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Citations for GE Life Sciences :
1201 - 1250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... The protein sample was passed through His Trap™ FF crude column (GE Healthcare, Piscataway, NJ). We expected the undigested protein to remain bound to the column ...
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bioRxiv - Biophysics 2024Quote: ... A total of 250 µL of each protein was injected and run on a Superdex 200 Increase (S200) 10/300 column (GE Healthcare) at a flow rate of 0.40 mL/min in SEC-MALS buffer (10 mM Tris pH 8.3 ...
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bioRxiv - Biophysics 2024Quote: ... Free Alexa Fluor 555 was then separated from RepA-Titinx-Alexa Fluor 555 using a HiPrep 26/10 desalting column (GE Healthcare, Piscataway, NJ). The labeled protein sample was dialyzed into storage buffer (40 mM Tris ...
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bioRxiv - Biophysics 2024Quote: ... The truncated Abl mutants did not bind to either ion exchange Q- or S-sepharose HP (GE Healthcare) columns in ion exchange buffer ...
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bioRxiv - Biophysics 2024Quote: ... was fed into the X-ray beam after passing through a Superdex 200 (S200) Increase 10/300 GL column (GE Healthcare) in SAXS buffer (50mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... the protein sample was passed through a Hi-Trap Q-Sepharose FF crude (GE Healthcare, Piscataway, NJ) column ...
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bioRxiv - Biophysics 2024Quote: ... The sample was again filtered to 0.2 µm and loaded onto a HiLoad 16/600 Superdex 75 column (GE Healthcare) and eluted as a monomer ...
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bioRxiv - Biophysics 2024Quote: ... connected to an AKTA Pure FPLC (GE Healthcare). Under a flow rate of 0.5 mL/min ...
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bioRxiv - Biophysics 2024Quote: ... loaded onto a His-Trap column (GE Healthcare), and eluted by applying a linear imidazole gradient from 20 mM to 200 mM over 30 column volumes ...
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bioRxiv - Biophysics 2024Quote: ... the protein was concentrated to approximately 500 µL using centrifugal spin filters (Milipore) and injected over a Superdex 200 (S200) Increase 10/300 column (GE Healthcare) connected to an AKTA Pure FPLC (GE Healthcare) ...
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bioRxiv - Biophysics 2024Quote: ... the complex was purified by a Superdex 200 10/300 column (GE Healthcare) equilibrated with buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were loaded onto a Superdex 200 Increase 100/300 GL column (GE Healthcare) and the correct fractions were pooled ...
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bioRxiv - Biophysics 2024Quote: ... The filtrate was passed through StrepTrap HP 5 mL column (GE Healthcare) using 100 mM Tris-HCl pH 8.0 and 150 mM NaCl (Buffer A) ...
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bioRxiv - Biophysics 2024Quote: ... The fractions containing the protein were collected and concentrated using 100 kDa cutoff concentrator and loaded onto Superdex 200 10/300 GL column (GE Healthcare) using 20 mM Tris-HCl pH 7.5 and 150 mM NaCl ...
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bioRxiv - Biophysics 2024Quote: ... The reaction was loaded onto a HisTrap HP nickel column (GE Healthcare) pre-equilibrated with the binding buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2024Quote: ... followed by a Superdex 200 column (GE healthcare) in the same buffer.
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bioRxiv - Biophysics 2024Quote: ... A calibration curve was produced for eluting species using known protein standards (GE Healthcare Life Sciences). The dimerisation-impaired D123P construct of Ire1-LD was used to assign the apparent molecular weight of eluted monomeric protein ...
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bioRxiv - Biophysics 2024Quote: All size exclusion chromatography was carried out using HMK buffer with 5 mM DTT added unless stated otherwise using a Superdex 200 10/300 column (GE healthcare Life Sciences). For each experiment ...
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bioRxiv - Biophysics 2024Quote: ... Supernatants were loaded onto a HisTrap HP nickel column (GE Healthcare) pre-equilibrated with the binding buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2024Quote: ... Fractions containing >95% pure RyR1 were pooled and further purified with HiTrap Heparin HP Agarose column (GE Healthcare), after a five-fold dilution step ...
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bioRxiv - Biophysics 2024Quote: ... Protein was further purified by size-exclusion chromatography using a Superdex 200 column (GE healthcare) in SEC buffer (25 mM Tris ...
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bioRxiv - Biophysics 2024Quote: ... Pooled fractions were loaded onto a 5 ml Q-HP column (GE Life Sciences) equilibrated in buffer A and washed with 5% buffer B ...
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bioRxiv - Biophysics 2024Quote: ... and impurities were removed using a Superdex 75 column (GE Life Sciences). Protein pellets were resuspended in 4 ml of TBS buffer with protease inhibitor and further purified by size-exclusion chromatography on a Sephacryl 26/60 column pre-equilibrated with buffer containing10mM potassium phosphate ...
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bioRxiv - Biophysics 2024Quote: ... The filtered supernatant was then incubated at 4 ºC with gentle shaking at 5 rpm for 1 hour in the presence of 5 mL of HisPur Cobalt Resin (GE Healthcare). After multiple rounds of resin cleaning using Extraction Buffer supplemented with 5 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... NF-κBRHD and NF-κBFL were purified by cation exchange chromatography (MonoS; GE healthcare) to remove bound nucleic acids ...
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bioRxiv - Biophysics 2024Quote: ... The eluent was concentrated and loaded onto Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated with Buffer B (25 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... The protein was eluted with 400 mM imidazole and concentrated and applied to Superdex 200 increase 10/300 GL column (GE Healthcare) equilibrated in 0.2 M sodium bicarbonate buffer (pH 7.5) ...
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bioRxiv - Biophysics 2024Quote: ... The protein eluent was concentrated to 500 µL and subjected to size-exclusion chromatography on a Superdex 200 increase 10/300 GL column (GE Healthcare) equilibrated with Buffer D (25 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... and loaded in 0.5 mL portions onto a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) preequilibrated with size-exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2024Quote: ... Image acquisition was performed using DeltaVision Ultra Image Acquisition software and image processing was performed using softWoRx software (Applied Precision). Images were deconvolved for 5 cycles using the conservative setting ...
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bioRxiv - Cell Biology 2024Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer and 0.150 M NaCl) using PD-10 desalting columns or Midi-Trap G-25 (GE Healthcare). The purity of the proteins was evaluated using SDS–PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Cell Biology 2024Quote: ... followed by detection with ECL Prime Western Blot Detection (GE Healthcare). Blots were imaged in a Odyssey®CLx system /LI-COR).
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bioRxiv - Cell Biology 2024Quote: ... Quantification of the signals was performed using ImageQuant TL (GE Healthcare).
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bioRxiv - Biophysics 2024Quote: ... The different phosphorylation states of PKA-CF100A were separated using a cation exchange column (HiTrap Q-SP, GE Healthcare Life Sciences) using a linear gradient of KCl in 20 mM KH2PO4 at pH6.5.83 The purified protein isoforms were then stored in phosphate buffer containing 10 mM dithiothreitol (DTT) ...
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bioRxiv - Biophysics 2024Quote: All SPR binding experiments were performed on a CM5 chip (Cytiva) at 25 °C using a Biacore T200 Instrument (GE Healthcare). The purified CNBH domains were immobilized on the CM5 chip using a standard amine coupling chemistry in the presence of 10 mM sodium acetate buffer at pH 5.5 as the immobilization buffer (buffer used to directly dissolve ligands) ...
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bioRxiv - Biophysics 2024Quote: ... Cleaved ProTα was purified further using Ni Sepharose Excel resin (Cytiva, formerly GE Healthcare; Søborg, Denmark) and a HiPrep Q FF column (Cytiva ...
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bioRxiv - Cancer Biology 2024Quote: ... 24 cm (GE Healthcare Bio-Sciences, Piscataway, NJ) was used ...
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bioRxiv - Cancer Biology 2024Quote: Surface plasmon analysis was performed using the Biacore-T-200 system (GE Healthcare). Both recombinant PRMT5-MEP50 complex and EZH2 (50µg ...
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bioRxiv - Cell Biology 2024Quote: ... which was probed with a horseradish peroxidase-coupled secondary antibody (donkey-α-rabbit IgG, GE HealthCare, 1:400). Subsequent staining was performed using 3-amino-9-ethylcarbazol ...
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bioRxiv - Cell Biology 2024Quote: ... or HRP-conjugated donkey anti-mouse antibody (catalog number NXA931; GE Healthcare) or HRP-conjugated donkey anti-goat antibody (catalog number sc-2020 ...
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bioRxiv - Cell Biology 2024Quote: ... After incubation with peroxidase-conjugated secondary antibodies (GE Healthcare), the signals were detected using ImmunoStar LD (Fujifilm Wako).
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bioRxiv - Cell Biology 2024Quote: ... All blots were probed with an HRP-conjugated donkey anti-rabbit secondary antibody (catalog number NA934V; GE Healthcare) or HRP-conjugated donkey anti-mouse antibody (catalog number NXA931 ...
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bioRxiv - Cell Biology 2024Quote: ... and subjected to SDS-PAGE before transfer to nitrocellulose (GE Healthcare). Membranes were blocked in PBS containing 5% milk (PBS-M ...
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bioRxiv - Cancer Biology 2024Quote: ... Amersham™ ECL™ Prime Western blotting Detection Reagent (GE Healthcare, Cat. #RPN2232) on a Chemidoc™ MP Imaging System (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... equipped with 60x/1.30 NA Sil or 20x/0.85 NA oil objective lens (OLIMPUS) and a cold CCD camera (CoolSNAP HQ; Roper Scientific) that was controlled using Delta Vision SoftWorx (Applied Precision). The Z-stack images were then deconvoluted.
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bioRxiv - Cell Biology 2024Quote: ... Glutathione– sepharose 4B was from GE Healthcare (Piscataway ...
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bioRxiv - Cell Biology 2024Quote: Live imaging experiments were carried out on the DeltaVision Elite microscope (GE healthcare) or the CSU-W1 SpinningDisk (Nikon ...
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bioRxiv - Cell Biology 2024Quote: ... and chemiluminescence films (Amersham Hyperfilm ECL, GE Healthcare). Protein levels were quantified using Fiji (RRID:SCR_002285 ...
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bioRxiv - Cell Biology 2024Quote: Micrographs acquired on the Applied Precision DeltaVision microscope were deconvolved using an iterative algorithm in SoftWoRx (6.5.1; Applied Precision, GE Healthcare). Unprocessed images were used for all quantification of fluorescence intensity on micrographs acquired on the DeltaVision ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with blocking solution (2.5 % FBS, 200 mM glycine, 0.1 % Triton X-100 in PBS or 2 % bovine serum albumin (GE Healthcare or Calbiochem), 0.01 % Tween 20 (National Diagnostics or Roth ...