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Citations for GE Life Sciences :
1001 - 1050 of 2313 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Proteins were purified by nickel-affinity chromatography and samples were passed over a HiLoad® 16/600 Superdex® 75 or 200 pg column (GE Healthcare), equilibrated in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was injected into a Ni2+-NTA agarose affinity column (containing 5 mL Ni2+-Sepharose 6 FF beads, GE Healthcare, Cat. 17531803). The column was incubated for 15 minutes and washed twice with 30 mL washing buffer (50 mM Tris ...
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bioRxiv - Plant Biology 2021Quote: The binding kinetics and affinities of MIK2ECD with SCOOP12 or SCOOP12SS/AA peptides were assessed on a Biacore T200 instrument (GE Healthcare, Pittsburgh, PA) with CM5 sensor chips ...
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bioRxiv - Biophysics 2022Quote: ... Purification of resulting protein was performed by immobilized metal affinity chromatography using a 5 mL HisTrap HP column (GE Healthcare, Frei-burg, Germany) containing HisTrap chelating stationary phase (Amersham Biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... The crude extract was applied to an immobilized metal ion affinity chromatography column (HisTrap FF, 5 mL resin; GE Healthcare, Little Chalfont, UK). After washing with 25 mL of the 1 x T-buffer containing 25 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... according to manufacturer’s protocol and removed the FLAG tag and His6-tag with TEV digestion followed by reverse nickel affinity purification and sizeexclusion chromatography on a Superose 6 Increase column (GE Healthcare Life Sciences). We biotinylated the protein with BirA ligase as previously described32.
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bioRxiv - Immunology 2020Quote: ... and purified with PGT145 affinity chromatography followed by size exclusion chromatography (SEC) using a HiLoad® 16/600 Superdex® pg200 (GE Healthcare) as described previously 53.
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bioRxiv - Biochemistry 2020Quote: ... These affinity-purified protein concentrates were then applied to a previously equilibrated HiLoad 16/600 Superdex 200 pg column (GE Healthcare Life Sciences) packed with dextran covalently bound to highly cross-linked agarose ...
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bioRxiv - Cell Biology 2022Quote: ... and expressed proteins were purified from the lysis supernatant by Nickel affinity and gel filtration (Superdex 75 26/60, GE Healthcare, Chicago, IL) chromatography ...
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bioRxiv - Cell Biology 2019Quote: ... Purification was achieved by immobilized metal affinity purification followed by size exclusion chromatography (SEC) on an ÄktaPurifier Chromatography System (GE Healthcare, Buckinghamshire, UK).
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bioRxiv - Molecular Biology 2019Quote: ... Flow through containing the unbound ID2 complex was diluted to 150 mM NaCl concentration and loaded onto a HiTrap Heparin HP affinity column (GE Healthcare Life Sciences). Using a shallow NaCl gradient ...
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bioRxiv - Molecular Biology 2019Quote: ... Collected fractions containing FANCI were diluted to ∼100 mM NaCl concentration and loaded onto a HiTrap Heparin HP affinity column (GE Healthcare Life Sciences). Using a shallow NaCl gradient ...
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bioRxiv - Molecular Biology 2019Quote: ... The elution was then diluted to ∼100 mM NaCl concentration and loaded onto a HiTrap Heparin HP affinity column (GE Healthcare Life Sciences). Using a shallow NaCl gradient ...
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bioRxiv - Synthetic Biology 2019Quote: ... Clarified supernatants were then purified using His-tag affinity chromatography with a Ni-NTA column (HisTrap FF 5mL column, GE Healthcare Life Sciences) followed by size exclusion chromatography (Superdex HiLoad 26/600 200 pg column ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies were affinity-purified from filtered cultured supernatant on a High-Trap protein G column (GE Healthcare, Chicago, USA, Cat.Nr 17-0404-01).
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bioRxiv - Synthetic Biology 2020Quote: ... The lysate was then subjected to nickel-immobilized metal affinity chromatography (IMAC) using a HisPrep™ Fast Flow 16/10 column (GE Healthcare, USA) equilibrated with equilibration buffer (20 mM NaH2PO4 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filtered (0.45 μm) and the target protein purified via immobilized metalion affinity chromatography (IMAC) using a 5 mL HisTrap™ HP column (GE Healthcare, UK). The lysis buffer (50 mM Tris/HCl ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified supernatant containing TetR was then purified using His-tag affinity chromatography with a Ni-NTA column (HisTrap FF 5mL column, GE Healthcare Life Sciences) followed by size exclusion chromatography (Superdex HiLoad 26/600 200 pg column ...
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bioRxiv - Immunology 2020Quote: ... and BG505 SOSIP.v5.2 were expressed in HEK293F cells and purified with either PGT145 or 2G12 affinity chromatograph followed by size exclusion chromatography (SEC) using a HiLoad® 16/600 Superdex® pg200 (GE Healthcare) as described previously 7 ...
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bioRxiv - Biochemistry 2020Quote: ... This cell supernatant was then loaded into protein A affinity column (HiTrap protein A high performance, GE29-0485-76; GE Healthcare Life Sciences,) using liquid chromatography system (GE AKTA Prime Plus) ...
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bioRxiv - Bioengineering 2022Quote: ... dialysed into a Tris-saline buffer (Tris 10 mM, NaCl 150 mM, pH 7.4) then affinity purified on a protein A sepharose column (GE Healthcare, Chicago, IL, USA). Bound antibody was eluted with 0.1 M Glycine pH 2.4 and neutralised with 1 M Tris pH 8.5 ...
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bioRxiv - Plant Biology 2022Quote: ... HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare, Chicago, IL) at 4 °C for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... Bovine rhodopsin can be further isolated by detergent solubilisation and affinity chromatography using a Concanavalin A resin (ConA, GE Healthcare Life Sciences, USA) as described in Edwards et al ...
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bioRxiv - Microbiology 2022Quote: The affinity of the sE protein for the pr peptide was measured by SPR using a Biacore T200 system (GE Healthcare Life Sciences) equilibrated at 25ºC ...
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bioRxiv - Neuroscience 2022Quote: ... were purified from bacterial lysate by affinity chromatography on a GSTrap FF column (Cat. No. 71-5016-96 AM, GE Healthcare, Chicago, IL) followed by dialysis against multiple changes of phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted protein from this affinity step was concentrated and further purified by gel filtration on a HiLoad® 16/600 Superdex® 200 pg column (GE Healthcare) equilibrated in 20 mM TrisHCl ...
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bioRxiv - Neuroscience 2022Quote: ... The stabilized Q-SNARE complex was purified by Ni2+-NTA affinity chromatography followed by ion-exchange chromatography on a Mono Q column (GE Healthcare, Piscataway, NJ) in the presence of 50 mM n-octyl-β-D-glucoside (OG)10 ...
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bioRxiv - Neuroscience 2023Quote: ... The adeno-associated virus (AAV) vectors were produced by helper-free triple transfection and purified using affinity chromatography (GE Healthcare, Chicago, IL, USA). For production of AAV2.1 vector ...
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bioRxiv - Biophysics 2023Quote: ... His6-tagged fusion proteins were isolated from the resulting supernatant by affinity chromatography using Ni2+ Sepharose High-Performance beads (GE Healthcare, Pittsburgh, PA). Bound fusion proteins were washed with 200-300 mL of column Buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... and 6xHis-SMT3/SUMO tagged IMPDH2 was initially purified by Ni affinity chromatography using either a HisTrap FF column (GE Healthcare Life Sciences) on an Äkta Start chromatography system or a handpacked HisPur™ Ni-NTA resin (Thermo Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... The pellet was added to lysed HEK cells prior to virus purification through heparin affinity columns (HiTrap Heparin HP, GE Healthcare, Uppsala, Sweden) and concentrated using Amicon Ultra-4 centrifugal filters (Millipore ...
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bioRxiv - Bioengineering 2024Quote: ... sonicated and purified by nickel affinity followed by size exclusion chromatography (Superdex™ 75 10/300GL or Superdex™ 75 10/300GL Increase GE Healthcare). All protein samples were characterized by SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: GST and GST-Rheb were expressed in BL21 cells and purified by affinity chromatography using Glutathione Sepharose High Performance beads (GE Healthcare Life Sciences) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Images were captured by Photometrics Cascade II EMCCD camera and processed by SoftWoRx imaging software (Applied Precision). For counting hooks ...
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bioRxiv - Microbiology 2019Quote: ... Isoelectric focusing was carried out in Multiphor II electrophoresis system (GE Healthcare) in three running phase (phase 1 ...
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bioRxiv - Bioengineering 2023Quote: ... and supplemented with 5 % Cytiva HyClone Fetal Clone II (GE Healthcare Lifesciences), MITO+ (1:1,000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... The protein sample was exchanged into low salt buffer (20 mM Tris pH 8, 20 mM NaCl, 0.03% DDM) using a PD-10 column (GE Healthcare) and then injected onto a MonoQ column (GE Healthcare ...
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bioRxiv - Pathology 2022Quote: ... Low-molecular weight RNAs were separated in 15 % urea denaturing gels and transferred to Hybond-NX membranes (GE Healthcare, UK) for hybridization with biotin-labeled DNA oligo probes and detectiond by Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Scientific ...
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bioRxiv - Biophysics 2022Quote: ... hSERINC3 was eluted in low salt buffer contain 2.5 mM desthiobiotin and then concentrated using a centrifugal filter (Vivaspin, 50 kDa MWCO (GE Healthcare)) ...
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bioRxiv - Biochemistry 2020Quote: ... ACE2-Fc proteins were then purified by size-exclusion chromatography (SEC) using Superdex 200 Increase column (GE healthcare, Chicago, IL) and stored at −80°C until used in experiments.
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bioRxiv - Molecular Biology 2021Quote: ... The VHH72-Fc constructs were purified from the clarified culture medium using a 5 ml MabSelect SuRe column (GE Healthcare). After a wash step with McIlvaine buffer pH 7.2 ...
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bioRxiv - Cell Biology 2020Quote: ... Human RAB34 cDNA was obtained from GE Healthcare (MHS6278-202807876) and cloned into Gateway pENTR plasmid pDONR221 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by Ficoll-Paque PLUS (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: ... human C5b was immobilised on a CM5 chip (GE Healthcare). Flow cells were activated using a standard immobilisation protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The solution was dialyzed against Buffer C overnight at 4 °C and applied on a Resource Q column (GE Healthcare) that was equilibrated with Buffer C ...
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bioRxiv - Cell Biology 2020Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Cell Biology 2019Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Bioengineering 2024Quote: Single-cycle kinetics analysis of CD4-Ig binding to the 16055-ConM-v8.1 SOSIP with C-terminal Spy-tag-2 (ST2) was performed at 25°C on a Biacore T100 (GE Healthcare). 1X HBS-EP containing 10 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... onto a Hybond-C extra membranes (GE Healthcare). Membrane strips were blocked with 3% fatty acid-free BSA (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... at 25°C using Biacore T200 (GE Healthcare). The 6×His-tagged AVR-PikD (ligand ...