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Citations for GE Life Sciences :
1301 - 1350 of 1897 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... the protein bands on SDS-PAGE gels were scanned and quantified by laser densitometry (Personal Densitometer SI; GE Healthcare, IL). The densities of the Hoc ...
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bioRxiv - Immunology 2022Quote: ... Srt-VSG3 was then separated from remaining contaminants and aggregation products via a gel filtration column (Superdex 200, GE Healthcare) equilibrated in 20 mM HEPES buffer with 150 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... ΔCter and free p85α were further subjected to gel filtration using SuperdexTM 200 10/300 GL Increase from GE healthcare. After gel filtration ...
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bioRxiv - Plant Biology 2019Quote: ... The remaining contaminants were removed by gel filtration chromatography on a Superdex S75 26/600 column (GE Healthcare, Amersham, UK). PL1 was concentrated using a centrifugal concentrator (Vivaspin 20 ...
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bioRxiv - Microbiology 2019Quote: ... Eluates were buffer exchanged into gel filtration buffer (25 mM Tris-Cl, 150 mM NaCl, pH 7.5) via HiPrep 26/10 desalting column (GE Healthcare), followed by overnight incubation with Ulp-1 to cleave off the His6-tagged SUMO moiety ...
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bioRxiv - Microbiology 2019Quote: ... 5-10 µg of proteins were resolved on 4–12% SDS-polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with various antibodies prior to development with ECL prime (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was incubated 2 h on ice then applied onto a Superose 6 Increase 3.2/300 gel filtration column (GE Healthcare) equilibrated in 20 mM Tris-acetate (pH 7.5) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The gels were dried and exposed for 14 days on a Storage Phosphor Screen (GE Healthcare Biosciences, Chicago, IL, USA) and imaged with a Typhoon FLA 7000 (GE Healthcare Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... followed by gel filtration with a Superdex 200 16/600 column (hand poured using prep grade resin from GE Healthcare) in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... The assembly and purity of complexes from peak fractions of the Superdex200 10/300 GL gel filtration column (GE Healthcare) was assessed by SDS-PAGE gel electrophoresis and SimplyBlue SafeStain (Invitrogen ...
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bioRxiv - Zoology 2020Quote: The SDS-PAGE gel was transferred to 0.45µm pore nitrocellulose (NC) membrane (GE Life Sciences #10600003 Protran Premium 0.45 NC) using the AA-Hoefer TE22 Mighty Small Transfer unit at 110V for 90 minutes with water cooling and immersion in an icebath ...
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bioRxiv - Biochemistry 2020Quote: ... and flow-through containing the cleaved SALM3-LRR was collected and gel filtered with Superdex 75 10/300 (GE Healthcare) in 60 mM Tris pH 7.5 ...
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bioRxiv - Physiology 2020Quote: ... YFP-tagged proteins were visualized by scanning the wet PAGE gels with a fluorescence scanner (Typhoon, GE Healthcare, München, Germany). Individual bands were quantified with ImageJ (53).
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bioRxiv - Biochemistry 2020Quote: ... The proteins were concentrated ,applied to gel-filtration using a Superdex 75 prep grade HiLoad 16/60 column (GE Healthcare) equilibrated in buffer K (10 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... MRPP1 and 2 formed a complex of ~150 kDa that was further purified by gel-filtration (Superdex 200, GE Healthcare). His6-Δ95 MRPP3 cDNA lacking the N-terminal disordered region was cloned into pMCSG7 vector and expressed and purified ...
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bioRxiv - Biochemistry 2020Quote: ... DTT or diamide was removed by gel filtration on a Superdex 200 10/300 Increase column (GE Healthcare, Chicago, USA). For isotopically-labeled samples used in nuclear magnetic resonance spectroscopy (NMR ...
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bioRxiv - Molecular Biology 2019Quote: ... Gel scans and fluorescence images were recorded on Typhoon FLA 5100 and Typhoon FLA 9000 Scanners (GE Healthcare Life Sciences). Densitometric quantification of gel bands was performed in Fiji (v ...
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bioRxiv - Biophysics 2021Quote: ... and in-gel fluorescence of the SecY-conjugated ATTO 643 dye was visualized (Amersham Imager 680RGB, GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2020Quote: ... Labeled NBD1 was spun at 4 °C and 16,000 g for 30 minutes and passed through a gel filtration column (Superdex 200 Increase 10/300 GL - GE Healthcare) previously equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Synthetic Biology 2019Quote: ... The HisTrap eluate was subsequently loaded on a Superdex 200 10/300 GL 24 ml gel filtration column (GE Healthcare), and eluted with buffer A plus 250 mM imidazole at a flow rate of 0.75 ml min−1 ...
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bioRxiv - Cell Biology 2021Quote: ... The peak corresponding to diubiquitin was pooled and further purified by gel filtration using a Superdex 75 column (GE Healthcare) equilibrated in 25 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... Reaction products were run on a 6% non-denaturing polyacrylamide gel and quantified using a Typhoon 7000 phosphorimager (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... the cultures were spun down at 12,000 g for 30 min and the supernatants were loaded onto a His-Select Nickel Affinity gel (GE Healthcare). Recombinant PstS proteins were eluted with the elution buffer (500 mM imidazole ...
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bioRxiv - Neuroscience 2021Quote: ... Then the dialysate was filtered and concentrated before a further purification with a Superdex 75 gel filtration column (GE Healthcare) in a buffer of 25 mM Na2HPO4 ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were loaded on a pre-equilibrated Superdex 200 10/300 gel filtration column (GE Healthcare, Chicago, United States) connected to an ÄKTA Purifier 10 system (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... Reaction mixtures (18.5 μl) were resolved on 5% Tris-Glycine native gels and imaged on a Typhoon scanner (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA from WT-GFP and mutant parasites was digested with HindIII and fractionated on a 0.8% agarose gel before blotting onto a nylon membrane (GE Healthcare). A probe was generated from a PCR fragment homologous to the 3’ sequence just outside of the targeted region using the AlkPhos direct labelling kit (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Sec13/31 and/or NHis-Sec13/31 aliquots were thawed and gel-filtrated on a 2.4 mL Superdex200 column (GE Healthcare) mounted on a ÄktaMicro (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... 10% (v/v) glycerol and loaded on a HiLoad 16/10 Superdex 200 prep grade gel filtration column (GE Healthcare) equilibrated in the same buffer ...
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bioRxiv - Genomics 2021Quote: ... The gel was exposed to a phosphor screen and the image was scanned using a Typhoon FLA7000 scanner (GE Healthcare). Gel quantification was performed with the ImageQuant software (GE healthcare).
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bioRxiv - Microbiology 2020Quote: ... and further purified by gel filtration chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL)
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bioRxiv - Microbiology 2021Quote: ... The gel was rinsed with double distilled H2O and scanned at the TAMRA channel on a Typhoon Scanner (GE Healthcare). In an alternative procedure ...
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bioRxiv - Microbiology 2020Quote: ... the harvested protein was concentrated to approximately 2.0 ml and filtered using a Superdex 200 gel filtration column (GE Healthcare) equilibrated with buffer (20 mM Tris-HCl and 200 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... RNA products were resolved on a denaturing sequencing gel and visualized by Typhoon 95000 FLA Imager (GE Healthcare Life Sciences). The RNA sequences used for extending RMP-containing RNA oligonucleotides are ...
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bioRxiv - Immunology 2020Quote: ... to remove the GST tag and further purified by gel filtration (SuperoseTM 6 prep grade XK 16/70; GE Healthcare). To prepare for crystallization trials ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNA isoforms were visualised and quantified using an Amersham Typhoon Gel and Blot Imaging System (GE Healthcare Bio-Sciences AB). The RiboRuler High Range RNA Ladder (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... elution in buffer A plus 350mM imidazole (pH 8) and gel filtration chromatography on a Superdex 200 column (GE Healthcare) in buffer A.
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bioRxiv - Cell Biology 2020Quote: Samples were loaded onto 10% or 6% SDS-PAGE gels and transferred to a 0.45 µm nitrocellulose membrane (Amersham GE Healthcare) overnight at 4°C in Tris-Glycine buffer under constant voltage (30V) ...
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bioRxiv - Immunology 2021Quote: ... Human and mouse GSDMD were further purified by gel filtration chromatography using a Superdex200 (16/60 GL) column (GE Healthcare) in a running buffer containing 20 mM Tris ...
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bioRxiv - Genomics 2021Quote: ... Protein lysates were separated in 12% acrylamide gels and transferred to nitrocellulose blotting membrane (cat.# 10600004-GE Healthcare Life Sciences). The membrane was blocked with 5% skim milk in TBS-T (20 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... equal amounts of protein (30 µg) were run on homemade 10% polyacrylamide gels and transferred on nitrocellulose membranes (GE Healthcare). Membranes were probed with primary antibodies (listed in the table below) ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was then exposed overnight on a phosphor screen and visualized with a Typhoon FLA9500 (GE Healthcare Life Sciences). For SELEX assays ...
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bioRxiv - Biochemistry 2022Quote: ... Small RNAs were separated on a 15% sequencing gel and transferred to Amersham Hybond-N+ membranes (GE Healthcare, cat# RPN303B) using a Trans-blot SD semi-dry electrophoretic transfer cell (Bio-Rad ...
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bioRxiv - Biophysics 2022Quote: ... Excess unreacted BADAN was removed by gel filtration by passing the reaction solution through a PD-10 column (GE Healthcare). Labeling efficiency was measured by UV-visible spectroscopy based on extinction coefficients of SLO-1 (ε280 = 132 mM-1 cm-1 ...
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bioRxiv - Biophysics 2022Quote: ... The concentrated protein was filtered through a 0.2 µm filter and injected on a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: Samples of the different complexes were prepared at 10 μM and 100 μl were injected onto a 2.4 mL Superdex 200 Increase gel filtration column (GE Healthcare) pre-equilibrated in 100 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... Afterwards the lysates were subjected to SDS-polyacrylamide gel electrophoresis using an 8% polyacrylamide gel and then electroblotted onto a nitrocellulose membrane with a pore size of 0.45 μm (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... Further purification was performed in column buffer (25 mM Tris HCl pH 8.0 and 150 mM NaCl) using HiLoad Superdex 200 gel-filtration chromatography (GE Healthcare). The peak fractions were collected ...
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bioRxiv - Biophysics 2022Quote: ... SEC–MALS was performed using 20 mM Tris pH 7.5 and 150 mM NaCl buffer equilibrated analytical Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare) on a Shimadzu HPLC ...