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Citations for GE Life Sciences :
2451 - 2500 of 6651 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at - 80°C until use ...
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bioRxiv - Biophysics 2019Quote: ... After dilution of the protein sample to a DTT concentration of 1 mM the reduced protein were immobilized on a Ni2+-Sepharose resin (GE Healthcare) and washed with ten column volumes of buffer A (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at 16,000xg for 10 min and the clarified lysates were pre-adsorbed to Protein A or Protein G beads (GE Healthcare Bioscience) for 2 hr at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Elution fractions containing the untagged protein were pooled and the homogenous protein was finally isolated by size-exclusion chromatography onto a Superdex 75 column (GE Healthcare) equilibrated in 20 mM Hepes/NaOH ...
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bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The antibody was purified from culture supernatant by protein G affinity chromatography using the ÄKTA Pure system with a HiTrap Protein G column (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
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bioRxiv - Cell Biology 2019Quote: ... 2) A Deltavision Elite System (GE Healthcare) equipped with an IX-71 inverted microscope (Olympus) ...
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bioRxiv - Immunology 2019Quote: ... 20U/ml Xuri IL-2 (GE Healthcare) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... with 2% cosmic calf serum (GE Healthcare), 25 ng/mL EGF (R&D ...
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bioRxiv - Molecular Biology 2020Quote: RNAs were electro-transferred from urea-PAGE gels to Hybond XL nylon membrane (GE Healthcare) at 12 V over 12 h in 0.5X TBE for Northern hybridisation ...
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bioRxiv - Microbiology 2021Quote: ... DNA fragments were transferred to a nylon membrane (Hybond™-N+) (GE Healthcare, Dornstadt, Germany) by capillary blotting ...
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bioRxiv - Molecular Biology 2021Quote: ... membranes were scanned on an Image Quant LAS 4000 (GE Healthcare, Little Chalfont, Buckinghamshire, UK) imaging station.
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bioRxiv - Neuroscience 2022Quote: ... 20% MetOH) to a nitrocellulose membrane (Amersham™ Protran™ 0,45μm NC, GE Healthcare Lifescience). Quality of transfer was checked by Ponceau-S (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed in TBST before using the ECL Western Blotting Detection Reagent (GE Healthcare). The chemiluminescence signal was detected using the Amersham Imager 600 (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... Pieces of the retina were flattened on a filter membrane (Whatman, GE Healthcare Life Sciences) and placed on a poly-L-lysine (0.1% ...
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bioRxiv - Molecular Biology 2020Quote: ... gDNA on the gel was vacuum transferred onto a hybond nylon membrane (GE Healthcare GERPN303B) with 10XSSC (1.5M NaCl 0.17M NaCitrate ...
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bioRxiv - Molecular Biology 2019Quote: ... then loaded onto a cellulose acetate membrane with a 0.2 µm pore size (GE Healthcare) that had been incubated in 1% SDS in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were visualized using the chemiluminescent substrate of an ECL kit (GE Healthcare – RPN2232). Images were captured on ImageQuant LAS 4000 (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... and supernatants resolved by SDS-PAGE and transferred to Hybond-ECL nitrocellulose membrane (GE healthcare). The membrane was blocked with 5 % nonfat dry milk in PBS-T ...
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bioRxiv - Neuroscience 2019Quote: ... were transferred to nitrocellulose membranes and processed for immunoblotting using ECL Prime kit (GE Healthcare). Images of immunoblots were captured using ChemiDoc (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNA was separated on formaldehyde gels and then blotted on Nytran membranes (GE Healthcare). Following crosslinking and methylene blue staining (SERVA) ...
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bioRxiv - Cell Biology 2019Quote: ... resolved by 8% SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare, Munich, Germany) by semi-dry blotting ...
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bioRxiv - Molecular Biology 2019Quote: ... Each 75 μL-sample was spotted on a nylon membrane (Amersham Hybond-XL; GE Healthcare) by vacuum filtration (PR648-HoeferTM Slot Blot) ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was developed using enhanced chemiluminescence (ECL Prime Western Blotting detection reagent, GE Healthcare) in an ImageQuant LAS 4000 (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction mix was then transferred to the positively charged nylon transfer membrane (GE Healthcare) and washed extensively with 50 mM sodium phosphate buffer ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... pH 7.5 and filtered through a 0.22µm polyether-sulfone membrane (GE Healthcare, Chicago, IL, USA) to remove any aggregates ...
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bioRxiv - Neuroscience 2021Quote: ... and resolved by denaturing polyacrylamide electrophoresis before electrophoretic transfer to Nytran Plus membranes (GE Healthcare) and hybridization with [32P]-end labeled oligonucleotide probes at 42°C or direct staining ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was washed and incubated with Amersham ECL Western Blotting Detection Reagent (GE Healthcare) and developed in the dark ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was incubated with a secondary anti-Rabbit horseradish peroxidase conjugated antibody (GE Healthcare) for 45 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... transferred to nitrocellulose membranes and radioactivity was detected in a Typhoon FLA950 apparatus (GE Healthcare). Later ...
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bioRxiv - Plant Biology 2020Quote: The blot was semi-dry transferred to Amersham Hybond™-N+ nylon membrane (GE Healthcare) and cross-linked under 0.12 J/cm2 UV irradiation ...
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bioRxiv - Neuroscience 2020Quote: ... containing a cellulose acetate membrane with pores with a 0.2 μm diameter (Whatman, GE Healthcare) as described in (Torres et al. ...
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bioRxiv - Plant Biology 2021Quote: ... gels were bolted onto a positively charged nylon membrane (Amersham, now GE Healthcare, http://www.gelifesciences.com). The DNA was linked using a UV light cross-linker instrument equipped with 254-nm bulbs for 0.8 min exposure.
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bioRxiv - Genomics 2021Quote: ... Electro-transfer was performed in TBE overnight onto a Hybond N+ membrane (GE Healthcare, RPN303B). Gene-specific probes were generated by random priming in the presence of ATP [α32P] using the Prime-It II Random Primer Labeling Kit (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... Fixed VLPs were filtered onto 0.02 μm filter membranes in triplicate (Anodisc, GE Healthcare, USA) and were stained with SYBR-Gold (2.5X concentration) ...
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bioRxiv - Molecular Biology 2020Quote: ... Solubilized membranes were then bound to a 5-ml Ni-NTA column (HisTrap, GE Healthcare), which was subsequently washed with 50 mM Tris pH 8 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were incubated with streptavidin conjugated to horse radish peroxidase (1:5000) (GE Healthcare, RPN1231VS) in 1% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... the antibody-bound membranes were treated with enhanced chemiluminescent western blot detection reagents (GE Healthcare) and visualized with an x-ray film (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... subjected to polyacrylamide gel electrophoresis (PAGE) and electro-transferred onto nitrocellulose membranes (GE Healthcare, Germany). Blocking with 5% non-fat dry milk or bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were subsequently developed using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare #RPN2236). Images were acquired through the ChemiDoc MP Imaging System ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were incubated with peroxidase-conjugated secondary antibodies at 1: 5000 dilution (GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Physiology 2022Quote: ... membranes were incubated with an ECL Western Blotting Detection reagent (catalog no. RPN2106, GE Healthcare). Acquisition of the chemiluminescent signal was done using X-Ray films (catalog no ...
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bioRxiv - Cancer Biology 2022Quote: ... Following electrophoretic separation we perfomed transfer blotting on nitrocellulose membranes (Amersham Protran, GE Healthcare, USA); which were subsequently incubated overnight at 4 °C with primary antibodies as follows ...
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bioRxiv - Plant Biology 2021Quote: ... the membrane was treated with ECL Select western blotting detection reagent (GE healthcare, Chicago, IL) and signal was detected with Licor Odyssey imaging system (LI-COR Bioscience ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with TBS-T and scanned on a chemiluminescence imager IA600 (GE Healthcare). For the KNL1 pMELT immunoblots ...
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bioRxiv - Neuroscience 2021Quote: ... the supernatant containing the viral particles was filtered using 0.45 PVDF membrane filters (GE Healthcare). The viral purification was performed using OptiPrep solution (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... Membranes were washed three times with 1X TBST and incubated with ECL (45002401, GE Healthcare) for 5 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... and this membrane was covered by one piece of Whatman filter paper (GE Healthcare, 1002055). The filter unit was placed on a glass vacuum flask (Fisherbrand ...
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bioRxiv - Developmental Biology 2019Quote: ... membranes were developed and analysed using the ImageQuant LAS400mini and the ImageQuantTL Software (GE Healthcare). The antibodies used are listed in Table S6.