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Citations for GE Life Sciences :
1901 - 1950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Anion exchange chromatography (HiTrap Q HP, GE Healthcare) was performed with a mobile phase of 25 mM Tris pH 7.6 and a linear gradient of 9 column volumes of elution buffer to 1M NaCl on an Äkta Pure 25M (Cytiva) ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% heat-inactivated FCS (HyClone, GE Healthcare, SV30160.03), 100 U/mL penicillin and 100 µg/mL streptomycin (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... 40μg of each protein extract (total or nuclear protein extract) were fractionated by SDS–PAGE and transferred to PVDF membranes (GE Healthcare Life Sciences, Buckinghamshire, UK). These were blocked in 5% nonfat dry milk in TBS-T (20mM Tris-HCl pH=7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected with enhanced chemiluminescence (ECL) detection reagents (RPN2236 or RPN2235, Amersham Biosciences, GE Healthcare,) and ChemiDoc™ XRS+ System (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... The protein bands were detected by incubating the membrane with primary antibodies (see below) overnight at +4°C and horse radish peroxidase-conjugated secondary antibodies (1:5000, NA934 or NA931, GE Healthcare) for 1 h at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Blots were developed using the ECL Detection Reagent (GE Healthcare Life Sciences), images were acquired on a ImageQuant LAS 4000 (GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2024Quote: ... images were acquired on a ImageQuant LAS 4000 (GE Healthcare Life Sciences) and analyzed using Image J 1.52p (National Institute of Health ...
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bioRxiv - Molecular Biology 2024Quote: ... Membrane signals were developed and quantified using the ImageQuant ™ TL 8.1 software (GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were purified on the Äkta Start system (GE Healthcare) using metal affinity chromatography resins (Talon column) ...
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bioRxiv - Molecular Biology 2024Quote: ... methanol-based wet transfer and chemiluminescence detection (Amersham ECL detection reagent, GE Healthcare). Primary antibodies for PAR1 (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... Final separation was achieved on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in gel filtration buffer (20 mM HEPES-KOH ...
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bioRxiv - Microbiology 2024Quote: ... 20-40 µg was added to a Superose 6 increase 10/300 column (GE Healthcare) and MALS spectra was recoded using inline DAWN-HELEOS light scattering and optilab T-rEX refractive index detectors (Wyatt) ...
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bioRxiv - Microbiology 2024Quote: ... or mixed protein and DNA were analysed by analytical gel filtration (a-SEC) using a Superdex 200 Increase 10/300 GL column (GE Healthcare) running in DNA binding buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... 300 mM imidazole) and further purified using a 1 ml Source 15Q column (GE Healthcare), running in buffer A (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... The OD600 of culture was measured by a spectrophotometer (GeneQuant1300; GE HealthCare, IL, United States) with 1-h intervals ...
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bioRxiv - Microbiology 2024Quote: ... Sectional images were captured and deconvoluted using a DeltaVision Elite system (GE Healthcare, Chicago, IL, USA) with a 60× oil-immersion objective on an Olympus IX71 microscope.
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bioRxiv - Microbiology 2024Quote: ... Purification was performed by using an MBPTrapHP column (GE Healthcare) and His-Select Ni Affinity Gel (Sigma).
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bioRxiv - Microbiology 2024Quote: ... The secondary antibodies used were horseradish peroxidase-conjugated anti-mouse (NA931; GE Healthcare) and anti-goat (ab6741 ...
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bioRxiv - Microbiology 2024Quote: ... IgG Sepharose 6 Fast Flow beads (20 µl, GE Healthcare) were equilibrated by one wash step using 1 ml of ice-cold TST buffer (50 mM Tris pH 7 ...
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bioRxiv - Microbiology 2024Quote: 30 µl Protein G Sepharose 4 Fast Flow beads (GE Healthcare) were washed with 1 ml of PBS and twice with 1 ml of PBS/1% BSA ...
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bioRxiv - Biochemistry 2024Quote: ... 50 μL of GTL25D1/COLGALT1 concentrated at 4 mg/mL were injected into a Superdex 200 PC 3.2/300 Increase column (GE Healthcare), pre-equilibrated with 25 mM HEPES/NaOH ...
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bioRxiv - Biochemistry 2024Quote: ... After incubation with ECL Western blotting reagent (GE Healthcare), images were acquired with the ChemiDoc MP System (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Elution fractions containing the most protein were combined and loaded directly onto a size-exclusion column (GE Healthcare) pre-equilibrated with lysis buffer without prior concentration or dialysis of the protein ...
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bioRxiv - Immunology 2024Quote: PBMCs were isolated from peripheral blood at GHESKIO (Port-au-Prince, Haiti) using the Ficoll-Paque (GE Healthcare) density centrifugation ...
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bioRxiv - Immunology 2024Quote: ... An affinity matrix was prepared by coupling 50 mg of His-tagged GST-RAP to 20 ml of NHS-activated Sepharose 4 Fast Flow (GE Healthcare), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were separated from buffy coats using Ficoll-Paque Premium (GE Healthcare) density gradient separation ...
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bioRxiv - Immunology 2024Quote: ... human peripheral blood mononuclear cells were recovered by gradient centrifugation on Ficoll-Paque Plus (GE Healthcare) and monocytes were further purified using CD14 microbead positive selection and MACS separation columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll (17-1440-03; GE Healthcare) density gradient centrifugation from blood of healthy volunteers.
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bioRxiv - Immunology 2024Quote: ... ShH10 particles were bound to a 1-mL HiTrap AVB Sepharose column (GE Healthcare, USA) and eluted with 50 mM glycine (pH 2.7 ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from buffy coats by Ficoll-Paque (GE Healthcare) density-gradient and washed twice with RPMI-1640 with L-glutamine (RPMI ...
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bioRxiv - Immunology 2024Quote: ... 0–1.0 M NaCl gradient) FPLC columns (GE Healthcare).
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bioRxiv - Immunology 2024Quote: ... or NRAS–HLA-A1 ligand was immobilized on a streptavidin-coated BIAcore SA chip (GE Healthcare) at approximately 1000 resonance units (RU) ...
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bioRxiv - Immunology 2024Quote: ... and biotinylated monomers purified by ion exchange chromatography using an AKTA FPLC system (GE Healthcare, Chicago, IL, USA). The final product was verified by denaturing SDS-PAGE ...
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bioRxiv - Microbiology 2024Quote: ... horseradish peroxidase-conjugated anti-mouse (NA931; GE Healthcare, Chicago, IL), anti-rabbit (NA934 ...
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bioRxiv - Microbiology 2024Quote: ... anti-rabbit (NA934; GE Healthcare), and 6-nm gold-conjugated anti-rabbit (711-195-152 ...
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bioRxiv - Cancer Biology 2024Quote: ... A549-luc cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum (GE Healthcare Life Sciences, HyClone Laboratories) and 1% penicillin-streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... cells were imaged using a UPLSAPO 100x / 1.4NA oil objective on a DeltaVision deconvolution microscope (GE Healthcare, UK) equipped with an IX71 inverted microscope (Olympus ...
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bioRxiv - Microbiology 2024Quote: ... in an ImageQuant LAS 4000 mini biomolecular imager (GE Healthcare). The signal intensity corresponding to protein bands was quantitatively analyzed with ImageJ software (81).
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bioRxiv - Immunology 2024Quote: Ficoll-Paque PLUS (GE Healthcare, Ref. 17144003).
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bioRxiv - Microbiology 2024Quote: Single Cononympha cells were collected using a Leica AM6000 micromanipulation system and subjected to whole genome amplification (WGA) using the illustra GenomiPhi V2 kit (GE Healthcare) as described previously [30] ...
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bioRxiv - Immunology 2024Quote: ... IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare, Life Sciences) dependent on culture volume ...
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bioRxiv - Immunology 2024Quote: ... Fabs were initially purified using a HisTrap Excel 5 mL column (GE Healthcare, Life Sciences), and eluted in 5 mL 0.5 M NaCl / 10 mM PBS/ 250 mM Imidazole pH7.4 ...
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bioRxiv - Immunology 2024Quote: ... Eluted samples with high concentration or in excess of 10-20 mL volume were buffer exchanged using two HiPrep 26/10 Desalting columns (GE Healthcare, Life Sciences) in series ...
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bioRxiv - Immunology 2024Quote: ... Eluate was further purified by SEC in TBS using a HiLoad 16/600 200pg column (GE Healthcare, Life Sciences).
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bioRxiv - Immunology 2024Quote: ... Whole blood was layered on Ficoll-Paque Plus (GE Healthcare) in 50mL SepMate tubes (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 7.5% iron-enriched BCS (GE Healthcare HyClone) for 24 h and then used for a time-course of release by adding biotin (#4698 ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% iron-enriched bovine calf serum (BCS; GE Healthcare HyClone), 120 µg/ml penicillin ...
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bioRxiv - Cell Biology 2024Quote: ... and transferred to Optitran BA-S83 nitrocellulose membrane (GE healthcare life science). After blocking in PBS containing 5% (wt/vol ...
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bioRxiv - Cell Biology 2024Quote: Antigen-antibody and biotin-streptavidin complexes were detected by using the Amersham ECL Western Blotting Detection Reagent (Cytiva, RPN2235) in combination with the ImageQuant LAS4000 system (GE Healthcare). Images were further processed and quantified with AlphaView Software (ProteinSample ...
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bioRxiv - Cancer Biology 2024Quote: ... transferred to nitrocellulose membranes (GE healthcare), immunoblotted with specific primary and secondary antibodies and detected by chemiluminescence with the ECL detection reagents from Thermo Fisher or Millipore ...