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Citations for GE Life Sciences :
2551 - 2600 of 2839 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Liver infiltrating cells were obtained after 25 min centrifugation (600 g without brake) in a 35% and 67.5% bilayer Percoll (GE Healthcare) gradient ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation on Ficoll-Paque Plus (GE Healthcare, Boston, MA, USA). CD3+ T cells from PBMCs were separated using CD3 microbeads (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2023Quote: RNA was isolated from plated cells or snap-frozen kidneys using the RNAspin Mini kit (GE Healthcare, #25-0500-72) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Images of mouse spermatocyte spreads testicular cell squashes were acquired using a computer-assisted fluorescence microscope system (DeltaVision; Applied Precision) with a 60ξ NA 1.4 oil immersion objective ...
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bioRxiv - Immunology 2023Quote: ... Lymphocytes were isolated from the cell suspension using a 37 % and 70 % percoll gradient (GE Healthcare Bio-Sciences AB, Sweden) diluted with HBSS (with or without phenol red ...
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bioRxiv - Molecular Biology 2023Quote: The supernatant or cell extract was then filtered through a 0.45 µm pore filter and loaded on a HisTrap HP column (GE Healthcare) with immobilized Ni2+ beads equilibrated with HisBufferA (50 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized blood samples by separation on a Ficoll-Hypaque gradient (GE Healthcare). For detailed splenocyte isolation protocol ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells were isolated from screened buffy coats by density centrifugation with Ficoll-Plaque Plus (GE Healthcare) followed by magnetic-assisted cell sorting ...
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bioRxiv - Biophysics 2023Quote: YidC and YidCΔC were purified from pTrc99a-YidC (Welte et al., 2012) and pTrc99a-YidCΔC expressing BL21 cells using a Ni-NTA FF crude column (GE Healthcare) on an ÄKTA chromatography system ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then imaged at 4X using the InCell 2200 or 6500 automated fluorescence microscope system (GE Healthcare Life Sciences) and quantitative analysis was performed with Cell Profiler Software(182,183) ...
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bioRxiv - Immunology 2023Quote: ... from which the debris and the dead cells were removed by density gradient centrifugation with a 46% Percoll (GE Healthcare) gradient ...
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bioRxiv - Genomics 2023Quote: ... HepG2 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Hyclone, GE healthcare) and 1% Penicillin-Streptomycin (PS ...
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bioRxiv - Bioengineering 2024Quote: ... the soluble fraction of the cell lysate was passed through a 0.45-micron filter and then loaded onto a HisTrap HP 5 ml (GE Healthcare) column equilibrated with buffer A (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Systems Biology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of healthy donors by Ficoll-Paque (GE Healthcare, 17-1440-02) density gradient centrifugation ...
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bioRxiv - Cell Biology 2020Quote: Images were analyzed automatically by the IN Cell Developer Toolbox software (cat. 25-8098-26, GE Healthcare Bio-Sciences, Marlborough, MA). Nuclei of living and dead cells were identified by using the corresponding fluorescent signal of Hoescht33342 ...
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bioRxiv - Cell Biology 2020Quote: Proteins (30 to 50μg of total cell lysates) were separated in SDS-PAGE gels and transferred to PVDF membranes (Amersham Hybond-P, GE Healthcare). Membranes were then blocked in PBS-T (PBS – 0.1% Tween 20 ...
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bioRxiv - Immunology 2020Quote: ... CR2-Fc and FH19-20 were prepared in 10 mM sodium acetate pH 5 and immobilised at 300 RU (CR2-Fc: 20 µM, FH19-20: 240 µM) to different flow cells of CM5 chips (GE Healthcare) using standard amine coupling involving preparation of the dextran matrix with 100 mM N-hydroxysuccinimide (NHS ...
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bioRxiv - Molecular Biology 2022Quote: Hela and Hela-RNAseH1 cells downregulated for the indicated genes were grown in the presence of 10 μM bromodeoxyuridine (BrdU, GE Healthcare) for 24 hours ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... After two centrifugation steps at 3000 x g to remove cell debris the AAV was isolated from the supernatant using heparin columns (GE Healthcare). After loading the column with the virus particles and washing with buffers containing 20 mM Tris and increasing concentrations of NaCl (100 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... and harvested from cell culture medium followed by purification procedure using Ni-NTA column and Superdex 200 gel filtration column (GE Healthcare) in tandem ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were incubated with standard erythrocyte lysis buffer on ice and separated from myelin and debris by Percoll gradient (GE Healthcare) centrifugation ...
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bioRxiv - Systems Biology 2019Quote: Time-lapse fluorescent microscopy for U2OS-Dual cells was performed at the biomolecular screening facility (BSF, EPFL) using an InCell Analyzer 2200 (GE healthcare). Experiments were performed at different temperature (34°C ...
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bioRxiv - Immunology 2019Quote: ... The brain tissues were quickly dissected and single cell suspensions were prepared and separated using a 37%/70% Percoll gradient (#GE17-0891-01, GE Healthcare). Myelin was removed from the top layer and mononuclear cells were isolated from the interface ...
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bioRxiv - Bioengineering 2019Quote: Cell-free supernatants were prepared by centrifugation and directly loaded (100 mL) onto a 5 mL HiTrap MabSelect column (GE Healthcare). After washing the column with binding buffer (20 mM sodium phosphate ...
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bioRxiv - Genomics 2019Quote: ZHBTc4 YPet-MD TIR1-V5 mCherry-OCT4-AID cells were plated in a 96-well plate coated for 1 hour at 37°C with 1:25 diluted StemAdhere (Primorigen Biosciences #S2071-500UG) and imaged on an IN Cell Analyzer 2200 (GE Healthcare) using the TexasRed and Brightfield channels ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: Total extract of CHO cells expressing the β1His6 construct were immobilized with nickel-nitrilotriacetic acid beads (Ni+-NTA, His Trap FF column; GE Healthcare) equilibrated with 10 ml of RIPA buffer containing protease inhibitors ...
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bioRxiv - Microbiology 2019Quote: ... After cell lysis by sonication cell debris was removed by centrifugation and the lysate was passed through a His-Trap column (GE Healthcare). Proteins were eluted with a gradient of buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: The recombinant catalytic domains of MMP-2 and -9 were expressed in HEK293 cells stably transfected with respective constructs and purified from serum-free culture medium using Gelatin Sepharose 4B (GE Healthcare) as described in [35 ...
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bioRxiv - Molecular Biology 2021Quote: ... The full-length human eRF3a was expressed in insect Sf21 cells and purified via affinity chromatography using a HisTrap HP column (GE Healthcare) followed by anion-exchange chromatography on a MonoQ column (GE Healthcare ...
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bioRxiv - Genetics 2020Quote: ... Whole cell lysates were quantified and 50 μg were resolved in 4-20% SDS-PAGE and transferred onto nitrocellulose membranes (GE healthcare). The membranes were then incubated with the primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... These cells were isolated from human blood by density-based centrifugation following layering of the blood onto Ficoll-Hypaque plus (GE healthcare). Cells were isolated and washed three times in RPMI (Gibco ...
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bioRxiv - Biochemistry 2020Quote: IgGs were produced by co-transfection of the heavy chain and light chain pEU vectors (76) in mammalian cells and the IgG1 variants were purified by protein A affinity chromatography (MabSelect Sure columns, GE healthcare) using the AKTA express system and eluted with 0.1 M sodium citrate (pH 3.0) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell pellets were resuspended in 4 ml PBS and subject to density gradient centrifugation with Ficoll-Paque PLUS (17144002, GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... connected to a ÄKTA Pure apparatus equipped with a UV 280 nm flow cell (all from GE Healthcare, Chicago, IL, USA). EV fractions were collected based on UV absorbance ...
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bioRxiv - Molecular Biology 2020Quote: ... After cell sonication proteins were affinity purified using a Glutathione sepharose 4b or remains on sepharose for pull down (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: His-tagged SARS VHH-72 or MERS VHH-55 was immobilized to a single flow cell of an NTA sensorchip at a level of ∼400 response units (RUs) per cycle using a Biacore X100 (GE Healthcare). The chip was doubly regenerated using 0.35 M EDTA and 0.1 M NaOH followed by 0.5 mM NiCl2 ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-human Fc monoclonal antibody R10Z8E9 was immobilised onto active and reference flow cells of a Series S sensor CM5 chip using an amine capture kit (both from GE Healthcare). Approximately 1000 response units of Pf34 bait protein was captured onto the active flow cell ...
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bioRxiv - Microbiology 2020Quote: dHepaRG or PHH cells seeded into a 24-well plate were transfected with 25 nM or 10 nM of siRNAusing Dharmafect#1 (GE HealthCare) or Lipofectamine RNAiMax (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... were diluted with PBS and layered with 35 mL of diluted cell suspension over 15 mL of Ficoll-Paque (GE healthcare) in 50 mL conical tube ...
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bioRxiv - Cell Biology 2021Quote: ... were collected by crushing of bones (tibias, femurs, hips, humeri and spine) and subjecting the cells to density gradient centrifugation (Ficoll-Paque Plus, GE Healthcare) at 400g for 25 minutes with brakes turned off ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK-293T (ATCC CRL-3216) cell lines used in this study were cultured at 37°C in 5% CO2 in high-glucose DMEM (GE Healthcare) supplemented with 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Biophysics 2020Quote: Recombinant N-terminal His6-tagged TRF1 was purified using a baculovirus/insect cell expression system and an AKTA Explorer FPLC (GE Healthcare) as described in a previous study (66) ...
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bioRxiv - Cell Biology 2020Quote: ... Fusion proteins were recovered from the cell lysates by conventional affinity chromatography on His-trapTMHP columns (GE Healthcare, 17-5247-01) using an Akta Explorer system (Amersham Pharm BiotechGE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... cell supernatants/lysates were precleared using 30 μl of a 50% slurry of Protein G Sepharose (GE Healthcare, #17-0618-01), while for IP of pyo-KSR1 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants of the cell lysates were run on 12% resolving Tris-glycine gels and then transferred onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). To detect PstP-strep-tag II ...
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bioRxiv - Biophysics 2022Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was expressed using Hi5 cells and purified by Ni-NTA resin and gel filtration chromatography (GE Healthcare) in HBS buffer (10 mM HEPES ...
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bioRxiv - Physiology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from age- and sex-matched mice by centrifugation over Ficoll-Paque Plus (GE Healthcare). Splenocytes were obtained by crushing the tissue through a 70μm nylon filter (Falcon) ...
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bioRxiv - Bioengineering 2022Quote: Human adult peripheral blood mononuclear cells (PBMCs) were purified by density centrifugation using Ficoll-Paque (density = 1.077 g/mL; 600g, 30 minutes; GE Healthcare; USA). Informed written consent was given by donors to give approval for the use of waste material for research purposes ...
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bioRxiv - Microbiology 2022Quote: ... An equal protein concentration or equal volume of cell and EV lysate was run in an SDS 10% polyacrylamide gel and subsequently transferred onto a nitrocellulose membrane (GE Healthcare). Total protein was measured by Ponceau S stain ...