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Citations for GE Life Sciences :
1501 - 1550 of 3483 citations for 7 8 Dihydro 6H 1 6naphthyridin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The resultant supernatant was filtered using a 0.45 µm filter and the protein was subsequently purified by IMAC on a 5 ml HP HisTrap column (GE Healthcare) according to manufacturer’s information ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was then dialyzed against 10 mM Tris HCl pH 8.0 and loaded on MonoQ 5/15 column (GE Healthcare) to be eluted using a step gradient of 240 mM and 400 mM NaCl in the same buffer.
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was centrifuged at 40,000 rcf for 1 h and the supernatant was filtered through a 0.45 μM syringe filter (Chromafil GF/PET 45, MachereyNagel) and passed through a HiTrap SP HP (5 mL, GE Healthcare) column connected to an NGC FPLC system (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700 x g for 12 minutes ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a an isotonic 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700x g for 12 minutes ...
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bioRxiv - Biophysics 2023Quote: ... followed by centrifugation at 20,000 x g where the clear lysate was subsequently loaded onto a 5 mL Ni-NTA Sepharose column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was passed through a 0.22 µm filter and loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with 25 ml of lysis buffer containing 30 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: Elute from HisTrap consisting of the respective protein fractions were centrifuged at 39,000 g for 15 minutes and the supernatant was loaded on a 5 ml StrepTrap HP column (GE Healthcare). The binding buffer used was 150 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was diluted 5-fold to contain 100 mM NaCl and purified over SP-Sepharose (HiPrep SPFF, GE Healthcare) using a linear gradient of buffer containing 2 M NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was loaded in an ÄKTA Pure purification system (Cytiva) onto a 5 ml HiTrap Chelating HP (GE Healthcare) charged with 0.1 mM NiCl2 and equilibrated with wash buffer (50 mM K-HEPES pH7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
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bioRxiv - Biophysics 2023Quote: ... HRP-secondary antibodies were incubated for 1-2 hours at room temperature in 5% milk and ECL were performed using the Amersham ECL Western Blotting Detection Reagents (Cat.no. RPN2106 from GE Healthcare). Detection was done using CL-Xposure films (Cat.no ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied over 20 min (at 5 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... and lysates were clarified (16,000 x g for 45 min at 4 °C) before being loaded onto a 5 mL HisTrap column (GE Healthcare). This was followed by one column volume (c.v. ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a MF-Millipore Membrane Filter (0.45 µm pore size) then loaded on a 5 mL His-Trap HP column (GE Healthcare) pre-equilibrated with buffer A and CSE was eluted with a gradient from 20 mM to 250 mM imidazole in buffer A ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was loaded in an ÄKTA Pure purification system (Cytiva) onto a 5 ml HiTrap Chelating HP (GE Healthcare) charged with 0.1 mM NiCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged at 16,000 g for 20 min at 4°C and then injected into Superose 6 5/150 increase column (GE Healthcare) pre-equilibrated with analytical size-exclusion chromatography buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... the Ni-NTA eluate was applied to size exclusion chromatography on a Superose 6 Increase 5/150 column (GE Healthcare) in basic buffer supplemented with 0.003 % LMNG and 0.0003 % CHS.
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bioRxiv - Biochemistry 2023Quote: ... pH 8.0) and further purified via size exclusion chromatography (SEC) in an S75 5/150 GL increase column (GE Healthcare). Protein collected from SEC was normalized to 1 μM where possible.
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bioRxiv - Molecular Biology 2023Quote: ... Then the samples were subjected to electrophoresis through 5% native polyacrylamide gel in 0.5x TBE buffer and imaged with 9410 Typhoon variable mode imager (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... the KhpA and KhpB-ΔN were secondly purified by cation-exchange chromatography (HiTrap SP 5 ml, GE Healthcare Life Science) followed by SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: ... The lysate was clarified by centrifugation at 47,000 x g for 45min and applied to 5 ml His-Trap columns (GE Healthcare). After washing with a buffer excluding urea ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was filtered in 0.45 µm filter and loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... HRP-secondary antibodies were incubated for 1-2 hours at room temperature in 5% milk and ECL were performed using the Amersham ECL Western Blotting Detection Reagents (Cat.no. RPN2106 from GE Healthcare). Detection was done using the Chemidoc XRS imaging system (Bio-Rad)..
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were developed using 1:1 Amersham ECL western blotting reagent (GE Healthcare) to SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Capto Core 700 beads (GE Healthcare; ~1:1 resin volume to sample volume) were used to remove residual dyes and enzymes from the solution of labeled virus ...
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bioRxiv - Biochemistry 2021Quote: ... a 1:1 mixture of 50 mg/mL Sera-Mag SpeedBeads (GE Healthcare) and 50 mg/mL Sera-Mag SpeedBeads (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-FBH1[1-250] in the pGEX6P-1 vector (Amersham/GE Healthcare/Cytiva) was introduced into BL21:DE3 Rosetta cells (Novagen) ...
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bioRxiv - Biophysics 2021Quote: ... 50 µL of 6 µM Cdt1 or Cdt1Uniform was prepared in Protein Buffer and then loaded and run on a Superose 5/150 GL sizing column (GE Healthcare) pre-equilibrated and run in Protein Buffer ...
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bioRxiv - Biophysics 2021Quote: ... the supernatant was filtered using a 0.45 µm filter and then loaded onto a 5 ml HisTrap™ HP nickel sepharose column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Cell Biology 2020Quote: GST-tagged proteins (Retromer, SNX3, WASHC2C-5 and WASHC2C-21) were purified by incubating clarified lysate with glutathione (GSH) Sepharose 4B (GE Healthcare) beads ...
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bioRxiv - Cell Biology 2019Quote: ... Lysate was cleared with centrifugation and loaded to HisTrap FF crude 5 ml Ni-NTA column (GE healthcare, #17-5286-01). Column was washed with 10 column volume of lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 250 μg of MCF10A cell lysate and 5 μg of individual GST-tagged recombinant proteins were mixed and incubated with glutathione Sepharose 4b beads (GE Healthcare) at 4°C overnight ...
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bioRxiv - Microbiology 2019Quote: ... experiments coupled to multi-angle laser light scattering (MALS) and refractometry (RI) were performed on a Superdex S200 5/150 GL increase column (GE Healthcare). 25 μl of AdhE mutant protein were injected at a concentration of 10 mg.ml−1 in buffer 0.05M Tris HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 or 10 µg of the purified RNA was resolved on formaldehyde agarose gels and transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the dialyzed sample was loaded on an anion exchange HiTrap Q HP 5 mL column (GE healthcare life sciences, Boston, Massachusetts) pre-equilibrated with 20 mM bis-Tris pH 5.5 ...
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bioRxiv - Biochemistry 2022Quote: The interaction of N proteins and 14-3-3γ was analyzed using a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate on a Prostar 335 chromatographic system (Varian ...
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bioRxiv - Biophysics 2022Quote: ... Cell disruption was achieved by sonication (Branson Sonifier SFX250) and the soluble fraction was subjected to immobilized metal affinity purification using a 5 ml Ni-NTA column (GE healthcare) connected to a FPLC system (GE healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... The eluted fractions were concentrated to 5 ml and loaded onto a HiLoad 26/600 Superdex 200 prep grade (GE Healthcare) equilibrated with the SEC buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Cell Biology 2019Quote: Cells were fixed with 4% paraformaldehyde for 5 min and images were captured at room temperature on a DeltaVision fluorescence microscope (Applied Precision) installed with softWoRx version 5.5 housed in the Micron Oxford essentially as described [13] ...
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bioRxiv - Bioengineering 2019Quote: ... Lysate was clarified by centrifugation at 35,000g and purified by immobilized metal affinity chromatography (IMAC) on a 5 mL Fast Flow Chelating Sepharose column (GE Life Sciences) with an elution gradient from 0 to 200 mM imidazole over 120 mL ...
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bioRxiv - Neuroscience 2019Quote: ... Labeled proteins were separated from the free fluorophore using a NAP-5 Sephadex G-25 desalting columns in labeling buffer (GE Healthcare). The labeling efficiency was determined using an extinction coefficient of 5400 M-1cm-1 at 335 nm for IAEDANS and the protein concentration was determined by Bradford assay using BSA as a standard ...
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bioRxiv - Biochemistry 2020Quote: ... 1.5 μM PCNA trimer and 20 μM TTP was loaded onto a Superdex 200 increase 3.2/300 column (GE Life Sciences) equilibrated with a buffer comprising [25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2019Quote: ... as described previously for the non-fluorescent version (39): i) affinity purification of filtered supernatant with 5 ml HisTrap FF column (GE Healthcare) and step elution with 300 mM imidazole ...
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bioRxiv - Biophysics 2019Quote: ... The protein solution was then syringe filtered using a 0.22 µm PES syringe filter and 5 mL were injected onto a Highload Superdex200 16/600 size exclusion column (GE Healthcare). During size exclusion chromatography ...
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bioRxiv - Biophysics 2019Quote: ... Rab8-His6 proteins were purified from the supernatant by nickel affinity chromatography (5 ml HiTrap™ Chelating HP column, GE Healthcare). Unspecifically bound proteins were removed by a step wash with 5% buffer B (50 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... The protein solution was then syringe filtered using a 0.22 μm PES syringe filter and 5 mL were injected onto a Highload Superdex200 16/600 size exclusion column (GE Healthcare). During size exclusion chromatography ...
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bioRxiv - Molecular Biology 2019Quote: ... The reaction was heat-inactivated for 5 min at 95°C and the free nucleotides were removed on a Sephadex G-25 column (GE Healthcare). The oligo was annealed to a 1.5x excess of the unlabelled complementary strand by heating for 5 min to 80°C and slowly cooling to RT in annealing buffer (50 mM Hepes ...