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Citations for GE Life Sciences :
1701 - 1750 of 3483 citations for 7 8 Dihydro 6H 1 6naphthyridin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... and the soluble His6-M protein was purified on a Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Biochemistry 2023Quote: The filtered cell-free extract was subjected to immobilized metal affinity chromatography using an Äkta pure chromatography system equipped with a 5-mL HisTrap HP column (GE HealthCare) equilibrated with lysis buffer (see above) ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 M NaCl and 30 mM imidazole and captured with Glutathione-Sepharose 4B beads as indicated by the manufacturer (GE Healthcare). The elution fractions were pooled and the buffer was exchanged to phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Capturing of ORF1p-His6 protein was performed using Immobilized-Metal Affinity Chromatography (IMAC) by loading of 7M Urea Extract on 5 mL HisTrap FF FPLC column (GE Healthcare) at 0.5 mL/minute flow rate followed by washing with 10 column volumes (CV ...
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bioRxiv - Biophysics 2022Quote: ... was performed at 20 °C using S75 (IDR constructs) and S200 Increase 5/150 (NTD and ARD constructs) analytical SEC columns (GE Healthcare) equilibrated in the appropriate buffers (see Tables S1 and S2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then washed in PBS-T and incubated with 1 mL of Enhanced ChemiLuminescence (ECL) (Amersham) reagent for 5 minutes to allow protein visualization on an x-ray Amersham Hyperfilm ECL (GE Healthcare). Fiji-Image J Software was used for quantification of the difference in protein expression comparing with GAPDH or HSP70 expression.
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bioRxiv - Biophysics 2023Quote: ... diluted with the same buffer devoid of NaCl to reach a final salt concentration of 40 mM and loaded on a HiSure Q 5 mL column (GE Healthcare). After washing with a 25 mM Na-HEPES ...
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bioRxiv - Microbiology 2023Quote: ... Blots were developed with standard BCIP (5 % (w/v) in DMF) + 250 μL NBT (5 % (w/v) in 70 % DMF) or using the ECL western blotting reagent (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... Lysate was cleared by centrifugation at 20000g for 45 min and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–300 mM in 30 column volumes ...
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bioRxiv - Immunology 2023Quote: ... 6 and 14 we euthanized 5 individuals per group and performed an intra-cardiac terminal blood collection for PBMCs isolation with Ficoll-Paque Plus (GE healthcare). We performed a complete necropsy of all animals and collected samples of the lungs ...
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bioRxiv - Microbiology 2023Quote: ... The gel was dried at 80°C for 1 h prior to exposure to a phosphor-screen for ∼5 h to overnight and imaged using a Typhoon Trio Variable Mode Imager (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... The fractions collected at the elution peak were pooled together and loaded into a MonoS 5/50 GL column (GE Healthcare) previously equilibrated with IEX Equilibration buffer (50 mM HEPES pH7.8 ...
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bioRxiv - Biochemistry 2023Quote: ... DAP5 was first purified from bacterial cell lysate using a 5 mL His-Trap HP (Ni-NTA) column from GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 or Amicon 15 centrifugal concentrators (10 kDa, 5 kDa, or 3 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were run at 4 °C overnight as described above and scanned with GE Amersham Typhoon-5 fluorescence gel and blot scanner (GE Healthcare) with red laser.
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions from ion-exchange chromatography were filtered through a 5 µm syringe filter and loaded onto a 22 mL Source 15ISO hydrophobic interaction column (GE Healthcare) equilibrated in buffer C (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... rPF4 was purified from soluble fractions of cell lysates by affinity chromatography using a 5-ml HiTrap heparin-agarose column (GE Healthcare). Endotoxin-free rPF4 was prepared as previously described 3 ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... The soluble fraction was filtered through a 0.45 μm sterile syringe filter and loaded onto a pre-equilibrated 5 mL HiTrap IMAC HP column (GE Healthcare Biosciences). The column was washed with 4 column volumes of lysis buffer followed by 4 column volumes of wash buffer 1 [50 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... Imidazole and NaCl present in the Ni-NTA running buffer were eliminated by using a HiTrap Desalting 5 mL (GE Healthcare) column.
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were probed with ECL Plex Cy3- or Cy5-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Cytiva) and scanned on an Amersham Typhoon 5 Phosphorimager (GE Healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation with 20,000 x g followed by filtration with a 5 μM filter and loaded on a HisTrap HP column pre-equilibrated with lysis buffer (GE Healthcare). The column was washed first with lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Labelling reactions proceeded for 40 mins at 4°C and the excess dye was removed using NAP-5 desalting columns (17-0853-01, GE Healthcare). Nanobodies to be used for confocal microscopy were labelled with Alexa488 (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... and the supernatant was filtered and loaded onto a 5 mL HisTrap HP column (Cytiva) using an AKTA Start (GE Healthcare). The column was washed in buffer containing 50 mM Tris-HCl (pH 8) ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was treated with 100 mM phosphate buffer pH 7.5 and 50 mM EDTA before purification by gel filtration at room temperature on a Superose-6 Increase column (GE Healthcare) equilibrated with buffer containing 25 mM TRIS pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Rab35BD and Rac1BD-Rab35BD) (40 µL; 10-25 mg/ml) were loaded onto a Superdex-200 increase 5/150 column (GE Healthcare) or BioSec5 300 (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation with 20,000 x g at followed by filtration with a 5 μM filter before loading on a HisTrap HP column (GE Healthcare). The column was washed first with lysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then diluted with dilution buffer (50 mM HEPES (pH 7.2) and 5% glycerol) to reduce the salt concentration and loaded onto a Mono S 10/100 GL column (GE Healthcare). The column was washed with buffer C (50 mM HEPES (pH 7.2) ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) pre-equilibrated with the HiTrap binding buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was loaded onto a pre-washed/pre-equilibrated CM Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL) on an Äkta explorer FPLC (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The clear top phase obtained above a white band was collected and separated over a 5 ml HiTrap Q FF column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... A 2-fold molar excess of LF was mixed with 925 pmoles of hexon and incubated on ice for 5 min followed by SEC on Superose 6 increase 10/300 (GE Healthcare) equilibrated with buffer D ...
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bioRxiv - Cell Biology 2024Quote: ... The eluate was concentrated to a volume of 5 mL and loaded onto a size-exclusion-chromatography column (Superdex 16/60, GE Healthcare); HR3 LBD was eluted in a buffer of 150 mM NaCl ...
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Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... and equal amounts of protein were separated on 5–20% SDS‒PAGE gels (Wako) and transferred to Hybond-P membranes (GE Healthcare). The following primary antibodies and dilutions were utilized ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The urea concentration was set to 6 M and the sample was applied to a 5 mL HiTrap QFF anion exchange column (GE healthcare) and was eluted using a 30 CV long gradient from low salt buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to ∼2 µM for cryo-EM sample preparation.
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bioRxiv - Plant Biology 2024Quote: ... the untagged protein was separated from cleaved tags and the 6xHis-tagged 3C protease by affinity chromatography using 5 ml HisTrap HP NTA column (GE Healthcare). If proteins were also tagged with MBP ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) preequilibrated with the HiTrap binding buffer ...
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bioRxiv - Neuroscience 2024Quote: Molecular masses of purified gephyrin and variants were detected by analytical size exclusion chromatography (SEC) using a Superose 6 increase 5/10 (GE Healthcare). Typically ...
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bioRxiv - Neuroscience 2024Quote: ... RNA (5 μg) was used to synthesize the first-strand complementary DNA (cDNA) using the First-Strand cDNA synthesis kit (GE Healthcare) with a pd(N)6 primer following the manufacturer’s indications ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were centrifuged to remove cell debris at 20,000 x g for 60 min prior to loading of a 5 mL HiTrap SP-FF column on an ÄKTA purifier FPLC (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were filtered and then pelleted at 300 g for 5 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 900 g for 20 minutes with acceleration set to 4 and deceleration set to 1 in order to remove cellular debris ...
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bioRxiv - Microbiology 2024Quote: ... fully complementary to the fluorescent strand to avoid it re-hybridizing to the quencher strand) and 5 mM ATP (GE Healthcare) in a 100 µL reaction solution containing 10 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... participants were asked to lay in the supine position for a minimum 5 minutes prior to full-body dual-energy x-ray absorptiometry (DXA) scan (Lunar Prodigy, GE Healthcare). Lean/soft tissue mass (also commonly termed as lean body mass ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes are subjected to five more washes in TBS-T before being incubated in chemifluorescence substrate for a maximum of 5 minutes and scanned on either a Storm 860 Scanner (Amersham Biosciences) or Typhoon FLA 9000 Scanner (GE Healthcare) or being visualized in a ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Cancer Biology 2024Quote: ... 60 μl of this mixture was added in the HUVEC side channel of the chip and max intensity projections were taken every 5 minutes for 30 minutes using the IN Cell Analyzer 6000 (GE Healthcare) microscope.
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bioRxiv - Biochemistry 2024Quote: ... Gels were fixed with 5% cold trichloroacetic acid and then dried onto chromatography paper (Whatman) and autoradiographed with Amerhsam Hyperfilm-MP (GE Healthcare). Gel images were scanned using a Typhoon phosphorimager (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was then filtered through 0.2 µm PES filter membrane and loaded onto a HisTrap HP column (5 ml, 1.6 x 2.5 cm, Ni Sepharose High-performance column, GE Healthcare, now Cytiva) pre-equilibrated with buffer A at 1 mL/min ...
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bioRxiv - Biochemistry 2024Quote: ... The splicing product was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare) which is pre-equilibrated with complex buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 2µM for cryo-EM sample preparation.