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Citations for GE Life Sciences :
2201 - 2250 of 6458 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Membranes were treated with ECL Western Blotting Detection Reagent (GE Healthcare Life Sciences) and visualized on X-ray film (GE Healthcare Life Sciences ...
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bioRxiv - Genetics 2022Quote: ... Digested DNA was transferred by capillarity to a Hybond-N membrane (GE Healthcare) and hybridized with 32P-labeled probe which is specific to CaLCuV DNAA ...
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bioRxiv - Plant Biology 2022Quote: ... The RNA was transferred onto a neutral nylon membrane (Hybond-NX, GE Healthcare) and crosslinked using 1-ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Molecular Biology 2022Quote: ... and then transferred to Amersham Hybond P 0.45 µm PVDF membranes (GE Healthcare). The membrane was blocked with 5% non-fat skim milk in Tris-buffered saline containing 0.05% Tween-20 for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... the membranes were treated with ECL Select Western Blotting Detection Reagent (GE Healthcare), and the chemiluminescent signal on the membranes was detected using an ImageQuant LAS 500 imager system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... the agarose gel was transferred to a Hybond-N+ nylon membrane (GE Healthcare). An 819-bp fragment containing the full length sZF cDNA was labeled by random priming with [α-32P] dCTP and used as a probe.
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bioRxiv - Molecular Biology 2022Quote: ... 300 V and transferred onto a Hybond-N+ membrane (GE Healthcare Life Sciences) at 4 °C for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... and transferred onto AmershamTM ProtranTM Premium 0.45 μm nitrocellulose membranes (GE Healthcare, GE10600048) for 16 h at 30V ...
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bioRxiv - Plant Biology 2022Quote: ... followed by semi-dry transferring onto Hybond-N+ hybridization membranes (GE Healthcare, RPN303B). The membranes were UV cross-linked and hybridized with miRNA probes labeled with [γ-32P] ATP (PerkinElmer) ...
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bioRxiv - Cell Biology 2022Quote: Samples were spotted onto a 0.22 µm pore size nitrocellulose membrane (GE Healthcare) and allowed to dry ...
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bioRxiv - Immunology 2023Quote: ... The nitrocellulose membrane was imaged with the ImageQuant™ LAS 4000 (GE Healthcare). Quantification was done using the open image analysis software ImageJ ...
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bioRxiv - Molecular Biology 2023Quote: ... and transferred onto nitrocellulose membranes (Amersham Hybond, 10600021, GE Healthcare, Chicago, IL, USA) using Trans-Blot SD Semi-dry Transfer Cell system (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... Nitrocellulose membranes (8 μm) were from Cytiva (formerly GE Healthcare Life Sciences, UK) and silicon metal powder (3 μm ...
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bioRxiv - Molecular Biology 2023Quote: ... with suitable percentage then transferred on nitrocellulose membranes (GE Healthcare or Bio-Rad). Membranes were blocked with 5% non-fat milk in TBS-T (10 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... onto 0.22 um Amersham™ Protran™ nitrocellulose membranes (GE Healthcare Life Science) at 30 V and 4 °C for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... and transferred to nitrocellulose membranes (0.45 μm pore size; GE Healthcare, Chicago, IL). To evaluate the reactivity of each mAb ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then scanned using an Amersham Typhoon 5 imager (GE Healthcare) with a 685 nm laser (IRshort720BP20 filter ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were visualized by using CDP- Star Chemiflourescent detection system (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... and transferred onto nitrocellulose membranes (Amersham Protran 0.2μm NC; GE Healthcare Life Sciences). In some cases ...
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bioRxiv - Cell Biology 2023Quote: ... Reaction mixtures were spotted onto filter sheets (Amersham Protran nitrocellulose membrane, GE Healthcare) and washed 3 x with PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The material was then transferred onto Amersham Protran Nitrocellulose Blotting Membrane (GE Healthcare) using a transfer buffer containing 1 x NuPAGE (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA was transferred overnight to a Hybond-N+ nylon membrane (GE Healthcare) by upward capillary transfer with 20x SSC buffer ...
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bioRxiv - Biophysics 2023Quote: ... transferred to a nitrocellulose membrane (AmershamProtran 0.45 µM NC; GE Healthcare Life Sciences), and blocked in 5 % BSA at room temperature for 1h ...
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bioRxiv - Cancer Biology 2024Quote: ... After equilibration and washing of a nitrocellulose membrane 0.2 µm (GE Healthcare, 10600001) in SSC 10x buffer (NaCl 3M ...
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bioRxiv - Plant Biology 2023Quote: ... the RNA was blotted onto the HybondTM-N+ nylon transfer membrane (GE Healthcare) by soaking in 20× SSC (3.0 M NaCl and 0.3 M sodium citrate ...
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bioRxiv - Genetics 2024Quote: ... membranes were incubated with AmershamTM ECLTM Prime Western Blotting Detection Reagent (GE Healthcare) and imaged with an VWR® CHEMI only system (VWR International) ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... Electroblotting of the RNA onto a Hybond-N+ membrane (GE Healthcare Life Sciences) was performed at 50 V ...
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bioRxiv - Molecular Biology 2023Quote: ... Small RNAs were electro-transferred onto a nylon Hybond-NX membrane (GE Healthcare) in 0.5X Tris-Borate-EDTA buffer ...
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bioRxiv - Microbiology 2021Quote: ... Protein-DNA immunocomplexes were retrieved by incubation with 60 µl of equilibrated protein G-conjugated agarose beads (GE Healthcare), for 2 h at 4⁰C ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated with a rabbit polyclonal anti-HDA15 antibody and protein G Mag Sepharose beads (GE Healthcare) overnight at 4°C ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... The GST-tagged proteins were expressed and purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) following previously published protocols (42) ...
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bioRxiv - Cancer Biology 2022Quote: ... All protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare). Further details can be found in our previous publication [14].
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were clarified followed by protein A affinity chromatography with MabSelect SuRe LX protein A resin (GE Healthcare) using the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... 6His–GST–CRK2cyto (WT, K353E and D450N) were expressed in Escherichia coli Lemo21 and purified by Glutathion Sepharose 4B (GE Healthcare, Chicago, USA). 6His–MBP–PLDα1 and 6His–MBP–CALS1_N were expressed in E ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM UltraGlutamine (GE Healthcare, UK), BME vitamins (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2 mM 2-mercaptoethanol for 12 h and purified by anion exchange chromatography (HiTrap Q, GE Healthcare) with a linear gradient of 0 to 500 mM NaCl over 40 min and size-exclusion chromatography (HiLoad 16/600 Superdex 200 ...
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bioRxiv - Microbiology 2020Quote: ... proteins were immobilized to a protein A sensorchip a level of ~500 response units (RUs) using Biacore 8000 (GE Healthcare) and a running buffer of composed of 20mM PB7.4 with 300 mM NaCl ...
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bioRxiv - Biophysics 2019Quote: The proteins and protein complexes were analyzed by analytical gel filtration using a Superdex 200 5/150 column (GE Healthcare) and the 1260 Infinity Bio-inert high-performance liquid chromatography system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences). The eluted fractions were collected for SDS-PAGE analysis.
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was further purified by fast protein liquid chromatography (FPLC) using a Source-S cation-exchange column (GE Healthcare) (low salt buffer with 50 mM potassium phosphate at pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were further purified by gel filtration on a fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principle peaks were collected ...
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bioRxiv - Zoology 2021Quote: ... Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare) as per manufacturer’s instructions (Figure A1 ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was added to 50 µl G-Protein Sepharose beads (Protein G-Sepharose 4 Fast Flow, GE Healthcare) (pre-washed with the lysis buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein G-coupled sepharose (#17–0618–01) and Protein A-coupled sepharose (#17–5138–01) beads were from GE Healthcare and the protease inhibitor cocktail was from Sigma.
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected with protein-specific primary antibodies and HRP-coupled secondary antibodies by enhanced chemiluminescence (GE Healthcare) and imaged using X-ray films or with Fusion Capture Advance FX7 16.15 (Peqlab).
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred onto a Hybond P 0.45 polyvinylidene difluoride (PVDF) membrane (GE life sciences) using wet blotting ...
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bioRxiv - Cell Biology 2020Quote: ... The gel was subsequently electroblotted onto a nylon membrane (Amersham Hybond N+, GE Healthcare) as described 55 ...