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Citations for GE Life Sciences :
951 - 1000 of 6458 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Visualization of the stained protein on nitrocellulose membranes was carried out by a LAS-4000 Mini luminescence image analyzer (GE Healthcare, Germany) using the ECL Western Blotting Detection Reagents (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... 20 ug of protein extracts for analysis of MT severing enzymes or 2.5 ug of protein extracts for analysis of tubulin proteins were separated under denaturing conditions and transferred to Amersham Protran Premium 0.45 μm nitrocellulose membranes (GE Healthcare Life Sciences) prior to blocking in 5% non-fat dried milk in TBS-T for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were resolved by SDS-PAGE and proteins were transferred to a nitrocellulose membrane (Cat#10600003, AmershamTM ProtranTM Premium 0.45 μm NC, GE Healthcare Life sciences) which were incubated in blocking buffer (5% nonfat dry milk ...
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bioRxiv - Plant Biology 2022Quote: ... or adjusted to Chl/protein content were fractionated by electrophoresis on SDS-polyacrylamide gels (12%) and blotted onto nitrocellulose membranes (Amersham Protran, GE Healthcare, UK). Membranes were stained with Ponceau S and probed with protein-specific antibodies according to (Sambrook ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein samples were separated on 8% Tris/glycine gels and then transferred to nitrocellulose membranes (GE Healthcare, Uppsala, Sweden). Subsequently the membranes were incubated with primary antibodies and HRP-coupled secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then subjected to SDS-PAGE followed by transfer of the proteins onto nitrocellulose membranes (GE Healthcare, Chalfont St Giles, UK). Membranes were blocked in 3% BSA/PBST (1x PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein extracts were resolved on SDS-PAGE (10% acrylamide) and transferred to a nitrocellulose Hybond ECL membrane (GE Healthcare, Pittsburgh, PA, USA) for probing with high-affinity rat monoclonal antibodies anti-HA (Roche ...
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bioRxiv - Pathology 2024Quote: ... Proteins were separated by SDS-PAGE (10 % polyacrylamide gel) and transferred to a PVDF membrane (RNP303F, GE Healthcare, Little Chalfont, Buckinghamshire, UK) in a semi-dry blotter (TE70X ...
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bioRxiv - Neuroscience 2023Quote: ... and transferred to polyvinylidene difluoride membranes (PVDF) membranes (GE Healthcare Bio-Science). The membranes were blocked with TBS/0.1% Tween-20 (T-TBS ...
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bioRxiv - Biophysics 2020Quote: ... the supernatant was collected for purification using His Trap Excel column (GE Healthcare). The proteins were labeled with an AlexaFluor dye through the ybbR-tag and gel-filtered via a Superdex 75 10/300 GL column ...
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bioRxiv - Cell Biology 2019Quote: ... His-NF1 GAP was purified using a HisTrap excel-affinity column (GE Healthcare) and eluted in buffer containing 50mM Tris ...
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bioRxiv - Microbiology 2020Quote: His-tagged 2019-nCoV S was immobilized to an NiNTA sensorchip (GE Healthcare) to a level of ∼800 response units (RUs ...
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bioRxiv - Neuroscience 2020Quote: ... and a Hi-trap Q HP anion exchange column (GE Healthcare Life Sciences). Fractions containing α-syn were identified by SDS-PAGE and Coomassie staining ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded on a Ni-NTA His-trap column (GE healthcare), washed with 10 column volume of buffer A containing 30 mM imidazole ...
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bioRxiv - Cell Biology 2020Quote: MCAK was first affinity purified by binding to His-Trap HP (GE Healthcare) in binding buffer (50mM NaPO4 ...
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bioRxiv - Molecular Biology 2020Quote: ... and lysates were cycled over a 5 ml His-Trap column (GE Healthcare) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were passed over a 1 ml His-Trap HF column (GE Healthcare). The bound protein was washed with 15 ml high salt wash buffer (20 mM potassium HEPES ...
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bioRxiv - Biophysics 2019Quote: ... further loaded onto a His-Trap HP column (GE Healthcare, Chicago, IL, USA), washed and then eluted in gradient with the same buffer supplemented with 250 mM imidazole pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: IMAC purification was performed using a His-Trap HP 1 ml (GE Healthcare) on an ÄKTA START protein purification system (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was purified by His Trap™ FF crude column (GE Healthcare) which was eluted first by 50 mM imidazole (Innochem) ...
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bioRxiv - Microbiology 2021Quote: ... and applied to a 1-ml chelating Hi-Trap Sepharose column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Neuroscience 2019Quote: ... and a Hi-trap Q HP anion exchange column (GE Healthcare Life Sciences). Fractions containing α-syn were identified by SDS-PAGE and Coomassie staining ...
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bioRxiv - Biochemistry 2021Quote: ... RNase HI was loaded onto a 16/600 Superdex 75 column (GE Healthcare), whilst PolI and Ligase were further purified on a 16/600 Superdex 200 column (GE healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded onto a 3ml His SpinTrap™ column (GE Healthcare) previously charged with Ni2+ and equilibrated in lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... lysate was affinity purified using Cobalt-charged His-Trap Chelating column (GE Healthcare) through imidazole gradient elution ...
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bioRxiv - Immunology 2020Quote: ... Harvested supernatants were applied to a Hi-Trap Nickel excel columns (GE Healthcare) using an Akta pure (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... Nbs from PE were purified using IMAC Hi-Trap columns (GE Healthcare, 17092003) following manufacturer’s instructions and eluted using 300 nM Imidazole ...
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bioRxiv - Microbiology 2023Quote: ... The F341_RBP was purified using Ni2+- NTA-affinity chromatography (His GraviTrap, GE Healthcare) according to manufacturer’s instructions and exchanged into PBS buffer using 10 kDA Amicon® Ultra-15 Centrifugal filter units ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was applied to a 5 ml His-TRAP column (GE Healthcare) and eluted with a gradient of 20 mM to 500 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: ... HIS-p300 was further purified on a Q sepharose column (HiTrap, GE Healthcare) in BC buffer and eluted between 100-600 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was loaded onto a nickel-chelated Hi-trap column (GE Healthcare) and eluted with a linear gradient of 25-500 mM imidazole in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2024Quote: ... The NRP2-his was purified using a HiTrap IMAC FF Ni2+ (GE Healthcare) affinity column and Superdex 75 10/300 size exclusion chromatography (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 μL lysate (2 mg protein) was injected through a 0.75mm loop (GE Healthcare 18-1112-53). Run parameters were 0.5 mL/min and 1.5MPa for 1 column volume ...
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bioRxiv - Cell Biology 2022Quote: ... PVDF membranes (GE Healthcare) were used for protein transfer ...
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bioRxiv - Neuroscience 2023Quote: ... Nitrocellulose membranes (GE Healthcare) were used for the transfer of proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... nylon membrane (GE Healthcare) at 65°C overnight ...
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bioRxiv - Immunology 2024Quote: ... PVDF membranes (GE Healthcare) were soaked for 1 min in methanol ...
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bioRxiv - Cell Biology 2019Quote: ... GST tags were cleaved with PreScission protease per the manufacturer’s instructions (GE Healthcare). Isolated CFP1 fragments were dialyzed in 25% glycerol ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was cleaved by the addition of PreScission Protease (GE Healthcare) with rocking incubation overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... the GST-tag was removed by proteolytic cleavage with PreScission Protease (GE Healthcare), followed by a final polishing step of the proteins by size-exclusion chromatography on an Äkta Purifier System using either HiLoad 26/600 Superdex 200 or HiLoad 26/75 Superdex columns (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The N-terminal His6 tag was removed by digestion with thrombin (GE Healthcare) by adding the enzyme at a concentration of 10 Units per mg of KsgA protein during overnight dialysis against PBS ...
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bioRxiv - Plant Biology 2020Quote: ... coli and purified using Ni and glutathione sepharose beads (GE Healthcare). Site mutagenesis of RBOHC and RBOHD was performed with a QuickChange Kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... coli lysates using glutathione-Sepharose 4B (GE Healthcare Amersham, Piscataway, NJ) and nickel affinity resin (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: ... coli cells were transformed with pGEX6 bacterial expression constructs (GE Healthcare) harboring GST-SBP (streptavidin binding peptide)-RGG or the two KH domains from mouse FMRP ...
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bioRxiv - Plant Biology 2022Quote: ... coli were performed according to the manual provided by GE healthcare. Briefly ...
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bioRxiv - Biophysics 2023Quote: ... coli cells and purified using GSH-Sepharose affinity chromatography (GE Healthcare) following by the size-exclusion chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3) and purified by Glutathione Sepharose 4B (GE Healthcare) as described previously (Nagamori et al. ...
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bioRxiv - Biochemistry 2024Quote: ... 45 μl was spotted to a double-membrane system on a dot-blot apparatus with an upper nitrocellulose membrane and a lower hybond-N+ membrane (GE Healthcare). After filtration under vacuum ...
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bioRxiv - Immunology 2021Quote: ... After washing the membranes with TBST, protein bands were detected with a chemiluminescent substrate (BIOMAX, USA) through a CCD Imager (GE life sciences, USA).
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bioRxiv - Developmental Biology 2019Quote: ... 30 μg of proteins from lysates were subjected to migration on 8–12 % acrylamide gels and transferred on to polyvinylidene difluoride membranes (GE Healthcare Europe GmbH) in borate buffer (50 mM Tris-HCl and 50 mM borate ...