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Citations for GE Life Sciences :
2651 - 2700 of 2839 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Neutrophils (purity > 97%, viability > 98%) were isolated from healthy heparinised peripheral blood using HetaSep (Stem Cell) and Ficoll-Paque (GE Healthcare), and contaminating erythrocytes were removed by hypotonic lysis as described previously (Wright et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins in cell lysates were separated on SDS-PAGE and then transferred electrophoretically onto PVDF membranes (GE Healthcare, Milwaukee, WI, USA) using a transfer cell (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... Protein was purified from filtered cell culture supernatants by StrepTactin resin (IBA) and by size exclusion chromatography using Superose 6 column (GE Healthcare) in 10 mM Tris pH8,150 mM NaCl or 2 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: The distance between two GFP foci on chromosome IV on a single focal plane in a intact yeast cell taken by the fluorescence microscope system (Delta vision, Applied Precision) was measured by NIH image J program.
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bioRxiv - Cell Biology 2022Quote: ... cells were washed and incubated with DAPI and anti-bleach reagents prior to imaging on a DeltaVision Elite Microscope (GE Healthcare). For all experiments ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates from 8 L of yeast treated cells were then subjected to a 5 ml Nickel affinity purification using an AKTA purifier system (GE Healthcare) equilibrated in the urea buffer ...
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bioRxiv - Immunology 2022Quote: ... Umbilical cord blood mononuclear cells (UCBMC) and plasma were obtained by standard density gradient centrifugation over Ficoll (GE Healthcare, Chicago, IL). UCBMC were frozen in 10% DMSO/FetalPlex (GeminiBio ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed and cleared lysate prepared as described and loaded onto a 5 mL Strep-Trap HP column (GE Healthcare), equilibrated with buffer D ...
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bioRxiv - Plant Biology 2023Quote: ... The ASK1-D3/D3D720K (D3 or D3 with D720K mutation) complex was isolated from the soluble cell lysate by Q Sepharose High Performance resin (GE healthcare). 600 mM NaCl eluates were further subjected to Nickel Sepharose Fast Flow (GE healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... 60 μl of this mixture was added in the HUVEC side channel of the chip and max intensity projections were taken every 5 minutes for 30 minutes using the IN Cell Analyzer 6000 (GE Healthcare) microscope.
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bioRxiv - Biophysics 2022Quote: ... Broken cells then underwent ultra-centrifugation at 142,000 x g for 60 minutes before collecting the cell free extract and incubating it with pre-equilibrated HisTrap Ni2+ resin (GE Healthcare) in a 3:1 volume ratio for 2 hours under gentle shaking ...
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bioRxiv - Cancer Biology 2022Quote: ... U87vIII CHCHD2 WT and KO cells were seeded onto collagen-coated dextran beads (∼200 µm diameter, GE Life Sciences, Pittsburgh, PA) at a density of 2 × 106 cells per 5 × 103 beads in 5 mL DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated with secondary antibodies conjugated to different fluorophores (1:750 dilution) and were imaged in the laser-based InCell high-content imaging system (IN Cell Analyzer 6000, GE Healthcare). The nuclear levels of S9.6 or the number of γ-H2AX foci was quantified using the INCarta software that is attached to the imaging system ...
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bioRxiv - Immunology 2024Quote: ... LP cells were further purified by gradient centrifugation at 2800 rpm for 20min with 44% and 67% Percoll (GE Healthcare/Cytiva) at room-temperature ...
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bioRxiv - Genomics 2024Quote: ... WSC3-e cells (1.6 µg protein µL-1) was loaded onto a Superdex 200 10/300 column (GE Healthcare, Chicago, USA), which was equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.84) ...
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bioRxiv - Immunology 2024Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy blood donors (provided by the Stockholm blood bank) through Ficoll-Hypaque (GE Healthcare) density-gradient centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were obtained 24 hours after treatment and GST was purified on a 1 mL GSTrap HP column (GE Healthcare) with an AKTA purifier 10 FPLC system ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation using a Ficoll-Paque Plus gradient (GE Healthcare, Cat #17-1440-02), then washed by centrifugation ...
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bioRxiv - Biophysics 2022Quote: ... All BtuF constructs were purified directly from whole cell lysate (obtained by sonication) via Ni-NTA chromatography followed by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) in final buffers of 20-50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... was immobilised to approximately 2100 response units (RU) on a flow cell of a Streptavidin coated (SA) sensor chip (GE healthcare) according to manufacturer’s directions while another flow cell was left blank for reference subtraction and monitoring any non-specific bindings ...
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bioRxiv - Biochemistry 2023Quote: ... DAP5 was first purified from bacterial cell lysate using a 5 mL His-Trap HP (Ni-NTA) column from GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... rPF4 was purified from soluble fractions of cell lysates by affinity chromatography using a 5-ml HiTrap heparin-agarose column (GE Healthcare). Endotoxin-free rPF4 was prepared as previously described 3 ...
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bioRxiv - Cell Biology 2023Quote: ... Time-lapse imaging of one-cell embryos expressing CYB-1::mNG or mNG::CYB-3 was performed on a widefield deconvolution microscope (DeltaVision Elite; Applied Precision) equipped with a charge-coupled device camera (pco.edge 5.5 sCMOS ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies were expressed by transient transfection in Expi-CHO cells as murine IgG2a Fc domain conjugates and purified using the HiTrap MabSelect SuRe (GE Healthcare) affinity chromatography ...
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bioRxiv - Neuroscience 2023Quote: ... Erythroblasts were isolated when reaching 60-70% of the total cell population by gradient centrifugation at 1000 x g for 20 minutes at room temperature over Percoll (GE Healthcare). Isolated erythroblasts were frozen in FBS containing 10% DMSO at -80°C ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Microbiology 2023Quote: ... unpurified proteins contained in the supernatant of transfected HEK293F cells were immobilized on 2G12 or PGT145 functionalized CNBr-activated Sepharose 4B column (GE Healthcare) by flowing at 1 mL/min or overnight incubation at 4 °C on a roller bank ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were centrifuged at 4°C to remove cell debris and the supernatant was filtered and loaded onto a Nickel Sepharose Fast Flow column (GE Healthcare) in the case of pSY10 constructs ...
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bioRxiv - Microbiology 2023Quote: ... Mononuclear cells were collected and washed with saline to remove the platelets and then separated by centrifugation through a Percoll (GE Healthcare) gradient (40.48% and 47.70%) ...
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bioRxiv - Biochemistry 2023Quote: Recombined cells expressing either GFP-WT Parkin or GFP-R42P Parkin variant were seeded into a 96-well plate and imaged the next day using the InCell2200 cell imaging system (GE Healthcare). Fluorescence microscopy was performed using the excitation and emission filter settings of DAPI (excitation ...
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bioRxiv - Cell Biology 2022Quote: ... SUMO-tagged and GST-tagged TEAD4-YBD were expressed in Escherichia coli BL21 (DE3) cells and purified using the Ni2+-NTA agarose resin (GE Healthcare) or GST agarose resin (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: GST fusion protein was similarly purified using affinity chromatography by incubating bacterial cell lysate with glutathione-Sepharose 4B beads (#17075601; GE Healthcare) according to the manufacturer’s instructions and eluted from beads with elution buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... All BtuF constructs were purified directly from whole cell lysate (obtained by sonication) via Ni-NTA chromatography followed by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) in final buffers of 20-50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2022Quote: Primary human AML specimens and normal MPB samples were resuspended at about 100-200 e6 cells per ml in freezing media composed of 50% FBS (GE Healthcare), 10% DMSO (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... Biomass concentrations were monitored using the cell growth quantifier (Scientific Bioprocessing) and determined manually by measuring the optical density at 600 nm with an Ultrospec 10 Cell Density Meter (GE Healthcare). The conversion factor of OD600 to cell dry weight (CDW ...
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bioRxiv - Cell Biology 2023Quote: ... insoluble material was removed by centrifugation at 10,000 × g and the cell lysate was pre- cleared by addition of heat inactivated goat serum and protein G sepharose beads (GE Healthcare). Proteins were then either immunoprecipitated by adding 2 μg mouse anti-GFP (Clones 7.1 and 13.1 ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was extracted and from cell monolayers using a magnetic bead-based protocol using Sera-Mag SpeedBeads™ (GE Healthcare). Isolated RNA was DNase-treated with RQ1 DNase (Promega) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant of the cell lysate was incubated overnight at 4°C on a wheel with Glutathione Sepharose 4B resin (GSTrap 4B, GE Healthcare). After several washes ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was stained with DAPI (1 µg/mL) for 5 minutes at room temperature and cells imaged with a DeltaVision Core system (Applied Precision).
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bioRxiv - Molecular Biology 2023Quote: ... and imaged on a Nikon Eclipse Ti2 at 60X or 100X objective with oil immersion or at 20X using In Cell Analyzer 6000 microscope (GE Healthcare). Images were captured and analyzed using Elements software (v 5.41.02) ...
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bioRxiv - Biochemistry 2024Quote: ... The pooled fraction was concentrated on an Amicon 10kDa MWCO cell and applied to a sephacryl S300 HR column (GE Healthcare) equilibrated with Buffer F (25mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... DSS-treated cell lysates with ultracentrifugation and DSS-treated AcGFP were injected onto a Superdex 200 Increase (10/300GL) column (GE Healthcare), using the UltiMate 3000 UHPLC system (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) from fresh peripheral blood of healthy donors were isolated by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare). Cytotoxic T cells and T helper cells were isolated from the PBMCs by positive selection with CD8- and CD4-specific MicroBeads (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2024Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from heparin-treated whole blood by density gradient centrifugation (Ficoll-Paque PREMIUM, GE Healthcare). After separation ...
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bioRxiv - Microbiology 2023Quote: ... from 3 different healthy donors to avoid unique donor bias on type cells effects and extracted using Ficoll-Plaque Plus solution (GE Healthcare). PBMCs were re-suspended in RPMI-1640 medium (Lonza Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... Gp140 proteins were purified from clarified cell culture supernatants 4 days post-transfection using Galanthus nivalis lectin (GNL) affinity chromatography followed by a Q-sepharose (GE Healthcare) polishing step to remove host cell protein contaminants ...
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bioRxiv - Microbiology 2024Quote: Peripheral blood mononuclear cells (PBMCs) were recovered from heparinized blood via density gradient centrifugation using Ficoll-Plaque separation media (GE Healthcare) and Leucosep tubes (Greiner Bio-One) ...
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bioRxiv - Neuroscience 2024Quote: Quantitative analysis of the sagittal sections was performed using the automated microscope IN Cell Analyzer 6000 (GE Healthcare, Chicago, IL, USA). Four microscope slides were loaded into the system ...