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Citations for GE Life Sciences :
751 - 800 of 6458 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... pGEX-2T (GE Healthcare, N-terminal fusion with GST) was used for CPK protein production in bacteria for previously reported constructs72 (CPK2/3/5/11) ...
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bioRxiv - Genetics 2021Quote: ... DNA was transferred in 2 x SSC to positively charged nylon membranes (GE Healthcare Amersham Hybond-N+) and was then immobilized by ultraviolet cross-linking (1200 J) ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples (20 µg) were separated on 15% SDS-PAGE and transferred to Protran nitrocellulose membranes (GE Healthcare). Membranes were blocked overnight at 4°C with 5% milk in TBS-T (20 mM Tris pH 7.6 ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amount of 5-10µg proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). Membranes were revealed using a chemiluminescent HRP substrate (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare; Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” (Perkin Elmer ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were solubilized in 1% (w/v) LMNG and proteins were purified using a StrepTrap column (GE Healthcare). The resin was washed in TBS containing 0.01% (w/v ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were separated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane (GE Healthcare Life science). Equal loading was validated using Coomassie Brilliant Blue staining of the membrane ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was analyzed by SDS-PAGE and proteins were transferred to a nitrocellulose membrane (GE Healthcare, #BA85). Membranes were incubated for 1 h in a blocking solution (Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were resolved using SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare Life Sciences, cat#10600001) as previously described in [8] ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were resolved by SDS-PAGE and transferred to Protran nitrocellulose membranes (GE Healthcare Life Sciences, Waukesha, WI), which were incubated at 4°C overnight with primary antibodies ...
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bioRxiv - Biophysics 2020Quote: ... Protein samples were resolved on the SDS-PAGE and transferred onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare). The membrane was blocked with 5% non-fat dry milk in tris-buffered saline ...
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bioRxiv - Immunology 2020Quote: Cell lysates (10μg protein) were resolved by electrophoresis and transferred to a polyvinylidene fluoride membrane (GE Healthcare, UK). Membranes were blocked for 1 hour in 5% skimmed milk/TBS/0.1% Tween-20 and incubated with primary antibodies (1:1000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... The protein extracts were separated by SDS-PAGE and were subsequently transferred to Amersham Protran membranes (GE Healthcare) in a tankblot device (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of proteins were resolved in Tris-acetate SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). For extracellular vesicle fractions ...
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bioRxiv - Cancer Biology 2021Quote: ... We subjected equal amounts of proteins to SDS-PAGE and transferred them to nitrocellulose membranes (GE Healthcare #10401197). Non-specific binding was blocked by incubation with TBST plus 5% non-fat milk ...
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bioRxiv - Cell Biology 2019Quote: ... for the detection of ubiquitinated protein aggregates or a 0.2 μm pore size cellulose acetate membrane (GE Healthcare) for the detection of huntingtin aggregates using a 96 well format Dot-Blot apparatus (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: Proteins resolved by SDS PAGE were transferred to a nitrocellulose transfer membrane (GE Healthcare Amersham™ Protran™) at 80 V for 75 minutes or 15 V overnight at 4°C using a Bio-Rad wet transfer apparatus ...
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bioRxiv - Plant Biology 2020Quote: Thylakoid proteins were separated by SDS-PAGE and then transferred to a polyvinylidene membrane (Hybond P, GE Healthcare) using a tank blot system (Perfect Blue Web M ...
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bioRxiv - Cell Biology 2021Quote: ... protein extracts were subject to SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL). Primary antibodies used were anti-P2Y1 (Santa Cruz ...
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bioRxiv - Cancer Biology 2022Quote: ... After protein fractionation by SDS-page samples were transferred to a nitrocellulose membrane (cat#10600002 from GE healthcare) and incubated with primary antibody solutions overnight at 4 °C (p21 ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein was loaded and separated in SDS-page gels and then transferred to PVDF membrane (GE Healthcare). For blocking ...
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bioRxiv - Bioengineering 2023Quote: ... S17 were prepared by blotting tau and α-synuclein proteins onto nitrocellulose membrane (GE Healthcare Life Science, 10600004). Human HT40 fibrils (Stressmarq ...
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bioRxiv - Microbiology 2023Quote: Sample protein content present on the SDS-PAGE was transferred onto a polyvinylidene difluoride (PVDF) membrane (GE Healthcare) for 90 min (25 V ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated on 10 % or 14% SDS-PAGE and transferred to nitrocellulose membranes (#10600002, GE Healthcare Biosciences). The membranes were blocked with 1% BSA in TBS-Tween 20 (TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS/PAGE in 10% gel before wet transfer to 0.45μm nitrocellulose membranes (GE Healthcare) and blocked in 5% dry milk powder in TBS-T (100mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to a nitrocellulose membrane with a 0.2 μm pore size (GE Healthcare Life Sciences, 10600001) by wet transfer at 100 V for 120 minutes ...
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bioRxiv - Plant Biology 2023Quote: Thylakoid proteins were separated by SDS-PAGE and then transferred to a polyvinylidene membrane (Hybond P, GE Healthcare) using a tank blot system (Perfect Blue Web M ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins were separated by 8-10% SDS-PAGE and transferred to a Hybond ECL nitrocellulose membrane (GE Healthcare) or Amersham Protran 0.45 μm NC nitrocellulose membranes (Carl Roth ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Molecular Biology 2022Quote: ... was purified and washed on His-Trap columns (GE Healthcare) with buffer Ac (50 mM Tris pH 7.9 ...
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bioRxiv - Biophysics 2022Quote: ... and purified over a His-Trap HP column (GE Healthcare). The proteins were gel-filtered and their size was confirmed via a Superdex 200 column on an AKTA Pure system (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... A His-Trap crude FF column (GE Healthcare Life Sciences) was used for this purification ...
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bioRxiv - Biochemistry 2019Quote: ... coli and coupled to CNBr-beads (GE Healthcare). The specificity of custom-made antibodies was confirmed by western blotting against human cell lysate after RNAi depletion of target proteins as described previously (Cota et al. ...
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bioRxiv - Biochemistry 2023Quote: ... coli and purified using ÄKTA pure (GE Healthcare) with HiTrap Blue HP ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were dried for 2-3 hours at 30°C and exposed to a Tritium Phosphor Screen (GE Healthcare, Fuji BAS-TR 2025 E) together with calibrated tritium standards (American Radiolabeled Chemicals ...
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bioRxiv - Biochemistry 2022Quote: ... The GST tag was cleaved with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The tags were cleaved off by PreScission protease (GE Healthcare) and the proteins were further purified by sizeexclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2023Quote: ... The GST tag was cleaved by PreScission Protease (GE Healthcare) treatment over 12 hrs at 4°C with the progress of reaction monitored by SDS-PAGE ...
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bioRxiv - Immunology 2020Quote: ... MERS-CoV S1 was purified over a Superdex200 Increase column (GE Life Sciences). SARS-CoV-2 S-2P ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using the 2-D Quant kit (GE Life Sciences 80648356). Samples were reduced (5 mM DTT ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted protein solution was quantified using the 2-D Quant Kit (GE Healthcare) following the manufacturer’s instruction.
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bioRxiv - Microbiology 2022Quote: ... Membranes were developed with ECL 2 western blot substrate and scanned with an ImageQuant LAS 4000 (GE Healthcare), and signals analyzed with ImageQuant TL 7.0 software (GE Healthcare).
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bioRxiv - Developmental Biology 2020Quote: ... The protein were electrophoresised for 1h at 150V and subsequently transferred onto MtOH activated polyvinylidene fluoride membranes (GE Healthcare). Equal loading was assessed using Ponceau red solution ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were separated by SDS-PAGE and transferred to AmershamTM ProtranTM 0.45 μm nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were probed with primary antibodies at dilutions recommended by the suppliers ...
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred from polyacrylamide to nitrocellulose membranes (AmershamTM ProtranTM Premium 0.45 µm NC, GE Healthcare Life sciences, 10600003) by electrotransfer (Bio-Rad laboratories Inc.) ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences). The following antibodies were used ...
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bioRxiv - Cell Biology 2019Quote: ... 40 μg of total protein were run on a SDS-polyacrylamide gel and transferred onto nitrocellulose membranes (GE Healthcare). After blocking in 5% (w/v ...
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bioRxiv - Cell Biology 2019Quote: For Western blotting protein samples were resolved by 10% SDS-PAGE and transferred to a PVDF membrane (GE Healthcare) by semi-dry blotting ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The respective IgG2a antibodies were incubated with the PepSpot membrane at concentrations ranging from 0.5 µg/ml to 2µg/ml and detected by protein A-HRP (GE Healthcare).