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Citations for GE Life Sciences :
1201 - 1250 of 2313 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... SPR measurements were performed at 25 °C using a Biacore T200 apparatus (GE Healthcare) and 50 mM HEPES-NaOH pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... cation exchange gel and SP Sephadex C-25 (GE Healthcare Bio-Sciences AB, Sweden) strong cation exchange particles ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... and cleaved from GST resin overnight at 4°C with PreScission Protease (GE Healthcare). GST was affinity purified as described above and eluted using 15 mM reduced L-gluathione ...
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bioRxiv - Biophysics 2019Quote: Human SH-SY5Y neuroblastoma cells (ATCC, Manassas, VA) were cultured in DMEM/F12 (GE Healthcare, Boston, MA) with 10% fetal bovine serum (Gibco Co. ...
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bioRxiv - Cell Biology 2021Quote: A commercially available siRNA pool was used to knockdown human RNF213 (#M-023324-02, GE Healthcare Dharmacon) in figure 2 ...
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bioRxiv - Cell Biology 2020Quote: ... using siRNA SMARTpool ON-TARGETplus Human UBTD1 (80019) or ON-TARGETplus Non-Targeting Control siRNAs) (GE Healthcare). Lipofectamine 2000 was used for plasmid transfection according to the manufacturer’s instructions (11668 ...
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Biochemistry 2022Quote: ... and human FANCD2 was further purified by gel filtration chromatography on a Superdex 200 column (GE Healthcare) equilibrated with Buffer B containing 200 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Human PBMCs were isolated from the blood by density gradient using Percoll (GE Healthcare, 17-5445-01). PBMCs were washed and resuspended in RPMI 1640 supplemented with 10% FBS.
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors using Ficoll-Paque PLUS (GE Healthcare). CD4 T cells were purified from PBMCs using anti-human CD4+ magnetic beads (Miltenyi ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by density gradient centrifugation using Ficoll-Paque (GE Healthcare, Cat#17-5442-03). B cells were purified by negative selection (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: The cDNA of human full-length SMN and truncations were inserted in pGEX-6P-1 (GE Healthcare) using BamHI and XhoI ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were obtained from buffy coat by density gradient centrifugation in Ficoll–Paque PLUS (GE Healthcare). PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec ...
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bioRxiv - Genomics 2022Quote: ... Dual X-ray absorptiometry (DXA) was performed on the femurs and lumbar vertebrae using the Lunar Piximus II (GE Healthcare) as described previously by Beamer et al57 ...
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bioRxiv - Biochemistry 2021Quote: ... The excess Cu(II) was eliminated by passing the protein through a PD MidiTrap G-25 desalting column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 and 150 mM NaCl.
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bioRxiv - Immunology 2022Quote: ... HLA class-I and -II-associated peptides were isolated using the pan-HLA class I-specific mAb W6/32 and the pan-HLA class II-specific mAb TÜ39 (both in house mouse monoclonal) covalently linked to CNBr-activated Sepharose (GE Healthcare). HLA-peptide complexes were eluted by repeated addition of 0.2% TFA (trifluoroacetic acid ...
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bioRxiv - Microbiology 2022Quote: ... Transfer was performed in an XCell II Blot Module in NuPAGE Transfer Buffer (Thermo Fischer) onto a Hybond P 0.45 PVDF membrane (GE Healthcare). After blocking in 5% BSA or milk powder (Carl Roth ...
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bioRxiv - Biochemistry 2020Quote: Size exclusion chromatography was performed on a quaternary Agilent 1290 Infinity II UPLC system equipped with a SEC column (Superdex Peptide 3.2/300, GE healthcare). 40 μg of the eight protein mixture was loaded and separated at a flow rate of 0.05 ml/min with running buffer (30%ACN ...
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bioRxiv - Microbiology 2022Quote: ... and the obtained supernatant was purified using 0.22 μm filter and passed over a Strep-Tactin II column (GE Healthcare) to collect secreted TraC ...
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bioRxiv - Microbiology 2023Quote: ... Transfer was performed in an XCell II Blot Module in NuPAGE Transfer Buffer (Thermo Fischer) onto a Hybond P 0.45 PVDF membrane (GE Healthcare). After blocking in 5% BSA or milk powder (Carl Roth ...
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bioRxiv - Cell Biology 2019Quote: ... 4°C) and the supernatant was applied to a 5 ml HiTrap column (GE Healthcare) loaded with cobalt(II)-chloride hexahydrate (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... 4°C) and the supernatant was applied to a 5 ml HiTrap column (GE Healthcare) loaded with cobalt(II)-chloride hexahydrate (Sigma#255599) ...
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bioRxiv - Cell Biology 2021Quote: ... Filters were prehybridized for 2 hr at 65°C in Rapid-hyb solution (GE Healthcare): Hybridization was performed overnight in the same buffer containing a 32P-labeled probe ...
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bioRxiv - Genomics 2020Quote: Widefield fluorescence imaging was performed at 20 °C on a DeltaVision Elite system (Applied Precision) equipped with a 100 ×/1.40 NA UPLSAPO oil immersion objective (Olympus) ...
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bioRxiv - Biochemistry 2022Quote: ITC measurements were performed at 4 °C using a MicroCal auto-iTC200 calorimeter (GE Healthcare). Samples were extensively dialyzed against a buffer containing 500 mM NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... SPR was performed at 25°C using Biacore T200 system with CM5 chips (GE Healthcare). HBS-T (10 mM Hepes ...
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bioRxiv - Molecular Biology 2021Quote: ... A second incubation overnight at 4°C with Protein G-magnetic Sepharose beads (GE Healthcare) was used to pull down protein:DNA complexes prior to washing in low salt (120mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The binding study was carried out at 25°C in PBS-P buffer (GE Healthcare). The heparin molecule or fondaparinux at different concentrations were flowed over the immobilized recombinant proteins at a flow rate of 30 μl/min with a contact time of 60 s and a dissociation time of 100 s ...
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bioRxiv - Biochemistry 2020Quote: ITC experiments were carried out at 5°C with a MicroCal iTC200 instrument (GE Healthcare). Each of the ATAD2B bromodomain proteins and histone peptide samples were dialyzed into ITC buffer containing 20 mM NaH2PO4 pH 7.0 and 150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: SPR measurements were carried out at 25°C using a Biacore X instrument (GE Healthcare) as described previously28 ...
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bioRxiv - Genetics 2019Quote: Widefield fluorescence imaging was performed at 20°C on a DeltaVision Elite system (Applied Precision) equipped with a 100 × /1.40 NA UPLSAPO oil immersion objective (Olympus) ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C with Protein G Sepharose 4 Fast Flow (GE Healthcare) overnight ...
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bioRxiv - Neuroscience 2022Quote: Equilibrium binding experiments were performed at 25°C using a Biacore T200 instrument (GE Healthcare) using PBS + 0.005% (v/v ...
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bioRxiv - Microbiology 2023Quote: Antibody binding properties were analysed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 40 min at 4°C with nickel phosphate loaded Sepharose beads (GE Healthcare) to separate the His tag ...
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bioRxiv - Cell Biology 2023Quote: ... equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
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bioRxiv - Cancer Biology 2023Quote: ... and transferred onto PVDF membranes (Hybond-C Extra, GE Healthcare Life Sciences, Mississauga, ON, Canada). Membranes were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... This was incubated at 4°C overnight with 2 units of PreScission protease (GE Healthcare) in cleavage buffer ...
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bioRxiv - Microbiology 2024Quote: ... ITC experiments were performed at 25°C using an ITC-200 MicroCal microcalorimeter (GE Healthcare) following a standard procedure (Hands-Taylor et al ...
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bioRxiv - Bioengineering 2024Quote: Kinetic analyses were performed at 25°C using a BIAcore 3000 machine (GE Healthcare Technologies). A2p1 ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 µL of 8.1 µM human Notch1fe were loaded on a Superose6 10/300 increase column (GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Cancer Biology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh buffy coats using Ficoll-Paque Plus (GE Healthcare) gradient centrifugation ...
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bioRxiv - Immunology 2022Quote: Human PBMCs were isolated by Ficoll gradient purification as per manufactures protocol (Ficoll, GE-17-1440, GE Healthcare) from human peripheral blood leukapheresis pack (StemCell) ...
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bioRxiv - Neuroscience 2020Quote: ... monkey (XP_005545315.1; 121-760aa) and human (NP_001121620.1; 120-760aa) TfR1 in 0.1% BSA in HBS-EP+ buffer (GE Healthcare) was captured at flow rate 10 μl/min ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were isolated from human CMV-seropositive buffy coats by density centrifugation over Ficoll Paque Plus (GE Healthcare) and screened for the presence of NKG2C+ NK cell expansions by flow cytometry 51 ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA encoding human ZIP4 (GenBank access number: BC062625) from Mammalian Gene Collection were purchased from GE Healthcare, and inserted into a modified pEGFP-N1 vector (Clontech ...
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bioRxiv - Molecular Biology 2020Quote: ... The gel was stained in Sybr Green II and scanned on a Typhoon 9410 Variable Mode Imager (GE Healthcare, Piscataway, NJ).
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bioRxiv - Molecular Biology 2019Quote: ... and ii) the final step of the purification involved gel filtration through a Superdex 200 Increase 10/300 GL (GE Healthcare) column in storage buffer (50 mM Tris-Cl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: SEC-MALS experiments were carried out with a DAWN HELEOS II detector (Wyatt Technology) with an in-line size exclusion Superdex 200 (10/300 GL) column (GE Healthcare) balanced in 10 mM phosphate ...
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bioRxiv - Plant Biology 2021Quote: ... Eluted proteins were copper saturated with Cu(II)SO4 and applied to a 120 mL Superdex 75/16 column (GE Healthcare) to remove the excess of copper ...