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Citations for GE Life Sciences :
2001 - 2050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... non-fat dry milk in TBS-T for 1 h at room temperature in a rocking platform: anti-rabbit or anti-mouse IgG-HRP conjugated antibody from sheep (both 1:10,000 NA934 and NA931 GE Healthcare). Proteins were visualized using ECL or SuperSignal West Femto chemiluminescence reagents (Pierce ...
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bioRxiv - Immunology 2024Quote: ... the pellet was resuspended in 5mL of 40% Percoll (GE Healthcare) and the lymphocytes were collected from the pellet after centrifugation at 2,200 g ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... NHR2 was then purified by S-75 size exclusion chromatography (SEC buffer-50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DTT, pH 8) (GE Healthcare Life Sciences) and analyzed using SDS-PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... The antibody binding was visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE healthcare; #RPN2236) and imaged with the ImageQuant LAS400 Imaging unit (GE Healthcare).
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bioRxiv - Bioengineering 2024Quote: ... Unbound oligos were subsequently removed through Ni-NTA purification followed by size exclusion chromatography using a Superdex 200 column (GE Healthcare). The proteins before and after the reaction were analyzed by SDS-PAGE ...
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bioRxiv - Biophysics 2024Quote: ... Deglycosylated proteins were purified to homogeneity by size exclusion chromatography using a HiLoad Superdex 75 PG column (GE Healthcare) equilibrated in Dulbecco’s Phosphate buffered saline (DPBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Images were acquired using a DeltaVision microscope (GE Healthcare, Buckinghamshire, UK) with a 60× or 20× objective lens ...
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bioRxiv - Biophysics 2024Quote: ... After size exclusion chromatography on a Superdex 200 column (GE Healthcare) in 250mM NaCl 10mM Tris pH 8.7 1mM DTT ...
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bioRxiv - Biophysics 2024Quote: ... The cleaved proteins were further purified by cation exchange chromatography using HiTrap SP HP column (GE Healthcare) equilibrated in buffer C ...
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bioRxiv - Biophysics 2024Quote: ... 17 h) and size exclusion chromatography (Superdex 200 10/300 GL (GE Healthcare)) as previously described34.
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bioRxiv - Biochemistry 2024Quote: ... size exclusion chromatography (Superdex 200 pg 16/600 column, 120 ml bed volume, GE Healthcare) was performed as a final purification step ...
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bioRxiv - Biochemistry 2024Quote: 3D-SIM imaging was performed on an OMX V4 Blaze microscope (GE Healthcare) equipped with three PCO.edge sCMOS cameras ...
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bioRxiv - Biochemistry 2024Quote: ... or glutathione agarose (GE Healthcare). Eluted protein was dialyzed against HEPES-buffered saline (HBS ...
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bioRxiv - Bioengineering 2024Quote: ... eluted fractions from the affinity chromatography column were subjected to size exclusion chromatography (SEC) using an Enrich 70 10/300 column (GE Healthcare Biosciences, Uppsala, Sweden), connected to the NGC Chromatography System (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2024Quote: ... The cleared lysates were pelleted at 18,000 rpm over 40 min and the supernatant was incubated with Ni-Sepharose beads (GE Healthcare) for 1 h ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were stained using SYBR Gold and imaged on an Amersham Typhoon RGB instrument (GE Healthcare), Scans were analyzed in ImageQuant IQTL 8.1 ...
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bioRxiv - Biochemistry 2024Quote: ... CezanneCAT was eluted by cleavage of the GST tag with 15 units/ml TEV protease (SigmaT4455-1KU) in PBS buffer overnight at 4°C and desalted using a PD-10 column (GE Healthcare, cat. # 17085101) in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... followed by Mono Q and Superdex200 16/600 (GE Healthcare) size-exclusion chromatography ...
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bioRxiv - Biochemistry 2024Quote: Isothermal titration calorimetry (ITC) experiments were performed on MicroCal PEAQ-ITC (Malvern Panalytical) and MicroCal ITC200 (GE Healthcare), using the 19-injection standard method at 25°C ...
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bioRxiv - Biochemistry 2024Quote: ... Biotinylated-Cdc20WD40 was immobilised onto a SA biosensor chip (GE healthcare) in running buffer (10 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... at 8000 × g then subjected to two rounds of buffer exchange using equimolar Tris-KCl buffer and a 5-mL desalting column (HiTrap Desalting; GE Healthcare) pre-equilibrated with four column volumes of Tris-KCl ...
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Biochemistry 2024Quote: ... Q Sepharose resin purification (GE Healthcare), and subsequently used for the labeling reaction ...
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bioRxiv - Biochemistry 2024Quote: ... The eluents were pooled and directly loaded onto HiTrap Q HP 5 mL anion exchange column (GE Healthcare) pre-equilibrated with buffer D [50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... gels were imaged in a Typhoon imager (GE Healthcare) using excitation and emission wavelengths of 495 nm and 520 nm ...
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bioRxiv - Biochemistry 2024Quote: ... The 6% acrylamide gels were scanned on a Typhoon scanner (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... The resulting lysate was filtered through a 0.2 μm nylon filter and loaded onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences) that had been equilibrated in Buffer A ...
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bioRxiv - Biochemistry 2024Quote: ... the resulting supernatant was adjusted to 500 mM NaCl and 20 mM imidazole and loaded onto a HiTrap Chelating HP column (Amersham Biosciences, GE Healthcare). All purification procedures were carried out at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Radiolabeled PC were analyzed using an FLA-7000 imaging analyzer and quantified using ImageQuant TL version 8.1 (GE Healthcare). For ethanolamine phosphotransferase assay ...
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bioRxiv - Biochemistry 2024Quote: ... before visualization using an Image Quant LAS 4000 (GE healthcare Life Sciences).
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bioRxiv - Biochemistry 2024Quote: SPR analysis was performed using a Biacore 8K system (GE Healthcare). Purified norovirus P-domain from GII.4 and GII.17 strains were immobilized to a CM5 sensor chip using the amine coupling method ...
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bioRxiv - Biochemistry 2024Quote: ... The dialyzed protein was then concentrated using Amicon centrifugal filter units (10,000 MWCO) and further purified by size-exclusion chromatography (Superdex Hiload 75, GE Healthcare) using storage buffer (25 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting protein was then concentrated using Amicon centrifugal filter units (10,000 MWCO) and further purified by size-exclusion chromatography (Superdex Hiload 75, GE Healthcare) using storage buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... monomeric and tetrameric protein were separated and collected by SEC using a Superdex 75 Increase 10/300 GL column (GE Healthcare) and then used directly in fluorescence experiments (SI Appendix ...
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bioRxiv - Biophysics 2024Quote: ... TEV-cleaved protein was then diluted 1:500 in KBT and then loaded onto a HiTrap Q HP column (GE Healthcare) for ion exchange chromatography (IEC ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was loaded onto 1 ml HisTrap HP column (GE Healthcare) on an AKTA fast protein liquid chromatography (FPLC) ...
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bioRxiv - Biophysics 2024Quote: ... We used size exclusion chromatography (SEC) on a Superdex 200 10/300 column (GE Healthcare) to evaluate oligomerization state or collect fractions in either KBT or High-KBT (500 mM KCl ...
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bioRxiv - Biophysics 2024Quote: ... the protein was filtered and loaded onto two connected 5 ml HiTrap Q HP columns (GE Healthcare, 17- 1154-01) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... and exposed in a storage phosphor screen (GE Healthcare) for at least 24 hours ...
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bioRxiv - Biochemistry 2024Quote: ... affinity chromatography followed by size exclusion chromatography (HiLoad Superdex 75 pg or 250 pg; GE Life Sciences). Due to the limited solubility of some of the proteins ...
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bioRxiv - Biochemistry 2024Quote: ... Gel cassettes were imaged in a Typhoon imager (GE Healthcare) with the Phosphor setting ...
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bioRxiv - Biophysics 2024Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap CaptoS 5-ml column at 4° C (GE Healthcare). The column was washed with 10 vol ...
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bioRxiv - Bioengineering 2024Quote: ... Refolded proteins were further purified on a Superdex-200 16/600 column (GE Healthcare) using 20 mM TrisHCl [pH 8.0] ...
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bioRxiv - Biochemistry 2024Quote: ... The dialyzed solution was then loaded on a 5 ml HiTrap SP FF column (GE Healthcare, Pittsburgh, PA) and eluted using a linear gradient of NaCl (20-500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... Purification was performed by HPLC Äkta purifier system (Cytiva, formerly GE Healthcare) with the following method ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was loaded on a 1 ml HisTrap HP column (GE Healthcare, Pittsburgh, PA) and non-specifically bound proteins were removed by washing the column with 20 mM NaPO4 pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a Superdex 75 16/600 (GE Healthcare) size-exclusion chromatography ...
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bioRxiv - Biochemistry 2024Quote: ... Then tag-cleaved proteins was purified with ion exchange chromatography (HiTrap SP FF column (GE Healthcare). Target proteins in the HiTrap SP FF column were eluted by a linear gradient of NaCl and further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were purified from the supernatant using a His-trap affinity column (GE Healthcare) with a stepwise elution method ...
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bioRxiv - Biochemistry 2024Quote: ... Gel-separated radioactive RNA was detected using radioactive films (GE HealthCare) and scanned using a Typhoon scanner (Thermo Scientific) ...