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Citations for GE Life Sciences :
2051 - 2100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Clear cell lysates (clarified by centrifugation) were purified with Ni-HiTrap FF and MBPtrap HP columns (GE Healthcare). Then tag-cleaved proteins was purified with ion exchange chromatography (HiTrap SP FF column (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... NTPs were purchased from GE Healthcare (Mississauga, ON, Canada). [α-32P]GTP was purchased from PerkinElmer (Revvity ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a GSTrap column (GE Healthcare) equilibrated in lysis buffer ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... the protein was loaded onto a Source 30Q column (GE Healthcare) equilibrated in low salt buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Target proteins in the HiTrap SP FF column were eluted by a linear gradient of NaCl and further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare). The purified proteins were of high purity (>95 % ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... Fractions containing target protein were pooled and incubated over night at 4°C with PreScission protease (GE Healthcare) at a ratio of 1:100 (w/w ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... Fractions containing the target protein were pooled and loaded onto a Superdex S75 column (GE Healthcare) equilibrated in buffer containing 150 mM KCl ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... The flow-through containing the cleaved eIF2β was pooled and applied to a Superdex S200 column (GE Healthcare) equilibrated in a buffer containing 150 mM KCl ...
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bioRxiv - Biochemistry 2024Quote: ... exposed with Super RX Medical X-Ray Film (FUJI) and autoradiograph was developed using a Typhoon scanner phospho imager (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... 2 mM TCEP) and incubated for 1 hour at 4°C before loading on Superose 6 5/150 column (GE Healthcare) equilibrated with the same buffer on an AKTA-micro system (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... or a Superose 6 Increase Column (GE Life Sciences). Purified protein was snap frozen and stored at –80°C until required ...
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bioRxiv - Biochemistry 2024Quote: ... coli and purified using glutathione Sepharose 4B beads (GE Healthcare). Purified CKM (3 μM ...
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bioRxiv - Biochemistry 2024Quote: ... Free biotin was removed using size-exclusion chromatography by loading the labelled protein on a Superose 6 gel filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on a 5 ml HiTrap Q HP column (GE Healthcare, Pittsburgh, PA), and the flow-through was collected and dialyzed against 20 mM HEPES pH 6.8 ...
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bioRxiv - Biochemistry 2024Quote: ... was added to the supernatant and purification was carried by Ni2+-affinity chromatography using NTA-agarose column (Chelating Sepharose TM, Fast Flow, GE Healthcare). The sample was loaded and washed with 500 mL lysis buffer (25 mM Tris pH 7.9 ...
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bioRxiv - Biochemistry 2024Quote: ... by using a Ni2+-resin column (HisTrap-HP 1 ml, GE Healthcare) as described previously (48).
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bioRxiv - Biochemistry 2024Quote: ... or a nano-grid control slide (GE Healthcare) were used to measure axial and lateral chromatic misregistration ...
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bioRxiv - Biochemistry 2024Quote: ... Excess HaloTag ligand was then removed by purification on desalting NAP-5 or PD-10 columns (GE Healthcare). To generate the HRP-his6Halo-hPKR-N conjugate ...
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bioRxiv - Biochemistry 2024Quote: ... pH 7.4 at room temperature) concentrated and loaded onto a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) equilibrated in MBP-loading buffer ...
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bioRxiv - Neuroscience 2024Quote: ... an NAP-10 desalting column (GE Healthcare) was washed with deionized water (20 mL) ...
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bioRxiv - Biochemistry 2024Quote: ... the clarified lysate was incubated with 25 ul glutathione sepharose 4B beads (GE Healthcare) for 30 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Peptide fraction was performed on AKTA pure 25 with Superdex 30 Increase 3.2/300 (GE Healthcare) at a flow rate of 30 μl min-1 of the elution buffer with a 100 μl fraction volume ...
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bioRxiv - Biochemistry 2024Quote: ... 3 mM NaN3) on a Superdex 200 Increase 5/150 column (GE Healthcare) equilibrated by the same buffer and run at a 0.45 ml/min flow rate ...
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bioRxiv - Biochemistry 2024Quote: ... IMAC was performed with Ni-NTA resin (GE Healthcare, Chicago, IL, USA) in disposable PD-10 columns ...
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bioRxiv - Biochemistry 2024Quote: ... GST (1:5,000, GE Healthcare 27457701), HA (1:2,000 ...
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bioRxiv - Biochemistry 2024Quote: ... and high-resolution image acquisition using ImageQuant LAS 4000 (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2024Quote: An AKTA FPLC system (GE Healthcare) was employed to conduct analytical size exclusion chromatography (SEC) ...
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bioRxiv - Biochemistry 2024Quote: ... and gel filtration chromatography (HiLoad 26/60 Superdex 75 pg, GE Healthcare). Finally ...
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bioRxiv - Biochemistry 2024Quote: In vitro pull-down binding assays showing the interaction between MBP-PR18 and Kapβ2 were performed using GST-Kapβ2 immobilized on GSH sepharose beads (GE Healthcare). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... and subsequently subjected to size exclusion chromatography using Superdex 200 prep grade column (GE Healthcare, UK) equilibrated with PBS ...
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bioRxiv - Biochemistry 2024Quote: ... MSP1E3D1 protein was purified using Chelating Sepharose FF (GE Healthcare, UK) and dialyzed against MSP standard buffer (20 mM TRIS-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... The fluorescently labeled RNAs were visualized using Amersham Typhoon (GE healthcare).
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bioRxiv - Biochemistry 2024Quote: ... The dialyzed sample was purified using size-exclusion chromatography (Superdex 200 10/300, GE Healthcare Life Sciences, USA). Peak fractions ...
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bioRxiv - Biochemistry 2024Quote: ... The cleaved sample was passed through an equilibrated Nickel-charged IMAC using a 5 mL HisTrap HP (GE Healthcare Life Sciences), and the flowthrough was collected ...
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bioRxiv - Biochemistry 2024Quote: ... The filtered soluble cell solution was passed through an equilibrated Nickel-charged IMAC using a 5 mL HisTrap HP (GE Healthcare Life Sciences) in Lysis Buffer ...
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bioRxiv - Biochemistry 2024Quote: ... and the HiLoad 26/600 Superdex 200 column (GE Healthcare Life Sciences) was equilibrated in size-exclusion buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Antibodies were separated from unincorporated fluorophores on desalting columns (NAP-5 or PD-10, GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... Further purity was achieved by gel filtration (HiLoad Superdex 200 26/60, GE Healthcare) (Buffer ...
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bioRxiv - Biochemistry 2024Quote: ITC was performed at 25 with a stirring rate of 750 rpm using a MicroCal iTC 200 calorimeter (GE Healthcare). Stock solutions of α-actin and AgADF were dialyzed extensively against 5 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... the protein elution was further purified by Superdex 200 Increase 10/300 GL (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... The concentrated and filtered sample was then finally passed through a HiLoad 10/300 Superdex 75 column (GE Healthcare, Chicago, IL, USA) equilibrated with SEC buffer [20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2024Quote: ... Final purification was performed with size exclusion chromatography using a Superdex 200 pg 16/600 gel filtration column (120 ml bed volume, GE Healthcare). At every purification step ...
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bioRxiv - Biochemistry 2024Quote: ... Native purification of 6 x His-tagged chDHRSX was performed using the AKTA purifier 900 series (GE Healthcare) by using a Ni2+-resin column (HisTrap-HP 1 ml ...
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bioRxiv - Biochemistry 2024Quote: ... pGEX-2tk (GE Healthcare) or pET30-HT7 ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were exposed to phosphor screens overnight and imaged using Typhoon FLA 7000 (GE healthcare Life Sciences).
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bioRxiv - Biochemistry 2024Quote: Gels were exposed to phosphor screens overnight and imaged using Typhoon FLA 7000 (GE healthcare Life Sciences). Bands corresponding to leftover pri-miRNA substrate ...
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bioRxiv - Biochemistry 2024Quote: ... using a Superdex200 Increase 10/300 GL column (GE Healthcare) equilibrated in 20 mM MES pH 6 ...
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bioRxiv - Microbiology 2024Quote: ... SP3 beads were obtained from GE Healthcare (Chicago, USA). 1 µg trypsin (Promega ...
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bioRxiv - Immunology 2024Quote: ... Response units were recorded at room temperature with BIAcore 8K Evaluation Software (v4.0.8.20368; GE Healthcare). The data were analyzed and fitted to a 1:1 binding model using the same software.
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bioRxiv - Genetics 2024Quote: ... M346V and M346T LBDs (residues 182-447) were cloned into a pGEX2T (GE Healthcare) vector containing an amino terminal GST purification tag followed by a TEV protease cleavage site ...