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Citations for GE Life Sciences :
1251 - 1300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Proteins were detected on a LAS-4000 (GE Healthcare) in SYBR-green fluorescence detection mode and Image Quant TL software.
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bioRxiv - Cell Biology 2024Quote: ... 300 µg of total protein extracts were purified using Streptavidin Sepharose High-Performance beads (GE Healthcare Bio-Sciences AB). For western blot analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... HRP-conjugated mouse secondary antibody (GE Healthcare) was used at 1:10,000 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... and goat-anti-mouse (GE Healthcare) secondary antibodies were used at concentrations of 1:10,000.
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bioRxiv - Cancer Biology 2024Quote: ... 15 mL of Ficoll-Paque™ Plus (#17-1440- 03, GE Healthcare,) was dispensed to a 50 mL SepMate™ (#85450 ...
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bioRxiv - Cancer Biology 2024Quote: ... CM5 Sensor chip for SPR (cat no: 50-105-5511) was purchased from GE Healthcare Bio-Sciences (Cytiva life sciences-USA) ...
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bioRxiv - Cancer Biology 2024Quote: ... Band intensities were quantified using an ImageQuant LAS 500 system (GE Healthcare, IL, USA). The same experiments were repeated for spheroids and organoids treated with Bevacizumab or transfected with miR200c ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was measured by Bradford assay and 20 µg of denatured protein (in Laemmli buffer at 95°C for 5 min) was loaded in a 7.5% polyacrylamide gel and blotted onto a nitrocellulose membrane (GE Healthcare, Buckinghamshire, UK). The membranes were blocked in 5% milk in PBS with 0.01% Tween (PBST ...
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bioRxiv - Neuroscience 2024Quote: ... HRP activity was detected by enhanced chemiluminescence using the ImageQuant™ LAS 500 detector (GE Healthcare). Band intensity was evaluated with the Image J software using β-actin or α--tubulin band density as loading control ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were resolved onto an 8% SDS-PAGE and transferred to Protran Premium NC membranes (GE Healthcare). The membranes were blocked with milk (5% [w/v] dried milk in 1X PBST (137mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... HRP-conjugated anti-rabbit IgG (1:2500, NA934V, GE Healthcare, Fairfield, CT) and HRP-conjugated anti-mouse IgG (1:2500 ...
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bioRxiv - Biophysics 2024Quote: ... The last step was a gel filtration on a Superdex 75 column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... The sample was applied onto a Heparin column (GE Healthcare) which had previously been equilibrated with buffer E ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was cleared by centrifugation and loaded on a Ni-NTA agarose column (HisTrap HP; GE Healthcare). After rigorous washing with 100 mM Tris-HCl pH 8.5 ...
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bioRxiv - Biophysics 2024Quote: ... the protein mixture was loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer B ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was filtered in 0.45 µm filter and loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... 100 mM NaCl for all proteins with PD10 columns (GE Healthcare) and protein concentrations were determined by the Bradford assay (Biorad) ...
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bioRxiv - Biophysics 2024Quote: The affinity of RCC1 for nucleosome was measured on a Biacore 8K biosensor (GE Healthcare) by surface plasmon resonance (SPR) ...
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bioRxiv - Biophysics 2024Quote: ... to remove metal ions and polysulfane sulfur before proteins were subjected to anaerobic size exclusion chromatography (SEC) (HiLoad 16/600 Superdex; GE Healthcare) on an Äkta Purifier system (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... on an Äkta Purifier system (GE Healthcare) using SEC buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... ISCU2 and FXN were subjected to Ni-NTA affinity chromatography (His-Trap 5ml FF crude; GE Healthcare). The column was washed with 10 CV IMAC wash buffer ...
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bioRxiv - Bioengineering 2024Quote: - a pre-operative CT scan taken at late diastole with a Discovery HD750 scanner (GE Healthcare) using the following configuration ...
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bioRxiv - Biophysics 2024Quote: ... Relevant fractions were combined and run over size-exclusion (HiLoad S200 16/60 prep grade, GE Healthcare) with sample buffer (20 mM Tris ...
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bioRxiv - Biophysics 2024Quote: ... The protein was further purified using a 5 mL HiTrap Heparin HP column (GE Healthcare), and then eluted with a linear gradient of 0.1-0.5 M NaCl ...
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bioRxiv - Biophysics 2024Quote: ... the protein was passed through a 5 mL HisTrap HP column (GE Healthcare), and the cleaved protein was collected in the flow through ...
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bioRxiv - Biophysics 2024Quote: ... Eluted product was loaded on a HiLoad 16/60 Superdex 200 prep grade column (GE Healthcare) and run with a sample buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... The N-terminal GFP-His10 tag was cleaved overnight with 3C protease before gel filtration chromatography by using a Sepharose 6 Increase 10/300 GL column (GE Healthcare). For the purification of apo proteins ...
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bioRxiv - Biophysics 2024Quote: ... Eluted proteins were further purified by size-exclusion chromatography (HiLoad 26/600 superdex 200 pg, GE Healthcare Life Sciences) in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biophysics 2024Quote: ... The flow-through was further purified by size-exclusion chromatography (Superdex 200 Increase 10/300 GL size exclusion column GE Healthcare Life Sciences) in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Bioengineering 2024Quote: ... in combination with an ultrasound contrast agent (Optison – GE HealthCare, Chicago, IL), as illustrated in Supplementary Figure 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood mononuclear cells were obtained by density centrifugation using Ficoll Paque PLUS (GE Healthcare). Migration of PBMCs from 3 donors was assessed using 24-well transwell chambers ...
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bioRxiv - Cell Biology 2024Quote: ... Images were deconvolved using SoftWorx 5.0 (Applied Precision/GEHealthcare). Alternatively ...
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bioRxiv - Cell Biology 2024Quote: Fluorescent images were collected as either 0.25-μm or 0.5-μm Z-stacks on a Personal Deltavision microscope system (Applied Precision/GEHealthcare, Issaquah, WA) using a 60x 1.42 NA lens (Olympus ...
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bioRxiv - Cell Biology 2024Quote: ... Images were reconstructed using softWoRx software from GE Healthcare.
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bioRxiv - Cell Biology 2024Quote: ... EGFP-Kif9 motility was observed in TIRF on the growing red plus-end extensions of MTs using a Personal Deltavision microscope (Applied Precision) with 4-laser TIRF capabilities and an Olympus 60X 1.49 NA TIRF objective at 37 °C.
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bioRxiv - Biochemistry 2024Quote: ... or biotinylated CDO1 (bio-CDO1) was immobilized on a streptavidin chip using a Biacore T200 or a Biacore 8K (GE Healthcare). To determine the kinetics-affinity of the small molecules for proteins ...
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bioRxiv - Cell Biology 2024Quote: ... and 10 mM Tris (pH 8.2) using an AKTA FPLC apparatus (GE Healthcare). After using SDS-PAGE to confirm the purity of eluted fractions ...
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bioRxiv - Cell Biology 2024Quote: ... The eluate was concentrated to a volume of 5 mL and loaded onto a size-exclusion-chromatography column (Superdex 16/60, GE Healthcare); HR3 LBD was eluted in a buffer of 150 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... pH 8.5) and loaded onto a Source 30Q anion-exchange column (GE Healthcare). Using a linear NaCl gradient (30-mM to 2-M NaCl in Qο) ...
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bioRxiv - Cell Biology 2024Quote: ... the protein was eluted using an AKTA FPLC (GE healthcare) and again checked for purity using SDS-PAGE.
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bioRxiv - Cell Biology 2024Quote: ... 10% fetal bovine serum (FBS) (GE Healthcare, Chicago, IL, USA), and a mixture of 100 units/ml penicillin and 100 μg/ml of streptomycin (P/S ...
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bioRxiv - Biochemistry 2024Quote: ... Protein samples were resolved in 12% SDS-PAGE gels to detect TAMRA fluorescence using Typhoon FLA 9500 (GE Healthcare) and images were processed and formatted using ImageJ.
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bioRxiv - Biochemistry 2024Quote: The binding affinity of b96.11 to apo- and holo- GAD65 was measured using surface plasmon resonance (BIAcore T-100, GE Healthcare). Purified monoclonal b96.11 IgG antibody was captured on a CM5 sensor chip with immobilised Protein G ...
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bioRxiv - Biochemistry 2024Quote: apoGAD65 and b96.11 Fab were purified as described and complex formation performed by mixing freshly purified GAD65 with 2X molar excess of b96.11 followed by incubation on ice for 15 min before subjecting the complex to analytical size exclusion chromatography using a Superdex 200 Increase 10/300 GL (GE Healthcare). The sample was used as is for negative stain EM ...
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bioRxiv - Biophysics 2024Quote: ... Germany) and gel filtration fast protein liquid chromatography (FPLC) was performed on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences). The sample was dialyzed against phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Cellular lysates were cleared by centrifugation (14,000 rpm, 20 min) and then incubated (30 min) with Protein G-Sepharose beads (GE Healthcare, Mississauga, ON, Canada) followed by subsequent centrifugation (14,000 rpm ...
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bioRxiv - Biochemistry 2024Quote: ... and then examined with an InCell 6000 Imaging System (GE Healthcare). For visualization ...
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bioRxiv - Biochemistry 2024Quote: ... Quantification was performed by densitometry with ImageQuant TL software (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... Membranes were incubated for 1 h with horseradish peroxidase (HRP)-conjugated secondary antibody (dilution 1:10,000) and developed with enhanced chemiluminescence (ECL) reagent (GE Healthcare). All western blots were visualized with a LAS4000 Imaging Station (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... All western blots were visualized with a LAS4000 Imaging Station (GE Healthcare), ensuring that the representative signal occurred in the linear range ...