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Citations for GE Life Sciences :
1051 - 1100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... eluted protein was loaded onto a HiPrep 26/10 desalting column (GE Healthcare) containing 25 mM Tris pH 7.5 at 20 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The density of the bands corresponding to concerted integration on fluorescence-scanned gels was quantified by ImageQuant (GE Healthcare). The integration activity of WT IN was set to 100% ...
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bioRxiv - Biochemistry 2024Quote: ... DNA was visualized by fluorescence using a Typhoon 8600 fluorescence scanner (GE Healthcare). Densitometry was used to quantify IN activity ...
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bioRxiv - Biochemistry 2024Quote: ... Multimeric state of WT and mutant INs were analyzed on Superdex 200 10/300 GL column (GE Healthcare) equilibrated with running buffer at a flow rate of 0.2 ml/min.
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bioRxiv - Biochemistry 2024Quote: ... The reaction mix was loaded onto a HisTrap column (GE Healthcare) equilibrated with 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: The gels were scanned with an Amersham Typhoon RGB Biomolecular Imager (GE Healthcare Life Sciences) to visualize the fluorophore-labeled primer and extended primer bands ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Biochemistry 2024Quote: ... Autoradiography was performed with an Amersham Typhoon 5 Biomolecular Imager (GE Healthcare Life Sciences), and images were quantified using ImageQuant TL software (Amersham Biosciences) ...
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bioRxiv - Biochemistry 2024Quote: ... hTIGIT-ECD in fusion with a C-terminal Fc tag (Acro Biosystems, Cat. No. TIT-H5254) was immobilized on CM5 sensor chips (GE Healthcare) in 10 mM sodium acetate ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µL of a 1:1 mix of two types of commercially available carboxylate beads (Sera-Mag Speed beads, 45152105050250, 65152105050250, GE Healthcare), and 60 µL ethanol ...
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bioRxiv - Bioengineering 2024Quote: ... 1 ml antibody-containing ascites was incubated with 1.2 ml settled bead volume of pre-equilibrated Protein G Sepharose Fast Flow beads (GE Healthcare) for 2 hr at 4°C with gentle agitation ...
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bioRxiv - Biochemistry 2024Quote: ... The middle two layers were removed for subsequent desalting (using five connected Hi-Trap desalting columns (GE Healthcare, Chicago, IL, USA) on an AKTA Pure FPLC system) ...
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bioRxiv - Bioengineering 2024Quote: ... and transferred onto a nitrocellulose membrane (Amersham Protan, GE Healthcare, #10600012). Proteins were visualized by ECL western blotting detection reagents (Pierce ...
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bioRxiv - Biochemistry 2024Quote: ... Purification and fractionation steps were performed with an “ÄKTApurifier” FPLC system (GE Healthcare). The filtrate was loaded onto a HiTrap DEAE FF column (5 mL ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM 2-mercaptoethanol and desalted into the same buffer using a 5 mL HiTrap desalting column (GE Healthcare) using an AKTA FPLC system (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... HiLoad16/600 column (GE Healthcare). Elution fractions containing pure protein were determined via SDS-PAGE analysis on a 4-20% gradient gel (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... The eluate was concentrated to a total volume of 2.5 mL and desalted using PD-10 desalting columns (GE Healthcare) and desalting buffer (25 mM Tricine-NaOH ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... Proteins were further purified by concentrating to ∼3mg/ml and applying to a size exclusion column (Superdex-200 increase, 10/30 GE Healthcare) equilibrated in buffer A containing 5 mM ATP ...
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bioRxiv - Biochemistry 2024Quote: ... The concentrated sample was then subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), in buffer containing 150 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... protein was loaded onto a pre-equilibrated (50 mM Tris-HCl 7.5 at 4 °C and 100 mM sodium chloride) Superdex 16/600 size exclusion column (GE Life Sciences, Chicago, IL) following Ni-NTA affinity chromatography to remove any protein aggregate ...
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bioRxiv - Biochemistry 2024Quote: ... The ultracentrifuged sample was purified by size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), equilibrated with buffer containing 20CmM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... the membrane was treated with secondary ECL anti-rabbit or anti-mouse IgG HRP-linked antibodies (GE Healthcare, Pittsburgh, PA) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-231 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (cat# SH30243, Hyclone, GE Healthcare Life Sciences, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Western blot detection was performed with secondary antibodies conjugated with horseradish peroxidase (rabbit (GE Healthcare) or mouse (Sigma-aldrich)) ...
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bioRxiv - Biophysics 2024Quote: ... filtered (0.42 pore size) and subsequently loaded onto a HiTrapQHP anion exchange chromatography column (GE Healthcare). The protein was eluted using a gradient with 25 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... The secondary antibodies used were a sheep α-mouse antibody conjugated to horseradish peroxidase (HRP) (GE Life sciences, Marlborough MA) at a 1:10,000 dilution or a donkey α-rabbit antibody conjugated to HRP (GE Life sciences ...
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bioRxiv - Cell Biology 2024Quote: ... Separated proteins were transferred to a nitrocellulose membrane (GE Healthcare, Freiburg, Germany) in transfer buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... and the images used to generate the data in Figure 3D were acquired on a DeltaVision microscope (Applied Precision) equipped with a UPlanSapo x100 1.4 numerical aperture oil immersion objective (Olympus) ...
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bioRxiv - Biophysics 2024Quote: ... at a 1:10,000 dilution or a donkey α-rabbit antibody conjugated to HRP (GE Life sciences, Marlborough MA) at a 1:10,000 dilution ...
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bioRxiv - Biophysics 2024Quote: ... 1% Antibiotic Antimycotic Solution (GE Healthcare Life Sciences), and 0.5 mg/mL of the aminoglycoside antibiotic G418 (Invitrogen) ...
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bioRxiv - Biophysics 2024Quote: ... 300 mM imidazole and 0.6% C8E4 (tetraethylene glycol monooctyl ether) and concentrated before loading onto a Superdex 200 increase 10/300 GL column (GE Healthcare) equilibrated with buffer G containing no imidazole ...
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bioRxiv - Biophysics 2024Quote: ... The resulting suspension was then centrifuged (13,000 rpm for 35 min at 4°C) and the supernatant was applied to a HisTrap HP column (GE Healthcare) previously equilibrated with the lysis buffer ...
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bioRxiv - Biophysics 2024Quote: ... pure fractions were buffer-exchanged into the final buffer (20 mM Tris-HCl, pH 8, and 150 mM NaCl) using a HiPrep Desalting column (GE Healthcare) and stored at -20°C in the final buffer ...
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bioRxiv - Bioengineering 2024Quote: Single-cycle kinetics analysis of CD4-Ig binding to the 16055-ConM-v8.1 SOSIP with C-terminal Spy-tag-2 (ST2) was performed at 25°C on a Biacore T100 (GE Healthcare). 1X HBS-EP containing 10 mM HEPES ...
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bioRxiv - Bioengineering 2024Quote: ... Gadodiamide (GdDTPA-BMA, Omniscan®, GE Healthcare Inc. Marlborough, MA) MRI contrast was purchased through the OHSU in-patient pharmacy ...
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bioRxiv - Bioengineering 2024Quote: ... and loaded onto a HisTrap HP 5 ml column (GE Healthcare) at 4 °C using an ÄKTA Pure 25 M (GE Healthcare) ...
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bioRxiv - Bioengineering 2024Quote: Surface plasmon resonance measurements were performed using a NTA chip on a Biacore X100 SPR system (GE Healthcare) according to manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2024Quote: ... The kinetic parameters were calculated by using the curve-fitting facility of the BIAevaluation version 4.1.1 software (GE healthcare) and the simple 1:1 Langmuir binding model (A + B ↔ AB).
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bioRxiv - Bioengineering 2024Quote: ... and applied to Ni-NTA column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Bioengineering 2024Quote: Surface plasmon resonance (SPR) experiments were performed using a BIAcore 3000 (GE Healthcare). The ligand protein was immobilized on a CM5 sensor chip using amine coupling method ...
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bioRxiv - Bioengineering 2024Quote: ... The soluble fraction was separated by centrifugation at 30,000×g for 30 min and subjected to size exclusion chromatography using a HiLoad26/60 Superdex75 column (GE Healthcare) with gel filtration buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: ... or G12p4 antibodies were immobilized on a CM5 sensor chip (GE Healthcare Technologies) with an amine-coupling kit according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Kinetic analyses were performed at 25°C using a BIAcore 3000 machine (GE Healthcare Technologies). A2p1 ...
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bioRxiv - Bioengineering 2024Quote: ... concentrated and applied to a Superose6 GL gel filtration column (GE Healthcare) equilibrated with gel filtration buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: ... The oligomeric state and purity were determined at 25°C by size-exclusion chromatography on a Superdex 200 10/300GL column (GE Healthcare Technologies, Chicago, IL).
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bioRxiv - Biophysics 2024Quote: ... and purified with HisTrapTM HP 5ml column on an Akta Purifier FPLC instrument with gradients (GE Healthcare Life Sciences). The clarified cell lysate was loaded on the column equilibrated with buffer A (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was removed and filtered through a 0.22 μM filter and loaded onto a HisTrap High Performance column (GE Healthcare) equilibrated with buffer A containing 5 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: The yeast tRNAPhe from Sigma was labeled on its dihydrouridine (D) residues at the 16/17 positions using Cy3-hydrazide from GE Healthcare. This process involved first reducing the D residues with sodium borohydride (NaBH4 ...
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bioRxiv - Bioengineering 2024Quote: ... sonicated and purified by nickel affinity followed by size exclusion chromatography (Superdex™ 75 10/300GL or Superdex™ 75 10/300GL Increase GE Healthcare). All protein samples were characterized by SDS-PAGE ...
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bioRxiv - Bioengineering 2024Quote: ... Cell imaging was performed utilizing the InCell Analyzer 2500 (GE Healthcare) microscope with the 20x objective across three channels ...