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Citations for GE Life Sciences :
1051 - 1100 of 2313 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... at 20 °C in HBS-EP+ (GE Healthcare) running buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was Hybond-C nitrocellulose (GE Healthcare), and transfer was performed in 25 mM Tris ...
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bioRxiv - Genomics 2023Quote: ... transferred onto Hybond C nitrocellulose membrane (GE Healthcare), probed with primary antibodies anti-RRM diluted 1:300 ...
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bioRxiv - Microbiology 2021Quote: ... The second antibodies used for ELISA were as follows: HRP-linked anti-whole rabbit IgG donkey serum (GE Healthcare, #NA934) for detection of the positive control ...
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bioRxiv - Plant Biology 2019Quote: ... The fusion protein was isolated from soluble cell lysate by Co-NTA affinity chromatography with two His-Trap 1 mL TALON Crude columns (GE Healthcare Life Sciences, 28953766). Chromatography buffers contained sodium phosphate 20 mM pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... The pooled and dialyzed protein solution from the metal chelate affinity chromatography was loaded on a 120 ml HiLoad® 16/600 Superdex® 75 pg column (GE Healthcare, Germany). The column was equilibrated in buffer (20 mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... 75-90 μl of affinity-purified protein sample was loaded onto a Superdex 200 Increase 10/300 GL SEC column (GE Healthcare, Chicago, IL, USA) previously equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... using a HiTrap NHS-Activated HP affinity column coupled with aIL-4 monoclonal antibodies and an ÄKTA Fast Protein Liquid Chromatography (FPLC) instrument (GE Healthcare, Piscataway, NJ, USA). Immunizations ...
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bioRxiv - Bioengineering 2020Quote: Binding affinity characterizations of transcribed RNA biosensors were performed using a SPR assay on the Biacore X100 instrument from GE Healthcare (Chicago, IL, USA) as previously described8 ...
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bioRxiv - Biochemistry 2021Quote: Nickel Immobilised Metal Ion Affinity chromatography (Ni2+-IMAC) for protein purification was conducted using a HisTrap® HP 5 mL column (GE Healthcare, Munich, Germany) coupled to a BioLogic DuoFlow™ Chromatography System (Bio-Rad ...
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bioRxiv - Biophysics 2022Quote: ... were purified by immobilized metal affinity chromatography (IMAC) using a HisTrap HP 5 mL column mounted on an Äkta Purifier System (GE Healthcare, Chicago, IL, USA). Fractions containing the His6-Sumo-peptide fusion in elution buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... The protein was purified by nickel affinity chromatography and subsequently by gel filtration on a Hi load 26/60 Superdex-200 column (GE Healthcare, Milwaukee, WI, USA) equilibrated with 20mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified in two steps by immobilized metal chelate affinity chromatography (IMAC) using 5 mL HisTrap FF columns (GE Healthcare, Chicago, Illinois, USA) as previously described ...
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bioRxiv - Molecular Biology 2021Quote: ... Then 6X-His-tagged wild type SufB precursor and mutant proteins were purified by Ni-NTA affinity column (Ni-NTA His trap, HP GE Healthcare Life Sciences-17524802) (23 ...
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bioRxiv - Microbiology 2023Quote: ... aMCFCA was then purified from the supernatant using the AKTA protein purification system through a Ni-NTA HisTrap affinity column (GE Healthcare GE17-5248-02) with loading buffer (10 mM Tris-HCl pH 8.5 ...
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bioRxiv - Bioengineering 2022Quote: ... the receptor fragment MET567 comprising the N-terminal SEMA domain and the cysteine-rich/PSI domain was immobilized at low-density (515 RU) using amine-coupling on a CM5 sensor chip (GE Healthcare). A blank inactivated reference channel was used to correct for non-specific binding ...
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bioRxiv - Immunology 2020Quote: ... CR2-Fc and FH19-20 were prepared in 10 mM sodium acetate pH 5 and immobilised at 300 RU (CR2-Fc: 20 µM, FH19-20: 240 µM) to different flow cells of CM5 chips (GE Healthcare) using standard amine coupling involving preparation of the dextran matrix with 100 mM N-hydroxysuccinimide (NHS ...
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bioRxiv - Biophysics 2020Quote: ... The Anti-hIgG Fc (AHC) capture biosensors were purchased from ForteBio (California, USA) and the protein A coated chips were purchased from GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2022Quote: ... The Fab fragments are then separated from non-digested IgG and Fc fragments by a 1 hr incubation with rProtein A Sepharose Fast Flow (GE Healthcare). Post incubation ...
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bioRxiv - Microbiology 2020Quote: ... the VHH-72-Fc protein in the cleared cell culture medium was captured on a 5 mL MabSelect SuRe column (GE Healthcare), eluted with a McIlvaine buffer pH 3 ...
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bioRxiv - Cell Biology 2020Quote: ... a total of 1×104 RAW 264.7 (ATCC) cells were seeded per well on 24-well plates in 500 μl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies were expressed by transient transfection in Expi-CHO cells as murine IgG2a Fc domain conjugates and purified using the HiTrap MabSelect SuRe (GE Healthcare) affinity chromatography ...
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bioRxiv - Immunology 2023Quote: ... We separated pFab and Fc from undigested IgG using size exclusion chromatography with a Superdex 200 Increase 10/300 column (GE Healthcare) and concentrated the pFab/Fc.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Germany) were grown in RPMI 1640 medium containing 10% heat-inactivated fetal calf serum (FCS, GE Healthcare, Freiburg, Germany or Merck) at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2019Quote: ... and labelled using a standard Rediprime II Random Prime labelling system (GE healthcare) and radioactive [α-32P] dCTP ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... in an Ethan IPGphor II Isoelectric Focusing System (GE Healthcare Life Sciences, USA) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... (Manassas, VA) and Cytiva HyClone Fetal Clone II was purchased from GE Healthcare Life Sciences.
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bioRxiv - Cell Biology 2023Quote: ... Pol II was bound to protein G Sepharose Fast Flow beads (GE Healthcare) containing 8WG16 antibody against Rpb1 ...
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bioRxiv - Cell Biology 2022Quote: Human peripheral blood mononuclear cells were isolated by Ficoll (GE Healthcare) density gradient centrifugation and then stained with PE anti-CD3 (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... human or rabbit IgG (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) were used and HRP activity detected using ECL plus (GE Healthcare Bio-Sciences Corp. ...
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bioRxiv - Immunology 2019Quote: ... Cells were harvested and lysed in PBS supplemented with 1 mM PMSF and soluble protein was purified using nickel-affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare, Piscataway, New Jersey, USA). After SDS-PAGE analysis ...
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bioRxiv - Immunology 2019Quote: ... IgG and IgM antibodies were purified from plasma samples by affinity chromatography using prepacked HiTrap™ Protein G HP columns (GE Healthcare, Wauwatosa, WI, USA) and HiTrap™ IgM Purification HP columns (GE Healthcare ...
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bioRxiv - Systems Biology 2021Quote: ... Samples were centrifuged at 16,000 x g for 20 minutes and the supernatant used for affinity purification of biotinylated proteins using 25 µl of washed streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301) for 16 hours with rotation at 4℃ ...
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bioRxiv - Biophysics 2022Quote: ... TIA1 LC domain was isolated from the supernatant using a Bio-Rad NGC Discover 10 chromatography system and a 5 mL Ni-affinity column (Bio-Rad Bio-scale Mini Nuvia IMAC or GE Healthcare HisTrap FF crude). The column was equilibrated in 500 mM sodium chloride ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatants were filtrated and subjected to affinity chromatography using a 5 mL HisTrap HP His-tag purification column (GE healthcare, cat. # GE29-0510-21) operated by a peristaltic pump (Cytiva ...
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bioRxiv - Microbiology 2020Quote: ... the removal of SUMO protease and SUMO tag from the cleavage reaction was performed with affinity chromatography on a HisTrap HP column (GE Healthcare, Code#:17-5248-01). The SUMO protease and SUMO tag will stay in the HisTrap HP column and the cleaved native protein will be in the flow-through fractions ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were first purified with affinity chromatography using AKTA pure 25 system and HiTrap MabSelect SuRe protein A column (GE healthcare, Cat# 11-0034-94) for huACE2-IgHu-pVax ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then collected by vacuum filtration onto glass microfiber filters (grade GF/C, 125 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 50 mL centrifuge tubes (Sarstedt ...
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bioRxiv - Cell Biology 2022Quote: ... Extract was cleared by centrifugation at 186.000 x g for 1 h at 4°C and then incubated at 4°C with Glutathione Sepharose™ 4B resin (GE Healthcare) for 3h ...
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bioRxiv - Neuroscience 2021Quote: ... Lipofectamine 2000 and plasmidic DNA were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
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bioRxiv - Genomics 2020Quote: ... The MyoD-FL protein in flow through was diluted with pre-cooling 20 mM Tris-HCl pH 8.0 to ensure a low NaCl concentration to remove excess nucleic acids by HiTrap SP FF column (GE Healthcare, Beijing, China) with buffer A containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... Lipofectamine 2000 and siRNAs were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
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bioRxiv - Biochemistry 2021Quote: Low molecular weight (LMW) screening with the DSF fragment hits was carried out using the T200 Biacore instrument (GE Healthcare Life Sciences). A series S CM5 sensor chip (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions with low protein background were pooled and subjected to size-exclusion chromatography on a Superdex 75 pg column (GE Healthcare, USA) in the storage buffer (DesAOMT ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was then diluted to a final salt concentration of low salt buffer conditions (100 mM KCl, 50 mM HEPES, 1 mM MgCl2) and bound to HiTrap SP FF (GE Healthcare, USA) anion exchange column ...
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bioRxiv - Cell Biology 2020Quote: ... 28°C and purified on HisTrap columns (GE Healthcare) by Imidazole gradient elution on AKTA Prime according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C onto 0.2uM PVDF membranes (GE Healthcare Biosciences). After transfer ...
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bioRxiv - Genomics 2024Quote: ... transferred onto a Hybond-C nitrocellulose membrane (GE Healthcare), then blocked with TBST containing 5% (w/v ...
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bioRxiv - Immunology 2019Quote: ... followed by horseradish peroxidase (HRP)-labeled secondary antibody goat-anti-mouse IgG Fc (Pierce Antibodies) or donkey-anti-rabbit IgG (GE Life Sciences). HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).