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Citations for GE Life Sciences :
1151 - 1200 of 6651 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The lysates was subjected to Nickel gravity column using 5 ml His-Trap columns (GE Healthcare), and protein was eluted with 300 mM imidazole in column volumes ...
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bioRxiv - Microbiology 2020Quote: ... His-SUMO-NahA filtered supernatant was purified using a 5 mL HisTrap FF column (GE Healthcare) equipped on an ÄKTA FPLC apparatus (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... the clarified cell-free extracts were loaded onto a His-Trap FF crude column (GE Healthcare) preequilibrated with buffer B containing 0.1% (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... His-SEPT2-mCherry-SEPT6/7-strep was further purified using an AKTA FPLC system (GE Healthcare) with a Superdex 200 10/300 GL (Amersham Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were further purified using a 1 or 5 ml Hi-trap Q column (GE Healthcare) that was equilibrated in (100 mM NaCl,10 mM KH2PO4 ...
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bioRxiv - Biochemistry 2021Quote: ... an initial IMAC step was performed on a His-Trap column (all columns by GE Healthcare) using buffers A (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified by affinity chromatography on a 5 ml His-Trap FF crude column (GE Healthcare), the protein was further purified by gel filtration using a HiLoad Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... supernatants filtered through 0.45 µm syringe filter and loaded onto His Trap (5 mL, GE Healthcare) equilibrated with 5% HisB buffer (100% HisB ...
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bioRxiv - Biochemistry 2023Quote: ... His-GCN4-FCT was further purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Cell Biology 2022Quote: ... the elution fractions containing His-ZNF524 were further purified on a HiTrap Heparin HPTM (GE Healthcare). Therefore ...
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bioRxiv - Molecular Biology 2023Quote: ... mv4Int was purified on nickel-nitrilotriacetic acid affinity resin (1ml His-trap HP, GE Healthcare, USA). Column equilibration was performed by injecting 10 column volumes of buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... 2015) and transferred to a nitrocellulose membrane (Amersham Protran; 0.45-μm pore nitrocellulose membrane; GE Healthcare) using a Trans-Blot device (Bio-Rad ...
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bioRxiv - Biophysics 2022Quote: ... Sandwich the membrane-gel-membrane assembly between two sheets of Whatman paper (GE Healthcare #3030-917). Dry this assembly between paper towels for 1hr ...
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Synaptic Zn2+ potentiates the effects of cocaine on striatal dopamine neurotransmission and behaviorbioRxiv - Neuroscience 2021Quote: ... Slides were placed on BAS-IP SR 2040 E Super Resolution phosphor screens (GE Healthcare) for 14-days and imaged using a phosphor imager (Typhoon FLA 7000 ...
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bioRxiv - Biochemistry 2024Quote: ... After overnight exposure to a storage phosphor screen (BAS-IP MS 2025 E GE Healthcare) the signal was detected using a Typhoon FLA7000 phosphoimager.
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bioRxiv - Plant Biology 2020Quote: ... electroblotted to PVDF membrane (GE Healthcare), blocked in 5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Synthetic Biology 2019Quote: ... transferred to nitrocellulose membrane (GE Healthcare), and immunoblotting was performed using anti-GFP-HRP (sc-9996 HRP ...
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bioRxiv - Cancer Biology 2019Quote: ... transferred to PVDF membranes (GE Healthcare). The PVDF membranes were blocked with 5% nonfat dry milk for 1 hour at RT and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... Hybond positively charged membrane (GE Healthcare), and gel dryer filter paper (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Hybond-N membranes (Amersham/GE Healthcare) were used for Southern blots (45) ...
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bioRxiv - Neuroscience 2020Quote: ... transferred to nitrocellulose membranes (GE Healthcare), blocked with 5% milk ...
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bioRxiv - Biochemistry 2020Quote: ... HyBond positively charged membrane (GE Healthcare) and 0.45 μm Tuffryn membrane filter paper (PALL Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... transferred onto nitrocellulose membranes (GE Healthcare) and blocked in 5% non-fat milk in PBS with 0.1% Tween 20 ...
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bioRxiv - Biochemistry 2020Quote: ... electrotransferred to nitrocellulose membrane (GE Healthcare), and immunoblotted as indicated ...
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bioRxiv - Biochemistry 2020Quote: ... HyBond positively charged membrane (GE Healthcare), thick filter paper (Bio-Rad gel dryer filter paper) ...
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bioRxiv - Microbiology 2022Quote: ... transferred onto nitrocellulose membranes (GE Healthcare) and blocked in 5% non-fat milk in PBS with 0.1% Tween 20 ...
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bioRxiv - Microbiology 2021Quote: ... Nitrocellulose membranes (GE Healthcare Life Sciences) were probed with anti-GFP (clones 7.1 and 13.1 ...
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bioRxiv - Cell Biology 2020Quote: ... transferred to nitrocellulose membranes (GE Healthcare) and subjected to western blot analysis as described above.
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bioRxiv - Plant Biology 2022Quote: ... electroblotted to PVDF membrane (GE Healthcare), blocked in 5% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... or PVDF membrane (GE Healthcare #10600023) in cold (∼4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... electroblotted to PVDF membrane (GE Healthcare), blocked in 5% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... Hybond positively charged membrane (GE Healthcare), and gel dryer filter paper (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... or PVDF membranes (GE Healthcare # 10600023). Blocking was performed for 1h using 5% dried milk or Bovine Serum Albumin (Sigma-Aldrich #A9647 ...
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bioRxiv - Cancer Biology 2024Quote: ... transferred to nitrocellulose membranes (GE healthcare), immunoblotted with specific primary and secondary antibodies and detected by chemiluminescence with the ECL detection reagents from Thermo Fisher or Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... blotted onto nitrocellulose membrane (GE Healthcare), followed by blocking for 1 h in 5% non-fat milk and an incubation with primary antibodies at 4 °C under constant shaking overnight ...
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bioRxiv - Immunology 2019Quote: ... His10-tag free CD80 ectodomain was purified with a StrepTrap HP column (GE Healthcare, catalog 28907547) in 100 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by addition of PreScission Protease (GE Healthcare), and the BRPF1 bromodomain was eluted with wash buffer ...
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bioRxiv - Neuroscience 2021Quote: ... was cloned into the pGEX-6P-1 vector with an N-terminal GST tag (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... with an N-terminal glutathione transferase (GST) tag followed by a PreScission Protease site (GE Healthcare). The codon optimized cDNA for the ATAD2 BRD was designed by BioBasic and subsequently amplified by PCR and cloned into the pDest15 vector ...
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bioRxiv - Biochemistry 2024Quote: ... consisting of a Protran nitrocellulose membrane (Whatman) followed by a Hybond N+ nylon membrane (GE Healthcare Bioscience). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... coli strain Rosetta (DE3)pLysS and purified using Glutathione-Sepharose 4B beads (GE Healthcare). Membrane lipid strips (Echelon Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... coli BL21 and purified by Glutathione beads as per the manufacturer’s instructions (GE healthcare). The purity of the GST tagged proteins was assessed with denaturing SDS-PAGE followed by Coomassie blue staining ...
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bioRxiv - Neuroscience 2020Quote: ... coli and purified using a HisTrap FF Ni-NTA column (GE Healthcare Life Sciences). The SUMO tag was cleaved by incubation with SUMO protease (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... coli expressed SDAg6 to CNBr-Activated Sepharose 4 Fast Flow (GE Healthcare Life Sciences) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 cells from the pDYH24 plasmid and purified with glutathione sepharose (GE Healthcare). PUD-1/PUD-2 heterodimers were purified on a HisTrap column followed by gel filtration ...
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bioRxiv - Molecular Biology 2024Quote: ... coli cell fluorescence was carried out using a Typhoon FLA 9500 fluorimager (GE Healthcare) as described in Olorunniji et al ...
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bioRxiv - Cell Biology 2023Quote: ... coli extracts on 100 μl Glutathione–Sepharose beads in PBS (GE Healthcare, Freiburg, Germany). After incubation for 1h at 4°C with either E.coli extracts or purified proteins ...
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bioRxiv - Biochemistry 2020Quote: Proteins (200 μl at 2 μM) were separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in PBS ...
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bioRxiv - Biochemistry 2019Quote: ... analytical gel size-exclusion chromatography was performed with 1-2 mg protein in buffer A on a Superdex 75 10/300 column (GE Healthcare), to which a static light-scattering (SLS ...