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Citations for GE Life Sciences :
2301 - 2350 of 6651 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were clarified followed by protein A affinity chromatography with MabSelect SuRe LX protein A resin (GE Healthcare) using the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... 6His–GST–CRK2cyto (WT, K353E and D450N) were expressed in Escherichia coli Lemo21 and purified by Glutathion Sepharose 4B (GE Healthcare, Chicago, USA). 6His–MBP–PLDα1 and 6His–MBP–CALS1_N were expressed in E ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM UltraGlutamine (GE Healthcare, UK), BME vitamins (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2 mM 2-mercaptoethanol for 12 h and purified by anion exchange chromatography (HiTrap Q, GE Healthcare) with a linear gradient of 0 to 500 mM NaCl over 40 min and size-exclusion chromatography (HiLoad 16/600 Superdex 200 ...
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bioRxiv - Microbiology 2020Quote: ... proteins were immobilized to a protein A sensorchip a level of ~500 response units (RUs) using Biacore 8000 (GE Healthcare) and a running buffer of composed of 20mM PB7.4 with 300 mM NaCl ...
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bioRxiv - Biophysics 2019Quote: The proteins and protein complexes were analyzed by analytical gel filtration using a Superdex 200 5/150 column (GE Healthcare) and the 1260 Infinity Bio-inert high-performance liquid chromatography system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences). The eluted fractions were collected for SDS-PAGE analysis.
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was further purified by fast protein liquid chromatography (FPLC) using a Source-S cation-exchange column (GE Healthcare) (low salt buffer with 50 mM potassium phosphate at pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were further purified by gel filtration on a fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principle peaks were collected ...
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bioRxiv - Zoology 2021Quote: ... Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare) as per manufacturer’s instructions (Figure A1 ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was added to 50 µl G-Protein Sepharose beads (Protein G-Sepharose 4 Fast Flow, GE Healthcare) (pre-washed with the lysis buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein G-coupled sepharose (#17–0618–01) and Protein A-coupled sepharose (#17–5138–01) beads were from GE Healthcare and the protease inhibitor cocktail was from Sigma.
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected with protein-specific primary antibodies and HRP-coupled secondary antibodies by enhanced chemiluminescence (GE Healthcare) and imaged using X-ray films or with Fusion Capture Advance FX7 16.15 (Peqlab).
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred onto a Hybond P 0.45 polyvinylidene difluoride (PVDF) membrane (GE life sciences) using wet blotting ...
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bioRxiv - Cell Biology 2020Quote: ... The gel was subsequently electroblotted onto a nylon membrane (Amersham Hybond N+, GE Healthcare) as described 55 ...
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bioRxiv - Cell Biology 2020Quote: ... and membranes were imaged on an ImageQuant LAS4000 imaging system (GE Healthcare Life Sciences). Dot intensity data is in Supp Table S2.
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bioRxiv - Genetics 2021Quote: ... and electrotransferred to 0.2 μm pore size nitrocellulose membranes (Amersham Protran Supported, GE Healthcare). The membranes were blocked for 1 hour at room temperature using 5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were visualized by phosphorimaging on a Typhoon FLA 9500 phosphorimager (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The complexes were transferred to an Amersham Hybond-N+ nylon membrane (RPN303B, GE Healthcare), UV-crosslinked ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked in prime blocking agent (GE Healthcare, Cat#RPN418, Chicago, IL, USA), then incubated with primary Phospho-P70S6K (Thr389 ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 M urea gel and transferred onto an Amersham Hybond-XL membrane (GE Healthcare) for Northern Blotting analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA was blotted to an Amersham Hybond-NX membrane (GE Healthcare Life Sciences) followed by UV-crosslinking (254 nm) ...
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bioRxiv - Molecular Biology 2021Quote: ... we transferred the RNAs from the gel to HybondTM-N+ nylon membrane (GE Healthcare) by capillary transfer O/N and cross-linked the RNA to the membrane by an ultraviolet cross-linker (120 mJ/cm2 at 254 nm ...
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bioRxiv - Microbiology 2021Quote: ... The RNA was afterwards blotted onto Nylon membranes (Amersham Hybond-N+, GE Healthcare, RPN203B) with 10X saline-sodium citrate buffer (SSC ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were separated on a 10% gel and transferred onto nitrocellulose membrane (GE Healthcare). Anti-β-tubulin (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA was transferred overnight to Hybond-N+ nylon membrane (GE Healthcare, Chicago, Illinois, RPN303B) by capillary action in transfer buffer (0.5 M NaOH ...
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bioRxiv - Cell Biology 2021Quote: ... and then the membranes were scanned using Image Quant LAS4010 (GE Healthcare Life Sciences). We obtained the primary antibodies for ERK (#9102) ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was exposed and scanned using the Typhoon FLA 9500 Phosphoimager (GE Healthcare) and analyzed using the QuantityOne (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Separated RNA was electro-transferred from the gel to Hybond-N+ membranes (GE Healthcare) at 50 V for 1 h at 4°C and the membranes were UV-crosslinked (120 mJ/cm2) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were developed using ECL Prime (Amersham) and ImageQuant™ LAS 4000 (GE Healthcare). Proteins were visualized with SyproRuby (BioRad ...
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bioRxiv - Immunology 2021Quote: ... and transferred by semi-dry technic onto Amersham Hybond-P PVDF membranes (GE Healthcare). After blocking ...
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bioRxiv - Microbiology 2021Quote: ... membrane was finally incubated with HRP-conjugated anti-mouse IgG (1:5000; GE Healthcare) overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... gels were electrophoretically transferred (200mA for 1hr) onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). After blocking (blocking solution ...
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bioRxiv - Microbiology 2020Quote: ... and transferred on a 0.1 µm Amersham Protran nitrocellulose membrane (GE Healthcare Cat #10600000) using a Pierce G2 Fast Blotter ...
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bioRxiv - Cell Biology 2020Quote: ... and electrotransferred onto 0.2-µm-pore-size nitrocellulose membranes (Amersham Protran Supported, GE Healthcare) using either XCell SureLock Mini-Cell with XCell II Blot Module or Mini Gel Tank with Blot Module (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... The membranes were visualized with ECL select Western Blotting Detection Reagent (GE Healthcare, RPN2235) on ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT and applied to a prewetted nitrocellulose membrane (GE Healthcare, 0.2 µm) under vacuum using a Dot Blot Apparatus (SCIE-PLAS) ...
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bioRxiv - Molecular Biology 2022Quote: ... the membrane was submerged in the ECL Plus Western Blotting Detection Reagents (GE Healthcare) for 2 min and then subjected to the imaging system (UVITEC Cambridge ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was scanned using chemiluminescence settings on an Amersham Imager 600 (GE Healthcare).
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bioRxiv - Developmental Biology 2022Quote: ... and transferred at 300 mAmps for 42 minutes to a nitrocellulose membrane (GE Healthcare). The blot was probed with 4 1:5000 anti-KIF23 antibody.
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bioRxiv - Microbiology 2021Quote: ... Cell supernatant was diafiltrated over a 30 kDa membrane on a Quixstand (GE healthcare) to Tris/NaCl buffer (50 mM Tris/500 mM NaCl at pH 8.0) ...
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bioRxiv - Molecular Biology 2020Quote: ... Gels were wet-transferred onto nitrocellulose membranes (GE Healthcare Life Sciences, Cat. No 10600002) in ice-cold 1X Transfer Buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... chilled directly on ice and spotted on a nylon membrane (GE Healthcare Hybond-N+). After UV-induced fixation of target nucleic acids (2 x 200 000 μJ/cm2 of UV) ...
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bioRxiv - Microbiology 2021Quote: ... membranes were incubated in 1:10000 diluted anti-rabbit IgG (NA934-1ML; GE Healthcare) in TBS-T for one hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Membrane imaging was carried out using an ImageQuant LAS4010 Imager (GE Healthcare Life Sciences). Densitometric analysis was performed with ImageQuant TL (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2022Quote: ... the membranes were incubated with an anti-rabbit peroxidase-conjugated secondary antibody (GE healthcare). Chemiluminescence was obtained by the ICL Substrate (GE healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... samples were transferred to Hybond™ nitrocellulose membrane (GE Healthcare Life Sciences, Buckinghamshire, UK) using a wet system (Mini Trans-Blot Cell ...