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Citations for GE Life Sciences :
1151 - 1200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... After centrifugation the clarified supernatant was applied to a GSTrap FF sepharose column (GE Healthcare) and washed with high-salt (1 M NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... uncleaved protein as well as cleaved GST were removed by applying everything onto 5 ml SP column (GE Healthcare) equilibrated with 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was applied to a HisTrap-FF sepharose column (GE Healthcare) and washed with low-salt buffer (500 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... consisting of a Protran nitrocellulose membrane (Whatman) followed by a Hybond N+ nylon membrane (GE Healthcare Bioscience). Subsequently ...
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bioRxiv - Biochemistry 2024Quote: ... The splicing product was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare) which is pre-equilibrated with complex buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... then IntC-UbcH5c-CAET-Ub was refolding and purified by size-exclusion chromatography (Superdex 75 10/300 GL column, GE Healthcare). The refolded IntC-UbcH5c-CAET-Ub-SH was mixed with IntN-RNF168 to initiate the protein trans-splicing reaction and finally obtained the RNF168-UbcH5c-CAET-Ub.
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bioRxiv - Biochemistry 2024Quote: ... and size-exclusion chromatography (Superdex 75 10/300 GL column, GE Healthcare). IntC–UbcH5c was purified similarly ...
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bioRxiv - Biochemistry 2024Quote: ... The splicing product was concentrated to a volume of ∼500 µL and loaded onto a Superose 75 10/300 GL size-exclusion column (GE Healthcare) which is pre-equilibrated with complex buffer (20mM HEPES,150mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... and size-exclusion chromatography (HiLoad 16/600 Superdex 75 prep grade column, GE Healthcare). SET8 was purified in the same way as RNF168RING ...
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bioRxiv - Biochemistry 2024Quote: ... and size-exclusion chromatography (Superdex 200 10/300 GL column, GE Healthcare). RNF168RING and RNF168RING–IntN were purified by Ni affinity chromatography ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 2µM for cryo-EM sample preparation.
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bioRxiv - Biochemistry 2024Quote: ... it was purified by size-exclusion chromatography (Superdex 75 10/300 GL column, GE Healthcare). The peak fraction was collected and flash-frozen in liquid nitrogen
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bioRxiv - Biochemistry 2024Quote: ... followed by anion exchange chromatography (AEC, HiPrep Q HP 16/10, GE Healthcare) as previously described [14] ...
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bioRxiv - Biochemistry 2024Quote: ... The refolded WT- and E111V-GCAP1 variants were purified by size exclusion chromatography (SEC, HiPrep 26/60 Sephacryl S-200 HR, GE Healthcare) followed by anion exchange chromatography (AEC ...
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bioRxiv - Biochemistry 2024Quote: ... Different protein dilutions ranging from 0.8 µM to 80 µM were prepared and injected (200 µL) into a Superose 12 10/300 column (GE Healthcare) previously equilibrated with a 20 mM Tris-HCl pH 7.5 buffer containing 150 mM NaCl and 0.5 mM EGTA + 1 mM Mg2+ or 1 mM Mg2+ + 0.5 mM Ca2+ ...
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bioRxiv - Cancer Biology 2024Quote: ... Detection and quantification were performed using HRP-coupled secondary antibodies and and an Image Quant LAS4000 chemiluminescence-based detection system (enhanced chemiluminescence) (GE Healthcare Life Science).
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal bovine serum (HyClone, GE Healthcare Life Sciences), 2 mM L-glutamine ...
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bioRxiv - Cancer Biology 2024Quote: ... All other cell lines were grown in Dulbecco’s Modified Eagle’s Medium (GE Healthcare Life Sciences, Logan, UT) with 10% fetal bovine serum (10% FBS–DMEM) ...
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bioRxiv - Cancer Biology 2024Quote: ... Twenty micrograms of total protein were loaded either on a 12% polyacrylamide-SDS gel and transferred to a 0.2 µm nitrocellulose membrane (No10600001, GE Healthcare; Chicago, IL, USA). Membranes were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... the corrected sensograms were analysed by employing BIA-T200 evaluation software version 2.1 (GE Healthcare). The relation between protein concentration and the Response unit (RU ...
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bioRxiv - Cell Biology 2024Quote: ... Images were collected as a Z-stack and deconvolved using SoftWorx (GE HealthCare), then displayed as a maximum intensity projection ...
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bioRxiv - Cancer Biology 2024Quote: ... DRG neurons were further purified using a Percoll (GE Healthcare, 17-0891-01) gradient (12.5% and 28%) ...
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bioRxiv - Cancer Biology 2024Quote: ... for 3-5 minutes and visualized using Amersham Imager 600 (GE Healthcare).
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bioRxiv - Cancer Biology 2024Quote: ... Bound antibodies were detected by ECL (GE Healthcare). DNA loading was assessed by re-probing the membrane with radiolabeled mouse genomic DNA with ethidium bromide (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were obtained from GE Healthcare (Chicago, IL, USA). Bound antibodies were detected by ECL (GE Healthcare) ...
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bioRxiv - Cancer Biology 2024Quote: ... Amersham ECL Prime Western blotting detection reagent (GE Healthcare) was used to detect immunoreactive bands ...
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bioRxiv - Cancer Biology 2024Quote: ... transferred onto a nitrocellulose membrane (Hybond-ECL, GE Healthcare Life Science) and western blotted ...
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bioRxiv - Cancer Biology 2024Quote: ... Signal was detected using enhanced chemiluminescence (ECL) (GE Healthcare Life Sciences, USA) and ChemiDoc imaging system (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... for 1 hour at room temperature and visualized using the ECL detection system (GE Healthcare).
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bioRxiv - Cell Biology 2024Quote: ... Labelled protein bands were detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, USA) and ChemiDoc MP Imaging System (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... with Amersham HMW native marker kit (17044501; GE Healthcare) as a molecular weight marker ...
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bioRxiv - Cell Biology 2024Quote: Col1a1-H2B-GFP;Rosa26-M2-rtTA;Slc23a2FL/FL;Mx1-Cre mice and Col1a1-H2B-GFP;Rosa26-M2-rtTA;Slc23a2FL/FL littermate controls were given 3 intraperitoneal injections of 40µg of poly I:C (GE Healthcare) dissolved in PBS over 5 days to induce Cre expression ...
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bioRxiv - Cell Biology 2024Quote: ... Mx1-Cre mice were given 3 intraperitoneal injections over 5 days of 40µg of polyinosinic-polycytidilic acid (poly I:C) (GE Healthcare) dissolved in PBS ...
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bioRxiv - Cell Biology 2024Quote: Super-resolution Structural Illumination Microscopy (SIM) imaging was performed on a Deltavision OMX V4 SIM microscope (GE Healthcare) equipped with an Olympus 60×/1.42 ...
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bioRxiv - Cell Biology 2024Quote: ... Live imaging was carried out in a chamber at 37°C using a Deltavision Core (Applied Precision™) microscope equipped with a CoolSnap HQ2 (Photometrics ...
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bioRxiv - Cell Biology 2024Quote: ... controlled by DeltaVision softWoRx (GE Healthcare) using a 60x 1.42 NA UPlanS Apochromat objective lens (Olympus) ...
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bioRxiv - Cell Biology 2024Quote: ... Water-swollen thiopropyl sepharose 6B beads (GE Healthcare Life Sciences, Mississauga, ON) were added and samples were incubated 2 hrs at RT on rotating wheel ...
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bioRxiv - Cell Biology 2024Quote: ... An extra purification step of Size exclusion chromatography was carried out using an S75 16/600 column (GE healthcare) equilibrated against a PBS buffer pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were detected using a luminescence image analyzer (LAS-4000 mini; GE Healthcare) or a luminescence image analyzer (FUSION Solo S ...
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bioRxiv - Biophysics 2024Quote: ... Protein was further purified and buffer exchanged by size exclusion chromatography using HiLoad 16/600 Superdex-G-200 (GE Healthcare) gel filtration column to storage buffer (50mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... His6-GB1-tag was subsequently cleaved by TEV protease (3 mM) and separated from bZIP on a cation exchange column (HiTrap SP; GE Healthcare) using a 0–2 M gradient of sodium chloride ...
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bioRxiv - Biophysics 2024Quote: ... The soluble fraction of gp24 was incubated with glutathione sorbent slurry (Glutathione Sepharose 4B; GE Healthcare Life Sciences), washed with phosphate buffer ...
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bioRxiv - Biophysics 2024Quote: ... the protein was purified by size exclusion chromatography using a Sephadex 200 16/60 (GE Healthcare) column with the lysis buffer.
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bioRxiv - Biophysics 2024Quote: ... Immunoblots were revealed with ECL Prime Western Blotting Detection Reagent (GE Healthcare) in High Performance Chemiluminescence Film (GE Healthcare).
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bioRxiv - Biophysics 2024Quote: ... Final purification was achieved using SEC (Superdex 75 16/60, GE Healthcare) equilibrated in Buffer D (20 mM Hepes ...
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bioRxiv - Biophysics 2024Quote: ... in High Performance Chemiluminescence Film (GE Healthcare).
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bioRxiv - Biophysics 2024Quote: ... The flow-through fraction was mixed with CM Sepharose FF resin (GE HealthCare Lifesciences) on a rotator at 4 °C over night and passed through an open column to remove resin ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant of the cell lysate was loaded onto a HisTrap column (5 mL-GE Healthcare) and the bound protein was eluted with an imidazole gradient (0-1 M) ...
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bioRxiv - Biophysics 2024Quote: ... Abl83-534 was then purified using an ion exchange Q-sepharose HP (GE Healthcare) column equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... The eluate was concentrated and applied onto a Superose 6 Increase 10/300 column (GE Healthcare) for size exclusion chromatography (SEC ...