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Citations for GE Life Sciences :
2101 - 2150 of 6458 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... except that antibody-bound protein complexes were isolated with protein A sepharose beads (GE Healthcare Life Sciences) washed with RIPA buffer (140mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... pGEX-4T-2 (GE Healthcare), pGIPZ control shRNA ...
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bioRxiv - Plant Biology 2021Quote: ... 2 M thiourea (GE Healthcare), 1% DTT ...
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bioRxiv - Plant Biology 2020Quote: ... pGEX6p-2 (GE healthcare, IL) and pBE2113-GFP (Yoshida et al. ...
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bioRxiv - Cell Biology 2023Quote: ... 2% horse serum (GE Healthcare), 0.5% penicillin-streptomycin (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was centrifuged at 9,400× g at 4 °C for 20 min and the supernatant was applied onto a 5 mL His-Trap TALON crude column (GE Healthcare Bioscience, Piscataway, NJ, USA) pre-equilibrated with deionized water and Talon binding buffer ...
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bioRxiv - Cell Biology 2021Quote: ... bacterial-expressed GST and GST-α-Taxilin-M (expessed in Escherichia coli strain BL21) were bound to glutathione-Sepharose 4B beads (GE Healthcare) and incubated with bacterial-expressed MBP-γ-Taxilin-M or 293T cell expressed and purified 3×FLAG-CEP170 at 4 °C overnight ...
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bioRxiv - Biophysics 2022Quote: ... coli upec-117 CapW at 5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in buffer GF ...
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bioRxiv - Immunology 2022Quote: ... IgG proteins were expressed in transiently-transfected Expi293F cells and purified by protein A affinity chromatography (GE Healthcare) followed by SEC as described21,47.
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bioRxiv - Biochemistry 2019Quote: ... Recombinant proteins were routinely purified by fast protein liquid chromatography (FPLC) using an AKTA purifier system (GE Healthcare), eluting with a linear gradient of 25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... 200 pmoles of purified proteins were incubated with immobilized GST-tagged protein to glutathione sepharose beads (GE Healthcare) at 4°C for binding reactions (for 2h) ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare) and eluted with 0.1M Glycine pH 3.5 ...
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bioRxiv - Biophysics 2020Quote: ... thrombin cleavage of the His6-tag overnight and final ion exchange chromatography with a MonoQ or MonoS column (Äkta purifying system, GE Healthcare, Little Chalfont, United Kingdom). Synaptobrevin 2 49-96 S79C was labeled with Atto488 maleimide ...
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bioRxiv - Microbiology 2020Quote: ... the removal of SUMO protease and SUMO tag from the cleavage reaction was performed with affinity chromatography on a HisTrap HP column (GE Healthcare, Code#:17-5248-01). The SUMO protease and SUMO tag will stay in the HisTrap HP column and the cleaved native protein will be in the flow-through fractions ...
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bioRxiv - Biochemistry 2022Quote: ... The cDNAs of full-length TPR-CHAT were synthesized using the bacterial expression vector pGEX-6P-1 (GE Healthcare, with an N-terminal GST tag). Point mutations were introduced using Fast Site-Directed Mutagenesis Kit in the Sb-gRAMP gene and verified by DNA sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... and Protein G Mag Sepharose (GE healthcare). The DIG-labeled 3’UTR of Emi2 and cyclin B1 RNA probes were synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein A-agarose beads (GE Healthcare, USA) were incubated with the supernatant at 4 °C for 2 h ...
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bioRxiv - Molecular Biology 2019Quote: The beads (G protein Sepharose, GE Healthcare) were washed twice in IP buffer and centrifuged for 2 min at 2’700 x g before blocking with 1 mg/ml BSA in IP buffer for 1 h ...
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bioRxiv - Physiology 2021Quote: ... Protein G Sepharose was from GE Healthcare. Rhodocytin and collagen related peptide (CRP ...
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bioRxiv - Biochemistry 2022Quote: ... and protein A affinity chromatography (GE Healthcare), respectively ...
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bioRxiv - Immunology 2022Quote: ... together with protein A Sepharose (GE Healthcare), respectively ...
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bioRxiv - Immunology 2022Quote: ... Protein A Sepharose (GE Healthcare cat.# 45002982) and Protein G Sepharose (GE Healthcare cat.# 45000118 ...
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bioRxiv - Neuroscience 2020Quote: ... Protein G Sepharose (50 µl, GE Healthcare) was added to the hippocampal lysates and the mixture was rotated at 4°C for 60 min ...
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bioRxiv - Microbiology 2021Quote: ... previously calibrated with protein standards (GE Healthcare), including thyroglobulin (669 kDa) ...
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bioRxiv - Genetics 2019Quote: ... Proteins were detected with ECL (GE Healthcare) using a digital imaging system Image-Lab (Bio-Rad).
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bioRxiv - Molecular Biology 2021Quote: ... precleared with Protein G sepharose (GE Healthcare) for 2 hrs at 4°C and immunoprecipitated overnight with 4 μg of specific antibodies (antibodies listed in Supplementary Table 1) ...
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bioRxiv - Cell Biology 2021Quote: ... or agarose Protein G beads (GE Healthcare) only ...
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bioRxiv - Molecular Biology 2022Quote: ... precleared with Protein G sepharose (GE Healthcare) for 2 hours at 4°C and immunoprecipitated overnight with 4 μg of specific antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... or Protein A HRP (GE Healthcare, NA9120V), all at 1:2500 dilutions (Leng ...
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bioRxiv - Cell Biology 2023Quote: ... G-protein coupled sepharose beads (GE Healthcare) were pre-washed in lysis buffer and blocked overnight in the lysis buffer with 1% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were developed using 1:1 Amersham ECL western blotting reagent (GE Healthcare) to SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... RNAs were then transferred over night to Amersham Hybond N+ membranes (GE Healthcare) by capillarity with 10X SSC transfer buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was transferred to positively charged Nylon transfer membrane (GE Healthcare Life Sciences), immobilized by UV crosslinking ...
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bioRxiv - Immunology 2019Quote: ... and the other gels were transferred to 0.45-μm-nitrocellulose membranes (GE Healthcare) using a mini Tank VEP-2 electroblotting system (Owl Separation Systems ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA was transferred to a nylon membrane (HybondTM-N, GE Healthcare Life Science) by electroblotting in TBE 1X at 8V and 4°C overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... The genomic DNA was transferred to a Hybond N+ membrane (GE Healthcare RPN303B) by vacuum blotting (Boekel Appligene vacuum blotter ...
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bioRxiv - Biochemistry 2020Quote: ... Luminescent signal on PVDF membrane was imaged using ImageQuant LAS-4000 (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... gels were transferred to Amersham Hybond 0.45 µm polyvinyldifluoride (PVDF) membranes (GE Healthcare), blocked with 1% non-fat dry milk in TBS (50 mM Tris-Cl ...
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bioRxiv - Biophysics 2019Quote: ... The gels were then electroblotted on 0.45 um nitrocellulose membrane (GE Life Sciences). The blots were then heated in microwave for 1 min and were blocked with 5% non-fat dry milk solution with 1% Tween-20 in PBS for 1.5h ...
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bioRxiv - Biochemistry 2021Quote: ... After air-drying the membrane was exposed to a Phosphor Screen (GE Healthcare) and the signal visualized using a Typhoon Trio Variable Mode Imager (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... RNA was then electroblotted onto Amersham Hybond-N+ nylon membrane (GE Healthcare, USA) for 90 min at 35V in 0.5x TBE using the TransBlot Mini transfer apparatus (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by overnight capillary transfer to a Hybond-XL membrane (GE Healthcare RPN303S). Telomere probe was labeled using T4 polynucleotide kinase (NEB M0201 ...
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bioRxiv - Microbiology 2021Quote: ... The RNA was transferred overnight to a Hybond N+ nylon membrane (GE Healthcare) by upward capillary transfer ...
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bioRxiv - Neuroscience 2020Quote: ... and then transferred to Amersham Hybond P 0.45 □ μm PVDF membranes (GE Healthcare). The membrane was blocked with 1% non-fat skim milk in Tris-buffered saline containing 0.05% Tween-20 for 1□h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated in Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) and bands were visualized using the ChemiDoc MP imaging system (Bio-Rad).
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were incubated with anti-rabbit horseradish peroxidase-conjugated secondary antibody (GE Healthcare). The ECL Prime (Amersham ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were incubated with anti-rabbit IgG-HRP (GE Healthcare, NA934V, 1:2000) in TBST for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... The membranes were visualized using ECL™ Western Blotting Detection System (GE Healthcare) and ChemiDoc™ Imaging Systems (BioRad Laboratories).