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Citations for GE Life Sciences :
1451 - 1500 of 3483 citations for 7 8 Dihydro 6H 1 6naphthyridin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered through a 0.45-μm membrane and loaded onto a HisTrap FF 5 mL column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cooled down on ice and ultracentrifugated at 160,000xg for 1hr at 4°C and loaded onto a 5 mL Protein A (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing MBP-AIM2 were pooled and purified by ion-exchange chromatography using 5 mL HiTrap-SP column (GE Healthcare) equilibrated in 20 mM HEPES ...
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OxoScan-MS: Oxonium ion scanning mass spectrometry facilitates plasma glycoproteomics in large scalebioRxiv - Systems Biology 2022Quote: ... IgG was isolated from 5 μl of serum using 30 μl of Protein A Sepharose (GE Healthcare, Eindhoven, The Netherlands). Sample mixtures were incubated under agitation at 650 rpm for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... All immunoblots were visualized with ECL Plus Detection Reagent and scanned with a Typhoon 5 Variable Mode Imager (GE Healthcare). Results are expressed as means ± SEM and expressed relative to the “no ligand” condition.
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bioRxiv - Neuroscience 2022Quote: ... All immunoblots were visualized with ECL Plus Detection Reagent and scanned with a Typhoon 5 Variable Mode Imager (GE Healthcare). Results are means ± SEM from 5-6 mice per genotype and expressed relative to TrkAWT mice.
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bioRxiv - Immunology 2022Quote: ... The lysed cells were centrifuged and only the supernatant with the soluble proteins was then further filtered with 0.45 µm filters and loaded onto two connecting 5 mL HisTrap IMAC FF columns (GE Healthcare) which were pre-equilibrated with 50mM phosphate buffer with 500 mM NaCl and 20 mM imidazole at pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... to remove cell debris the supernatant was filtered through a 0.45-μm pore size filter and then loaded onto a 5-ml Ni-HiTrap column (GE Healthcare) that had been pre-equilibrated in the same lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was cleared by centrifugation at 100,000 x g for 45 min and loaded onto a 5 mL HisTrap HP column (GE Healthcare). The column was washed extensively with NiA buffer ...
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bioRxiv - Microbiology 2019Quote: ... 5-10 µg of proteins were resolved on 4–12% SDS-polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with various antibodies prior to development with ECL prime (GE Healthcare) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plates were incubated at 37° C and imaged after 20 h with an Amersham Typhoon 5 Biomolecular Imager (GE Healthcare) using the following settings ...
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bioRxiv - Biochemistry 2019Quote: ... the filtrate was loaded on an anion exchange HiTrap Q HP 5 ml column (GE healthcare life sciences, Boston, Massachusetts), at a flow rate of 1 ml/min ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1bioRxiv - Biochemistry 2019Quote: ... Clear lysates were filtered through 0.45 μm and immediately applied at 2 mL/min onto a HisTrap HP 5 mL column (GE Healthcare) previously equilibrated in lysis buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was cleared by centrifugation for 45 min at 48.000 xg and loaded onto a HisTrap FF 5-ml column (GE Healthcare). Eluted proteins were incubated with TEV protease at 4 °C overnight and both the His6-tag ...
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bioRxiv - Plant Biology 2020Quote: ... 20 mM imidazole pH 7.5 was added and the solubilized membranes were loaded on a pre-equilibrated 5 ml Ni-NTA column (GE Healthcare) at 3 ml/minute ...
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bioRxiv - Molecular Biology 2021Quote: ... the soluble proteins were collected after a 5 min centrifuge at 17,000 g at 4 ℃ and immobilised on Glutathione-coated sepharose beads (GE Healthcare). For pull down with 35S-radiolabelled preoteins ...
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bioRxiv - Microbiology 2021Quote: ... and were eluted with CA loading buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM BME) using AKTAprime plus (GE Healthcare). CA monomer fractions were concentrated using Amicon Ultra-10K device (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: ... The eluate was then diluted 3x with 20 mM Tris-HCl pH7.5 and passed over a 5 mL Q-sepharose column (GE Healthcare). The Flow through containing the MIF protein was then again loaded on a 5 mL CM sepharose column ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Biochemistry 2020Quote: ... Sample was filtered through a 0.45 μm nitrocellulose filter and loaded onto a 5 ml HisWash1-equilibrated HisTrap HP column (GE Healthcare). 2 ml fractions were collected through a linear gradient over 100ml up to 100% HisElution [50 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were grown at 37°C with 5% CO2 in a humidified atmosphere in DMEM/HIGH glucose (Cat# SH30022.01, GE Healthcare) for SK-N-SH ...
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bioRxiv - Biophysics 2019Quote: ... The cleaved protein was then purified by a 5 ml HisTrap HP column on an AKTA Avant system (GE Healthcare). The flow through was combined and diluted with buffer C ...
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bioRxiv - Biochemistry 2020Quote: ... 250 μM TCEP and 20 mM sodium phosphate at pH 6.5 (ITC buffer) using a 5 mL HiTrap Desalting column (GE Healthcare). Likewise ...
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bioRxiv - Molecular Biology 2021Quote: ... The VHH72-Fc constructs were purified from the clarified culture medium using a 5 ml MabSelect SuRe column (GE Healthcare). After a wash step with McIlvaine buffer pH 7.2 ...
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bioRxiv - Biochemistry 2021Quote: ... Precipitated DNA was pelleted by centrifugation and soluble lysate was loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with 7 column volumes of loading buffer before being eluted with a gradient of imidazole (20–500 mM ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was filtered through a 0.22-μm filter and loaded onto a 5 mL His Trap HP column (GE Healthcare) pre-equilibrated with buffer A (10 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The solution was filtered and the model degrons were loaded on a HiTrap SP HP 5 ml column (GE Healthcare) in 50 mM ammonium acetate pH 4.5 ...
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bioRxiv - Biochemistry 2021Quote: ... then the soluble fraction was filtered through 0.22 μm PVDF membrane before loading onto a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 20 column volumes of 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biophysics 2021Quote: ... Reaction mixtures (18.5 μl) were resolved on 5% Tris-Glycine native gels and imaged on a Typhoon scanner (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... were cultured at 37°C in 5% humidified CO2 in high glucose Dulbecco’s Modified Eagle Medium (DMEM, Sigma or GE Healthcare), supplemented with 4 mM L-glutamine ...
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bioRxiv - Microbiology 2021Quote: ... Purified proteins were concentrated by ultrafiltration and buffer-exchanged into PBS on a 5 ml HiTrap Desalting column (GE Healthcare). The concentration was determined from UV absorbance at 280 nm ...
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bioRxiv - Microbiology 2020Quote: ... Protein sample were injected into a size exclusion column (Superose™ 6 Increase 5/150 GL, GE Healthcare Bio-Sciences) previously equilibrated with corresponding buffer and eluted directly into the SAXS flow-through capillary cell at a flow rate of 0.2 ml/min ...
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bioRxiv - Immunology 2020Quote: ... tissue was minced with scissors into 5 mm pieces and digested three times with 0.25% trypsin (GE Healthcare Life Sciences) for 10 minutes at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... MukBEF was largely found in the flowthrough and was applied to a 5 mL HiTrap Q HP column (GE Healthcare). The column was washed with 2 column volumes (CV ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysates were incubated with 5 ml bed volume Glutathione Sepharose 4B resin (GE Healthcare, cat. No. 17-0756-05) for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... the clarified material was filtered with a 0.22 µm membrane and then applied a HisTrap HP 5 ml (GE Healthcare) and eluted with buffer B (30 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
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bioRxiv - Microbiology 2022Quote: ... We pelleted and lysed bacteria by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 0.5 M imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... was then loaded to a 5 mL HisTrap Ni-NTA column coupled to AKTA Pure protein purification system (GE Healthcare). The His-SUMO-tagged CAP1 protein was eluted from the nickel column with an imidazole gradient using buffer A and buffer B (buffer A + 250 mM imidazole) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a 0.45 μm syringe filter and loaded onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated in binding buffer ...
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bioRxiv - Biophysics 2022Quote: ... pH 7.5) for subsequent anion exchange chromatography on a 5 ml Mono-Q column (GE Healthcare Europe GmbH, Freiburg, Germany). Following an elution gradient with high-salt buffer (20mM TRIS ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Cell Biology 2022Quote: ... Data were collected following fractionation with an in-line size-exclusion chromatography column (Superdex 200 5/150 Increase, GE Healthcare,) pre-equilibrated in TBS pH 8.5 ...
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bioRxiv - Immunology 2022Quote: ... Nb-Fc was diluted to a concentration of 5 μg/ml with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units ...
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bioRxiv - Biochemistry 2022Quote: ... Histones were then further purified by cation exchange on a 5 ml HiTrap SP HP column (GE Healthcare Life Sciences), pre-equilibrated with HGFB150 (full-length histones and tailless H2B) ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 mM dithiothreitol (DTT), and concentrated using Vivaspin 5 kDa (HipBLp, HipSLp) and 10 kDa (HipTLp) cutoff columns (GE Healthcare). Protein concentration was quantified using the Bradford assay according to the manufacturer’s instructions (BioShop) ...
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bioRxiv - Neuroscience 2022Quote: ... The unbound fluorophores were removed by gel filtration on a NAP-5 column (Sephadex-25 medium, GE Healthcare, Munich, Germany). The final antibody concentration and degree of labelling was analyzed on a spectrophotometer (NanoDrop 2000 UV-Vis ...
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bioRxiv - Biochemistry 2022Quote: ... monomeric C9 samples were passed through a Superdex 200 Increase 5/150 GL size-exclusion column (GE Healthcare Life Sciences) in 10 mM HEPESNaOH ...