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Citations for GE Life Sciences :
1001 - 1050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Eluate fractions were pooled and loaded onto a size exclusion column (HiLoad Superdex 26/600 200 prep grade, GE Healthcare) pre-equilibrated with Tris buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing desired protein were pooled and concentrated using a 100 kDa centrifugal concentrator (Amicon UFC810024) and loaded on gel filtration chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) using the following buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was applied onto a carboxymethyl sepharose fast flow column (GE Healthcare) and eluted with a sodium chloride gradient that contained 50 mM MES pH 6.5.
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bioRxiv - Biochemistry 2024Quote: ... TBC-DEG-αβ-tubulin assemblies were purified by size exclusion chromatography (SEC) using a Superdex 200 10/300 column (GE Healthcare, USA) in binding buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... and then loaded on a HiLoad 16/600 Superdex-200 gel filtration column (GE Healthcare, USA). TBC-DEG was then concentrated ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded onto a HisTrap FF column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were spotted onto Amersham Protran nitrocellulose membrane (GE Healthcare) filter sheets ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Optitran BA-S 85, pore size 0.45 µm, GE Healthcare) using a semi-dry blotting chamber at a constant current of 140 mA/gel for 60 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... ADALP1 was purified by size exclusion chromatography using a S75 16/60 GE Column (GE Healthcare) equilibrated in buffer containing 10 mM Tris pH = 8.0 ...
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bioRxiv - Biochemistry 2024Quote: All live cell microscopy was performed on a DeltaVision Elite Deconvolution microscope (Applied Precision, Issaquah, WA, USA). Imaging pads were stained with 8 µL of dye mix (25 µg/mL DAPI ...
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bioRxiv - Biochemistry 2024Quote: ... membranes were washed and incubated with secondary anti-mouse peroxidase-conjugated antibodies (GE Healthcare NA931V) for 1h ...
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bioRxiv - Biochemistry 2024Quote: ... using a sCMOS pro.edge camera (GE Healthcare, Applied Precision). Image analysis was performed using FIJI (Schindelin et al. ...
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bioRxiv - Biochemistry 2024Quote: ... supernatant was loaded onto a pre-packed 5 ml HisTrap column (GE Healthcare), equilibrated according to the supplier’s protocols with wash buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... using a sCMOS pro.edge camera (GE Healthcare, Applied Precision). Image analysis was performed using FIJI (Schindelin et al. ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was then diluted to a final salt concentration of low salt buffer conditions (100 mM KCl, 50 mM HEPES, 1 mM MgCl2) and bound to HiTrap SP FF (GE Healthcare, USA) anion exchange column ...
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bioRxiv - Biochemistry 2024Quote: ... TBC-DEG-αβ-tubulin assemblies were purified by size exclusion chromatography (SEC) using a Superdex 200 10/300 column (GE healthcare) in binding buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... and images were captured on ImageQuant LAS 4000 (GE Healthcare) device ...
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bioRxiv - Biochemistry 2024Quote: ... The membranes were revealed using the ECL kit (GE Healthcare RPN2232), and images were captured on ImageQuant LAS 4000 (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... gels were imaged using Typhoon-9400 scanner (GE Healthcare) with a resolution of 100 μm using appropriate emission and excitation wavelengths ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was quenched with 1 μl 14.3 M βME followed by final buffer exchange using size exclusion chromatography (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... The second dimension run was performed in an EttanDalt Six Electrophoresis Unit (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... and further purified by size exclusion chromatography using a Superdex 75 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: Relative protein spot quantification across experimental conditions was performed using DeCyder v7.0 software and multivariate statistical module EDA v7.0 (Extended Data Analysis, GE Healthcare), as follows ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM βME was added to reduce cysteine residues followed by buffer exchange using a HiTrap 26/10 Desalting column (GE Healthcare) in 25 mM HEPES (pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with Glutathione Sepharose 4B (GE Healthcare). GST was cleaved from protein by TEV protease treatment for 16 hours at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The purified receptor was subsequently concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and run on size-exclusion chromatography on a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated with 30 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... samples were loaded onto a Superose 6 10/300 gel filtration column under the control of an ÄKTA Pure instrument (GE Healthcare Life Sciences). Proteins were eluted using 50 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... Both tagged and cleaved BtuG samples were then loaded on a NAP-10 desalting column (kinetic assays, GE healthcare) for buffer exchange to 20 mM Tris/HCl pH 7.5 and 100 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: A second purification step on Ni-NTA beads was performed to remove any uncleaved protein before to achieve a SEC purification onto a Superdex 75 16/600 GE column (GE Healthcare) in a final buffer containing 10 mM Tris pH = 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... and loaded onto a HisTrap chelating column (GE Healthcare) running on an AKTA™ FPLC workstation ...
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bioRxiv - Biophysics 2024Quote: ... and 40 mM DTT) after further purification by gel filtration with a Superdex 200 increase GL 10/300 (GE Healthcare, USA). Purified proteins were checked by SDS-PAGE.
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bioRxiv - Biophysics 2024Quote: To analyze the VWFA1-GPIbα interaction by surface plasmon resonance (SPR; Biacore 3000; GE Healthcare), human recombinant GPIbα headpiece followed by 133 residues of the SV40 large T antigen with Cys residues mediating dimerization was generated ...
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bioRxiv - Biophysics 2024Quote: ... we loaded the protein solution onto a HiTrap Q FF column (GE Healthcare) and eluted it with a linear gradient of 0–1 M NaCl in 20 mM sodium phosphate at pH 6.0 ...
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bioRxiv - Biochemistry 2024Quote: ... the soluble fraction was incubated in batch for 2 h with glutathione Sepharose 4B (GE Healthcare), previously equilibrated in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting ABC holotoxins were then separated from excess TcB-TcC by size exclusion chromatography on a Superose 6 increase 5/150 column (GE Life Sciences) equilibrated in labelling buffer ...
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bioRxiv - Biochemistry 2024Quote: ... ImageQuant TL (GE Healthcare) was used for image quantification ...
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bioRxiv - Bioengineering 2024Quote: ... which was transferred to the Hybond-N membranes (GE Healthcare, Chicago, IL) using capillary flow transfer method ...
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bioRxiv - Bioengineering 2024Quote: ... 2 μg DNA loaded per lane was separated by electrophoresis on 1% agarose gels and transferred to Hybond-N membranes (GE Healthcare, Chicago, IL) using capillary flow transfer method ...
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bioRxiv - Bioengineering 2024Quote: ... Radioactive probes were prepared by random-primed 32P labeling (GE Healthcare, Chicago, IL) of gel-purified DNA fragments of the following genes ...
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bioRxiv - Bioengineering 2024Quote: ... Hybridazation results were imaged with a Typhoon RGB scanner (GE Life Sciences, Chicago, IL) and quantified with ImageJ software (version 2.0.0-rc-38/1.50b).
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bioRxiv - Biophysics 2024Quote: ... the remaining 10 ml of concentrated 6-His-AdhE in buffer A were loaded onto a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biophysics 2024Quote: ... followed by further purification with a MonoQ 10/100 GL column (GE Healthcare) using a 0.75–1 M salt gradient in the same buffer ...
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bioRxiv - Biophysics 2024Quote: ... were used with flow rates at 6 ml/min and loaded either by hand or by using an AKTA Purifier (GE Healthcare) following manufacturers guidelines ...
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bioRxiv - Biophysics 2024Quote: ... and injected onto a HiLoad 16/600 Superdex 75 pg column (GE Healthcare) equilibrated with storage buffer (25 mM Tris-HCl buffer pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... We then further purified the supernatant by size exclusion chromatography on a HiLoad 26/600 Superdex 75 pg column (GE Healthcare) pre-equilibrated with the same buffer ...
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bioRxiv - Biophysics 2024Quote: ... and lysed using an EmulsiFlex C5 homogenizer (Avestin) at 10-15,000 pounds per square inch followed by passage over a HisTrap column (GE Healthcare) and elution with 300 mM imidazole in lysis buffer ...
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bioRxiv - Biophysics 2024Quote: ... 5 mL HisTrap excel columns (GE Healthcare, column pressure limit = 0.5 PSI) were used with flow rates at 6 ml/min and loaded either by hand or by using an AKTA Purifier (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... Samples were run for 2 h at 90 V and then scanned using a Typhoon FLA 9500 laser scanner (GE Healthcare) at a pixel size of 50 µm ...
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bioRxiv - Biophysics 2024Quote: ... pH 7.4 by using a HiPrep Desalting column (GE Healthcare) before snap freezing and storing at −80°C.