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Citations for GE Life Sciences :
2251 - 2300 of 2758 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... All other cell lines were grown in Dulbecco’s Modified Eagle’s Medium (GE Healthcare Life Sciences, Logan, UT) with 10% fetal bovine serum (10% FBS–DMEM) ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were washed for 5 min three times with TBS-T before incubating at room temperature for 1 h with the secondary antibody (anti-rabbit HRP conjugated anti-rabbit IgG [GE Healthcare] diluted 1:5,000 in 3% BSA/ TBS-T). After washing the membranes three times in TBS-T buffer ...
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bioRxiv - Biophysics 2021Quote: ... cell debris was removed and the supernatant loaded onto a 10 ml volume nickel-sepharose column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Cell Biology 2020Quote: ... were added at 25ul/ml to exclude immune and endothelial cells using MagRack 6 (GE Healthcare 89129-096). Cells were either frozen for future use or stained for flow cytometry ...
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bioRxiv - Cell Biology 2020Quote: An IN Cell Analyzer 6000 automated fluorescence microscope (cat. 29-0433-23, GE Healthcare Bio-Sciences, Marlborough, MA) was used for imaging cells ...
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bioRxiv - Cell Biology 2020Quote: ... Live-cell imaging were performed using a DeltaVision RT wide-field fluorescence imaging system (Applied Precision, Issaquah, WA) equipped with a quantifiable laser module ...
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bioRxiv - Genetics 2021Quote: ... and RNA was extracted from cells using a magnetic bead-based protocol using Sera-Mag SpeedBeadsTM (GE Healthcare). Two libraries were prepared per clone using SMARTseqv4 kit (Clonetech) ...
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bioRxiv - Immunology 2022Quote: ... IgG proteins were expressed in transiently-transfected Expi293F cells and purified by protein A affinity chromatography (GE Healthcare) followed by SEC as described21,47.
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bioRxiv - Cell Biology 2022Quote: ... and 3T3 cells were cultured in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum (GE Healthcare), 100 U/ml penicillin and streptomycin ...
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bioRxiv - Cell Biology 2022Quote: Images for immunolabelled cells on micropatterns were acquired with an inverted wide field Deltavision Core Microscope (Applied Precision) equipped with highly sensitive cooled interlined charge-coupled device (CCD ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21 cells were grown at 37 °C with 5% CO2 in DMEM (HyClone, GE Healthcare life sciences) supplemented with 10% bovine serum and 1 U/mL penicillin/streptomycin ...
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bioRxiv - Immunology 2019Quote: ... Cells were strained through a 70 μm filter and separated on a discontinuous 40%/80% Percoll (GE Healthcare) gradient ...
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bioRxiv - Biochemistry 2019Quote: ... Cell-free supernatant was applied to the column at 2 mL/min using an AKTA Pure (GE Healthcare), washed ...
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bioRxiv - Biochemistry 2019Quote: ... Enzymes were purified from the cell lysate using a 1 ml His-Trap FF crude column (GE Healthcare) using an elution gradient from 10 to 250 mM imidazole in 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2019Quote: ... fixed and subjected to high content microscopy in a IN Cell Analyzer 6000(GE healthcare life sciences, USA).
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bioRxiv - Neuroscience 2019Quote: ... Mononuclear cells were isolated from CB and PB by density centrifugation using standard Ficoll-Hypaque technique (GE Healthcare) then treated with 0.15M NH4Cl to lyse residual erythrocytes and washed in phosphate-buffered saline (PBS).
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bioRxiv - Genetics 2020Quote: ... Fluorescently labeled glial cells were scored visually across optical stacks with a Deltavision Core imaging system (Applied Precision) and a PlanApo 60x/1.42 NA oil immersion objective.
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bioRxiv - Genomics 2020Quote: ... RNA was extracted from cells using a magnetic bead-based protocol using Sera-Mag SpeedBeads™ (GE Healthcare). Isolated RNA was DNase-treated with RQ1 DNase (Promega) ...
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bioRxiv - Physiology 2021Quote: ... The stained myotubes were imaged at 10–20X magnification on the IN Cell Analyzer 6000 platform (GE Healthcare). For each treatment condition ...
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bioRxiv - Cancer Biology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh buffy coats using Ficoll-Paque Plus (GE Healthcare) gradient centrifugation ...
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bioRxiv - Cancer Biology 2020Quote: ... Mononuclear cells (MNCs) were isolated by density gradient separation (Ficoll-Paque PREMIUM; GE Healthcare, Little Chalfont, Buckinghamshire, UK) and immediately analyzed or vitally frozen for later use ...
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bioRxiv - Cell Biology 2021Quote: ... Gene-edited NIH3T3 cells were cultured at 37°C in 5% CO2 in DMEM (GE Healthcare, Chicago, IL) containing 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were resuspended in 40% Percoll solution and overlayed on 70% Percoll (#17-0891-01, GE Healthcare). After a density centrifugation step (at 2100 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... the cell lysates were directly subjected to size exclusion chromatography using an ÄKTA purifier (GE Healthcare Life Science). The column was washed with three column volumes (75 ml ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 cells as described previously.13 The protein was purified using glutathione-Sepharose 4B beads (GE Healthcare) concentrated and flash-frozen in GST buffer [25 mM Tris (pH 7.5) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then the cell suspensions were loaded on 1.5 % agarose pads and analyzed by DeltaVision Elite microscope (Applied precision).
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Paque Plus gradient separation (GE Healthcare, Chicago, IL, USA). Cells were stored in 10% dimethyl sulfoxide in fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: 35S-positive bands within NP40 cell extracts were detected in a Typhoon system (GE Healthcare Life Sciences, UK). The autoradiography protein profile observed in the non-supplemented or Cu-supplemented cells was represented as a heatmap of blot intensity (ranging white to blue ...
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bioRxiv - Cell Biology 2020Quote: Image stacks of cells were collected at 0.3 μm z increments on a DeltaVision Elite workstation (Applied Precision) based on an inverted microscope (IX-70 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant from the centrifuged cell lysate was loaded onto a Q Sepharose fast flow column (GE Healthcare). In vitro nitrogenase activity was determined by monitoring acetylene reduction to ethylene as previously described.14 Ethylene and acetylene were quantified using gas chromatography (activated alumina 60/90 mesh column ...
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bioRxiv - Cancer Biology 2022Quote: ... The culture supernatant of the retrovirus-producing cells was passed through a 0.45 mm filter (Whatman, GE Healthcare) and immediately used for infection ...
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bioRxiv - Immunology 2022Quote: ... Total CD4+ T cells were collected from buffy coats by first performing a Ficoll-Paque Plus (GE Healthcare) density gradient centrifugation to isolate peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells (PBMCs) from COVID-19 patients were isolated from the blood using Ficoll (GE Healthcare) density gradient media and transferred into 96 well plates at a concentration of 2.5 × 106 viable cells per ml in 200µl (0.5 × 106 cells per well ...
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bioRxiv - Microbiology 2022Quote: ... Neutrophils were purified from the remaining cells by overlaying on a Ficoll density gradient (GE Healthcare, Chicago, IL) following centrifugation in Ficoll-Paque Centrifugation Media (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and mononuclear cells isolated by density gradient centrifugation on a discontinuous 35%/70% Percoll (GE Healthcare) gradient (2000 rpm [931 g] ...
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bioRxiv - Biophysics 2022Quote: ... These cells were cultured in RPMI 1640 with 2.05mM L-glutamine (GE Healthcare Bio-Sciences Corp., MA, USA) supplemented by 5% v/v fetal bovine serum (FBS ...
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bioRxiv - Genomics 2020Quote: ... The main difference between the Cellomics platform and the follow up IN Cell Analyzer 6000 system (GE Healthcare) is the eased use in automated C1 array scans and the capability of the IN Cell Analyzer to take z-stacked images ...
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bioRxiv - Molecular Biology 2020Quote: ... Sf9 cells were maintained in Hyclone CCM3 (CCM3 liquid medium with L-glutamine, GE Healthcare Life Sciences SH30065.02) supplemented with 50 U/ml Penicillin-Streptomycin (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Images were acquired and analyzed using the automated microscope IN Cell 2000 Analyzer (GE Healthcare, Little Chalfont, UK). Images of six randomized fields per well having ∼ 600 cells each were acquired ...
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bioRxiv - Immunology 2021Quote: Blood was collected using EDTA-tubes and cells isolated using Ficoll-Paque Density Centrifugation (GE Life Sciences, Canada). Bronchoalveolar lavage (BAL ...
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bioRxiv - Immunology 2022Quote: ... Images acquired by the IN Cell Analyzer 2000 were analysed using the Developer Toolbox software V1.9.2 (GE Healthcare). Data are shown as representative images or the percentage overlap of OTII T cells with DCs.
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bioRxiv - Immunology 2019Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated using a Ficoll-Paque Plus 1440 gradient (GE Healthcare, Uppsala/Sweden), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... His6–Ub-tagged SbtB7001 was purified from cell lysate using a 5 ml HisTrap FF column (GE Healthcare): the pre-equilibrated and lysate-loaded column was washed with binding buffer (20 mM NaH2PO4 ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified cell lysate was passed through a HiTrap SP HP cation exchange chromatography column (GE Healthcare Life Sciences) to remove impurities ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The stained myotubes were imaged at 10–20X magnification on the IN Cell Analyzer 6000 platform (GE Healthcare). For each treatment condition ...
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bioRxiv - Molecular Biology 2019Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood using Ficoll– Paque density gradient centrifugation (GE Healthcare). CD14+ cell isolation was done using immunomagnetic bead selection according to manufacturer’s instructions to achieve 95–99% purity (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins from the cleared cell lysate were captured in a HisTrap HP 5 ml column (GE Healthcare) in His buffers (20 mM MOPS ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown at 37°C and 5% CO2 in high-glucose (4.5 g/L) DMEM (GE Healthcare) supplemented with 10% fetal calf serum (Biochrom ...
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bioRxiv - Molecular Biology 2020Quote: Sf9 cells were maintained in suspension cultures in serum-and antibiotic-free HyClone CCM3 media (GE Life Sciences) at 27°C and 125 rpm ...
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bioRxiv - Synthetic Biology 2021Quote: ... The cell lysate was clarified by centrifugation and loaded onto a HisTrap FF column (GE Healthcare, Piscataway, NJ) that had been pre-equilibrated with 10 column volumes of binding buffer (50 mM sodium phosphate ...