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Citations for GE Life Sciences :
1551 - 1600 of 3483 citations for 7 8 Dihydro 6H 1 6naphthyridin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... The homogenate was centrifuged as before and the supernatant was purified on an AKTA Pure FPLC equipped with a HiTrap Q 5 mL anion exchange column (GE Healthcare). Solvent A was 20 mM tris pH 8.5 ...
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bioRxiv - Bioengineering 2020Quote: ... and the supernatant was purified using an AKTA Pure FPLC equipped with a HisTrap FF crude 5 mL column (GE Healthcare). Solvent A was 20 mM tris pH 7.5 ...
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bioRxiv - Bioengineering 2019Quote: Cell-free supernatants were prepared by centrifugation and directly loaded (100 mL) onto a 5 mL HiTrap MabSelect column (GE Healthcare). After washing the column with binding buffer (20 mM sodium phosphate ...
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bioRxiv - Cancer Biology 2019Quote: ... All fractions identified to contain cleaved RNF114 were concentrated to a volume of 5 mL and loaded onto a HiLoad 16/60 Superdex 75 gel filtration column (GE Healthcare) that had been pre-equilibrated with either crystallography buffer ...
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bioRxiv - Cell Biology 2019Quote: ... The reaction was stopped on ice and labeled polyP300-AF647 was separated from free dye and unlabeled polyP via a NAP-5 column (GE Healthcare) that was equilibrated with 40 mM KPi ...
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bioRxiv - Immunology 2019Quote: ... the cell suspension diluted in 1 mL of complete L15 medium was carefully layered on top of 5 mL of Percoll gradient (L15/10 mM HEPES/20% Percoll (GE Healthcare) and centrifuged at 400 g for 9 min ...
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bioRxiv - Molecular Biology 2019Quote: ... The protein was then concentrated and subjected to size-exclusion chromatography on a Superose 6 5/150 column (GE Healthcare, Germany) in plunging buffer (20 mM TRIS-HCl pH 7.5 ...
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bioRxiv - Genomics 2019Quote: ... cell lysates were treated with 5 units RNase I (ART-Seq) per OD260 and monosomes were purified in MicroSpin S-400 columns (GE Healthcare). For both RPF- and RNA-seq ...
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bioRxiv - Biochemistry 2020Quote: ... Tag and TEV protease removal was achieved by re-loading the dialyzed sample onto the 5 mL HisTrap Excel column (GE Healthcare), and collecting the unbound tagless sample ...
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bioRxiv - Biochemistry 2020Quote: ... The protein of interest (NT-mini or NT*-mini) was then eluted into a 5 ml Ni-IMAC HisTrap Excel column (GE Healthcare) by further increasing the NaCl concentration to 600 mM (buffer NT.B) ...
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bioRxiv - Neuroscience 2020Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at -80°C until use ...
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bioRxiv - Biophysics 2020Quote: ... centrifuged at 14000g and 4 °C for 5 min and the excess of ammonium sulfate was removed by running the supernatant through a NAP-25 column (GE Healthcare). The tAv was purified using HiTrap chelating column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: Fifty milliliters of the supernatant was loaded onto a 50 mL superloop (Amersham Biosciences) and bound to a 5 mL heparin column (HiTrap Heparin HP 1X5 mL, GE Healthcare) by fast protein liquid chromatography (ÄKTApurifier ...
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bioRxiv - Biochemistry 2020Quote: ... The cleaved protein was concentrated to 5 ml and loaded in a HiLoad 16/600 Superdex 75pg gel-filtration column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Cleaved protein was passed through a 5 ml HisTrapFF column and further purified by size-exclusion chromatography (S200 16/60 column; GE Healthcare) in buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Soluble RNA was separated from solid acrylamide by filtration through 0.22 micron filters and then manually applied to a 5 mL HiTrapQ column (GE Healthcare # 29051325) that had been equilibrated in buffer D (300 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) containing 5% skim milk (Meiji) and subjected to primary antibody for 2 h and HRP-conjugated secondary antibody (GE Healthcare) for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were clarified by centrifugation (20 minutes at 18,000x g) and bound to a 5 mL HisTrap™ FF Crude column (GE Healthcare) and eluted with 50-75 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... 4°C for 30 minutes and loaded onto a 5 ml HighTrap MBP column (GE Healtcare) using an Äkta-Pure system (GE Healthcare). The column was washed with lysis buffer and protein eluted with 10 column volumes MBP-elution buffer (100 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biophysics 2021Quote: ... The dialyzed media was filtered again and 5 mM imidazole was added to the media before loading onto a Histrap column (GE healthcare). The protein was eluted using a linear imidazole gradient (20 to 500mM ...
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bioRxiv - Molecular Biology 2020Quote: ... Pyo S8 DNase domain was separated from its cognate immunity protein by loading the sample onto a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated with denaturing buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Stained plates were washed 3 times in dH2O for 5 minutes at room temperature and air dried before imaging using the Imagescanner III (GE Healthcare). Growth was determined by percentage area of the plate covered by cells using ImageJ v1.52a ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation (40000 g for 30 min) and the supernatant applied onto a 5 ml StrepTrap column (GE Healthcare). After washing with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: The oligomeric state of proteins as well as protein-protein and protein-tRNA interactions were analyzed by loading 50 μl samples on a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate using a Varian ProStar 335 system (Varian Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... 10.2 µM protein was incubated with 100 µM probe and 0.5 mM TCEP for ∼20 h at 4 °C and free probe was removed by desalting using a 5 mL HiTrap desalting column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... concentrated in a vivaspin 100,000 MWCO and loaded onto a Superose 6 5/150 size exclusion chromatography column (GE Healthcare Lifescience) to remove thrombin and exchange the buffer to either 20 mm ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 mM EDTA) before further purification by ion-exchange on a MonoQ 5/50 Gl column (GE Healthcare Pte Ltd Singapore) to remove traces of vector and recombination products ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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bioRxiv - Plant Biology 2020Quote: ... Each Z-section image is based on 15 images generated from 3 different angles and 5 different SIM patterns and reconstructed using SOFTWORX software (Applied Precision).
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bioRxiv - Immunology 2020Quote: ... cells were fed with 5% of the starting volume with Cell Boost7a and 0.5% Cell Boost7b (Hyclone GE Healthcare Life Sciences). Cell counts and glucose was measured as previously described,70 On day 8 ...
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bioRxiv - Neuroscience 2019Quote: ... the protein was eluted using 400 mM Imidazole (5 CV) and loaded onto a HiLoad 16/600 Superdex 200 pg size exclusion column (GE Healthcare). The main peak was collected ...
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bioRxiv - Neuroscience 2019Quote: ... The mixture was then purified via reverse Ni-NTA and the buffer was changed to NMR buffer (see above) using either a NAP-5 desalting column (GE Healthcare) or by S200a size exclusion chromatography.
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bioRxiv - Physiology 2021Quote: ... guinea pigs were anesthetized (4-5% isoflurane with 2L/min O2) and placed on a micro-PET scanner (GE eXplore Vista, GE Healthcare) fitted with a nose cone to inhale 2.5-3% isoflurane with 1L/min O2 for maintenance of anesthesia ...
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bioRxiv - Molecular Biology 2020Quote: ... divided into aliquots of 10 mL and each aliquot was added on top of a 5 mL of Ficoll-Paque Premium (GE Healthcare) in 15 mL sterile Falcon tubes ...
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bioRxiv - Microbiology 2020Quote: ... VHH containing fractions were pooled and concentrated and the elution buffer was exchanged with PBS with a Vivaspin column (5 kDa cutoff, GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The reaction mixture containing the protein–DNA complex were resolved on a 5% native PAGE gel on ice and transferred to a nylon membrane (Amersham Hybond-XL, GE healthcare) using 0.5 X TBE ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK-293T (ATCC CRL-3216) cell lines used in this study were cultured at 37°C in 5% CO2 in high-glucose DMEM (GE Healthcare) supplemented with 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were exposed for 10-30 s (in the case of GFP-Plk4) and for 3–5 min (in the case of actin) on a Amersham Imager 680 (GE Healthcare) and images were acquired with its CCD camera.
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bioRxiv - Biophysics 2021Quote: ... The suspension was then centrifuged at 37,500 g for 30 min and the supernatant incubated with 5 mL GFP nanobody-coupled CNBr-activated Sepharose resin (GE Healthcare) for 2 hrs (Kubala et al. ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM NaCl and 10 mM β-ME and loaded onto a 5 ml HiTrap SP anion exchange column (GE Healthcare). The binary complex ScNatC was then eluted using a salt gradient (50–750 mM NaCl) ...
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bioRxiv - Biophysics 2021Quote: ... Peak fractions were pooled, diluted ~5 fold with buffer 0 (20 mM HEPES, 2 mM DTT) and loaded onto a Q column (GE Healthcare). Rad33 was then eluted from the Q column using a linear gradient of Q buffer A (20 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was loaded onto either a 1-ml Mono Q (ParAF proteins and ParBF1-42-mCherry-EcoRIE111Q) or Mono S (other ParBF proteins) 5/50 GL ion exchange column (GE Healthcare) pre-equilibrated with Mono Q/S-buffer containing a NaCl concentration to match the conductivity of the sample ...
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bioRxiv - Biochemistry 2022Quote: ... of GlOCPX or SynOCP1 samples (5 mg/ml in 50 μl) was performed on a Superdex 200 Increase 10/300 column (GE Healthcare) connected to a Prostar 335 detector (Varian ...
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bioRxiv - Biochemistry 2022Quote: ... Individual reactions were transferred to a 96-well plate and analysed using a Superdex 200 Increase 5/150 GL gel filtration column (GE Healthcare) attached to an Agilent 1200 Series Gradient HPLC system ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were then washed with TBS-T (3x, 5 min each) and incubated with horse radish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare). Following another wash step in TBS-T ...
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bioRxiv - Biochemistry 2022Quote: ... The separated species were concentrated to 1.3 - 2.5 mg/ml and 200 µl of each were applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with SEC buffer and connected to a miniDAWN TREOS II triple-angle light scattering detector (Wyatt Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... immobilized protein was eluted using wash buffer and further loaded onto an ion-exchange column (MonoQ 5/50 GL column, GE Healthcare) to achieve highly pure PBAF complex ...
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bioRxiv - Neuroscience 2022Quote: ... TE = 20 ms) were acquired both before and 5 min after intravenous injection of 100µl gadolinium-DTPA (Omniscan®, GE Healthcare). The matrix size and field of view were 256 x 256 and 3.5 cm x 3.5 cm ...
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bioRxiv - Immunology 2022Quote: ... The cell suspension was centrifuged at 1,200 rpm at 4°C for 6 minutes and the pellet was resuspended in 5 ml of 40% Percoll (GE Healthcare) in PBS and transferred to a 15 ml tube ...
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bioRxiv - Microbiology 2022Quote: ... The protein was then concentrated to a final volume of 500 μl using Vivaspin 20 with a molecular weight cut off (MWCO) of 5 kDa (GE Healthcare). The sample was loaded on a Superose6 Increase 10/300 GL size exclusion column from GE Healthcare that had been equilibrated with size exclusion buffer (for INpro-28R ...