Labshake search
Citations for GE Life Sciences :
1301 - 1350 of 3582 citations for 7 8 Dihydro 6H 1 6naphthyridin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: ... The protein was then purified by anion exchange chromatography on a MonoQ 5/50 GL column (GE Healthcare) using buffers AEX-A and AEX-B with a gradient of 7.5-50% B over 20 CV at a flow rate of 1 ml/min ...
-
bioRxiv - Genomics 2021Quote: ... 2 mM MgCl2) and then loaded onto two tandem 5 mL heparin HP pre-packed columns (GE Healthcare). Sen1 was eluted using a linear gradient from 20% to 100% of buffer B2 containing 50 mM HEPES-NaOH ...
-
bioRxiv - Biophysics 2020Quote: ... Fractions containing Pol I were then loaded onto a 5-mM HiTrap Heparin HP column (GE Life Sciences) equilibrated in Heparin BufferA(50mMTris-HCl ...
-
bioRxiv - Genetics 2019Quote: The clarified lysate containing mZCWPW1 was then loaded onto a 5 mL His-Trap HP column (GE Healthcare) using NGCTM chromatography system (BioRad) ...
-
bioRxiv - Cell Biology 2021Quote: Approximately 5 μg of GST or GST-tagged protein fragments were incubated in Glutathione-Sepharose beads (GE Healthcare) in GST-lysis buffer at 4°C overnight ...
-
bioRxiv - Microbiology 2022Quote: ... Strep-tagged proteins were affinity-purified on an ÄKTA Purifier using 5 ml StrepTrap HP columns (GE Healthcare) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... Recombinant Sau-MenD was purified at room temperature by IMAC with 5 mL HisTrap HP columns (GE Healthcare) and an imidazole gradient of 20–500 mM over 50 mL ...
-
bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was added to a 5 mL Ni-Sepharose fast-flow resin (GE Healthcare Life Sciences) pre-equilibrated with buffer A at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... Chemiluminescence was detected after 5 min of incubation with ECL™ Select Western Blotting Detection Reagent (GE Healthcare) in a ChemiDoc chemiluminescence detector (BioRad) ...
-
bioRxiv - Biophysics 2024Quote: ... a 2-elution step Ni2+-affinity chromatography was carried out using a Histrap HP 5 ml (GE Healthcare) on an FPLC apparatus (ÄKTA Pure ...
-
bioRxiv - Biochemistry 2024Quote: ... The Spt4/5 heterodimer was further purified by size exclusion chromatography (Superdex 75 10/300 GL, GE Healthcare) through isocratic elution in low salt buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... We then applied the supernatant onto two tandem 5 mL HiTrap Q HP anion exchange columns (GE Healthcare) pre-equilibrated with 20 mM Tris pH 8.0 ...
-
bioRxiv - Immunology 2024Quote: ... blots were incubated at room temperature for 5 min in the dark with ECL detection reagent (GE Healthcare), made up in accordance with manufacturers’ instructions ...
-
bioRxiv - Biophysics 2024Quote: The PCR product was purified via ion exchange chromatography using a 5 ml HiTrap Q column (GE Healthcare). The column was equilibrated in Buffer A (25 mM HEPES 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Then the solution was injected onto a prewashed SP Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL). The column was washed with 20 CV of Buffer D (20 mM HEPES-KOH pH 7.5 ...
-
bioRxiv - Genetics 2023Quote: ... for 45 min and soluble extract was applied to a 5 ml HisTrap FF column (GE healthcare Inc.) using a GE AKTA Avant 25 FPLC system ...
-
bioRxiv - Microbiology 2023Quote: ... proteins were separated by immobilized-metal affinity chromatography (IMAC) on a 5 mL HisTrap HP column (GE Healthcare), previously washed with ddH2O and equilibrated with buffer B (25 mM HEPES/NaOH pH 7.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... Samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
-
bioRxiv - Biophysics 2022Quote: ... with a cut-off of 5 kDa and injected into HiLoad Superdex 200 prep grade chromatography (GE Healthcare) equilibrated with buffer C (20 mM Hepes-NaOH ...
-
bioRxiv - Microbiology 2023Quote: ... The cell-free lysate was applied to a 5 ml His Trap™ HP column (GE Healthcare, USA) equilibrated in the same buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was then transferred to a 5 mL tube containing 300 μL CaM-Sepharose resin (GE Healthcare) equilibrated in 50 mM HEPES (pH 7.2 ...
-
bioRxiv - Biochemistry 2023Quote: ... Aurora A protein was thawed and buffer exchanged in the experimental buffer using NAP-5 Columns (GE Healthcare). Experiments typically involved titrating 25 µM of protein in the sample cell with 300 µM of compound in the syringe ...
-
bioRxiv - Biochemistry 2023Quote: The ammonium sulfate treated hMDH preps were loaded onto a 5 mL HiTrap Butyl HP column (GE Healthcare). An inverse linear gradient of ammonium sulfate from 1.5 M to 0 M was used to purify hMDH where it eluted at a conductivity of 135 mS/cm ...
-
bioRxiv - Biophysics 2023Quote: ... centrifuged and applied to a cation exchange chromatography column (5 mL HiTrap SP HP, GE Healthcare Life Science) equilibrated with 25 mM sodium phosphate ...
-
bioRxiv - Biochemistry 2023Quote: ... a second nickel-affinity chromatography was performed (His Trap, 5 mL, GE Healthcare; equilibrated in 10% HisB buffer). Sample was loaded and unbound protein further washed out with 10% HisB ...
-
bioRxiv - Cell Biology 2024Quote: Time-lapse images were collected at 37°C with 5% CO2 using a DeltaVision ELITE microscope (Applied Precision). We used an apochromatic 100x objective lens (Olympus ...
-
bioRxiv - Biochemistry 2024Quote: ... TEV protease and non-cleaved protein was removed by IMAC (HisTrap FF Crude column, 5 ml, GE Healthcare) with a linear gradient of imidazole (0-500 mM) ...
-
bioRxiv - Biochemistry 2024Quote: ... The eluents were pooled and directly loaded onto HiTrap Q HP 5 mL anion exchange column (GE Healthcare) pre-equilibrated with buffer D [50 mM Tris-HCl (pH 7.5) ...
-
bioRxiv - Biochemistry 2024Quote: ... The dialyzed solution was then loaded on a 5 ml HiTrap SP FF column (GE Healthcare, Pittsburgh, PA) and eluted using a linear gradient of NaCl (20-500 mM) ...
-
bioRxiv - Biochemistry 2024Quote: ... Excess HaloTag ligand was then removed by purification on desalting NAP-5 or PD-10 columns (GE Healthcare). To generate the HRP-his6Halo-hPKR-N conjugate ...
-
bioRxiv - Biophysics 2019Quote: ... 100-nm LUVs of 1 mM DMPC:DMPG (1:1) were immobilized on an L1 sensor chip (GE Healthcare) in HBS ...
-
bioRxiv - Microbiology 2019Quote: ... at a dilution of 1:10000 for 1 hour (GE Healthcare). Proteins were detected using SuperSignal West Femto substrate (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein A and Protein G beads mix (1:1) (GE Healthcare) were washed with HEMG100 ...
-
bioRxiv - Immunology 2019Quote: ... 1-360) were cloned into vector pGEX-6P-1 (GE Healthcare), facilitating bacterial expression as a GST-fusion ...
-
bioRxiv - Microbiology 2022Quote: ... at a dilution of 1:10,000 for 1 h (GE Healthcare). Proteins were detected using SuperSignal West Femto substrate (Thermo Fisher ...
-
bioRxiv - Microbiology 2020Quote: ... coli BL21(DE3) and were isolated under native purification conditions using a 5 HisTrap High Performance Column (GE Healthcare) with imidazole gradient (10-250 mM ...
-
bioRxiv - Neuroscience 2021Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap SP HP 5 ml column (GE Healthcare) for cation exchange ...
-
bioRxiv - Molecular Biology 2021Quote: ... The protein fraction eluted with 250 mM imidazole was purified further by MonoQ 5/50 GL column (GE Healthcare) at pH 8.0 ...
-
bioRxiv - Cell Biology 2022Quote: ... Filtered supernatant was loaded into a 5 mL Histrap column (Cytiva) connected to an FPLC AKTAxpress system (GE Healthcare). After affinity binding ...
-
bioRxiv - Neuroscience 2021Quote: ... They were maintained within 37°C incubators at 5% CO2 in DMEM + high glucose (GE Healthcare Bio-Science, SH30022FS) supplemented with 9.09% fetal bovine serum (50 mL added to 500 mL DMEM ...
-
bioRxiv - Molecular Biology 2020Quote: ... Sucrose gradient fractions containing ODA complexes were further purified over a MonoQ 5/50 anion exchange column (GE Healthcare) to separate out other axonemal dynein species ...
-
bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
-
bioRxiv - Molecular Biology 2019Quote: ... XPG and XPA containing lysate was applied onto GE HisTrap HP 5 mL (GE Healthcare, Little Chalfont, United Kingdom) equilibrated in lysis buffer (in case of XPA all downstream steps were performed in the presence of 5 mM β-mercaptoethanol and 10 μM ZnCl2 instead of 1 mM DTT) ...
-
bioRxiv - Molecular Biology 2019Quote: ... The supernatant was filtered (0.45 µm pore) and loaded onto a 5 ml HisTrap FF column (GE Healthcare, USA). After extensively washing with buffer NA supplemented with 35 mM imidazole ...
-
bioRxiv - Molecular Biology 2019Quote: Clarified extracts containing hexahistidine fusion proteins were passed through a 5 mL Ni-Sepharose (GE Healthcare #17-5268-01) packed column at 0.5 mL/min using an ÄKTAprime Plus chromatography system ...
-
bioRxiv - Molecular Biology 2019Quote: ... Clarified extracts containing GST fusion proteins were passed through a 5 mL GSTrap column (GE Healthcare #17-5131-02) at 0.1 mL/min using an ÄKTAprime Plus chromatography system (GE Healthcare) ...
-
bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B and the cleaved topo VI collected in the flow through ...
-
bioRxiv - Biochemistry 2021Quote: AsLMO10A and AsLPMO10B were purified by anion exchange chromatography using a 5 mL HiTrap DEAE FF column (GE Healthcare) followed by hydrophobic interaction chromatography (HIC ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was additionally purified by ion-exchange chromatography on a “Mono S 5/50 GL” column (GE healthcare) at 0-100% B gradient over 15 column volumes at 0.5 ml/min flow rate using 20 mM sodium phosphate buffer (pH 6.0 at 25 °C ...
-
bioRxiv - Biochemistry 2020Quote: ... The sample was diluted to 70 mM NaCl and injected into an HiTrap Heparin HP 5 ml (GE Healthcare) column equilibrated with 20 mM HEPES pH 7.9 ...