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Citations for GE Life Sciences :
2551 - 2600 of 6651 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed and visualized with an enhanced chemiluminescence (ECL) detection system (GE Healthcare) and iBright CL1000 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... and then transferred to a PVDF membrane (0.45 µm pore size, Amersham Hybond, GE Healthcare) via electro-blotting ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were incubated with HRP-coupled secondary antibodies and revealed by chemiluminescence (GE Healthcare). MiniBAR-GFP stable cell lines transfected with siRNAs were directly collected in 1x loading buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed three times in TBST before bands were visualised using ECL (GE Healthcare), using the Chemidoc gel imaging system (Biorad) ...
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bioRxiv - Microbiology 2024Quote: ... separated by SDS-PAGE on 8% acrylamide gels and transferred onto nitrocellulose membrane (GE healthcare). Membranes were stained with Revert™ 700 total protein stain (LI-COR ...
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bioRxiv - Cancer Biology 2024Quote: ... were separated on gradient polyacrylamide gels and transferred to nitrocellulose blotting membrane (0.45uM; GE healthcare Primary antibodies against phospho mTOR (Cell signaling) ...
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bioRxiv - Microbiology 2024Quote: ... resolved on a 12% SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were incubated with 1:100 of the primary mouse antibodies ...
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bioRxiv - Molecular Biology 2024Quote: ... Membrane signals were developed and quantified using the ImageQuant ™ TL 8.1 software (GE Healthcare Life Sciences ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were imaged in an ImageQuant 800 luminescent system (GE Healthcare Life Sciences, Piscataway, NJ). Rubisco loading control was stained using Ponceau S (Sigma-Aldrich).
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bioRxiv - Physiology 2019Quote: ... Equal loading of protein content was verified using alpha-tubulin as a housekeeping protein and bands visualised using ECL detection system (GE Healthcare, UK). Bands were measured using Image J densitometry ...
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bioRxiv - Cell Biology 2021Quote: ... Postnuclear supernatants were incubated with 3 μg of antibodies coupled to protein A– or protein G–Sepharose beads (GE Healthcare, Solingen, Germany) for 4 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted with an imidazole gradient and fractions containing the target protein collected using an AKTA Prime system (GE Life Sciences). Protein quality was analysed by Coomassie gel analysis ...
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bioRxiv - Microbiology 2019Quote: ... Protein was then purified using the AKTA protein purification system with a Ni-NTA HisTrap affinity column (GE Healthcare GE17-5248-02), followed by passage through a Superdex 75 column for size exclusion chromatography ...
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bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA) following purification using HiTrap Protein-A column (GE healthcare, UK). The purified protein was sterile-filtered and stored in PBS.
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: Non-specifically binding proteins were cleared from each sample by incubation with 60 µL of 20% Protein A-Sepharose™ CL-4B (GE Healthcare) in IP Buffer (150 mM NaCl / 1% Triton X-100 / 15 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
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bioRxiv - Molecular Biology 2020Quote: ... proteins were visualized using ECL detection reagents (GE Healthcare). Uncropped gel images for western blots are available in Supplementary information 3.
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were bound on HisTrap (HP)(GE Healthcare) and eluted with increasing imidazole concentration ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein A-Sepharose 4 Fast Flow suspension (GE Healthcare) was washed with Tris-Buffered Saline (TBS ...
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bioRxiv - Immunology 2020Quote: ... using protein A affinity chromatography (GE Healthcare, Marlborough, MA). IgG from pooled samples that were collected from the macaques before immunization was purified as the control for passive antibody transfer ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein G beads from GE Healthcare (GE Healthcare, 10280243) were used to purify the target protein and then the protein sample was analyzed using western blot ...
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bioRxiv - Genomics 2020Quote: ... Protein G Sepharose beads (GE Healthcare, 4 Fast Flow) were washed with WB140 ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein was bound to glutathione affinity resin (GE Healthcare), washed with 50 mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μL of Protein G-sepharose beads (GE Healthcare) coated with 12 µL rabbit anti-Bicc1 ...
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bioRxiv - Cancer Biology 2019Quote: ... using a ÄKTA Purifier protein purification system (GE Healthcare). His-tagged proteins were eluted with a buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2020Quote: ... on an ÄKTA FPLC protein purification system (GE Healthcare) was used to further purify the Nbs and ensure their monomeric states ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were incubated with dithiothreitol (DTT -GE Healthcare) at 10 mM and 30°C for one hour ...
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bioRxiv - Biophysics 2021Quote: ... in an AKTA go protein purification system (GE Healthcare). The purified FRET constructs were divided into 200 μL aliquots ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins transferred onto nitrocellulose (GE Healthcare, Chicago, IL) or polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µl samples of Protein A-Sepharose (GE Healthcare) were preincubated with 10 µl each of purified α-MyoE or α-Uso1 antibodies for 3 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... and Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads by dimethyl pimelimidate cross-linking as described in (69).
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bioRxiv - Plant Biology 2021Quote: ... dilution) together with protein A-Sepharose beads (GE Healthcare) were used ...
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bioRxiv - Biophysics 2020Quote: ... and purified by protein A affinity chromatography (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... the protein was purified with Source-Q (GE Healthcare) and Sephacryl S100 columns (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare). The panning process was described in detail in our previous protocol [64].
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by ECL reagent (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... and by HRP-conjugated protein A (GE Healthcare, NA9120V). Colorimetric reaction was started by adding BM Blue POD substrate (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were incubated with Protein A Sepharose (GE Healthcare) for 1-2 hr at 4°C and bound proteins were washed extensively with lysis buffer and eluted with SDS sample buffer with boiling.
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bioRxiv - Genomics 2022Quote: ... were bound to protein A magnetic beads (GE Healthcare) and then incubated with MNase extracts over night at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein was bound to glutathione affinity resin (GE Healthcare), eluted by cleavage of the GST-tag with TEV ...
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bioRxiv - Biochemistry 2019Quote: ... PreScission proteinase (8 units/mg of protein, GE Healthcare) was added to remove the His6 tag from the Asf1 ...
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bioRxiv - Cell Biology 2020Quote: ... the protein was purified on Heparin column (GE Healthcare), eluted by NaCl gradient elution and dialyzed for 2 days (20 mM phosphate buffer ...
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bioRxiv - Immunology 2019Quote: ... with Protein-G Sepharose (GE Healthcare, Milwaukee, WI, USA).
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bioRxiv - Immunology 2020Quote: ... and purified through protein A affinity chromatography (GE Healthcare), followed by size exclusion chromatography (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were measured using BCA assay (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Synthetic Biology 2021Quote: ... were bound to protein A magnetic beads (GE Healthcare) and then incubated with MNase extracts over night at 4°C ...
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bioRxiv - Immunology 2020Quote: ... with Protein-G Sepharose (GE Healthcare, Milwaukee, WI, USA).
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bioRxiv - Biochemistry 2021Quote: ... on an ÄKTA START protein purification system (GE Healthcare). The sample was loaded in a buffer containing 20 mM phosphate buffer saline (PBS ...
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bioRxiv - Biophysics 2019Quote: ... using the ÄKTA pure protein purification system (GE Healthcare). The final complex was diluted in Storage buffer with 50% glycerol ...
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bioRxiv - Microbiology 2019Quote: ... in an ÄKTA Star protein purification system (GE Healthcare) following the manufacturer’s instructions ...