (GE healthcare). αActinin positive cardiomyocytes with nuclei (Hoechst ...
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bioRxiv - Biochemistry 2020Quote: ... Sf9 insect cells were infected with baculovirus in Hyclone CCM3 media (GE Healthcare Life Sciences, Pittsburgh, PA) for 48 hrs ...
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bioRxiv - Immunology 2020Quote: ... The resuspended cells were subjected to lymphocyte enrichment using Ficoll-Paque Plus (17-1440-02, GE Healthcare). The decidual leukocytes were further purified by fluorescent activated cell sorting with 7-AAD (420404 ...
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bioRxiv - Biophysics 2020Quote: ... and the clarified cell extract was loaded onto a 5-mL HisTrap HP column (GE Life Sciences) equilibrated in HisTrap Buffer A ...
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bioRxiv - Bioengineering 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh human peripheral blood using Ficoll (GE Healthcare) density gradient centrifugation ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors using Ficoll-Paque PLUS (GE Healthcare). CD4 T cells were purified from PBMCs using anti-human CD4+ magnetic beads (Miltenyi ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was isolated from the cells using an Illumina RNAspin Mini RNA isolation kit (GE Healthcare). The samples were treated with DNase I to remove any contaminating genomic DNA ...
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bioRxiv - Immunology 2020Quote: ... The soluble fraction was purified from the cell lysate using Glutathione Sepharose 4B beads (GS4B, GE Healthcare). The proteins were then digested with PreScission protease (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from whole blood using Ficoll-Paque-PLUS (GE Healthcare 17144002). CD14+ monocytes were purified using MojoSort™ Human CD14 Nanobeads (Biolegend 480093 ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was purified from cleared cell lysate using Ni-sepharose 6 Fast flow resin (GE Healthcare) in a poly-prep column (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were injected onto a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences). To slow Fe2+-oxidation and precipitation in equilibration buffer ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was isolated from the cells using an Illumina RNAspin Mini RNA isolation kit (GE Healthcare). The samples were treated with DNase I to remove any contaminating genomic DNA ...
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood by Ficoll-Paque (GE Healthcare, ThermoFisher) density gradient centrifugation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... MIN6 cells were grown in optimized DMEM (AddexBioTechnologies) supplemented with 10% FBS (HyClone, GE Healthcare Life Sciences), 50 μM 2-β-mercaptoethanol (Gibco ...
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bioRxiv - Biophysics 2022Quote: ... The dialyzed protein solution (∼50 μM) was then loaded into the sample cell in iTC200 (GE Healthcare) after the removal of insoluble materials by centrifugation (10,000×g ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SE001 was purified from cell supernatants after 5 days of induction using Nickel Excel resin (GE Healthcare), followed by size exclusion chromatography with a Sephadex S200 column (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells were isolated from whole blood samples using Ficoll-paqueTM (GE Healthcare Life Sciences), with subsequent RNA and DNA isolation as detailed in Vazquez Bernat et al ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was removed by resuspending pelleted cells into 30% isotonic Percoll (GE Healthcare, Cat#17-5445-02) and centrifuging at 300g for 15 minutes at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... A single-use 10 L Cell bag™ bioreactor (working volume: 0.5-5 L; GE Healthcare, USA) was placed on the rocker ...
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bioRxiv - Microbiology 2022Quote: ... cell culture media were clarified by centrifugation and the IgG captured using Protein A resin (GE Healthcare). Proteins were eluted from the resin using citrate buffer pH 3 ...
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bioRxiv - Microbiology 2023Quote: Cell lysates were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Amersham Protan, GE Healthcare). Incubation with specific antibodies was followed by peroxidase-conjugated secondary antibodies and detection using SignalFire ECL Reagent (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2023Quote: HMC3 cells were grown in EMEM media (ATCC) supplemented with 10% of bovine calf serum (GE healthcare) and 1x of Antibiotic and Antimycotic (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were removed and cell pellets were re-suspended in 37% isotonic Percoll (GE Healthcare, IL, USA) at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and the cell pellet was suspended and treated with a 37% Percoll (17089101, GE Healthcare Life Sciences) density gradient to enrich lymphocytes for flow cytometry assays ...
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bioRxiv - Cell Biology 2023Quote: NDC80-yeGFP expressing cells were imaged at room temperature using a DeltaVision Elite (eDV) microscope (Applied Precision) equipped with a PCO Edge5.5 sCMOS camera and Olympus 60X oil-immersion objective lens (Plan Apo N ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified cell lysate was applied to a nickel affinity column (GE Healthcare; Sepharose high performance beads) equilibrated in Buffer A and purified by FPLC ...
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bioRxiv - Neuroscience 2023Quote: Live imaging of GFP immunofluorescence in hCOs was obtained using an IN Cell Analyzer 2200 (GE Healthcare) at 4x or 10x magnification.
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bioRxiv - Neuroscience 2023Quote: ... coli and after cell disruption the cleared lysate was applied to a GSTrap chromatography column (GE Healthcare). After the on-column cleavage with 3C protease (prepared according to in house protocol) ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA from cell samples was extracted using the Illustra RNAspin kit with DNaseI treatment (GE Healthcare). RT-PCR was performed with 100 ng of total RNA using SuperScript III One-Step RT-PCR System (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... cell lysate was circulated over a 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) charged CoCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Red blood cells were precipitated by Ficoll sedimentation according to manufacturer’s instructions (Ficoll Paque Plus, GE Healthcare). Remaining cells were washed with HBSS (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... Cells were mounted on a slide and imaged using DeltaVision Elite wide-field fluorescence microscope (GE Healthcare) with a 60x/1.516 oil immersion objective ...
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bioRxiv - Cell Biology 2023Quote: All live cell microscopy was performed on a DeltaVision Elite Deconvolution microscope (Applied Precision, Issaquah, WA, USA). Directly before imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) were freshly isolated from heparinized blood using Ficoll-Paque Plus (GE Healthcare) density gradient centrifugation.
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bioRxiv - Molecular Biology 2023Quote: ... Live-cell imaging was performed on a Deltavision Elite system using a 40× oil objective (GE Healthcare). Images were acquired every 10 or 15 min for 48 h ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was added to equilibrated glutathione-Sepharose 4B beads (GE Healthcare; 1 ml/100 g cells) and gently rotated for 4 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: All live cell microscopy was performed on a DeltaVision Elite Deconvolution microscope (Applied Precision, Issaquah, WA, USA). Imaging pads were stained with 8 µL of dye mix (25 µg/mL DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... All other cell lines were grown in Dulbecco’s Modified Eagle’s Medium (GE Healthcare Life Sciences, Logan, UT) with 10% fetal bovine serum (10% FBS–DMEM) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified from soluble fractions of the bacterial cell lysates using Glutathione Sepharose beads (GE Healthcare). Proteins were dialyzed in 1x TBS overnight at 4°C overnight and protein quantified ...