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Citations for GE Life Sciences :
1751 - 1800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... coli BL21(DE3) and purified by Glutathione Sepharose 4B (GE Healthcare) as described previously (Nagamori et al. ...
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bioRxiv - Biochemistry 2024Quote: ... affinity columns of GST-fused Asct2(NT)-antigen and GST-fused Asct2(CT)-antigen were produced by conjugating the antigens with HiTrap NHS-activated HP (GE Healthcare). To purify anti-Asct2(NT ...
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bioRxiv - Biochemistry 2024Quote: ... Anti-Smct1 Ab was purified using HiTrap Protein G HP (GE Healthcare) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The selected fractions were pooled and loaded onto a Superdex75 size exclusion chromatography (GE Healthcare) equilibrated in the same buffer ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µg of protein was immobilized on a nitrocellulose membrane (Amersham) by filtration using a slot blot apparatus (GE Healthcare). The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam ...
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bioRxiv - Cell Biology 2024Quote: Cells were grown in 1:1 DMEM high glucose: Ham’s F-12 (Hyclone, GE Healthcare) supplemented with 5% fetal bovine serum (Hyclone) ...
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bioRxiv - Bioengineering 2024Quote: ... Data were fitted using 1:1 Langmuir binding model within the Biacore 8K analysis software (GE Healthcare #29310604).
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bioRxiv - Bioengineering 2024Quote: ... 0.005% v/v Surfactant P20 (GE Healthcare). Scaffolds and RSVF trimer or IgG proteins were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
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bioRxiv - Bioengineering 2024Quote: ... and 300 mM Imidazole (pH 7.5) and subsequent size exclusion chromatography on a HiLoad 16/600 Superdex 75 column (GE Healthcare) in PBS buffer.
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bioRxiv - Bioengineering 2024Quote: ... Final purification by size exclusion chromatography in PBS (pH 7.4) on a Superdex 200 Increase 10/300 GL column (GE Healthcare).
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bioRxiv - Bioengineering 2024Quote: SPR measurements were performed on a Biacore 8K (GE Healthcare) in 10 mM HEPES pH 7.4 ...
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bioRxiv - Bioengineering 2024Quote: ... The eluted protein was further purified on a StrepTrap HP affinity column (GE Healthcare) eluted using 10 mM Tris ...
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bioRxiv - Bioengineering 2024Quote: ... Scaffolds and RSVF trimer or IgG proteins were immobilized on a CM5 chip (GE Healthcare # 29104988) by amine coupling ...
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bioRxiv - Cancer Biology 2024Quote: ... and the Superdex 200 10/300 GL (GE Healthcare). The column was equilibrated with 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... using a CCD camera (LAS 4000, FujiFilm or AI600, GE Healthcare).
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bioRxiv - Biophysics 2024Quote: ... for 45 min at 4 °C and loaded on an empty PD-10 column (GE Healthcare). The column was washed with 15 column volumes of wash buffer containing 20 mM HEPES ...
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bioRxiv - Biophysics 2024Quote: ... The column was washed with 40 column volumes of the same buffer (without MTSL) and further eluted with 300 mM imidazole and immediately deslated using a PD-10 desalting column (GE Healthcare) into the same buffer ...
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bioRxiv - Biophysics 2024Quote: ... After size-exclusion chromatography with a Superdex 200 Increase10/300 GL column (GE Healthcare), the protein fractions were collected and concentrated to ∼40 µM protein ...
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bioRxiv - Biophysics 2024Quote: ... Dialysed protein was further purified by ion-exchange chromatography using a HiTrap-FF Q column (GE Healthcare), resulting in the elution of N-myc proteins in the flow-through and at low salt concentrations ...
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bioRxiv - Biophysics 2024Quote: ... N-myc proteins were polished using size-exclusion chromatography (SEC) using a Superdex 16/600 S75 column (GE Healthcare). GB1-tagged N-myc proteins were purified in a similar manner with a few alterations ...
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bioRxiv - Biophysics 2024Quote: ... further contaminants were separated by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare). Protein-containing fractions were dialyzed against Storage Buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... 0.0025% P-20 surfactant (GE healthcare), 2 mM ATP (unless stated otherwise) ...
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bioRxiv - Biophysics 2024Quote: ... the supernatant was purified by IMAC (5 mL HiTrap Chelating HP, GE Healthcare) using an FPLC system (ÄKTAprime ...
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bioRxiv - Biophysics 2024Quote: ... Collected nanobody fractions were then fractionated by size exclusion chromatography (SEC) using a Superdex 75 Increase 10/300 GL column (GE Healthcare) in HBS buffer.
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bioRxiv - Biophysics 2024Quote: ... using an FPLC system (ÄKTAprime, GE Healthcare). Proteins were eluted by a linear gradient against HBS buffer containing 500 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... Supernatant was mixed with GFP nanobody-conjugated Sepharose 4 Fast Flow resin (GE Healthcare). Resin was washed with buffer containing 0.06% digitonin ...
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated using a 100 kDa filter to approximately 1 mL and further purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare) equilibrated with buffer containing 0.06% digitonin ...
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bioRxiv - Biophysics 2024Quote: The adjusted pool was loaded onto a 1 ml Phenyl-HP hydrophobic interaction column (GE Healthcare) pre-equilibrated with PepA Buffer D then washed with the same buffer ...
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bioRxiv - Biophysics 2024Quote: ... The crude PepA solution was loaded onto a HiTrap Heparin-HP 1 ml column (GE Healthcare), then washed with PepA Buffer A [PepA Buffer C without Glycerol] ...
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bioRxiv - Biophysics 2024Quote: ... using a 1 ml HisTrap-HP column (GE Healthcare), then by a Heparin-affinity purification using a 1 ml HiTrap Heparin-HP column (GE Healthcare) ...
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bioRxiv - Biophysics 2024Quote: ... then by a Heparin-affinity purification using a 1 ml HiTrap Heparin-HP column (GE Healthcare). Other FPLC steps from Subramanya et al ...
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bioRxiv - Biophysics 2024Quote: ... Pooled elution fractions were subjected to a 2nd FPLC stage using a 1 ml HiTrap Heparin-HP 1 ml column (GE Healthcare). The bound MBP-XerC was washed using heparin loading buffer [50 mM Tris pH 8 ...
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bioRxiv - Biophysics 2024Quote: ... followed by anion exchange chromatography using a MonoQ 5/50GL (GE Healthcare) column ...
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bioRxiv - Biophysics 2024Quote: ... After papaïn inactivation with FBS (100 U/mL, GE Healthcare), structures were mechanically dissociated with a pipette (plastic tip 1 mm diameter ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein bands were detected using an Amersham Imager 600 UV (GE Healthcare, Eindhoven, Netherlands) and visualized using ImageJ-Fiji software (version 1.53t ...
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bioRxiv - Cancer Biology 2024Quote: Time-lapse microscopy images were acquired using an IN Cell Analyzer 2500HS (GE Healthcare Life Science) equipped with a CFI S Plan Fluor ELWD dry objective lens 20× (NA ...
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bioRxiv - Cancer Biology 2024Quote: ... After pre-clearance with G-Sepharose beads (GE Healthcare, Chicago, IL, USA), 2 µg of BRCA1 or IgG antibody were added and incubated at 4°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... and detected via chemiluminescence using ECL detection reagent (GE Healthcare, RPN2209).
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bioRxiv - Cancer Biology 2024Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated using the Ficoll-Paque gradient (GE Healthcare) from the blood of healthy donors after informed consent ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was purified in two steps using an ÄKTA FPLC system (GE Healthcare Biosciences): a ...
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bioRxiv - Microbiology 2024Quote: ... b) size-exclusion chromatography equipped with a Superdex 200 16/600 column (GE Healthcare Life Sciences) pre-equilibrated with SEC buffer (50 mM sodium phosphate ...
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bioRxiv - Microbiology 2024Quote: ... 5 mL column (GE Healthcare Life Sciences), which was first washed with 100 mL Wash buffer (50 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... with Ni-NTA research grade sensor chip (GE Healthcare, USA). The temperature was maintained at 25°C for analysis and sample compartments for all the binding and kinetic experiments ...
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bioRxiv - Microbiology 2024Quote: SPR studies for studying binding kinetics were carried out using BIACORE T200 (GE Healthcare, USA) with Ni-NTA research grade sensor chip (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... or detected with ImageQuant LAS 4000 mini imager (GE Healthcare), followed by its reaction with detection reagents (ECL Western Blotting Detection Reagents ...
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bioRxiv - Cell Biology 2024Quote: ... followed by its reaction with detection reagents (ECL Western Blotting Detection Reagents, GE Healthcare or SuperSignalTM West Dura ...
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bioRxiv - Molecular Biology 2024Quote: ... Digested samples were passed through pre-washed (4x with 700 μl polysome buffer for 1 min at 450 x g) Sephacryl S-400 HR spin columns (GE healthcare, currently Cytiva), and centrifuged for 2 min at 450 x g at 4°C ...
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bioRxiv - Cell Biology 2024Quote: Shh size distribution in the presence or absence of soluble carriers was confirmed by SEC analysis with a Superdex200 10/300 GL column (GE Healthcare, Chalfornt St. Giles, UK) equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
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bioRxiv - Cell Biology 2024Quote: ... Reduction of disulfide bonds and alkylation of cysteine residues was performed by incubating gel fragments in 10 mM 1,4-Dithio-D-threitol (GE Healthcare; cat. #17-1318-02) at 50°C for 1 hour followed by a light-protected incubation with 55 mM iodoacetamide (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... at 50°C for 1 hour followed by a light-protected incubation with 55 mM iodoacetamide (GE Healthcare; cat. #RPN6302V) at room temperature for 45 min ...