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Citations for GE Life Sciences :
1701 - 1750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... for 18 h at 18°C and N-terminal His6-tag fusion proteins purified by step-wise affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM tris-HCl (pH 7.4) ...
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bioRxiv - Biophysics 2024Quote: ... The imidazole was removed by desalting column (PD MiniTrap G-25, GE Healthcare). Final protein concentration was determined by A280 using an extinction coefficient of 75000 M-1cm-1 (ACKR3 ...
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bioRxiv - Cancer Biology 2024Quote: ... and Amersham’s enhanced chemiluminescence Western blotting detection reagent (GE Life Sciences). A horseradish peroxidase-conjugated β-actin antibody was used to detect this protein loading control (clone 8H10D10 ...
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bioRxiv - Biochemistry 2024Quote: ... 40 µL of the mixture was injected into a Superdex 75 10/300 GL gel filtration column (GE Healthcare) pre-equilibrated with the Rab buffer with a flow rate of 0.5 mL/min at room temperature and absorption at 280 nm was recorded.
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bioRxiv - Bioengineering 2024Quote: ... The column was attached to an ÄKTA Pure FPLC (GE Healthcare Biosciences, Sweden) equipped with a P960 sample pump and analyzed with UNICORN 5.0 software (Cytiva Life Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... The cleared lysates were loaded onto Ni-Sepharose (GE Healthcare) and eluted with denaturing buffer or non-denaturing buffer supplemented with 500 mM imidazole ...
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bioRxiv - Neuroscience 2024Quote: ... and ImageQuant 800 (GE Healthcare). For western blot on synaptosomes ...
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bioRxiv - Molecular Biology 2024Quote: ... The signal was amplified with ECL substrate (GE Healthcare, RPN2106) and detected in Image Quant LAS4000 using a CCD camera.
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bioRxiv - Cell Biology 2024Quote: ... GST-Ubl Parkin was purified using Glutathione Sepharose™ 4B (GE Healthcare #17-0756-01), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The labeled antibodies were then purified using a NAP-5 column (17085302, GE Healthcare) and determined by a UV-Vis spectrophotometer.
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bioRxiv - Bioengineering 2024Quote: ... from which key cardiac cycle events were identified using EchoPac software (version 214, GE Healthcare) to temporally align data for postprocessing ...
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bioRxiv - Plant Biology 2024Quote: ... and chemiluminescent signals were detected with ImageQuant LAS 4000 (GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... samples were imaged with an InCell Analyzer 2200 HTM (GE Healthcare). Quantification of cellular cholesterol was done using the Cholesterol/Cholesterol Ester-Glo™ kit (Promega ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and detected in an Amersham™ Imager 600 (GE Healthcare). A full list of the antibodies used in this study is available in Table S1 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Image acquisition was done using an IN Cell Analyzer 2200 (GE Healthcare) high-throughput microscope ...
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bioRxiv - Neuroscience 2024Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 or Amicon 15 centrifugal concentrators (10 kDa, 5 kDa, or 3 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Neuroscience 2024Quote: ... 10% fetal bovine serum (FBS, GE Healthcare), 1X MITO+ Serum Extender (Corning) ...
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bioRxiv - Neuroscience 2024Quote: ... were obtained using a DeltaVision Restoration Microscopy System (Applied Precision/GE Life Sciences) with an inverted Olympus IX70 microscope with a 20 × oil objective ...
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bioRxiv - Neuroscience 2024Quote: ... were obtained using a DeltaVision Restoration Microscopy System (Applied Precision/GE Life Sciences) with an inverted Olympus IX70 microscope with a 20 × oil objective ...
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bioRxiv - Molecular Biology 2024Quote: ... Signals were detected using ECL Prime Western Blotting Detection Reagents (Amersham, Cat# RPN2232) on the Image Quant LAS 4000 system (GE Healthcare). Band images were quantified by Fiji.
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bioRxiv - Molecular Biology 2024Quote: ... The eluate was loaded onto a Superose 6 column (GE Healthcare, Chicago, USA) in protein buffer (20 mM TRIS-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... passed through a 0.45 µM filter and loaded onto a pre-equilibrated HisTrap HP 5ml column (GE Healthcare, IL, US). The column was washed with 50 ml lysis buffer and eluted with buffer containing 200 mM imidazole ...
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bioRxiv - Cancer Biology 2024Quote: ... The peptides were desalted using a homemade C18 microcolumn (SOURCE 15RPC, GE Healthcare) and stored at −30 °C until use.
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bioRxiv - Neuroscience 2024Quote: ... Cell pellet was further resuspended in 30% Percoll (GE Healthcare) and spun at 300g for 15 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... cells were resuspended in 30% Percoll (100% Percoll Plus, 17-5445-02, GE Healthcare) and centrifuged at 300g for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Plates were dried at room temperature for at least 12 hours before images were captured using an Amersham Imager 600 (GE Healthcare UK Limited, Buckinghamshire, UK). Analysis was performed using ImageJ.
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bioRxiv - Microbiology 2024Quote: ... The final step of purification was performed with size-exclusion chromatography on HiLoad 16/60 Superdex 200pg column (GE Healthcare) in 25 mM HEPES buffer pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... The blots were developed using enhanced chemiluminescence solution (GE Healthcare). To estimate the molecular weight of the analyzed proteins PageRuler Prestained Protein Ladder (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The amount of zinc in the oocytes was visualized by a Phosphor Imager (Typhoon FLA 9500; GE Healthcare), and quantified by ImageJ software ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed three times in TBST before bands were visualised using ECL (GE Healthcare), using the Chemidoc gel imaging system (Biorad) ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were then washed in TBST and incubated for 1 hour at room temperature with HRP-conjugated secondary antibody (1:10000 in PBS; GE Healthcare/Biocat) Membranes were washed three times in TBST before bands were visualised using ECL (GE Healthcare) ...
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bioRxiv - Cancer Biology 2024Quote: ... mixed with 25 μl of protein A-Sepharose beads (GE healthcare, catalog no. 17-0469-01). The immunoprecipitated proteins were eluted from protein A-Sepharose beads by boiling with 2x sample buffer and subjected to SDS-PAGE.
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bioRxiv - Cancer Biology 2024Quote: ... Antibody detection was accomplished using Amersham ECL detection reagent (GE healthcare). Membranes were imaged on a BioRad ChemiDoc XRS+ ...
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bioRxiv - Genetics 2024Quote: ... and size-exclusion chromatography (Superdex-200 Increase 10/300, GE Healthcare).
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bioRxiv - Genetics 2024Quote: ... the membrane was submerged in ECL developing solution (GE Healthcare) and the image was captured on X-ray film ...
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bioRxiv - Cell Biology 2024Quote: ... and imaged with the ImageQuant LAS400 Imaging unit (GE Healthcare).
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bioRxiv - Immunology 2024Quote: ... wells were washed and incubated with horseradish peroxidase (HRP)-conjugated antibodies (GE Healthcare) for 3 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated proteins were immobilized on a CAP chip (Biotin CAPture kit, GE Healthcare). As a blocking control anti-LILRB2 antibody ...
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bioRxiv - Immunology 2024Quote: SPR analysis was performed using a BIAcore2000 (GE Healthcare) at 25°C ...
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bioRxiv - Immunology 2024Quote: ... and ImageQuant LAS4000mini (GE Healthcare) were used for detection ...
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bioRxiv - Immunology 2024Quote: ... spleens were removed and meshed through a 70 µm cell strainer in PBS and lymphocytes were isolated by Ficoll-Paque (GE Healthcare, 17-5442-03). Single cell suspensions were washed and stained for flow cytometric analysis ...
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bioRxiv - Microbiology 2024Quote: ... The end-labelled DNA probe was purified from free label by Sephadex G-50 spin columns (GE Healthcare), and incubated with increasing amounts of purified PhoP in a total volume of 10 µl binding mix (50 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... The purified proteins were further dialyzed by using PD10 column (GE Healthcare), and concentrated using Amicon Ultra 30 K (Millipore ...
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bioRxiv - Genomics 2024Quote: ... extension products were separated by electrophoresis on a 10% denaturing PAGE gel and visualized by a Typhoon Phosphorimager (GE Healthcare, Inc).
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was separated on a 5 ml HiTrap TALON crude column (GE Healthcare, Little Chalfont, UK), using washing in lysis buffer supplemented with 10 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: ... the supernatant was incubated with glutathione-sepharose (0.5 ml per liter culture; GE Healthcare 17513201) for 3 h before elution with lysis buffer + 50 mM glutathione (Applichem A2084,0025) ...
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bioRxiv - Neuroscience 2024Quote: All scans were conducted in a 3 Tesla General Electric scanner with an eight-channel head coil (GE MR750; GE Healthcare). Anatomical images were acquired via T1-weighted ...
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bioRxiv - Cell Biology 2024Quote: ... the protein debris was removed by centrifugation (30000×g, 20 min at 4 °C) and the supernatants were applied to His-Trap chelating column (GE Healthcare), washed by refolding buffer and eluted with the same buffer containing 30 mM imidazole ...
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bioRxiv - Bioengineering 2024Quote: ... The concentration of total RNA was evaluated and measured at 260/280nm by spectrophotometer (NanoVue Plus, GE Healthcare). Synthesis of cDNA was performed from 0.5 μg of total RNA ...
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bioRxiv - Biochemistry 2024Quote: Anti-ASCT2 Ab was purified from the anti-sera using HiTrap Protein G HP (GE Healthcare) following the manufacturer’s protocol ...